... reference gelof the software-assisted analysis the values of pI predicted for any given length of the strip Both Mr and pI of the spots of interest were then determined by using software-automated ... Solubilization of plant membrane proteins for analysis by two- dimensionalgelelectrophoresis Plant Physiol 1986, 81:802-806 Laemmli UK: Cleavage of structural proteins during the assembly of the head of ... increase in accumulation of the cytosolic isoform of glutamine synthetase (spot 538, GS1-1) was also detected in roots of N plants On the basis of identified peptides by MS analysis it was possible...
... kDa Two- dimensionalgelelectrophoresis and its immunoblot analysisof Neospora caninum tachyzoites 143 Fig Analysisof 2-DE and immunoblot profiles of N caninum tachyzoites (A) A total of 335 ... (KBA-2) mixed with Freunds complete (first Two- dimensionalgelelectrophoresis and its immunoblot analysisof Neospora caninum tachyzoites 141 Fig Comparison oftwo IEF conditions IEF was performed ... by two- Two- dimensionalgelelectrophoresis and its immunoblot analysisof Neospora caninum tachyzoites dimensionalelectrophoresis and mass spectrometry: proteomics of Toxoplasma gondii Int...
... patterns are reproducible from gel to gel The apparent resistance of membrane proteins to electrophoretic analysis is probably the result of incomplete disruption of all protein complexes and aggregate ... (fig 3C) Comgels of membrane proteins to 2-D solubilized by other methods, protein gels solubilized by the latter method exhibited a larger number of polypeptides Similarly, gels of membrane ... by a doctoral fellowship of the Institut National de la Recherche Agronomique and the Région de Lorraine to BH REFERENCES (1976) Two- dimensionalgelelectrophoresisof membrane proteins Biochemistry...
... delivery of CX3CL1 to multiple lung tumors by mesenchymalstemcellsStemCells 2007, 25(7):1618-26 12 Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L: Mesenchymal progenitor cells ... detected by imaging of whole animal (C) imaging of excised lungs (B) and by luciferase expression analysisof lung lysates (D,E) Data are presented as number ofcells per mg of protein in lung ... marrow-derived mesenchymalstemcells in the treatment of gliomas Cancer Res 2005, 65(8):3307-18 Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M: Bone marrow-derived mesenchymalstem cells...
... accelerated degeneration of the treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow-derived mesenchymalstemcells (MSCs) The use of these cells would allow ... cell-mediated mesenchymalstem cell differentiation StemCells 2006, 24:707-716 27 Richardson SM, Hughes N, Hunt JA, Freemont AJ, Hoyland JA: Human mesenchymalstem cell differentiation to NP-like cells ... E, Bertram H, Abel R, Chen B, Winter A, Richter W: Induction of intervertebral disc-like cells from adult mesenchymalstemcellsStemCells 2005, 23:403-411 44 Risbud MV, Albert TJ, Guttapalli...
... marrow-derived mesenchymalstemcells and neural progenitor cells Haematologica 2003, 88:126-133 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation ofmesenchymal ... Human mesenchymalstemcells modulate allogeneic immune cell responses Blood 2005, 105:1815-1822 29 Rasmusson I, Ringden O, Sundberg B, Le Blanc K: Mesenchymalstemcells inhibit the formation of ... L-selectin)/lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts Augments B responses/ plasma cells Growth, differentiation, and isotype-switching of B cells/ B cells Ligand...
... Expression levels of ADAM and adhesion molecules during chondrogenic differentiation ofmesenchymalstemcells (MSCs) The gene-expression (MSCs) profile ofmesenchymalstemcells (MSCs; n = 2) ... the analysisof the data PL-P helped in the analysisof the data FC procured the samples for the isolation of MSCs PC participated in the analysisof the data CJ participated in the design of ... 39 40 41 42 43 44 45 46 47 48 49 characterization ofmesenchymalstemcellsStemCells 2006, 24:475-481 Charo IF, Ransohoff RM: The many roles of chemokines and chemokine receptors in inflammation...
... Regulation of stemness and stem cell niche ofmesenchymalstem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymalstemcells ... human embryonic bone marrow mesenchymalstemcells Oncol Rep 2004, 12:501-508 22 Lee HJ, Choi BH, Min BH, Park SR: Changes in surface markers of human mesenchymalstemcells during the chondrogenic ... characteristics of hepatocyte growth factor-modified murine mesenchymalstemcells In Vivo 2009, 23:21-27 Zhi-Gang Z, Wei-Ming L, Zhi-Chao C, Yong Y, Ping Z: Immunosuppressive properties ofmesenchymal stem...
... ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number ofstem cell passages and site of injection; all can affect the outcome of MSCs ... migration ofmesenchymalstemcells to conditioned medium from tumor cells or bone marrow cellsStemCells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stemcells ... C: (×400) Area of cell drop out with stem cells, D: (×400) No nodularity & liver appears normal, few collections of round to oval stemcells in lobules Page of 11 Figure Detection of MSCs labeled...
... http://genomemedicine.com/content/3/10/68 Page of 12 Table Dysregulation of transcriptional networks in stemcells and disease Gene/protein Role in ESCs Role in disease c-MYC Involved in the expression of self-renewal genes ... may offer an experimental means of verifying the relationship of SNPs or haplotypes with diseases Abbreviations CHARGE, Coloboma of the eye, Heart defects, Atresia of the choanae, Retardation of ... human embryonic stemcells Cell 2005, 122:947-956 Cole MF, Johnstone SE, Newman JJ, Kagey MH, Young RA: Tcf3 is an integral component of the core regulatory circuitry of embryonic stemcells Genes...
