two photon recording of action potentials in mammalian nerve terminals

... applied to the inside of the last cell (A5) of the chain when the APs of all cells were in their plateau phase The intensity and duration of the IS2 pulses were varied over a wide range in order to ... in a retrograde direction down the 5-cell chain B: 1,000 gj-channels inserted (Rgj of 10 MΩ) Again, the rising phase of the APs of the cells were nearly superimposed C: 100 gj-channels (Rgj of ... several VM values and inserted into the GTABLE function Experiments were done with a single chain of cells or cells There were no gap junctions between the cells of the chain under initial conditions...

Ngày tải lên: 13/08/2014, 22:22

... is not possible to infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general value in the mechanism of action of defensins However, our results ... obtained by other groups who also point to the role of the connecting loop of the b-hairpin of defensins A combination of mutational analysis [35] and structural analysis of the plant defensin ... identified two subsites on this molecule comprising Ôresidues in the protruding domain consisting of the type VI b-turn and the first part of b-strand 3Õ (i.e the b-hairpin loop and part of the adjoining...

Ngày tải lên: 20/02/2014, 11:20

... Notes in Mathematic; Springer-Verlag: Berlin, 672, 1978 in Linear Space, [7] L V Thanh, Mean convergence theorems and weak laws of large numbers for double arrays of random variables, Journal of ... sums of Banach- space-valued random elements under some conditions of uniform integrability, of Mathematical Sciences, 138 (1), 2006, 5450-5459 [3] J Hoffmann-Jrgensen and G Pisier, The law of ... the same one in each appearance III Main results Theorem Let r

Ngày tải lên: 23/07/2014, 14:21

... multispecies breakpoint reuse test Technically, findings in [33] (limited breakpoint reuse between different lineages) are not in conflict with findings in [3] (rampant breakpoint reuse in chromosome ... the sites of rearrangements in the human lineage 18 out 162 breakpoints between MRD and QH were used in the human lineage, resulting in 18 = 162 ≈ 11% accurate prediction of human breakpoints, nearly ... 10 of them were used in the human lineage, resulting in 10 = 68 ≈ 16% accurate prediction of human breakpoints, again improving the accuracy of predictions These estimates indicate that TFBM can...

Ngày tải lên: 09/08/2014, 22:23

... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically, ... Figure 8C and 8D (movie of different projection in Additional file 2) shows the results of specimen staining in revealing the detailed localization and shape of the nucleus and of the overall nanoparticle ... micrographs of the EMT and HeLa cells with AuNPs internalized After co-culturing with 500 μM naked AuNPs for 48 h, the endocytotic vesicles inside the cytoplasm of cell lines contained clusters of many...

Ngày tải lên: 11/08/2014, 00:23

... proteins reside or function in the NE remains to be determined In addition to their difference in size and SUN domain locations, these protein subfamilies are distinct in other interesting ways ... plant nuclei contain multiple different SUN-domain proteins Models of the Topology of Plant SUN-domain Proteins The two structural classes of plant SUN-domain proteins found in maize, and shown ... in plants as well [33,63,64] In this regard, we find intriguing that two different laboratories [65,66] recently and independently mapped a recombination control QTL in maize to bin Page 13 of...

Ngày tải lên: 11/08/2014, 11:21

... Attenuation of insulin signaling in cullin-1 mutant does not affect normal dendrite elaboration 86 3.7.5.2 Attenuation of insulin signaling in cullin-1 mutant promotes proximal severing of ... Attenuation of insulin signaling in cullin-1 mutant promotes proximal severing of major dendrites 88 Figure 22: Attenuation of insulin signaling does not rescue dendrite pruning defect in mical ... activating protein HECT homologous to E6 associated protein C-terminus Hh hedgehog IGF insulin like growth factor InR insulin receptor IPB infra-pyramidal bundles xv IPC insulin producing cell IRS insulin...

Ngày tải lên: 09/09/2015, 11:16

... family of signaling proteins Upon agonist binding to β receptors, a signaling cascade is launched involving the heterotrimeric G protein Gq, which activates phospholipase C, increasing inositol ... electrical responses in thin nerve segments Thus, a detailed delineation of the intracardiac sympathetic network structure would aid in better understanding the anatomical underpinnings of functional ... validation of an assay for imaging sympathetic nerves in the living mouse heart, and, second, use of this technique to obtain a three-dimensional wiring diagram of sympathetic nerves in a well-defined...

Ngày tải lên: 24/08/2014, 12:57

... treating single de novo lesions in patients with symptomatic coronary artery disease has been examined in the first four trials of the ongoing ENDEAVOR clinical trials program The results of these ... to maintain activated and clotting time of 250 to 300 s Patients received intracoronary nitroglycerin (0.1 to 0.2 mg) to achieve maximal vasodilatation before undergoing their initial and final ... patients treated in daily practice remains controversial The long-term safety of DESs remains in question (10-11) Despite the results of meta-analyses of randomized studies refuting these concerns...

Ngày tải lên: 25/10/2012, 11:18

... pathogenesis of insulin resistance in humans using magnetic resonance spectroscopy Obesity (Silver Spring) 2006;14 (Suppl 1): 34S–40S 31 Boden G Role of fatty acids in the pathogenesis of insulin resistance ... http://www.medsci.org Int J Med Sci 2010, cellular senescence (40-45) Source of reactive oxygen species in insulin secreting pancreatic β-cells and cells that are targets for insulin action is considered ... leg ulcers contain localized oxidative stress (15) The recent finding revealed that insulin resistance is associated in humans with reduced intracellular antioxidant (11) Interestingly, antioxidants...

