... waiver of written informed consent (# 09-953) Redbloodcell transfusion characteristics Packed redbloodcell units were obtained from the blood bank (St John’s Mercy Medical Center) None of the ... transfusion is one of the most commonly used interventions in the ICU to treat severe anemia, which often occurs in sepsis In the United States, more than 14 million units of packed redblood cells (RBCs) ... Age of transfused blood is an independent risk factor for post injury multiple organ failure Am J Surg 1999, 178:570-572 47 Ho J, Sibbald WJ, Chin-Yee IH: Effects of storage on efficacy ofred cell...
... transfused fresh and old RBCs RBC: redblood cells more units at RBCs transfused of 28 or more days of storage in patients transfused or The relation between in-hospital mortality and the amount of ... cryoprecipitate, percentage of RBCs that were leukoreduced, mechanism of Page of 11 (page number not for citation purposes) injury, useof DVT prophylaxis, ICU free days, and cause of death The GCS recorded ... mortality and the amount of RBC units transfused at 28 or more days of storage in patients transfused or more units of RBCs RBC: redblood cells Page of 11 (page number not for citation purposes)...
... purposes of the analysis of metabolic changes during CPB, only the storage time of the blood used for priming the CPB machine was considered, and the composition of the two groups therefore differed ... range 0.27 to 0.65 0.27 to 0.54 Transfused blood Duration of storage (days) Number ofredbloodcell units 0.985 Demographic features Age (months) Male sex no of patients (%) 0.089 Weight (kgs) ... were used in the priming solution of the CPB circuit were included in the bloodprime group The useof RBCs in the priming solution is a current practice at our institution in cases when the use of...
... human redblood cells J Theor Biol 1996, 179:329-368 Ni TC, Savageau MA: Application of biochemical systems theory to metabolism in human redblood cells Signal propagation and accuracy of representation ... E -Cell system version 1.1 was used as the simulation platform in this work The software can be downloaded from http://www.e -cell. org/ Our prototype model of the RBC was developed on the basis of ... also generates NADPH This reduction power is employed by various other intracellular processes, in particular the reduction of GSSG A major function of GSH in Page of 11 (page number not for...
... of the cells to the center of the tube in a process known as redbloodcell axial migration (40, 58, 103, 140) Axial migration of the redbloodcell is induced by the “tank-treading” motion of ... heterogeneity ofredblood cells distribution in the network by triggering the effects ofredbloodcell screening (103) and plasma skimming (53, 122) shown in Fig 1.10 CELL SCREENING Cell- cell interactions ... SKIMMING Cell- free layer Side branch Figure 1.10: Redbloodcell screening and plasma skimming at an arteriolar bifurcation The black ellipsoid represents a redbloodcellRedbloodcell screening...
... WSS ofblood samples at 40% Hct in microtubes of ID 30 µm, 50 µm and 100 µm in non-aggregating blood 73 Figure B2 Comparison of relative WSS ofblood samples at 40% Hct in microtubes of ... Hct Hematocrit (Hct) is the ratio of the volume ofredblood cells (RBCs) to the total blood volume Hct is well known to be a major rheological determinant ofblood viscosity and flow resistance ... whole blood viscosity measured in viscometer due to the presence of cell- free layer as discussed above, but higher than the plasma viscosity because of the protrusion of RBCs into the cell- free...
... and useof the blood conservation device The same variables were entered into a separate logistic regression model to ascertain the independent predictors of ICU and hospital mortality We used ... of Hb, it is logical that useof such a device will reduce transfusion requirements In this beforeand-after study, useof a blood conservation device in the presence of a restrictive transfusion ... standardised restrictive transfusion practice can reduce the number of units of PRBC transfused The secondary objective is to investigate if the useof the device is associated with a smaller decrease...
... European Consensus Conference definition of ARDS [20] and relied upon the presence of PaO2/FiO2 (arterial partial pressure of oxygen/fraction of inspired oxygen) of less than or equal to 200 mm Hg, ... Kristiansson M, Soop M, Saraste L, Sundqvist KG: Cytokines in stored redbloodcell concentrates: promoters of systemic inflammation and simulators of acute transfusion reactions? Acta Anaesthesiol Scand ... effects of transfusion-associated immunomodulation: fact or fiction? Blood 2001, 97:1180-1195 30 Lee TH, Stromberg RR, Heitman J, Tran K, Busch MP: Quantitation of residual white cells in filtered blood...
... with Cell Quest and FloJo Analysis Software Cell fusion assays 293T cells were co-transfected with pHRSVFOptA2 and pBD-NFκB (effectors cells), and the panel ofcell lines from a variety of different ... A) Comparison of fusion activity of wild-type and a fusion peptide mutant of HRSV F Figure A) Comparison of fusion activity of wild-type and a fusion peptide mutant of HRSV F 293T cells co-transfected ... GFP Infection of hamster BHK-21 cells also resulted in a time dependent increase in the number of GFP positive cells, although the appearance of a large number of bright GFP positive cells seemed...