... et al.: The in vitro migration capacity of human bone marrow mesenchymalstem cells: comparison of chemokine and growth factor chemotactic activities StemCells 2007, 25:1737-1745 24 Segers VF, ... transmigration and invasion ofmesenchymalstemcells J Mol Cell Cardiol 2008, 44:1072-1084 30 Estrada R, Li N, Sarojini H, An J, Lee MJ, Wang E: Secretome from mesenchymalstemcells induces angiogenesis ... (right) Serum of control rats was placed in the lower transwell compartment in both cases Bottom: Normalized indices for the migration ofmesenchymalstemcells induced by the serum of control and...
... Characterisation of canine adipose tissue derived mesenchymalstemcells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymalstemcells (MSCs) ... effects of curcumin inhibit the induction of degradative and inflammatory pathways by IL-1β through revoking activation of caspase-3, production of COX-2, phosphorylation of Iκ-Bα and inhibition of ... the biofunctions and biotransformations of curcumin Biofactors 2000, 13:153-158 19 Conget PA, Minguell JJ: Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells...
... Figure Mesenchymalstemcells used during this study Mesenchymalstemcells used during this study Typical morphological aspects ofmesenchymalstemcells observed through culture flask (A) Mesenchymal ... distribution ofmesenchymalstemcells in vivo Figure Early dynamic distribution ofmesenchymalstemcells in vivo Sequential whole body scintigraphies after infusion of indium-111 labeled mesenchymalstem ... perspective of cellular therapy by intravenous stemcells infusion The participation ofstemcells in several vascular diseases pathogenesis was first proved with haematopoietic stemcells (HSCs)...
... interventions28 MesenchymalStemCells In The Treatment Of Acute Myocardial Infarction With the emergence ofstemcells as potential regenerative medicine, attempts to use stemcells to reduce ... 2004 Mesenchymalstemcells and their potential as cardiac therapeutics Circ Res 95, 9–20 Quesenberry, P.J., Aliotta, J.M., 2008 The paradoxical dynamism of marrow stem cells: considerations ofstem ... CHARACTERIZATION OF THE SECRETION OFMESENCHYMALSTEMCELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI (B.Eng (Hons.)), NTU A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS...
... TRANSPLANTATION OFMESENCHYMALSTEMCELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... Suppression of complement system activation……………… 106 xii 3.2.5.2 Suppression of NK cell activation and recruitment at the SNc 108 Immune potential analysisofmesenchymalstemcells ………………… ... vitro analysisof immune potential ofmesenchymalstemcells ………… 133 Systemic MSC transplantation rescue the dopaminergic neurons from MPTP toxicity………………………………………………………………………… 134 5.1 Rescue of...
... Graft-Versus-Host Disease HaMSC Human Adult MesenchymalStemCells HfMSC Human Fetal MesenchymalStemCells HSC Hematopoietic stemcells HUVEC Human Umbilical Vein Endothelial Cells ICAM-1 Inter-cellular cell ... Introduction 1.1 MesenchymalstemcellsMesenchymalstemcells (MSC), otherwise known as bone marrow stromal cells, was discovered by Friedenstein who noticed that transplantation of bone marrow cells resulted ... full of love i Table of Contents Introduction .1 1.1 Mesenchymalstemcells 1.1.1 MSC of fetal origin 1.1.2 Potential applications of MSC 1.1.3 Mode of...
... x List of Abbreviations and acronyms xii Introduction 1.1 Mesenchymalstemcells (MSC) 1.1.1 Definition ofstemcells 1.1.2 Criteria of being stemcells ... microlitres xvii INTRODUCTION 1 Introduction 1.1 Mesenchymalstemcells (MSC) 1.1.1 Definition ofstemcellsStemcells (SC) are defined functionally as cells that have the capacity to self-renew and ... forms of plasticity (the ability of adult stemcells to acquire mature phenotypes that are different from their tissue of origin) (Grove et al., 2004) Examples include MSC giving rise to cells of...
... Isolation ofmesenchymalstemcellsof fetal or maternal origin from human placenta StemCells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation ofmesenchymal ... clustering analysisof AT-MSC-Hepa Unsupervised hierarchical cluster analysis was performed by sorting of 1639 altered genes (Fig 3A) This analysisof microarray data revealed a striking similarity of ... clearly demonstrated the utility of microarray analysis in proving the hepatic differentiation of AT-MSCs Moreover, analysisof GO groups indicated that many of the 1639 up- or down-regulated...
... dimension gels SDS-PAGE was performed at constant effect (10 W /gel) for about h and 30 using the Ettan DALT II system (Amersham Biosciences) Staining of gels and gel image analysis Second dimension gels ... fluid Of the spots picked from the 2D-gels and submitted to MALDI-TOF analysis 61 spots were identified (figure and table 1) 21 of these were identified as separate proteins The majority of the ... http://respiratory-research.com/content/6/1/118 Figure Expression analysisof psoriasin with western blot Expression analysisof psoriasin with western blot A: Western blot analysisof NLF from three healthy non-allergic...