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... correspond to the hole injection barrier, does not in fact reflect the true injection barrier Chapter extends these measurements to blue light-emitting diodes based on poly(fluorene-alttriarylamine) ... 2.2.3 Photodiode bandwidth calibration Three photodiode detectors are used to cover the spectrum range from eV to 0.5 eV The range is based on the consideration that π - π * band-gap for most ... injection barrier 3.1 Introduction The physics of charge-carrier injection through traditional metal–semiconductor (MS) contacts is dominated by Schottky barrier formation.[1] The zero-field barriers...

Ngày tải lên: 14/09/2015, 08:38

... pcDNA3.1(-) (Invitrogen Corp., Carlsbad CA) Mutation of cysteine residues C53, C63, and C91 in human BST-2, either alone or in combination, to alanine was accomplished by PCR-based mutagenesis of pcDNA-BST-2 ... Triton-X100) and incubated on ice for minutes After lysis, the cells were pelleted at 13,000 × g for minutes to remove insoluble material The supernatants and the virus lysates were incubated on a rotating ... T, Kobata A: Cell surface glycoprotein and asparaginelinked sugar chain patterns of rat erythroleukemic cell lines Cancer Res 1982, 42:2884-2893 Kaletsky RL, Francica JR, Agrawal-Gamse C, Bates...

Ngày tải lên: 12/08/2014, 23:22

... structure-based drug design for Alzheimer's disease by considering the 3D-pharmacophoric features of the complex responsible for discriminating these two closely-related cholinesterases Background ... http://www.tbiomed.com/content/7/1/22 the more specific binding of Compound A to BChE This study may provide a rational basis for the structure-based design of benzothiazepine drugs, in combination with a selected docking protocol, ... Schulten K: VMD - Visual molecular dynamics J Mol Graph Model 1996, 14:33-38 48 Pilger C Bartolucci C, Lamba D, Tropsha A, Fels G: Accurate prediction of the bound conformation of galanthamine...

Ngày tải lên: 13/08/2014, 16:20

... Helicobacter pylori cagA subtype infection J Clin Pathol 2001; 54: 703-706 26 Hamajima N, Shibata A, Katsuda N, et al Subjects with TNF-A-857TT and -1031TT genotypes showed the highest Helicobacter ... Graduate School of Medicine Tests for Helicobacer pylori (H pylori) antibody and pepsinogens Anti-H pylori IgG antibody tests, high-molecularweight campylobacter-associated-protein (HM-CAP) ELISA ... DM, Bray F, Ferlay J, Pisani P Global cancer statistics, 2002 CA Cancer J Clin 2005; 55:74-108 Blaser MJ, Perez-Perez GI, Kleanthous H, et al Infection with Helicobacter pylori strains possessing...

Ngày tải lên: 31/10/2012, 15:37

... activation by DNA-IgG complexes Epithelial cells Increased enterocyte permeability causing barrier dysfunction Bactericidal effects Platelets Expressed on cell surface by activated platelets Osteoclasts ... sepsis Trends Mol Med 2005, 11:56-63 41 Bonaldi T, Talamo F, Scaffidi P, Ferrera D, Porto A, Bachi A, Rubartelli A, Agresti A, Bianchi ME: Monocytic cells hyperacetylate chromatin protein HMGB1 ... Originally called p30, this protein was renamed amphoterin because of its content of both acidic and basic residue segments The sequence for amphoterin matches the sequence of HMGB1, establishing HMGB1...

Ngày tải lên: 09/08/2014, 10:23

... [6] COX-2-dependent prostaglandin E2 production was measured after incubating cells with LPS/IFN for 24 h Cells were then incubated in fresh medium containing 20 μM arachidonic acid, LPS, IFN and ... GAC TGG ATT CTA TGG TG, and antisense, 5'-AGG AGA GGT TGG AGA AGG CT from Genbank accession BC052900, producing a 263 base pair product Half of each reaction was electrophoresed in 1% agarose Gels ... porcine μ-calpain, in calpain reaction buffer, incubated 30 min, and then denatured for western blotting A representative blot of COX-2 is shown Bars represent the average COX-2 signal intensity...

