uv visible vis near infrared nir spectroscopy

... (O'Byrne 2007) In 2004, asthma exacerbations resulted in 14.7 million outpatient visits, 1.8 million emergency room visits, 497000 hospitalizations and 4055 deaths in the United States alone While ... Tao Lin 22 Jan 2014 ii ACKNOWLEDGEMENT First and foremost, I would like to deeply thank my supervisor Professor Wong Wai-Shiu Fred for his guidance and assistance through my Ph.D studies Without ... been divided into ten genera: ebterovirus, cardiovirus, aphthovirus, erbovirus, hepatovirus, kobuvirus, parechovirus, teschovirus and rhinovirus Human rhinoviruses, like other picornaviruses,...

Ngày tải lên: 09/09/2015, 11:16

... activity in rats with adjuvant arthritis Ann Rheum Dis 1983, 42:693-698 27 Gilman SC, Daniels JF, Wilson RE, Carlson RP, Lewis AJ: Lymphoid abnormalities in rats with adjuvant-induced arthritis ... aggravates, rat adjuvant arthritis Arthritis Rheum 1996, 39:204-215 S4 Kinne RW, Schmidt-Weber CB, Hoppe R, Buchner E, PalomboKinne E, Nỹrnberg E, Emmrich F: Long-term amelioration of rat adjuvant arthritis ... Castell M: Treatment with an anti-CD4 monoclonal antibody strongly ameliorates established rat adjuvant arthritis Clin Exp Immunol 1996, 103:273-278 12 Billingham MEJ, Hicks CA, Carney SL: Monoclonal...

Ngày tải lên: 09/08/2014, 03:24

... helix-loop-helix transcription factors Myf-5 and MyoD J Biol Chem 268, 9869–9878 37 Tapscott, S.J., Davis, R.L., Thayer, M.J., Cheng, P.F., Weintraub, H & Lassar, A.B (1988) MyoD1: a nuclear phosphoprotein ... region to convert fibroblasts to myoblasts Science 242, 405–411 Ó FEBS 2004 38 Lassar, A.B., Davis, R.L., Wright, W.E., Kadesch, T., Murre, C., Voronova, A., Baltimore, D & Weintraub, H (1991)...

Ngày tải lên: 07/03/2014, 16:20

... state transitions process Under conditions of balanced light illumination of photosystems, a linear photosynthetic electron flow (solid arrows) is favoured resulting in the generation of reducing ... transition), favouring energy redistribution towards PSII In Chlamydomonas, a switch between linear (State 1) and cyclic (State 2) electron flow around PSI occurs Observed migration of the cyt ... aid of a redox-active tyrosine Yz and a catalytic centre composed of four Mn ions and a Ca ion Linear electron transfer proceeds with the soluble electron carrier plastocyanin, which undergoes...

Ngày tải lên: 16/03/2014, 06:20

... found that ATP binding and hydrolysis are important for their interaction in Saccharomyces cerevisiae [12] Recently, it has been reported that the PEX1–PEX6 complex is involved in the dissociation ... low-energy value (< ) 10 kcalÆmol)1; shown in red in supplementary Fig S2) In contrast, the areas near R135 in PEX1-ND and the corresponding region around R144 in VCP-ND displayed no significant ... L175, L176 and I177, is positioned vertically across the surface of the groove, with R135 and K174 near one end (Fig 2A) The surfaces where the hydrophobic patch FEBS Journal 273 (2006) 4959–4971...

Ngày tải lên: 16/03/2014, 12:20

... Arg125 side chains are shown in grey circles Distances between the cytosine methyl groups and the nearest Thr44 and Arg125 side chain atoms are indicated by arrows (Right) Interaction between CENP-B ... reconstituted by the method shown in panel A, was analyzed by 20% nondenaturing PAGE Bands were visualized by ethidium bromide staining methylated DNA complex model To so, we employed a complex-reconstitution ... A Discussion B Fig CpG methylation reduces the affinity between CENP-B and the CENP-B box DNA to nearly the level of nonspecific DNA binding (A) The 32P-labeled CENP-B box DNA (1 lM) was mixed with...

