two dimensional gel electrophoresis analysis of mesenchymal stem cells

... reference gel of the software-assisted analysis the values of pI predicted for any given length of the strip Both Mr and pI of the spots of interest were then determined by using software-automated ... Solubilization of plant membrane proteins for analysis by two- dimensional gel electrophoresis Plant Physiol 1986, 81:802-806 Laemmli UK: Cleavage of structural proteins during the assembly of the head of ... increase in accumulation of the cytosolic isoform of glutamine synthetase (spot 538, GS1-1) was also detected in roots of N plants On the basis of identified peptides by MS analysis it was possible...

Ngày tải lên: 12/08/2014, 03:21

... kDa Two- dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites 143 Fig Analysis of 2-DE and immunoblot profiles of N caninum tachyzoites (A) A total of 335 ... (KBA-2) mixed with Freunds complete (first Two- dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites 141 Fig Comparison of two IEF conditions IEF was performed ... by two- Two- dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites dimensional electrophoresis and mass spectrometry: proteomics of Toxoplasma gondii Int...

Ngày tải lên: 07/08/2014, 17:23

... patterns are reproducible from gel to gel The apparent resistance of membrane proteins to electrophoretic analysis is probably the result of incomplete disruption of all protein complexes and aggregate ... (fig 3C) Comgels of membrane proteins to 2-D solubilized by other methods, protein gels solubilized by the latter method exhibited a larger number of polypeptides Similarly, gels of membrane ... by a doctoral fellowship of the Institut National de la Recherche Agronomique and the Région de Lorraine to BH REFERENCES (1976) Two- dimensional gel electrophoresis of membrane proteins Biochemistry...

Ngày tải lên: 08/08/2014, 23:22

... delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells Stem Cells 2007, 25(7):1618-26 12 Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L: Mesenchymal progenitor cells ... detected by imaging of whole animal (C) imaging of excised lungs (B) and by luciferase expression analysis of lung lysates (D,E) Data are presented as number of cells per mg of protein in lung ... marrow-derived mesenchymal stem cells in the treatment of gliomas Cancer Res 2005, 65(8):3307-18 Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M: Bone marrow-derived mesenchymal stem cells...

Ngày tải lên: 20/06/2014, 07:20

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... oT syad 41 rof )ASU ,negortivnI ;FGNh( rotcaf htworg laruen namuh lm/gn 01 dna )dnalreztiwS ,ehcoR ;FGEh( rotcaf htworg lamredipe namuh lm/gn 01 ,)dnalreztiwS ,ehcoR ;FGFb( rotcaf htworg tsalborbif...

Ngày tải lên: 07/08/2014, 18:21

... accelerated degeneration of the treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow ... cell-mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 27 Richardson SM, Hughes N, Hunt JA, Freemont AJ, Hoyland JA: Human mesenchymal stem cell differentiation to NP-like cells ... E, Bertram H, Abel R, Chen B, Winter A, Richter W: Induction of intervertebral disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 44 Risbud MV, Albert TJ, Guttapalli...

Ngày tải lên: 09/08/2014, 01:22

... marrow-derived mesenchymal stem cells and neural progenitor cells Haematologica 2003, 88:126-133 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal ... Human mesenchymal stem cells modulate allogeneic immune cell responses Blood 2005, 105:1815-1822 29 Rasmusson I, Ringden O, Sundberg B, Le Blanc K: Mesenchymal stem cells inhibit the formation of ... L-selectin)/lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts Augments B responses/ plasma cells Growth, differentiation, and isotype-switching of B cells/ B cells Ligand...

Ngày tải lên: 09/08/2014, 08:23

... Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) ... the analysis of the data PL-P helped in the analysis of the data FC procured the samples for the isolation of MSCs PC participated in the analysis of the data CJ participated in the design of ... 39 40 41 42 43 44 45 46 47 48 49 characterization of mesenchymal stem cells Stem Cells 2006, 24:475-481 Charo IF, Ransohoff RM: The many roles of chemokines and chemokine receptors in inflammation...

Ngày tải lên: 09/08/2014, 10:20

... Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... human embryonic bone marrow mesenchymal stem cells Oncol Rep 2004, 12:501-508 22 Lee HJ, Choi BH, Min BH, Park SR: Changes in surface markers of human mesenchymal stem cells during the chondrogenic ... characteristics of hepatocyte growth factor-modified murine mesenchymal stem cells In Vivo 2009, 23:21-27 Zhi-Gang Z, Wei-Ming L, Zhi-Chao C, Yong Y, Ping Z: Immunosuppressive properties of mesenchymal stem...

Ngày tải lên: 10/08/2014, 10:20

... ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs ... migration of mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells Stem Cells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stem cells ... C: (×400) Area of cell drop out with stem cells, D: (×400) No nodularity & liver appears normal, few collections of round to oval stem cells in lobules Page of 11 Figure Detection of MSCs labeled...

Ngày tải lên: 10/08/2014, 10:21

... http://genomemedicine.com/content/3/10/68 Page of 12 Table Dysregulation of transcriptional networks in stem cells and disease Gene/protein Role in ESCs Role in disease c-MYC Involved in the expression of self-renewal genes ... may offer an experimental means of verifying the relationship of SNPs or haplotypes with diseases Abbreviations CHARGE, Coloboma of the eye, Heart defects, Atresia of the choanae, Retardation of ... human embryonic stem cells Cell 2005, 122:947-956 Cole MF, Johnstone SE, Newman JJ, Kagey MH, Young RA: Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells Genes...

