use of human mesenchymal stem cells as alternative source of smooth muscle cells in vessel engineering

Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon interaction ... differentiation of rat bone marrow-derived mesenchymal stem cells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stem cells using basement...

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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf

Ngày tải lên : 09/08/2014, 10:23

... the basis of the expression of alkaline phosphatase (ALP) activity and incorporation of calcium in the extracellular matrix The deposition of a mineralized matrix was further examined by staining ... Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during ... osteogenic medium Induction of an medium osteogenic phenotype of mesenchymal stem cells (MSCs) cultured in osteogenic media versus basal media, as demonstrated by alkaline phosphatase staining (Neutral...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Ngày tải lên : 09/08/2014, 14:22

... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... Aggregates were incubated with 50 μL of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, ... immunohistochemical analyses, sections were washed for 20 minutes in Tris-buffered saline (TBS), and incubated in 5% BSA (Sigma, St Louis, MO, USA) Following washing in TBS, Page of 15 (page number not for citation...

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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Ngày tải lên : 10/08/2014, 05:21

... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... widespread interest in finding alternative sources of b -cells for tissue replacement strategies in diabetes MSCs have been used for cell-based therapy in regenerative medicine and tissue engineering In ... LAM increases insulin release after glucose challenge To quantify functional insulin release by stage IV cells, we used glucose-challenge test and assayed with a human insulin ELISA A baseline...

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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps

Ngày tải lên : 12/08/2014, 15:22

... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression of ... http://arthritis-research.com/content/12/6/R210 Page of 13 Figure Effects of UC-MSCs on the invasive behavior and IL-6 production of FLSs in vitro (a) Invasive behavior of FLSs in Matrigel matrix was measured in the transwell system Forty-eight ... of the invasive phenotype of FLSs [32] Therefore, we further investigated the effect of UC-MSCs on the invasive behavior of FLSs by the Cell Migration/Invasion Assay, and the MMP9 secretion of...

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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells

Ngày tải lên : 09/09/2015, 10:07

... Ability of Human Mesenchymal Stem Cells during Serial Passage Using Nanofibrous Scaffolds Proceedings of International Conference on Materials for Advanced Technologies (ICMAT), 26/06 01/07/2011, Singapore ... scaffolds by electrospinning, and the sustained release of this drug can induce the osteogenic differentiation of MSCs investigate the influences of prolonged culture as well as the nanofibrous structure ... Tetranectin Binds to plasminogen; may regulate matrix mineralization RGD-containing glycoproteins Thrombospondins Cell attachment (I, II, III, IV) Fibronectin Binds to cells Vitronectin Cell-attachment...

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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

Ngày tải lên : 09/09/2015, 10:16

... Generating Vascularised Tissue-Engineered Bone Grafts: Endothelial Progenitor Cells in Vasculogenic and Osteogenic Priming of Human Fetal Mesenchymal Stem Cells International Bone-Tissue -Engineering ... Tissue Engineering 1.4.1 Limitations in Bone Tissue Engineering 1.5 Importance of Vascularisation 1.5.1 Vascularisation in Bone Tissue Engineering 1.5.2 Vascularisation ... Tissue Engineering To overcome the issues encountered in current strategies, bone tissue engineering (BTE) has emerged as an alternative for fracture repairs Tissue engineering is “an interdisciplinary...

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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... emas eht gnisu dezitehtsena erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof ... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...

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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

Ngày tải lên : 10/08/2014, 10:21

... such as ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of ... growth inhibitory effect of mesenchymal stem cells on tumor cells in vitro and in vivo Cancer Biol Ther 2008, 7(2):245-51 79 Piscaglia AC, Campanale M, Gasbarrini A, Gasbarrini G: Stem Cell-Based ... volume of 20 μl was prepared, of which μl of starting RNA material was included for RT-PCR, a final concentration of 0.5 μM of each forward and reverse primer and 0.2 μM of the TaqMan probe was used...

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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf

Ngày tải lên : 12/08/2014, 14:22

... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... Curcumin concentrations as low as 0.5 μM suppresses IL-1β induced activation of caspase-3 and COX-2 Higher concentrations of curcumin completely inhibited IL-1β induced activation of caspase-3 ... and was blocked in all cultures treated with IL-1β and curcumin Here, a four-hour pre-treatment with curcumin was as effective in inhibiting the IL-1β induced apoptotic signalling cascade as a...

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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot

Ngày tải lên : 12/08/2014, 15:23

... outcome measures including use of gene profiling with a custom PCR array of 42 genes associated with a healthy NP phenotype, an important innovation of this study, as well as assessments of GAG, ... Calcein staining the cytoplasm of live cells green and ETH-2 staining the nuclear envelope of dead cells red Histology of pellets To visualize the intact pellet, pellets were first fixed in formalin ... searched against the International Protein Index (IPI) non-redundant protein database using Sequest; requiring tryptic specificity; allowing precursor m/z tolerances of Da; allowing methionine residues...

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Báo cáo y học: "Mesenchymal stem cells in arthritic disease" pot

Ngày tải lên : 09/08/2014, 13:22

... demonstrated in vivo The first of such studies was carried out in baboons in which systematic administration of allogeneic MSCs was used to prolong skin graft [47] In an animal model of experimental ... Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice ... Interactions between human mesenchymal stem cells and natural killer cells Stem Cells 2006, 24:74-85 52 Ramasamy R, Fazekasova H, Lam EW, Soeiro I, Lombardi G, Dazzi F: Mesenchymal stem cells inhibit dendritic...