Ngày tải lên: 11/08/2014, 08:22

... NA ≥1 exacerbation in the past years Never or ex-smokers for >6 months Cotinine checked every weeks Never or ex-smokers for >6 months Cotinine checked at 1, 6, 24 and 30 months Exacerbations Smoking ... CT Change in PD15 measured by CT Exacerbations Lung function (FEV1, KCO) Quality of life (SGRQ) Other endpoints AAT: alpha-1 antitrypsin; EXACTLE: Exacerbations and Computed Tomography scan as ... variable was included in the integrated analysis database to identify the records in the Danish-Dutch or EXACTLE studies All CT scan analyses were based on the modified intent-to-treat (ITT) population,...

Ngày tải lên: 12/08/2014, 11:22

... Barbaro G: Metabolic and cardiovascular complications of highly active antiretroviral therapy for HIV infection Curr HIV Res 2006, 4:79-85 Chun TW, Stuyver L, Mizell SB, Ehler LA, Mican JA, Baseler ... reservoirs might be feasible through global cellular activation [146-148] This strategy is termed immune activation therapy (IAT) The achievability of cytokine-based IAT was proven in vitro with a ... Hexamethylene bisacetamide (HMBA) is a clinically tolerable agent [267], first developed as an anticancer drug, which could be of interest in the reactivation of the latent reservoirs HMBA causes the release...

Ngày tải lên: 12/08/2014, 23:22

... of the biotinylated MA (MA-BAD) from extracts of 293 cells mock-transduced (Mock) or transduced with the MABAD vector (293 MA-BADv), or from lysed MA-BAD vector (MA-BADv) analysed by Western blotting ... attempts to capture IN-BAD from lysates of infected cells Instead, we mixed lysates obtained from IN-BAD particles (IN-BADv) and HEK 293 cells (293ctl or 293sh) and asked whether IN-BAD could be captured ... 293 cells mock-transduced (lane 1) or transduced with the IN-BAD vector (293 IN-BADv) (lane 2, 4) or INQ168A-BAD vector (293 INQ168A-BADv (lane 3, 5) analysed by Western blotting with the anti-IN...

Ngày tải lên: 12/08/2014, 23:23

... the protease background determines the level of maturation inhibitor resistance and viral replication Results In vitro selections To assess the impact of different PI resistant backgrounds on ... viral replication in different genetic backgrounds Therefore we introduced Gag mutations V362I, A364V, S368N or V370A by site-directed mutagenesis in the background of HXB2, PR-1 and PR-2 The ... the PR-2 background Again, mutations A364V and V370A conferred > 150-fold resistance but, interestingly, mutations V362I and S368N, which demonstrated only low-level resistance in the background...

Ngày tải lên: 13/08/2014, 01:21

... and suppression of glucagon secretion, as well as slowing gastric emptying [13] The latter is probably the dominant mechanism to account for glucose lowering after oral or intragastric meals in ... 2004, 32:848-851 Sokos GG, Bolukoglu H, German J, Hentosz T, Magovern GJ Jr, Maher TD, Dean DA, Bailey SH, Marrone G, Benckart DH, Elahi D, Shannon RP: Effect of glucagon-like peptide-1 (GLP-1) ... Kolterman OG, Buse JB, Fineman MS, Gaines E, Heintz S, Bicsak TA, Taylor K, Kim D, Aisporna M, Wang Y, Baron AD: Synthetic exendin-4 (exenatide) significantly reduces postprandial and fasting plasma...

Ngày tải lên: 13/08/2014, 21:21

... capacity 1.1 Several Basic Problems of Probability Theory This section presents the basic theory of probability includes the concept of random variable, distribution, probability density function ... been published in local and abroad related to the following issues: - Theoretically: The basic research on probability theory, study of the algorithms to generate random variables, methods, models ... variance, skewness coefficient and the kurtosis coefficient, and as a basis for further study 1.2 The Gamma Distribution 1.2.1 The Probability Density Function A continuous random variable X is said...

Ngày tải lên: 05/09/2016, 23:25

... for bacterial respiratory enzymes [15] More extensive associations between the respiratory chain complexes, the so-called ‘respirasomes’, have recently been found in mammals, plants and bacteria ... wild-type strain in which three high molecular mass bands were clearly detected (Fig 1A) It was previously shown that these three protein bands in the complex from wild-type yeast correspond ... absence of the Qcr10p subunit, only the two higher molecular mass bands were detected by BN ⁄ PAGE analysis, but not the 670 kDa band corresponding to the homodimeric bc1 complex (Fig 2A) This...

