tumor antigens and immune regulation in cancer immunotherapy

... (BMS-354825) tyrosine kinase inhibitor suppresses invasion and induces cell cycle arrest and apoptosis of head and neck squamous cell carcinoma and non-small cell lung cancer cells Clin Cancer Res 11, ... little clinical benefit in the treatment of solid tumours and are now being tested in combination therapies [47] Tyrosine kinase inhibitors are widely and successfully used in the treatment of cancer, ... revealed that DAPK protein was inactive, suggesting that the kinase activity of DAPK was inactivated in cancer cells [38] Our own studies assessing protein expression of DAPK in freshly isolated...

Ngày tải lên: 29/03/2014, 08:20

... liver and pancreas by immunohistochemical staining In contrast, PSA was stained in both prostate cancer tissue and non-cancerous tissue (Figure 2C) These data indicated that AMACR had a mostly cancer- specific ... Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Surgically resected prostate cancer ... suspended with mL of PBS containing 1% formaldehyde, and analyzed by FACScan (Becton Dickinson, Mountain View, CA) Binding affinity was evaluated by comparing mean fluorescence intensity (MFI) of HLA-...

Ngày tải lên: 18/06/2014, 15:20

... becoming one of the most significant issues in treatment decision-making, in general, and more so in prostate cancer [1] Thus late rectal and urinary damage became a major concern in prostate cancer; ... results, and evaluates the relationships between pretreatment, acute and late rectal and urinary syndromes and tumor- , patient- and treatment-related factors In the last years of the study, we investigated ... Takeshima et al have found that tumor- specific CTL, which were induced in the draining lymph nodes and tumor tissue of mice by RT, are fundamental to the inhibition of cancer growth [37] The immunological...

Ngày tải lên: 09/08/2014, 09:20

... lymphomagenesis in mice [44] The miRNA miR-21 is overexpressed in many solid tumors, including breast, colon, lung, prostate and stomach, and in endocrine pancreas tumors, glioblastomas and uterine leiomyomas ... breast cancer cells in vitro and in xenografts in mice [48] miRNAs as biomarkers in cancer Their fundamental role in development and differentiation, and their pervasive corruption in lineagedependent ... tissues in various cancer types, including CLL, breast cancer, glioblastoma, thyroid papillary carcinoma, hepatocellular carcinoma, lung cancer, colon cancer and endocrine pancreatic tumors [8,17–22,26,45,52–54]...

Ngày tải lên: 16/03/2014, 01:20

... Wnt/βcatenin and cadherin pathway genes including N-cadherin and P-cadherin but low E-cadherin protein expression This finding demonstrated the highgrade serous ovarian cancer contained messenchymal ... colorectal cancer, which will be discussed in details below a Chromosomal instability (CIN) Chromosomal instability lead to increase rate of losing or gaining chromosomes during cell division and accounts ... in tumor These include MMP1, MMP3, MMP14 and MMP7 Other cell-cell adhesion-related molecules for examples laminins (LAMA4, LAMA5, LAMB1, LAMB2 and LAMC2) and integrins (ITGA5, ITGB5, ITGA11 and...

Ngày tải lên: 30/03/2014, 09:20

... K Rathinasamy and D Panda [10] The binding site of benomyl in tubulin is yet to be determined However, it has been suggested that benomyl binds to tubulin at a site that is distinct from ... the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is required for inactivating the spindle checkpoint signal and progression in the cell cycle [20] The ... beneficial in chemotherapy Similar to taxol and vinblastine, benomyl also induces apoptosis in HeLa cells, indicating that benomyl may be useful in combination with other potent anticancer drugs...

Ngày tải lên: 30/03/2014, 10:20

... propagates inflammatory responses and is a significant RAGE ligand in the setting of sepsis and acute inflammation HMGB1 is an apparent autocrine/paracrine regulator of monocyte invasion, involving ... upregulation of inflammation in this setting [181] Another RAGE ligand, AGE-CML, is present in endoneurial and epineurial mononuclear cells in chronic inflammatory demyelinating polyneuropathy and vasculitic ... RAGE and its ligands play essential roles in inflammation, neurobiology, cancer, and numerous other conditions Each ligand distinctly activates RAGE and contributes to the innate and adaptive immune...

Ngày tải lên: 18/06/2014, 15:20

... understanding of this repeating immune cycle might be able to assist the clinician by pinpointing recurring opportunities to selectively enhance Teffectors and/ or deplete or inhibit T-reg cells, in ... cancer and independently associated with the increased risk of colorectal cancer in men [14], and overall cancer risk [15] Interleukin-6 (IL-6) has been used for the diagnosis of colorectal cancer ... Diurnal, Seasonal, and Blood-Processing Patterns in Levels of Circulating Fibrinogen, Fibrin D-Dimer, C-Reactive Protein, Tissue Plasminogen Activator, and von Willebrand Factor in a 45-YearOld...

Ngày tải lên: 18/06/2014, 15:20

Ngày tải lên: 18/06/2014, 19:20

... chain, IL-2Rg) which may be important in signaling and in determining how the cytokine influences the innate or adaptive immune response and/ or the interaction of these responses Dr Margolin ... and products, including arginase, iNOS and TGFb Arginase reduces arginine, an amino acid required for T cell function and signaling through the TCR Thus by depleting arginine, this enzyme arrests ... consistent sample handling, processing, testing and reporting, can help eliminate assay variation in clinical trials While this approach introduces a delay in testing due to shipping of samples to...

