using genetic manipulation to study molecular basis of physiological responses

... induces apoptosis in a Bax-dependent manner Inhibitor of apoptosis protein family Induction of MOMP following apoptotic stimuli results in the release of cytochrome c and subsequent activation of caspase-9 ... of Bax activation in response to a variety of apoptosis-inducing stimuli Molecular mechanisms of sensitivity to EGFR-TKIs JNK and mitogen-activated protein kinase phosphatase-1 The activity of ... is characterized by the permeabilization of the outer mitochondrial membrane and the release of several pro-apoptotic factors into the cytoplasm For mitochondrial outer membrane permeabilization...

Ngày tải lên: 16/02/2014, 09:20

... Preface The purpose of The Molecular Basis of Plant Genetic Diversity is to provide a glimpse into the dynamic process of genetic variation by presenting the thoughts of some of the scientists ... potato and tomato Fig The symptoms of late blight (Phytophthora infestans) infection in potato leaves and tubers Photos kindly provided by Dr Zsolt Polgỏr Prior to the arrival of Phytophthora ... application of molecular population genetics, phylogenetics and genomics to answer ecological questions These different disciplines are united in an attempt to study genetic- based questions of ecology,...

Ngày tải lên: 28/06/2014, 11:20

... Baseline to mos 7.2 2.0 to 25.9 to 12 mos 1.5 0.2 to 9.5

Ngày tải lên: 10/08/2014, 10:23

... as models to further our understanding of cellular metal toxicology, and because of the importance of metal toxicity in microbial biotechnologies [4-7] Chromium toxicity is an issue of especially ... expected to have a primary protein target - the toxic metal chromium Prior to our study intense efforts to characterize the toxic action of chromium have been made, but the primary molecular ... concept of loss -of- function of an essential protein target (the focus of the drug-induced haploinsufficiency studies [14,15,21,24]) could be less relevant to modeof-action than toxic gain -of- function,...

Ngày tải lên: 14/08/2014, 08:20

... analysis of MD simulation results, the average structures of the protease-inhibitor complexes of the last 100 ps of the simulations were used, superimposing backbone Cα atoms On the basis of these ... compared based on the differences of the intramolecular distances using the backbone carbon alpha (Cα) atoms and the geometric centers of the side chain atoms [35] In total, 37 experimental models ... constraints of a predefined covalent bond The covalent bonding was observed for analogous ketoamide inhibitors [25] and ketoacid inhibitors [12] We used MOE (Molecular Operating Environment) [41] to visualize...

Ngày tải lên: 14/08/2014, 08:20

... frequency of each QTL For the scenarios of equal QTL variance, variance of each QTL was set to For the scenarios of unequal QTL variance, variance of every tenth QTL was set to 81 and variances of the ... coefficient of determination of a QTL, expressed as R , is the proportion of variance of that QTL explained by a set of markers R2 was calculated using the equation R2 = c’K-1c, where c is a vector of ... number of QTL ranging between the intermediate and high number of QTL and using an unequal distribution of QTL variance to investigate the strong decrease of accuracies of BM from scenario to scenario...

Ngày tải lên: 14/08/2014, 13:21

... The idea of using short stories as supplementary materials came to her when she read the M.A thesis of Nguyen Thi Ngoc Tu, “A study on using short stories to improve the efficiency of teaching ... them to discuss what they think of the story 33 Procedure: - Select a story and hand it out to the students in advance - Tell the story to the students (or play the story for them to listen to) ... difficulties of the language?  Even if the language of the story is extremely difficult, will students be motivated by other factors to study it (e.g students often enjoy studying a difficult short story...

Ngày tải lên: 28/03/2015, 08:54

... noninhibitory conformation Glyphosate sensitivity is partly restored by mutation of Ala100 to glycine, allowing glyphosate to bind in its extended, inhibitory conformation Detoxification of glyphosate ... action of glyphosate and the molecular basis for glyphosate resistance Structure and function of EPSPS EPSPS catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the ... of this enzyme, performed by various laboratories over the past three decades This review summarizes some of the key findings that have led to our current understanding of the molecular mode of...

Ngày tải lên: 14/02/2014, 14:20

... Diseases to expose the enrolled study participants to the inferior treatment or to offer the possibility of treatment assignment to an inferior treatment for new subjects to be accrued To make ... Family/Social History The family history and social history may elicit genetic factors or other triggers that might cause the development of lung disease The family history is an important source of information ... different types of study designs; some of which require only a fraction of the number of subjects required to the randomized controlled trial, which is often considered the gold standard To begin,...

Ngày tải lên: 05/03/2014, 23:20

... molecular approach to dominance in hypophosphatasia Hum Genet 109, 99–108 Molecular basis of perinatal form of hypophosphatasia 31 Brown DA & Rose JK (1992) Sorting of GPI-anchored proteins to ... compilation ª 2008 FEBS 2731 Molecular basis of perinatal form of hypophosphatasia N Numa et al Fig Molecular properties of the TNSALP (V406A) mutant Established CHO-K1 TetOn cells harboring a plasmid ... transport of the mutant protein was similar to that of the wild-type protein, as assessed by the acquisition of Endo H resistance Besides, the mutant protein was found to be incorporated into the...

Ngày tải lên: 07/03/2014, 05:20

... cell and using the cell’s molecular machinery Structure of T2 phage DNA & protein Life cycle of virulent T2 phage: Hershey-Chase Bacteriophage Experiment - 1953 T2 bacteriophage is composed of DNA ... phosphodiester bonds to form polynucleotides Phosphodiester bond Covalent bond between the phosphate group (attached to 5’ carbon) of one nucleotide and the 3’ carbon of the sugar of another nucleotide ... Model of DNA Two sources of information: Base composition studies of Erwin Chargaff • indicated double-stranded DNA consists of ~50% purines (A,G) and ~50% pyrimidines (T, C) • amount of A =...