Ngày tải lên: 18/02/2014, 08:20

... Kobayashi T, Hotta Y & Tabata S (1993) Isolation and characterization of a yeast gene that is homologous with a meiosis specific cDNA from a plant Mol Gen Genet 237, 225–232 15 Kobayashi T, Kobayashi ... Acknowledgements We would like to acknowledge Dr S.K Apte and Dr A.S Bhagwat, Molecular Biology Division, BARC, Mumbai, India for critical reviewing of this manuscript References Kowalczykowski SC, Dixon DA, ... Hotta Y, Miyajima N, Tanaka A & Tabata S (1994) Characterization of cDNAs induced in meiotic prophase in lily microsporocytes DNA Res 1, 15–26 16 Sato S, Hotta Y & Tabata S (1995) Structural analysis...

Ngày tải lên: 19/02/2014, 07:20

... error bars, are based on measurement of three individual cultures FEBS Journal 272 (2005) 2292–2303 ª 2005 FEBS 2297 Control analysis of the las operon Comparison of control by the las enzymes Based ... (5¢-CTAGTCTAGATGAGCTCCAGAAGCTTCC-3¢) were amplified The PCR products were digested with XhoI ⁄ BamHI and BamHI ⁄ XbaI, respectively, and cloned in identical restriction sites in plasmid pG+host8, using ... selected strains (including flux control coefficients) Standard deviations, indicated by error bars, are based on measurement of three individual cultures Fig Flux control coefficients for the las...

Ngày tải lên: 19/02/2014, 17:20

... dissolution of the empire, to barbarism; which conquered the barbarians; which became the bond, the means, the principle of civilisation to the Roman and the barbarian world."[27] In this passage, ... dissolution of the empire and the barbarian invasion It did break down later in Asia and in all north Africa beneath an invasion of the same kind that of barbarous Mussulmans It broke down then ... Gundebald commander of the forces, but died himself in October of that same year, 472, and left the throne to be the gift of barbarian adventurers Three more shadows of emperors passed Gundebald...

Ngày tải lên: 21/02/2014, 11:20

... human F5 clone described previously [19,20] Enzyme assay Enzyme aliquots were incubated with 0.2 mM Ala-Ala-PhepNA (Bachem, Bubendorf, Switzerland) in 0.1 M potassium phosphate buffer, pH 7.5, containing ... of 200 lL The rate of change in absorbance at 405 nm was measured in a Multiscan PLUS ELISA plate reader (Labsystems, Helsinki, Finland) [21] A molar absorbance of 9600 M)1Æcm)1 for pNA was used ... fragments in the constructs are indicated by filled and open bars, respectively The fragment originating from the rat gene is indicated by a hatched bar The activity in cell extracts of stable transformants...

Ngày tải lên: 21/02/2014, 15:20

... authors claim that regions with high expected probability of Software for controlling formation of bacterial inclusion bodies formation of backbone–backbone hydrogen bonds as well as regions with ... different probability scales for the 20 amino acid residue types, acting separately either as donors or acceptors of backbone–backbone hydrogen bonds, calculated from the same database of 3769 ... multiple alternative folding patterns and assigns relative a priori probabilities based solely on amino acid sequence, probability tables and pre-chosen parameters In the foldamyloid method [36],...

Ngày tải lên: 06/03/2014, 00:20

... previously established 16 h to be an appropriate incubation time period, as the activity vs time graph still shows linearity After 16 h of incubation, the steroids were extracted twice with mL of ... to produce androstadienol after overnight incubation (16 h) (C) HEK-293 cells transfected with lg pCMV-cyt b5 and 0.1 lg human P450c17 were incubated with nM of [3H]preg in the absence/ presence ... [3H]preg in the absence/ presence of lM ketoconazole Metabolites were analyzed after overnight incubation (16 h) (D) Nonlabeled standards revealed with molybdate/sulfuric acid (10 : 10, v/v) P450,...

Ngày tải lên: 08/03/2014, 08:20

... database available from http://www.medgen.med.ualberta.ca/database.html 21 Cox, D.W (1999) Disorders of copper transport Br Med Bull 55, 544–555 22 Roberts, E.A & Cox, D.W (1998) Wilson disease Bailliere’s ... The 67-kDa band, marked with F, is an N-terminally cleaved proteolytic fragment of the ATPase, persistently present despite the use of protease inhibitors The faint high molecular mass band may ... (Eur J Biochem 269) each membrane batch used for the assays described below The genomic ZntA gene is not expressed under our growth conditions [26] A major band at 80 kDa represents a ZntA monomer,...

Ngày tải lên: 17/03/2014, 17:20