unravelling glycobiology by nmr spectroscopy
Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
Ngày tải lên :
16/03/2014, 18:20
... containing hCypA were clarified by ultracentrifugation (100 000 g, h) and stored at °C NMR spectroscopy All NMR spectra were recorded at 25 °C using a Varian INOVA 600 MHz NMR spectrometer equipped ... 10% (v/v) before NMR measurement Results Cell-free protein synthesis enhanced by T7 RNA polymerase Prior to the preparation of 15N-labelled samples of hCypA and analysis by NMR spectroscopy, the ... metabolic by- products were removed easily by dialysis of the sample prior to NMR analysis (data not shown) For proteins with a molecular mass similar to hCypA, dialysis can be achieved simply by transfer...
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Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx
Ngày tải lên :
23/03/2014, 18:20
... [14C/13C]phenylacetate were detected by HPLC They were subsequently analyzed by 13C -NMR spectroscopy with high selectivity and sensitivity because of the multiple 13 C-labeling In 1D 13C -NMR spectra, the signals ... 13C-labeled metabolites (Fig 3, Table 2), which were identified by 13C -NMR spectroscopy without prior purification This approach was possible by the sensitivity and selectivity enhancement due to multiple ... photometrically (260 nm) and by online liquidscintillation counting The retention times for phenylacetate, phenylacetyl-CoA and 2-hydroxyphenylacetate were 20, 21 and 12 min, respectively NMR spectroscopy The...
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Probing protein dynamics and protein protein interaction by NMR spectroscopy
Ngày tải lên :
11/09/2015, 10:17
... Transform NMR spectroscopy In 1971, Jeener proposed the idea of two dimensional (2D) NMR spectroscopy during a lecture, which was then realized by Aue et al (Aue et al 1976) The applications of 2D NMR ... necessary to review a general history and basic theories of NMR spectroscopy 1.1 History of NMR In 1890, prior to the discovery of NMR, Zeeman observed unusual behavior of certain nuclei in a ... structure determination approaches, NMR spectroscopy and X-ray crystallography, are facing more challenges to study systems with an increasing molecular weight For NMR spectroscopy approach, spectra...
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Mapping the binding site of ligands to proteins using chemical exchange parameters by NMR spectroscopy
Ngày tải lên :
14/09/2015, 08:49
... Mapping the binding site of ligands to proteins using chemical exchange parameters by NMR Spectroscopy NMR spectroscopy, along with X-ray crystallography, have advanced to such an extent that ... out by the author at the NMR Structural biology lab, the Department of Biological Sciences, Singapore This work has been supervised by Associate professor Dr Henry Mok and co-supervised by Associate ... exchange by definition, is the switching of environments by a nucleus occuring at a particular rate, whereas, relaxation is the process by which the nucleus gets back to its equilibrium state by losing...
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Structural and equilibrium unfolding studies of sam domain of DLC1 by NMR spectroscopy
Ngày tải lên :
14/09/2015, 14:04
... SAM domains 1.2 Protein structure determination by NMR spectroscopy 1.2.1 Fundamentals of NMR spectroscopy 1.2.1.1 NMR phenomenon 1.2.1.2 Basic NMR parameters 1.2.1.2.1 Chemical shift 1.2.1.2.2 ... of SAM domains 1.2 Protein structure determination by NMR spectroscopy Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy, is the technique which studies magnetic ... all NMR studies that have been done in the past few years, but rather serves to give readers a general idea of the basics of NMR spectroscopy and the protein structure determination by NMR spectroscopy...
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DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY
Ngày tải lên :
04/10/2015, 15:45
... sequences used in multidimensional NMR experiments Figure 1.5: Outline of the procedure for protein structure determination by NMR 15 Figure 1.6: Effects of protein size on NMR signals 17 Figure 2.1: ... detected in all NMR experiments On a modern NMR spectrometer, ν typically lies in the radio frequency (RF) range between 50 and 800 MHz for hydrogen nuclei The signal in NMR spectroscopy results ... conformational space allowed by the constraints For this reason, a structure solved by NMR is in fact a bundle of structures rather than a unique one, and its quality is assessed by the root-mean-square...