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Báo cáo y học: " Mesenchymal stem cells in autoimmune diseases: hype or hope" ppt

Ngày tải lên : 12/08/2014, 14:21

... disease course [1,8] The latter could in part be related to the use of allogeneic MSCs, as it has been reported that the use of allogeneic cells – presumably through additional cytokine release as ... diﬃculties in recapitulating the in vitro eﬀects in vivo, however, researchers should be cautioned and should remain critical concerning the use of MSCs for the treatment of human autoimmune disease ... experience with the use of MSCs in the setting of GVHD can help guide their use for rheumatic diseases, thereby certifying or revoking their therapeutic use for the control of autoimmunity Abbreviations...

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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Ngày tải lên : 30/03/2014, 04:20

... 1.00 ATP-binding cassette, sub-family transcript variant ATP-binding cassette, sub-family ATP-binding cassette, sub-family transcript variant ATP-binding cassette, sub-family ATP-binding cassette, ... apolipoprotein A-I apolipoprotein A-II apolipoprotein B apolipoprotein C-I apolipoprotein C-II apolipoprotein D apolipoprotein E apolipoprotein F apolipoprotein H fatty acid binding protein 1, liver lipase, ... markers such as E-cadherin and a-catenin were up-regulated in AT-MSC-Hepa In contrast, the expression of mesenchymal markers such as N-cadherin and vimentin was down-regulated (Table 4) During hepatic...

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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx

Ngày tải lên : 18/06/2014, 15:20

... possibility of using stem cells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular through non-invasive procedures ... myosin expression in muscle control and in the induced hFTs cells 1C) Myosin band observed in the muscle control and in the induced hFTs cells by Ponceau S membrane dyeing 1D) IF assay, indicating ... Results of hFTs lineage in passage two Panel B) Results of hFTs lineage in passage 11 We observed high rates of cell division, with gradual decreasing of the population doubling time (PDT) in lineages...

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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Ngày tải lên : 07/08/2014, 18:21

... sulp ASB/SBP ni detulid erew seidobitna ehT seidobitna yramirp fo gnidnib cificeps-non eht kcolb ot Co73 ta nim 001 rof )ASB/SBP( ASB %4 gniniatnoc SBP ni )ASU ,demyZ( mures taog %2 dna )ASU ,LRB-ocbiG( ... ecnanetniam eht dna euqinhcet reporp eht ,erutluc yramirp ot gnilpmas morf emit eht taht thguoht saw tI ]72,22[ srotinegorp citeiopotameah rof esac eht sa emas eht ,srotinegorp lamyhcnesem ni ... erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP htiw desnir erew )2 egassap doolb droc( sllec ,gnilebal PAFG dna ESN lacimehcotyconummi eht roF )ASU ,nocimehC ;PAFG(...

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Báo cáo y học: "Local adherent technique for transplanting mesenchymal stem cells as a potential treatment of cartilage defect" pdf

Ngày tải lên : 09/08/2014, 10:23

... and muscle MSCs [21] For clinical safety, autologous human serum should be used instead of FBS We recently reported that autologous human serum predominated in increasing the proliferation of human ... portion of the cells adhered to the cartilage defect Injection of more cells would increase the number of cells that adhered to the defect; however, the injection of a large number of cells would ... events during a 10-minute period (a) period Intercellular adhesion molecule (ICAM-1) expression in human synovial mesenchymal stem cells (MSCs) minute and 10 minutes after plating on glass slides...

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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf

Ngày tải lên : 10/08/2014, 05:21

... Page of Figure Immunostaining of anti-a-actinin and anti-a MHC in cells at different time points of culturing A strong expression of both a-actinin and MHC proteins (A, B, D, E, G, H, J, K) was ... fluorescent immunostaining of aactinin of cells from three groups, while, J-L shows the fluorescent immunostaining of MHC of cells from these groups after and 10 days’ culturing Cells from CMCM and ... undefined mechanisms Myocardial tissue engineering has now emerged as a promising treatment for heart diseases such as severe heart failure As a new transplantation therapy, “cell sheet engineering ...

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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx

Ngày tải lên : 10/08/2014, 10:20

... the Cell Quest software The result showed that 15.1 ± 2.9% of the cells was in S +G2/M phase (active proliferative phase) with the remaining cells in G0/G1 phase (quiescent phase, 84.9% ± 2.9%) ... differentiation of hADSCs Compared to non-induced control (A), Alkaline phosphatase staining was increased after being induced for weeks (B) (Bars = 100 μm) and ALP activity was quantified by ... Marshak DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage,...

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Báo cáo y học: " Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates " pps

Ngày tải lên : 12/08/2014, 13:22

... category was graded on a five point scale: = minimal injury, = injury up to 25% of the field, = Page of 11 injury up to 50% of the field, = injury up to 75% of the field, and = diffuse injury [19] ... cytokine profiles Lung homogenates at post-injury day were used for a mouse macroarray experiment to profile various inflammatory cytokine proteins involved in the peak inflammatory reactions induced ... macroarray Each lung lysate was analyzed using a mouse cytokine array kit (Proteome Profiler™; R&D Systems, Minneapolis, MN, USA) A total of 250 μg of lysate was incubated in the nitrocellulose membrane...

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