ttc0096 by nterminal amino acid sequence analysis and malditofms

... Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis Richard Griessler1,2,3, Alexandra Schwarz1,3, Jan Mucha2 and Bernd Nidetzky1,3 Institute of Food Technology and 2Centre ... Comparison of the StP amino acid sequence with the sequences of EcMalP and rmGP The alignment was performed with the MEGALIGN program using CLUSTALW with standard settings Amino acids conserved are ... alignment of the amino acid sequences of StP, rmGP and maltodextrin phosphorylase from Escherichia coli (EcMalP) is shown in Fig rmGP and EcMalP were chosen for structure-based sequence comparison

Ngày tải lên: 31/03/2014, 01:20

... compare to 3909 of ATCC 13047 and 3564 of strain 638 MR2 codes highest for the metabolism of carbohydrates and amino acids followed by membrane transport mechanism and production of cofactor, vitamins, ... DNA Metabolism Fatty Acids, Lipids, and Isoprenoids Nitrogen Metabolism Dormancy and Sporulation Respiration Stress Response Metabolism of Aromatic Compounds Amino Acids and Derivatives Sulfur ... N and Bhatnagar, A., Lindane, chlorpyriphos and quinalphos residues in mustard seed and oil Bulletin of Environmental Contamination and Toxicology 67, 122-25 (2001) Horne, I., Sutherland, T and

Ngày tải lên: 14/01/2020, 14:45

... subtelomeric repeats and centromeric repeats in radish In addition to 5S rDNA and 45S rDNA, three tandemly organized repeats (CL1, CL25 and CL43) were identified by bioinformatics analysis of the ... both extended tandem repeats and short non-tandem-repetitive sequences Genome Res 2010;20:1219–28 46 Lü N, Yamane K, Ohnishi O Genetic diversity of cultivated and wild radish and phylogenetic ... chromosomes 1, 4, 5, 6, 7, 8, and 9, and both arms of chromosome with one pairs of signals Signals were large and strong on chromosomes and 7, but weak on chromosomes 1, 3, 4, 6, and Two pairs of 5S rDNA

Ngày tải lên: 26/05/2020, 20:50

... network to orchestrate the chemistry of life Phylogenetic analysis of nucleic acid and protein sequences is an important area of sequence analysis Phylogenetic relationships among the genes can ... regions and high-throughput technologies like Chip-seq experiments JASPAR is a web interface for browsing, searching and subset collection, online sequence analysis and tools for genome-wide and ... JASPAR database The 11 sequences were used for generation of frequency matrix and sequence logo of binding site sequence for CREB1 shown in Fig 2.1 All the binding site sequences were represented

Ngày tải lên: 14/10/2020, 18:28

... three-dimensional structures of a-amino acids and those of b-amino acids and posed the following questions Which structures of b-amino acids overlap the canonical structures of a-amino acids? Subsequently, ... [18] The wide use of b-amino acids is prevented by the small number of enantiomerically pure b2-amino acids commercially available and the cost of synthesizing b3-amino acids Therefore, the design ... and w and were subsequently refined by varying /, w angles in intervals of 10° and setting h angle to ) 60 ± 10° and 60 ± 10° Each /, h, and w-value was fixed by applying a harmonic potential and

Ngày tải lên: 08/03/2014, 08:20

... charged amino acids is about 26% and the calculated frequency of charge-deter- mined amino acid-amino acid interactions is 5 × 5/2 of 20 × 20/2, i.e. only 6.25%. The influence of charge on amino acid ... HM(A) and HM(B) are the hydrophobic moments of the amino acids A and B and HM(Arg)-HM (Ile) = 10.6. This formula gives the maximal index (20) for identical amino acids (closest hydrophobicity) and ... charge; Asp and Glu are negatively charged, acidic (pI 2.7 and 3.2) and His, Lys, Arg are positively charged, basic (pI 7.5, 9.7, and 10.7). Only 16/64 codons encode charged amino acids, so the