Ngày tải lên: 26/10/2012, 09:39

... sustainability financing mechanisms The rapid expansion of intensified agricultural cultivation and urbanization in the MD is strongly affecting wetland forest, resulting ultimately in shrinkage of ... accounting for approximately 57% (17% in the Lower U Minh and 40% in the Upper U Minh) of the total Melaleuca forest area In the U Minh coastal forest areas, the existing peat layers in the soil, ... countries, including Vietnam, became member of the Ramsar Convention The latter being an intergovernmental agreement offers a sound and stable framework for international cooperation in the area of wetland...

Ngày tải lên: 09/12/2013, 22:15

... in inconsistent responses We found inconsistencies in 12.2% of the sample, indicating the difficulties in communicating the information about the mechanism of action To assess the influence of ... would consider using and would stop or continue using In addition, hypothetical choices were examined in terms of a non-specific "method of family planning" and not in terms of actual methods ... cross-sectional survey in a sample of women in reproductive age (18–49) from ten Primary Care Health Centres in various areas of Pamplona, Spain Women attend this kind of centre for obtaining primary care...

Ngày tải lên: 13/02/2014, 06:20

... highlight the situation • maintaining a web-based system of posting treatment failures, including mapping and regularly updating the data on emerging resistant strains • collaborating with partners to ... The development of a pool of resistance genes and the ability of the gonococci to maintain these determinants of resistance within their genetic coding is part of the very nature of N. gonorrhoeae ... and principles for the prevention and care of STIs, to offer guidance to ministry of health officials who have the responsibility of developing and implementing interventions for the control of...

Ngày tải lên: 18/02/2014, 15:20

... NADPÆmin)1Æmg protein)1 Determination of maintenance In order to calculate the amount of glucose used for maintenance, the wild-type strain A26 was used Four 100-mL aliquots of glucose minimal ... addition of acetate to the media containing glucose and propionate, the growth rate of both strains increased and the effect of propionate became less apparent Finally, in media containing acetate ... Determination of maintenance Maintenance is the energy that is used for survival of cells without any biomass formation Determination of maintenance was based on the inhibition of protein biosynthesis...

Ngày tải lên: 19/02/2014, 16:20

... key enzyme in the biosynthesis of polyamines, whose interaction with antizyme, an ODC inhibitory protein, confers to the enzyme a half-life of about h The C-terminal end of ODC contains a PEST ... maintained in the cells by binding to antigen Generation of soluble binding-defective and cysteine-deleted mutants To demonstrate that the observed intracellular colocalization was the result of ... measuring protein mobility and activity in living cells Nature Cell Biol 3, 145–147 Bachmair A, Finley D & Varshavsky AK (1986) In vivo half-life of a protein is a function of its amino-terminal...

Ngày tải lên: 19/02/2014, 18:20

... disruption of the local bonding and steric hindrance by the introduced amino acid Discussion In this paper we have described the protein folding, enzyme function, and thermostability of two novel ... procedure proving very effective in securing a pure protein product The results of kinetic analysis are displayed as Michaelis–Menten plots in Fig 3A The Km, Vmax (as determined by the Direct Linear ... to the purified protein (assuming no major change in the extinction coefficient of the bound cofactor in the case of the mutants) [15] The peak absorbance ratios showed first of all that the wild-type...

Ngày tải lên: 20/02/2014, 02:21

... Penicillin-Streptomycin-Glutamine 1X (Gibco-Life Technology) Cell lines were maintained at 37 C in a humidied incubator containing 10% CO2 mixed with air Cytotoxicity assay Cells were seeded in 24- ... MSSAE in binding buffer (DMEM containing mgặmL)1 BSA and 25 mM Hepes at pH 7.5) At increasing time intervals, cells were washed repeatedly with NaCl/Pi and then treated with 0.6 M NaCl in NaCl/Pi ... growing cells When 125I-labelled MSSAE was incubated in binding buffer in the absence of cells no dimerization occurred (data not shown) These results indicate that a monomeric derivative of BSRNase...

Ngày tải lên: 20/02/2014, 11:20

... living-instrumental activity of daily living (ADLIADL) without help e.g dressing, transferring from bed, toileting or taking bath, preparation of food, eating, shopping and walking Height and weight of the subjects ... Although in bi-variate analysis no gender difference was detected in disability, yet in logistic regression analysis after eliminating the influence of other factors, risk of disability increased in ... problems of the elderly in rural south India Indian Journal of Community Medicine 1990;15: 70-3 Khokhar A, Mehra M Life style and morbidity profile of geriatric population in an urbans community of...

Ngày tải lên: 05/03/2014, 21:20

... Monitoring of VOCs in industrial areas during night times and also extending monitoring in the surrounding villages APPCB will ensure the linkage of Continuous Ambient Air Quality Monitoring Stations ... sweeping: The industry has procured a road sweeping machine with a cost of Rs 0.25 Cr in the year 2004 and cleaning the internal roads d Online monitoring: The industry has provided stack monitoring ... thereby reducing the movement of M/s VPT is in the process of number of trucks and dust fly-off mechanization of stocking, loading and unloading of dusty cargo like coal and iron ore to eliminate the...

Ngày tải lên: 06/03/2014, 16:20

... checkpoint signaling in vivo It may function in the sensing of DNA damage [22] In contrast with the intensive study of RPA in animals and yeast, little is known about this protein in plants In 1997, ... protein–protein interaction assay) (Fig 3C–E) In the two- hybrid assay, the interaction between OsRPA70a and OsRPA32 yielded about 20% of the relative activity of the OsRPA32 and OsRPA14 interaction ... CM (1998) Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA Nucleic Acids Res 26, 5388–5393 17 Stauffer ME & Chazin WJ (2004) Physical interaction between...

Ngày tải lên: 07/03/2014, 21:20