Ngày tải lên: 18/06/2014, 19:20

... European Organization for Research and Treatment of Cancer QLQ-C30: a quality-of-life instrument for use in international clinical trials in oncology J Natl Cancer Inst 1993, 85(5):365-376 de Haes ... close to in previous studies: 6–7 in hepatobiliary carcinoma [13], and 5–6 in breast cancer [10] Final results were summarized as a range of MID esti- http://www.hqlo.com/content/5/1/70 mates and ... Drs Pickard and Cella have received consulting fees from GlaxoSmithKline, which financed this manuscript including the articleprocessing charge They not have any stocks or shares in an organization...

Ngày tải lên: 18/06/2014, 22:20

... considered as indications for adjuvant therapy Histological processing was obtained with formalin fixation followed by paraffin inclusion, μm sectioning, and staining with hematoxylin and eosin Morphologic ... 50-70% in the stomach, 25-30% in the small intestine, 5-10% in the colon-rectum, < 5% in the esophagus, the remaining may arise within the omentum or within the peritonel layers (Extra-Gastro-intestinal ... Meis-Kindblom JM, Odén A, Dortok A, Gustavsson B, Sablinska K, Kindblom LG: Gastrointestinal stromal tumors: the incidence, prevalence, clinical course, and prognostication in the preimatinib...

Ngày tải lên: 09/08/2014, 01:24

... suggesting that the elevation of IL-4 production during an ongoing immune response to tumor can down-regulate Th1 cytokine production Therefore, in gastrointestinal cancer patients, the initial tumor ... adding an equal volume of 4% formaldehyde fixative After washing and incubating with PBS/BSA/saponin for 10 min, cells were incubated with for 30 min, respectively Results were analyzed using ... Th cells to certain antigens and cytokines causes CD4+ T cells to assume one of these two distinct phenotypes Th1 cells produce predominantly interferon-γ (IFN-γ) and interleukin-2 (IL-2), whereas...

Ngày tải lên: 09/08/2014, 04:21

... assays, and was involved in data analysis CL was involved in programming tasks (PERL/R) and JB in database management (Protecs) KO did all gene expression analysis experiments and provided infection ... (reactions in Tables S2 and S3 in Additional file 1) of metabolism in staphylococci systematically included all pathways for which gene expression data pointed to major changes (Tables and 2) in individual ... baculovirus-infected insect cells were incubated with the corresponding substrates and IQ-143 at concentrations of μM, 10 μM, and 100 μM as described by Unger and Frank [34] As shown here (and in Figure...

Ngày tải lên: 09/08/2014, 22:24

... infections, and death We believe that, although these findings need to be confirmed in a larger multicentered clinical study, our results are in line with the recent commentary of Hotchkiss and ... anti-PD-1 blocking antibodies in septic patients given that these molecules are already being tested (and well tolerated) in clinical trials in patients with cancer Although this hypothesis remains a ... DHOS-Inserm ‘Recherche Clinique Translationnelle 2009’ (to GM and FG), by Fondation Innovation en Infectiologie (FINOVI) (to GM and FV), by the French Ministry of Health (PHRC 2008) (to GM and...

Ngày tải lên: 14/08/2014, 08:21

... 2.3.3.2 Determination of oxidative markers in liver 48 2.3.4 Cryo-sectioning 50 2.3.5 Hematoxylin and Eosin staining 51 2.3.6 Oil red staining 52 2.3.7 Sirius red staining 52 2.3.8 Ferrozine assay ... et al., 2008) Besides participating in synthesis of hemoglobin and other iron-containing proteins, the excess iron is stored in a protein called ferritin to minimize its toxicity yet keep it ... immunoassays using anti-DNPH antibodies, or by isolation using biotin-binding columns following a reaction with biotin hydrazide and identified using fluorescently labeled avidin (Chevion et...

Ngày tải lên: 09/09/2015, 10:18

... for independent production companies in China They are representative of Chinese private producers Interviewing them can help in understanding the situation of independent producers in China and ... producers in China’s market Hence, obtaining interviews with people in these two branches can help with understanding how they respond to restrictive media policies and censorship in China and how ... Changsha and Wuhan (information of “Interviewee and Interview Objective” is provided in Table 1-1) During my six months of fieldwork, I spent three and half months conducting fieldwork in the CPD,...

Ngày tải lên: 11/09/2015, 10:04

... patterning and differentiation in Arabidopsis thaliana through binding assay and expression analysis The GIK protein contains an AThook DNA binding motif that is widely found in chromosomal proteins ... expression in flowers…………… ………65 3.2.8 GIK is a bona fide matrix protein and binds to ETT putative MARs in vitro and in vivo………………… ……………………………………69 3.2.9 MAR is essential for GIK-mediated ETT downregulation…………… ... was initiated based on a bioinformatics screen looking for direct binding targets of AG In the first part of my study, I demonstrate that GIANT KILLER (GIK) is a direct target of AG in the regulation...

Ngày tải lên: 14/09/2015, 08:46

... protein binding site for MADS -box proteins GIK encodes a protein with 285 amino acids containing an AT-hook DNA binding motif and an uncharacterized plant -and- prokaryote conserved domain (Figure ... following AG induction in developing flowers The transgenic line contains a gene coding for a fusion of the AG protein and the steroid binding domain of the rat glucocorticoid receptor (AG-GR) in ... membrane was stained with Coomassie Blue to show protein loading 2.14 South-western blotting (in vitro MAR binding assay) Recombinant proteins GIK-AT (amino acids 74-173) and GIK-MUT (amino acids 74-173;...

Ngày tải lên: 14/09/2015, 08:46