Ngày tải lên: 13/08/2014, 23:20

... characterized entCPS and KS from A thaliana Identical amino acids are marked by black boxes and amino acids with similar side chains are marked by gray boxes Conserved motifs are labeled and the highly ... kaurene synthase of Arabidopsis thaliana One amino acid determines the product specificity of PtTPS19 and PtTPS20 Although the PtTPS19 and PtTPS20 amino acid sequences were highly similar (99.1 %), ... RESEARCH ARTICLE Open Access One amino acid makes the difference: the formation of ent-kaurene and 16α-hydroxyent-kaurane by diterpene synthases in poplar Sandra Irmisch*, Andrea T Müller, Lydia Schmidt,

Ngày tải lên: 26/05/2020, 20:12

... varying conditions Materials and methods Retrieval and analysis of psbO sequences Sequences of expressed psbO genes were retrieved as ESTs (expressed sequence tags) and assembled ESTs (PUTs, PlantGDB-assembled ... other, giving the values 0, 0.5 or (for amino acids in both isoforms identical, amino acid in one isoform identical or no identical amino acid) for each position and each comparison Values for each ... retrieved sequences were aligned using MAFFT v7.017 [31] and incomplete and unreliable sequences were excluded from further analyses (see analysed sequences in Additional file 1) Spinach psbO sequence

Ngày tải lên: 26/05/2020, 21:20

... experiments RR and NF performed oocyte uptake experiments and analysis AP and JRK performed Seahorse experiments and analysis NT contributed to study design and Seahorse experiment analysis JH conceived ... breast cancer cell growth and viability through disruption of intracellular amino acid homeostasis and inhibition of downstream metabolic and growth pathways Keywords: Amino acids, Metabolism, Triple-negative, ... mediated in part by direct inhibition of multiple amino acid transporters, and not just by inhibition of LAT1 and ASCT2 Although not normally expressed at high levels in breast tissue, we and others

Ngày tải lên: 24/07/2020, 01:45

... changes in amino acid content, 16 amino acids in leaves from the different treatment groups were detected (Fig 10a and b) Based on the general trend, the levels of most of the amino acids increased ... accumulation of free amino acids is crucial in enhancing tolerance to salt stress, but the amino acids are not merely osmotic adjustment substances As an important amino acid to connect carbon and nitrogen ... to leaves, increasing amino acid content and strengthening antioxidant metabolism Endogenous GABA content was induced by salt and exogenous GABA at both the transcriptional and metabolic levels

Ngày tải lên: 28/06/2021, 20:42

... experiment into 20 amino acids and five elements (C, H, N, O and S), with protein abundances converted to relative abundances of amino acids and elements The method is robust and very sensitive; ... Proteome-wide Amino aCid and Elemental composition (PACE) analysis is a novel and informative way of interrogating the proteome The PACE approach consists of in silico decomposition of proteins detected and ... Academy of Sciences and Genetics Society of China Production and hosting by Elsevier Modern proteomics analysis provides the identities and the relative abundance changes for thousands of proteins

Ngày tải lên: 02/11/2022, 11:41

... (35%) were up-regulated by DMI and 471 (36%) were down-regulated by DMI Finally, 148 transcripts (11%) were induced by ␮M ATRA and 240 transcripts (18%) were repressed by ATRA 3.3 High-throughput ... initially discovered in plants and Caenorhabditis elegans by Lee et al and was reported to be associated with the regulation of gene expression and cell differentiation and with guarding organisms ... transcription factor (Srebf1) and retinoid X receptor and alpha (Rxr˛) All primers were synthesized by Integrated DNA Technologies (Leuven, Belgium) and primer sequences are summarised in the

Ngày tải lên: 04/12/2022, 15:03

... synthesized by solid-phase methods on an ABI 433A peptide synthesizer using standard Fmoc chemistry and side-chain protection N-terminal sequencing and MS N-terminal amino acid sequence analysis ... striking feature of conomarphin The l-amino acid to d-amino acid epimerization in a polypeptide chain is quite rare and not well understood, although some d-amino acids have been known for a long ... 1B) Every major peak was collected, repurified, and sequenced The peak that eluted at 53 corresponded to a novel 15 amino acid peptide with the sequence DWEYHAHPKONSFWT, where O is hydroxylated