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Mapping of the binding surface between EPHA5 and antagonist peptide by NMR spectroscopy
Ngày tải lên :
16/10/2015, 15:38
... Characterization of WDC by CD 38 3.8 Structural Characterization of WDC by NMR 38 3.9 NMR Structure of WDC 42 3.10 Mapping of Binding Interface between EphA5 and WDC by NMR 46 3.10.1 ... X-ray crystallography and NMR spectroscopy can yield information at 11 atomic resolution 1.2.1 Introduction to NMR spectroscopy 1.2.1.1 NMR Nuclear magnetic resonance (NMR) is a property that magnetic ... EphA5-binding interface within WDC by NMR and ITC 46 3.10.1.1 Interaction of WDC-mutant peptides with EphA5 by NMR … 46 3.10.1.2 Interaction of WDC-mutant peptides with EphA5 by ITC……….50 3.10.1.3 Structural...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
Ngày tải lên :
19/02/2014, 12:20
... NMR spectroscopy Oligosaccharides were coevaporated with 2H2O (99.9 atom% 2H2) twice by lyophilization and dissolved in 500 lL high-quality 2H2O (100.0 atom% 2H2), containing 0.1 lL acetone NMR ... four structures by the combined use of ES-MS, methylation analysis and NMR spectroscopy The branching pattern and blood group-related Lewis determinants can be readily determined by high-sensitivity ... and one internal Lex determinants with the sequence: Completion of sequence assignment by 1H NMR Homonuclear NMR was carried out to verify the MS assignment of the iLNO core structures and its variously...
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Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
Ngày tải lên :
08/03/2014, 10:20
... kit (Pierce) NMR spectroscopy 1D 1H NMR and STD spectra were recorded with a Bruker Avance DRX-600 or DRX-500 spectrometer Standard Bruker software was used to acquire and process the NMR data Polysaccharide ... cases the molecular mass limit of the ligand for STD NMR spectroscopy can be extended This is the ®rst description of an application of STD NMR spectroscopy to identify the LPS epitope of a monoclonal ... transfer was investigated by recording STD spectra with k scans and saturation times from 0.25 s to s Relative STD values were calculated by dividing STD signal intensities by the intensities of...
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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
Ngày tải lên :
17/03/2014, 11:20
... zwitterionic lipid membranes determined by FTIR spectroscopy Figure depicts FTIR analysis of the two peptides in model membranes The FTIR analysis is aided by the NMR results, which allow assigning ... macromolecules by two-dimensional NMR experiments Biochem Biophys Res Commun 96, 1156–1163 26 Piotto, M., Saudek, V & Sklenar, V (1992) Gradient-tailored excitation for single-quantum NMR spectroscopy ... surface DISCUSSION Membrane binding and lysis by cytolytic peptides are influenced by their secondary structure In previous studies we have used FTIR spectroscopy to examine the membrane-bound structures...
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Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot
Ngày tải lên :
23/03/2014, 13:20
... M.W., Kaptein, R & Thornton, J.M (1996) AQUA and PROCHECK -NMR: Programs for checking the quality of protein structures solved by NMR J Biomol NMR 8, 477–486 30 Koradi, R., Billeter, M & Wuthrich, ... results of the spin system identification were confirmed by the 1H-13C HSQC spectrum The chemical shifts table of 13C -NMR for Ca and Cb and 1H -NMR has been deposited in BioMagResBank (BMRB), code ... Braswell, E & Dobson, C.M (1991) Solution structure of neuronal bungarotoxin determined by two-dimensional NMR spectroscopy: sequence specific assignments, secondary structure and dimer formation...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
Ngày tải lên :
30/03/2014, 20:20
... amidation Solution conformation by NMR spectroscopy and simulated annealing calculation The conformational features of EM2 and EM2OH, obtained by the present NMR measurements and molecular modeling ... previously compared the conformations of EM2 and EM2OH in dimethyl sulfoxide, as determined by 1H -NMR spectroscopy and molecular energy calculations, and reported [6] that: (a) substitution of ... influenced by the variation in pH, because the NMR spectra of EM2 in a solution of acidic pH were identical to those in solution of pH 5.2 This is in contrast with the EM2OH conformers, whose NMR spectra...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
Ngày tải lên :
31/03/2014, 21:21
... CD spectroscopy and 2D homonuclear/heteronuclear NMR spectroscopy Using these peptides, the interaction between the amphipathic domain of YopD and its cognate chaperone LcrH was investigated by ... proton correlation spectroscopy J Magn Reson 53, 521–528 25 Jeener, J., Meier, B.H., Bachmann, P & Ernst, R.R (1979) Investigation of exchange processes by two-dimensional NMR spectroscopy J Chem ... M.W., Kaptein, R & Thornton, J.M (1996) AQUA and PROCHECK -NMR: programs for checking the quality of protein structures solved by NMR J Biomol NMR 8, 477–486 ˚ Hakansson, S., Bergman, T., Vanooteghem,...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
Ngày tải lên :
20/02/2014, 02:22
... myristate-dependent conformational changes of the N and B states of Mn(III)heme–HSA, by optical absorbance spectroscopy and NMR spectroscopy, that show allosteric interaction(s) between FAs and Mn(III)heme ... myristate for binding to FA1 (defined by the parameters KH and KM) and on the allosteric modulation of FA1 properties by myristate binding to FAx (defined by the parameters KM*, K H M , and possibly ... contribution to the 17O -NMR linewidth at pH 7.0 and 10.0 as a function of myristate concentration (Fig 8) For paramagnetic metalloproteins, the width of the 17O NMR resonance is affected by the presence...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
Ngày tải lên :
21/02/2014, 01:21
... 15 16 17 ACKNOWLEDGEMENTS Supported by FONDECYT 1000756 and by NSERC of Canada 18 REFERENCES Utter, M.F & Kolenbrander, H.M (1972) Formation of oxalacetate by CO2 xation on phosphoenolpyruvate ... mM ATP plus mM Mn2+ (d) 0.05 mM PEP plus mM Mn2+ conformational changes caused by the ligand are easily sensed by the P-pyridoxyl chromophore Figure shows the modeled structure of the P-pyridoxyl-PEP ... dynamics Quenching experiments by acrylamide in the presence of several combinations of substrates and divalent ions at saturating concentrations were carried out by measuring the quenching of...