Ngày tải lên: 16/03/2014, 18:20

... reverses the mutant yeast phenotype and SBPmediated specific sucrose uptake in yeast displays linear, nonsaturable kinetics up to 30 mM external sucrose, being relatively insensitive to pH gradient ... biochemical features closely resemble the kinetics properties of the previously characterized linear component of sucrose uptake in higher plants [18–20] Recently, we have conducted overexpression ... SBP-mediated sucrose transport in yeast has been characterized biochemically and displays nonsaturable, linear uptake Fig GTP-binding of S64/SBP2 (A) N-Terminal His-tagged S64 fusion protein of the wild-type...

Ngày tải lên: 23/03/2014, 21:21

... Spectroscopic methods UV ⁄ visible spectra were recorded at room temperature in a Shimadzu UVPC-1601 spectrometer (Shimadzu, Kyoto, Japan) equipped with cell stirring Fluorescence spectroscopy was ... suggested to be relevant for protein– protein interactions However, because W155 packs against a nearby arginine (R165), its mutation to an arginine results in a repulsive interaction arising from ... and (D) I154F The spectra were recorded at 600 MHz and 25 °C (Fig 2B–D) This is confirmed by far -UV CD because the spectrum of the mutants is overall identical to that of the wild-type frataxin...

Ngày tải lên: 30/03/2014, 04:20

... Raussens et al [8] investigated the structural organization of apoE3-NT in DMPC complexes by infrared spectroscopy These authors presented a model wherein the NT domain adopts an open conformation, ... FEBS K A Redmond et al at +20 kV and masses were detected in linear mode with Protein A used as external calibrant Fluorescence spectroscopy Fluorescence spectra were obtained using a PerkinElmer ... limited ability to protect LDL from PL-C induced turbidity development while HT apoE3NT conferred nearly full protection These studies were extended by evaluating the effect of apolipoprotein concentration...

Ngày tải lên: 30/03/2014, 11:20

... fashion in rat adjuvant-induced arthritis model (a) Male Lewis rats were injected with coma dose-dependent fashion in rat adjuvant-induced arthritis model plete Freund's adjuvant on day and maximal ... C-reactive protein (CRP) as well as the inhibition of adjuvant-induced gene expression in the spleen, liver, and popliteal lymph nodes in the rat adjuvant arthritis model Moreover, WAY-169916 was also ... differences were deemed significant if P < 0.05 Rat adjuvant-induced arthritis model Arthritis was induced in the Lewis rats with complete Freund's adjuvant (CFA) by intradermal injection of 0.1 mg of...

Ngày tải lên: 09/08/2014, 06:22

... the decreased expression of endothelial cell adhesion molecules [16] and paw swelling in a rat adjuvant arthritis model [17] Further studies searching for alternative therapeutic strategies against ... addition of CpG 2216 and cultured for three hours (A, B) Freshly isolated or activated pDCs were visualized by immunofluorescence with IRF7 antibody (Cy5, red) and nuclei staining with DAPI (blue) ... University, 1-7-29, Suehiro, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan Received: 15 February 2010 Revised: 27 April 2010 Accepted: 14 May 2010 Published: 14 May 2010 ArthritisMiyamoto et al.; licensee...

Ngày tải lên: 12/08/2014, 12:20

... Dear Parent/Caregiver , The National Association of Pediatric Nurse Practitioners (NAPNAP) and our nearly 7,000 pediatric nurse practitioners are dedicated to helping parents understand and meet ... bag races, hopscotch, or tire races • Taking the stairs – see how many you can climb in one day! Visit www.napnap.org to learn more Healthy Foods Choose A V ariety! • Vegetables • Fruits • Milk ... blue vegetables meat and beans grains fruits milk Answers on page 12 For more on the Food Pyramid, visit www.mypyramid.gov Dot’s Delicious! Connect the dots to find R.B.’s favorite kind of food! He...

Ngày tải lên: 27/10/2014, 18:03

... backpack, jacket or other gear in the way of the equipment • Always have adult supervision at the playground Have FUN! Visit www.napnap.org to learn more Play it Safe! Going to the playground is fun ... Dear Parent/Caregiver , The National Association of Pediatric Nurse Practitioners (NAPNAP) and our nearly 7,000 pediatric nurse practitioners are dedicated to helping parents understand and meet ... playing safely Can you find them all? Answers on page 11 For more information on playground safety, visit http://www.nsc.org/library/facts/plgrdgen.htm Staying Safe Online This activity is for you...