Ngày tải lên: 13/03/2014, 16:34

... cell and using the cell’s molecular machinery Structure of T2 phage DNA & protein Life cycle of virulent T2 phage: Hershey-Chase Bacteriophage Experiment - 1953 T2 bacteriophage is composed of DNA ... phosphodiester bonds to form polynucleotides Phosphodiester bond Covalent bond between the phosphate group (attached to 5’ carbon) of one nucleotide and the 3’ carbon of the sugar of another nucleotide ... Model of DNA Two sources of information: Base composition studies of Erwin Chargaff • indicated double-stranded DNA consists of ~50% purines (A,G) and ~50% pyrimidines (T, C) • amount of A =...

Ngày tải lên: 13/03/2014, 17:04

... the failure of the toxin to trigger glutamate release from toxin-treated mouse brain synaptosomal Molecular basis of toxicity of C perfringens e toxin fractions In this study, synaptosomal preparations ... support to the idea that they have a similar function to e-toxin In addition, there is a similar level of Molecular basis of toxicity of C perfringens e toxin sequence identity to a number of putative ... to use this toxin as a bioterrorist weapon, and highlights the need to understand the molecular basis of toxicity in order to develop an effective vaccine Molecular biology of e-toxin The e-toxin...

Ngày tải lên: 14/03/2014, 22:20

... students about the storage and realization of genetic information As a sounding illustration of this topic, one of the Brandenburg Concertos was played and in parallel the single pages of the partitur, ... second (or further) receptor subunit is recruited into the complex, leading to the activation of the cytoplasmic receptor parts, e.g by transphosphorylation of inherent or receptor-associated kinases ... the type I receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMP ligand Therefore, the binding of the receptors of both subtypes is...

Ngày tải lên: 15/03/2014, 09:20

... CONACyT-Mexico grants 83084 to ES, 80534 to RMS and ‘Acuerdo del Rector General’ UAM to AR References Reeves RE (1984) Metabolism of Entamoeba histolytica Schaudinn, 1993 Adv Parasitol 23, 105–142 McLaughlin ... packages, using as templates the tertiary structures from yeast [15] and pig [16,17] PGKs, because of their higher levels of Table Nucleotide specificities of E histolytica and S cerevisiae PGKs in cytosolic-enriched ... geometry of the EhPGK models proved to be essentially identical to those of template structures, the variability in side-chain orientations allowed us to propose some mutants which might respond to...

Ngày tải lên: 16/03/2014, 01:20

... mutations that result in a loss of functional activity The object of this study was to increase the solubility of the C-terminal domain of AMPP, and in so doing to determine which residues are ... digestion of the AMPP gene (pepP) The gene fragments gave rise to a series of protein fragments that were examined for their solubility by fusing them to DHFR and monitoring the absence or presence of ... digestion of the pepP gene A set of nested truncated pepP genes was fused to that of DHFR in the fusion vector pJWL1030folA and transformed into competent E coli cells Two libraries of about 10...

Ngày tải lên: 23/03/2014, 07:20

... employing cytoPrP transgenic mice [15], it was shown that toxicity correlates with membrane localization of cytoPrP [19] In a different study, apoptotic effects were linked to the association of cytoPrP ... lead to significantly increased generation of Ctm PrP In addition, several pieces of evidence have linked CtmPrP to neurodegeneration in transgenic mice Toxicity of transmembrane isoforms of PrP ... ataxia owing to cerebellar degeneration and gliosis [15] Cytotoxic effects of cytoPrP were also observed in some cell culture models [15–19], whereas in other studies the expression of cytoPrP seemed...

Ngày tải lên: 30/03/2014, 09:20

... proved to be important in developing our understanding of the role of the phytochromes in light-regulated plant development [13] As all phytochromes appear to use the same chromophore, this class of ... the recovery of phytochrome responses in pcd1 is that additional HOs are functional at this developmental stage Indeed, additional HOs have been shown to contribute to phytochrome responses in ... would also account for the failure of the PsHO1 probe to detect any HO2-like sequences in Southern blot experiments Whatever the basis of the recovery of phytochrome responses in older pcd1 plants,...

Ngày tải lên: 30/03/2014, 11:20

... to be infertile and subsequent histological examination of testes revealed greatly reduced amount of germinal epithelium and total absence of ongoing spermatogenesis resembling a syndrome causing ... the conversion of spermidine to putrescine while spermine is readily converted to spermidine in the absence of SSAT [6] The conversion of spermine back to spermidine in the absence of SSAT activity ... at years of age, which is close to the end of the life-span of a mouse [42] According to a recent report, spontaneous tumor incidence in old mice of CH3 background (used in the cited study) is...

Ngày tải lên: 30/03/2014, 13:20

... draft of the human genome, one of the first priorities was to develop a large-scale map of common genetic variation to investigate the role of genetics in human disease (1–3) Using the catalog of ... genetic tests, to monitor population use of genetic tests and to determine the impact of genetic information on the health and well-being of different populations The results of such studies ... Gagnon, MSc, PhD University of Toronto Dalla Lana School of Public Health Toronto, ON, Canada Montserrat Garcia-Closas, MD, MPH, DrPH Division of Cancer Epidemiology and Genetics National Cancer...

Ngày tải lên: 11/06/2014, 10:43