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Báo cáo khoa học: Influence of inflammation-related changes on conformational characteristics of HLA-B27 subtypes as detected by IR spectroscopy potx
Ngày tải lên :
06/03/2014, 00:21
... represented by red spheres Hydrogen bonds and salt bridges are depicted by dashed red lines (A) pVIPR-U5 drawn in purple as presented by B*2705 (B) pVIPR-U5 drawn in yellow as presented by B*2709 ... partners are indeed influenced Analysis of a T-cell receptor footprint on an HLA-A2:peptide complex by NMR spectroscopy [38] is a first step towards understanding this intricate issue, although the flexibility ... [2,3,11] The proteins encoded by these two alleles differ only by a micropolymorphism (Asp116 in B*2705 and His116 in B*2709) within the peptide-binding groove formed by each of the HC [1,3] Detailed...
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Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx
Ngày tải lên :
07/03/2014, 21:20
... of RdDg, measured by UVvisible spectroscopy at 90 C [7] We used NMR for these studies because it provides a wide range of time-scales for the dynamic analyses Heteronuclear NMR relaxation data, ... L.E., Torchia, D.A & Bax, A (1989) Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease Biochemistry 28, 89728979 ... Saudek, V & Sklenar, V (1992) Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions J Biomol NMR 2, 661665 40 Palmer, 3rd, A.G., Rance, M & Wright, P.E (1991) Intramolecular...
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Báo cáo khoa học: A simple protocol to study blue copper proteins by NMR pot
Ngày tải lên :
08/03/2014, 08:20
... D.J (1980) Natural abundance nitrogen-15 NMR by enhanced heteronuclear spectroscopy Chem Phys Lett 69, 185–188 Ó FEBS 2003 Blue copper proteins studied by NMR (Eur J Biochem 270) 609 76 Kay, L.E., ... proteins by longitudinal NMR relaxation J Am Chem Soc 124, 4093–4096 Ma, L., Philipp, E & Led, J.J (2001) Determination of the electron self-exchange rates of blue copper proteins by super-WEFT NMR spectroscopy ... Diego, CA, USA 61 Vathyam, S., Byrd, R.A & Miller, A.F (2000) Mapping the effects of metal ion reduction and substrate analog binding to Fe-superoxide dismutase by NMR spectroscopy Magn Reson Chem...
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Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
Ngày tải lên :
16/03/2014, 01:20
... in reaction rate enhancement by enzymes J Am Chem Soc 127, 1524815256 Kern D, Eisenmesser EZ & Wolf-Watz M (2005) Enzyme dynamics during catalysis measured by NMR spectroscopy Methods Enzymol ... efciency (kcat Km) by 45% and increased temperature stability (Tm) of the enzyme by C Replacing C67 with alanine resulted in an almost identical drop in activity as that caused by C67S, along with ... of the two W274 variants was not affected by spin-labeling at C67 beyond the small decrease observed in the control Characterization of mobility by EPR spectroscopy Figure shows the EPR spectra...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
Ngày tải lên :
16/03/2014, 14:20
... therefore by directly blocking signal transduction or by interfering with STAT access to the phosphorylated receptor subunits SOCS3, in particular, has a 22-residue N-terminal segment, followed by a ... N-terminal half of the KIR, and 35 residues in the SH2 domain, were shown by NMR to be unstructured This was evinced by the lack of any nonadjacent inter-residue NOEs in those regions, as well ... originally suggested by sequence comparison [3] However, subsequent attempts to determine key residues important for the binding specificity of SOCS3 by structural modelling were hampered by the logical,...
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