Ngày tải lên: 27/10/2014, 18:03

... Dear Parent/Caregiver , The National Association of Pediatric Nurse Practitioners (NAPNAP) and our nearly 7,000 pediatric nurse practitioners are dedicated to helping parents understand and meet ... you can share it with others! Top Tooth Tips: • • • • Brush at least twice a day Floss every day Visit the dentist regularly Fight plaque by limiting the number of times you eat snacks Tip: Try ... breakfast • Brush before bedtime • Brush every tooth, inside and out • Don’t forget your tongue! • Visit your dentist regularly Toothy Teaser Keep smiling as you test your skill with this mind-teaser...

Ngày tải lên: 27/10/2014, 18:03

... or elution by a linear gradient from 93 : to 70 : 30 (v/v) CH3CN/H2O in 20 at a ow rate of 1.0 mLặmin)1 PNPG1-7 and 4-nitrophenol were detected at 313 nm (Shimadzu SPD-10AU UV- VIS detector) and ... glutathionylation in the lower pI form [38,5052] The aromatic replacements Met53Trp/Tyr had no visible activity in the zymogram and exchange by Asp (Fig 2) and Glu (not shown) did not signicantly ... containing 1.34% yeast nitrogen base, 0.4 lgặmL)1 biotin, 0.5% methanol, and 1% soluble starch to visualize secreted active a-amylase by exposure to I2 vapor [51] Production and purication of enzyme...

Ngày tải lên: 31/03/2014, 08:20

... wheat thaumatin-like xylanase inhibitor (TLXI) PhD thesis, pp 186 Katholieke Universiteit Leuven, Leuven (Belgium) 33 Pollet A, Vandermarliere E, Lammertyn J, Strelkov SV, Delcour JA & Courtin ... purification was typically 20–50 mgÆL)1 culture XAN PmeI (New England Biolabs, Beverly, MA, USA) linearized pPicZaC-exlA was used to transform P pastoris strain X33 by electroporation Extracellular ... measurements were X6, Xylazyme AX, Azo-wheat AX, water-unextractable AX and OSX X6 is a soluble, linear, oligomeric substrate Xylazyme AX and Azo-wheat AX are polymeric chromophoric AX that are water-unextractable...

Ngày tải lên: 14/02/2014, 19:20

... not conducted to preserve anomeric ratios generated by the enzyme, one important trend is still visible: the combination of a relative predominance of b-anomers for the (GlcNAc)2 product and the ... USA) The release of 4MU proved to be linear with time for all substrate concentrations, allowing the straightforward calculation of enzyme velocities by linear regression (all curves had correlation ... and (GlcNAc)4 ( ) by LlChi18A, illustrated by the production of (GlcNAc)2 during the initial linear phase of the degradation reaction Note that the enzyme concentrations used in the two reactions...

Ngày tải lên: 18/02/2014, 08:20

... products are visible intracellularly and pericellularly (white arrow) Addition of the 2A2 mAb to the assay medium reduced the degradation of DQ-collagen IV (B) Degradation products are visible intracellularly ... SPR EPGYSP is located between amino acids 133–138 at the exposed part of the cathepsin B molecule near the occluding loop (Fig 2C) The location of the epitope indicates that the binding of 2A2 mAb ... at room temperature 0.05% (Sigma) and 0.09% H2O2 in 0.05 m Tris-HCl buffer (pH 7.5) were used to visualize the spots SPR The binding kinetics of 2A2 mAb to cathepsin B were determined by the SPR-based...

Ngày tải lên: 18/02/2014, 11:20

... and near -UV CD spectroscopy The native state of the recombinant proteins as well as structural changes upon calcium-binding were monitored by far- and near -UV CD spectroscopy (Fig 7) Far -UV CD ... significant change in the near -UV CD spectra of both proteins upon titration with calcium, corroborating our results of far -UV CD and Trp fluorescence spectroscopy 2D NMR spectroscopy Each crosspeak ... 0, 0.5, and mM to bA3-crystallin Fig Near -UV CD spectroscopy: (A) 1.1 mgÆmL)1 of bB2- and (B) 0.65 mgÆmL)1 of bA3-crystallin was used for recording the farUV CD spectra Calcium was added from...

Ngày tải lên: 18/02/2014, 16:20

... thrombotic process [2–4] Once thrombin is formed, it will inevitably interact with and activate all nearby platelets [5] Thrombin stimulates platelets mostly or only via the protease-activated receptors,...

Ngày tải lên: 18/02/2014, 16:20