Ngày tải lên: 11/08/2014, 12:21

... et al.: The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities Stem Cells 2007, 25:1737-1745 24 Segers VF, ... transmigration and invasion of mesenchymal stem cells J Mol Cell Cardiol 2008, 44:1072-1084 30 Estrada R, Li N, Sarojini H, An J, Lee MJ, Wang E: Secretome from mesenchymal stem cells induces angiogenesis ... (right) Serum of control rats was placed in the lower transwell compartment in both cases Bottom: Normalized indices for the migration of mesenchymal stem cells induced by the serum of control and...

Ngày tải lên: 12/08/2014, 11:22

... Characterisation of canine adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymal stem cells (MSCs) ... effects of curcumin inhibit the induction of degradative and inflammatory pathways by IL-1β through revoking activation of caspase-3, production of COX-2, phosphorylation of Iκ-Bα and inhibition of ... the biofunctions and biotransformations of curcumin Biofactors 2000, 13:153-158 19 Conget PA, Minguell JJ: Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells...

Ngày tải lên: 12/08/2014, 14:22

... Figure Mesenchymal stem cells used during this study Mesenchymal stem cells used during this study Typical morphological aspects of mesenchymal stem cells observed through culture flask (A) Mesenchymal ... distribution of mesenchymal stem cells in vivo Figure Early dynamic distribution of mesenchymal stem cells in vivo Sequential whole body scintigraphies after infusion of indium-111 labeled mesenchymal stem ... perspective of cellular therapy by intravenous stem cells infusion The participation of stem cells in several vascular diseases pathogenesis was first proved with haematopoietic stem cells (HSCs)...

Ngày tải lên: 12/08/2014, 18:20

... interventions28 Mesenchymal Stem Cells In The Treatment Of Acute Myocardial Infarction With the emergence of stem cells as potential regenerative medicine, attempts to use stem cells to reduce ... 2004 Mesenchymal stem cells and their potential as cardiac therapeutics Circ Res 95, 9–20 Quesenberry, P.J., Aliotta, J.M., 2008 The paradoxical dynamism of marrow stem cells: considerations of stem ... CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL STEM CELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI (B.Eng (Hons.)), NTU A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS...

Ngày tải lên: 10/09/2015, 08:42

... TRANSPLANTATION OF MESENCHYMAL STEM CELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... Suppression of complement system activation……………… 106 xii  3.2.5.2 Suppression of NK cell activation and recruitment at the SNc 108 Immune potential analysis of mesenchymal stem cells ………………… ... vitro analysis of immune potential of mesenchymal stem cells ………… 133 Systemic MSC transplantation rescue the dopaminergic neurons from MPTP toxicity………………………………………………………………………… 134 5.1 Rescue of...

Ngày tải lên: 11/09/2015, 09:05

... Graft-Versus-Host Disease HaMSC Human Adult Mesenchymal Stem Cells HfMSC Human Fetal Mesenchymal Stem Cells HSC Hematopoietic stem cells HUVEC Human Umbilical Vein Endothelial Cells ICAM-1 Inter-cellular cell ... Introduction 1.1 Mesenchymal stem cells Mesenchymal stem cells (MSC), otherwise known as bone marrow stromal cells, was discovered by Friedenstein who noticed that transplantation of bone marrow cells resulted ... full of love i Table of Contents Introduction .1 1.1 Mesenchymal stem cells 1.1.1 MSC of fetal origin 1.1.2 Potential applications of MSC 1.1.3 Mode of...

Ngày tải lên: 13/10/2015, 15:54

... x List of Abbreviations and acronyms xii Introduction 1.1 Mesenchymal stem cells (MSC) 1.1.1 Definition of stem cells 1.1.2 Criteria of being stem cells ... microlitres xvii INTRODUCTION 1 Introduction 1.1 Mesenchymal stem cells (MSC) 1.1.1 Definition of stem cells Stem cells (SC) are defined functionally as cells that have the capacity to self-renew and ... forms of plasticity (the ability of adult stem cells to acquire mature phenotypes that are different from their tissue of origin) (Grove et al., 2004) Examples include MSC giving rise to cells of...

Ngày tải lên: 16/10/2015, 15:35

... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal ... clustering analysis of AT-MSC-Hepa Unsupervised hierarchical cluster analysis was performed by sorting of 1639 altered genes (Fig 3A) This analysis of microarray data revealed a striking similarity of ... clearly demonstrated the utility of microarray analysis in proving the hepatic differentiation of AT-MSCs Moreover, analysis of GO groups indicated that many of the 1639 up- or down-regulated...

Ngày tải lên: 30/03/2014, 04:20

... dimension gels SDS-PAGE was performed at constant effect (10 W /gel) for about h and 30 using the Ettan DALT II system (Amersham Biosciences) Staining of gels and gel image analysis Second dimension gels ... fluid Of the spots picked from the 2D-gels and submitted to MALDI-TOF analysis 61 spots were identified (figure and table 1) 21 of these were identified as separate proteins The majority of the ... http://respiratory-research.com/content/6/1/118 Figure Expression analysis of psoriasin with western blot Expression analysis of psoriasin with western blot A: Western blot analysis of NLF from three healthy non-allergic...

Ngày tải lên: 12/08/2014, 18:22