Ngày tải lên: 18/02/2014, 17:20

... patterns in the amino-acid composition of hyperthermophilic proteins is the bias against thermally labile amino-acid residues. This pattern is obvious on examination of the amino-acid compositions ... and an increase in charged amino-acid residues (i.e. K, E, and R) in hyperthermophilic proteins. As S and T can catalyze the deamination and backbone cleavage of Q and N residues [48,50], a reduction ... was transformed with the ligation mixture by electroporation and plated on M9 medium containing 0.2% xylose, 0.1% casamino acids, thiamine (500 mg·mL 21 ), and ampicillin (100 mg·mL 21 ). Only the

Ngày tải lên: 22/02/2014, 07:20

... amount of D-amino acids produced was determined by the Chirobiotic T HPLC method and the appropriate D-amino acids were used as the standards RESULTS Identification and phylogenetic analysis of ... Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions Ann Rev Biochem 62, 797–821 53 Stadtman, E.R (1992) Protein oxidation and aging Science ... faecalis DA1 N-acyl–D-amino acid amidohydrolase; V paradoxus Iso1: Variovorax paradoxus Iso1 N-acyl-D-amino acid amidohydrolase; P abyssi: Pyrococcus abyssi N-acyl-D-amino acid amidohydrolase;

Ngày tải lên: 08/03/2014, 16:20

... regu- lated by Lys47 acetylation. Therefore, we substituted Lys47 with three different amino acids, i.e. acidic, neu- tral and basic amino acids. The fact that eIF5A activ- ity is impaired partially by ... encode two isoforms with highly simi- lar amino acid sequences (92% amino acid sequence identity). Although the two genes are reciprocally regu- lated by oxygen [46], either of the two yeast proteins can ... site-directed mutagenesis of each conserved amino acid, and by truncation, tested them as substrates for DHS and DOHH, and assessed their activities in supporting growth and protein synthesis in an eIF5A

Ngày tải lên: 16/03/2014, 06:20

... CMP, dCMP and UMP as variable substrates Substitution by hydrophobic side chains of the four amino acids demonstrated by crystallography as inter- acting with the cytosine moiety of CMP and dCMP, always ... the LID and the NMP bind [1]. A characteristic of bacterial CMP kinases is an extension of the NMP bind domain by 40 amino acid residues forming a three-stranded antiparallel b sheet and two ... or dCMP showed that discrimination between CMP and UMP is achieved by Ser36, Arg110 and Asp132, which form hydrogen bonds with the amino group and the N3 atom of the cytosine (Fig. 1). This study

Ngày tải lên: 16/03/2014, 10:20

... confirmed to have a d-amino acid, and synthetic or expressed versions with both l-amino acids and d-amino acids at the correct locus in order to predict when and where d-amino acids may be found ... substitution), whereas the sequences of r11a and r11c differ in seven out of 12 amino-acid positions, including the amino acid Phe44 in r11a which is racemized Thus, if there were a stringent sequence determinant ... correct amino-acid sequences of conotoxins r11b and r11c have d-Phe and d-Leu at positions 44 and 42, respectively Henceforth, we will refer to the d version of the peptides simply as r11b and r11c,

Ngày tải lên: 16/03/2014, 22:20

... with authentic synthetic sequences and/or by amino acid sequencing and molecular mass determination by M ALDI-TOF mass spectrometr y. Results Correlation between structure and activity of CPB isoenzymes ... omprising an 18 amino acid pre-region that is thought to be rapidly removed by a signal peptidase upon transfer into the endoplasmic reticu- lum,a106aminoacidpro-region,a218aminoacidmature domain ... of peptides by CPB3 and H84Y, and, for comparison, also the k cat /K m and K i values of rCPB2.8. The substrate i nhibition w ith t he peptides containing hydrophobic and non-charged amino acids that

Ngày tải lên: 23/03/2014, 13:20

... family. Abbreviations ATG, amino acid transporter glycoprotein; CSR, conserved sequence regions; GH, glycoside hydrolase; HAT, heteromeric amino acid transporter; hcHAT, heavy-chain subunits of heteromeric amino acid ... subunit of these amino acid transporters from the enzymes involved in the metabolism of starch and related saccharides. Results and Discussion Evolutionary relationships and sequence structural ... our knowledge further. Materials and methods As a first step, all available sequences of hcHATs and hcHAT-like proteins were collected (Table 1) using the amino acid sequences of human 4F2hc (GenBank accession...

Ngày tải lên: 18/02/2014, 13:20