... and sea waves are in the stage of potential assessment. Institute of Energy RURAL RENEWABLE ENERGY WEEK IN HANOIEXPERIENCE OFTHE INSTITUTE OF ENERGYINTHE PREPARATION OFTHE RENEWABLE MASTER ... energy, wind energy, solar energy, and geothermal energy. 9 So far, even the high potential of RE resources, their contribution in electricity production in Vietnam is still negligible. 9 The ... negligible. 9 The Government of VN recognized important role of RE in sustainable energy supplying in general and electricity in particular for rural off-grid areas. ắ The electricity Law require...
... localizes inthe cytoplasm of human epithelial cells ofthe gastrointestinal tract andliver, two central organs inthe regulation of ironmetabolism [20,21]. After their synthesis inthe endo-plasmic ... impact in protein integrity, as indicatedby an increase in protein-bound acrolein adducts at the cell surface, which point to acrolein-adducts as areliable marker of lipid peroxidation also in ... third of these cells also expressing CD1d(Fig. 6A, left dot-plot). Interestingly, the increase in phosphatidylserine expression by HepG2 cells grown in iron-rich media was ofthe same order of...
... quantify the amount of recoveredprotein owing to the presence of a high molar excess of BSA remaining inthe buffer. Therefore, a fixed aliquot of the protein was incubated with the indicated ... basic proteins it might be thateither methylation ofthe lysine or specific substitutions in the amino acid sequence, increasing the number of basicresidues of lysine, arginine and histidine, could ... the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.20. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of...
... an increase in binding capacity[25].Identification of nitration site inthe extracellulardomain of ILT2 In the extracellular domain of ILT2, there are severalTyr residues that participate in ... thisis the first report of a post-translational modification of the ILT2 protein. The other member ofthe ILTfamily, LILRA4, has also been found nitrated within the domain Ig-like C2-type 4 inhuman ... band at 42 kDa. The data indicate the intensity of the HLA-G band related to the b-actin band and are representative of three different experiments.ILT2 nitration inthe binding domain A. Dı´az-Lagares...
... participates inthe assembly ofthe mitotic spindle of E. focardii and that both isotypes are involved in the nucleation of other microtubule structures.Finally, we examined the distribution of E. ... isotype.Fig. 1. The macronucleus of Euplotes focardii contains two differ-ent c-tubulin nanochromosomes. (A) Southern blot analysis ofthe two c-tubulin genes of E. focardii using the c-T2 gene ... and HindIII-digested DNA. The sizes (bp) of DNA standards are indicated on the left. The sizes of thetwo c-tubulin nanochromosomes (1600 bp) and their diges-tion products are indicated on the...
... protein bound to the agarose. This binding was con-firmed by the observation that only a trace ofthe pro-tein was left inthe unbound fraction. Pre-incubation of the recombinant protein with ... againstinactivation was observed and the initial GS activitywas maintained (116.5 ± 7.3% ofthe initial activity). The protective effect was abolished when the recom-binant protein was heat-inactivated ... but the bindingwas not affected by preincubation ofthe protein withFP. These results also indicate that Pf1-Cys-Prx canbind to FP at pH 7.4, which is the pH ofthe parasitecytosol. The fact...
... transformation of preginto androstadienol, a precursor inthe biosynthesis of Fig. 5. In uence of increasing concentrations of cyt b5on the relativeformation of androstadienol and DHEA by human P450c17. ... formation of 17a-OH-preg andDHEA. The lack of androstadienol synthesis inthe presence of 1 lM of ketoconazole (C), an inhibitor of cytochromeP450, further demonstrates the specific implication of P450c17 ... with a broadrange of other intracellular signaling pathways [21,22]. The purpose of this research is to examine, at the molecular level, whether thehuman P450c17 overexpressed in HEK-293 cells...
... catalytic site of GalNAc-T1, might beinvolved inthe catalytic function ofthe enzyme.To investigate whether the cysteine residues modified byPCMPS are involved inthe binding of UDP-GalNAc,we ... amajor site of interaction with UDP-GalNAc, Cys214 is alsoinvolved in binding.PCMPS and DFP modification ofthe serine-mutants of GalNAc-T1We also examined the sensitivity ofthe three serine mutantsto ... cysteine residues not involved inthe formation of a disulfide bond), by cysteine-specific labeling, to study the mechanistic involvement ofthe conserved cysteine residues in the function of GalNAc-T1....
... and so the choice ofthe constantlevel of Fru was not critical for determination of DKm(1.23 ± 0.15). The KIE data in Table 4 are instrumen-tal in delineating the kinetic mechanism of AfM1PDHand ... 3, indi-cates that NADH binds to the enzyme prior to binding of Fru6P. The absence of a KIE on kcat⁄ Kmfor the substrate binding first is a clear requirement of the strictly ordered kinetic ... the corresponding binary enzyme–substrate complex. Inspection ofthe KIE data in Table 4 reveals that hydride transfer is partly rate-determining in either direction of each ofthetwo enzy-matic...
... inthe case ofthe K137E mutant of HSP22 (Fig. 4B); however, in this case, the intensity of the band corresponding to the HSP22 dimer wasmore intense than inthe case ofthe wild-type protein.Thus, ... intact protein at the beginning of tryp-sinolysis and at the fixed time of trypsinolysis) against the time of incubation (Fig. 8D). The apparent rateconstants of trypsinolysis under these conditions ... shock proteins, each of which contains the so-called a-crystallin domain consisting of 80–100 residues and located inthe C-terminal part ofthe molecule. The a-crystallin domain consists of six...
... sequences contained in any of their strain members. For the 216-probe set, the minimum number of probes differentiating aDENV-1 strain from the all other strains belonging toone ofthe three other serotypes ... While these values contribute to the fit-ness of a set, the size ofthe set plays a much greater role in an effort to reduce the number of probes needed and thus the cost ofthe array. Therefore, ... present in each individual viralstrain’s genome, (b) present in all 83 dengue strainsregardless of their serotype, (c) unique to each serotype of the virus (present in all strains ofthe serotype),...
... b. The Veins ofthe Head and Neck 1. The Veins ofthe Exterior ofthe Head and Face2. The Veins ofthe Neck3. The Diploic Veins4. The Veins ofthe Brain5. The Sinuses ofthe Dura Mater. ... with the sphenoidal process ofthe palatine and behind this with the ala ofthe vomer. The angular prominence between the posterior margin ofthe vaginal process and the medial border ofthe ... piece ofthe body, during the sixth month; inthe second and third pieces ofthe body, during the seventh month of fetal life; in its fourth piece, during the first year after birth; and in the...