... question of the effect of RBC storage on clinical outcomes: The RedCell Storage Duration Study (RECESS) (Section 7) Tranfus Apher Sci 2010, 43:107-116 Ness PM: Does transfusion of stored redblood cells ... for blood banks [65,66] Question of leukoreduction Many of the adverse effects associated with the transfusion of allogeneic RBCs have been shown to be related to the infusion of white blood cells ... to reduce blood losses, for example useof antifibrinolytic agents, such as tranexamic acid or epsilon-aminocaproic acid (EACA) and techniques ofcell salvage during surgery; also, the use of...
... mg mLÀ1 of the red cells), most of which is hemoglobin (335 mg mLÀ1 of the red cells), lipids (5.1 mg mLÀ1 of the red cells), and carbohydrates (80 mg mLÀ1 of the red cells) The redcell membranes ... pathogenesis of hereditary spherocytosis appeared to be related to possible 1.3 The Dawn ofRedCell Membrane Research abnormalities in the redcell membrane constituents It is well known that redcell ... Elucidation of the Pathogenesis ofRedCell Membrane Disorders In parallel with the investigation of normal redcell membrane protein biochemistry, or a little bit later, the disease states ofred cell...
... Equation is used to determine OD value ofcell biomass N’ = X*10n (3) Where N’ is the amount of cells in the sample X is the total cells of sample that was calculated standard curve of various ... 0C 30 Concentration of total sugar % (w/w) 12 Mass of consumed sugar g 9.2 Mass ofcell g 1.0 Time of fermentation h 72 Re -use time Storage of stability at 40C day 10 Mass of alcohol g 4.69 Fermentative ... exchanged for gram m (g) is sample of weight 2.2.2.Measurement ofcell biomass concentration [6] [7] Thomas’ cell counting chamber Equation is used to count the numbers ofcell in biomass N = [((a/b)*400)...
... specificity of serum SCC-Ag in predicting tumor recurrence The results reported here and those of others indicate that routine surveillance of serum SCC-Ag during follow-up can be used to predict ... cell carcinoma antigen; PPV = positive predictive value; NPV = negative predictive value IIB III/IV Histology – No (%) Squamous cell carcinoma 102 (91.1) Adenocarcinoma ( 6.2) Adenosquamous cell ... performed ROC analysis of ΔSCC-Ag to determine the optimal levels for predicting the probability of tumor recurrence (Figure 2) The area under the ROC curve of ΔSCC-Ag, which is considered to indicate...
... (≤6 units) blood product exposure and reduced long-term survival [3] As the risk of transfusion-associated adverse outcomes may depend on the amount of transfusion [4], reduction ofblood transfusions ... A (62 of 100 patients) compared to group B (75 of 92 patients, p < 0.001) Intraoperatively, 81 patients were transfused, receiving 142 units of PRCs The number of intraoperative PRC Page of 10 ... transfused, receiving 289 units of PRCs, and the total number of PRC units transfused was significantly lower in group A (113 units) compared to group B (176 units, p < 0.0001) The percentage of...
... advantage of DBS samples over routine blood samples is that only a small quantity of blood, typically 50 μl, is required to make one dried blood spot They are easy to obtain, stable for long periods of ... 20 The supernatant was stored at -70 degree C until use Replicate sets of DBS samples were prepared dropping 50 μl of whole blood in each circle (5 spots per card) of the filter paper (SS&S903, ... DBS at: -20 Figure of Stability the dried blood samples Stability of the dried blood samples PCR amplifications of HBV DNA from DBS 1% Agarose gel showing a 322 bp fragment of the HBV-"a determinant"...
... Logistic Organ Dysfunction; RBC, redblood cell; SAPS, Simplified Acute Physiology Score Page of (page number not for citation purposes) We examined the characteristics of patients discharged from ... previous history of anaemia, especially in the setting of neoplastic disorder Page of (page number not for citation purposes) Table Multiple logistic regression analysis of predictive factors ... LOD, Logistic Organ Dysfunction; OR, odds ratio; RBC, redbloodcell One of the most important factors associated with post-ICU transfusion of RBC was sepsis as an admission diagnosis A previous...
... interferes with the release of O2 for use by tissues [74] Risks ofredbloodcell transfusion Even if anemia is harmful, this does not necessarily prove that liberal useof allogeneic RBCs to normalize ... storage on redbloodcell rheological properties J Surg Res 2002, 102:6-12 Tsai AG, Cabrales P, Intaglietta M: Microvascular perfusion upon exchange transfusion with stored redblood cells in norTransfusion ... since the implementation of universal leukoreduction in many jurisdictions [84] It has been suggested that the useof fresher blood might further minimize the risks of transfusion, while also...