ttc0096 by nterminal amino acid sequence analysis and malditofms

Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc

Ngày tải lên : 31/03/2014, 01:20

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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

Ngày tải lên : 18/02/2014, 13:20

... family. Abbreviations ATG, amino acid transporter glycoprotein; CSR, conserved sequence regions; GH, glycoside hydrolase; HAT, heteromeric amino acid transporter; hcHAT, heavy-chain subunits of heteromeric amino acid ... subunit of these amino acid transporters from the enzymes involved in the metabolism of starch and related saccharides. Results and Discussion Evolutionary relationships and sequence structural ... our knowledge further. Materials and methods As a ﬁrst step, all available sequences of hcHATs and hcHAT-like proteins were collected (Table 1) using the amino acid sequences of human 4F2hc (GenBank accession...

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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efﬁciency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

Ngày tải lên : 18/02/2014, 17:20

... being proline, aspartic acid, serine or alanine. Simple and double FNR mutants of amino acids C266 and L268 were obtained and characterized. It was observed that alteration of the amino acid volume decreases ... effect is probably caused by amino acids C266 and L268, which face the other side of this tyrosine. Simple and double FNR mutants of these amino acids were obtained and characterized. It was ... the R amino acid groups introduced by mutations was calculated following the standard radii and volumes calculated by Tsai et al. [32], assuming a reduced state of the cysteine. Amino acid hydropathy...

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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

Ngày tải lên : 08/03/2014, 08:20

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Ecological Assessment of Water Quality by Three-species Acute Toxicity Test and GC/MS Analysis - A Case Study of Agricultural Drains

Ngày tải lên : 05/09/2013, 10:15

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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

Ngày tải lên : 18/02/2014, 13:20

... that require tRNA for amino acid activation, the potential for misrecognition of related amino acids has been investigated [9–13] and modulated by amino acid replacements and active site redesign ... tRNA and 0.35 nmol unlabelled tRNA was aminoacylated in a 10 lL total volume containing 50 mm Hepes pH 7.5, 10 mm MgCl 2 and 2.5 mm ATP together with aminoacyl-tRNA synthetase and amino acids ... on the ﬁdelity with which aminoacyl-tRNA synthetases (EC 6.1.1.x) recognize their cognate amino acid and tRNA substrates [1]. The mechanism(s) by which the family of aminoacyl-tRNA synthetases maintains...

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

Ngày tải lên : 18/02/2014, 17:20

... synthesized by solid-phase methods on an ABI 433A peptide synthesizer using standard Fmoc chemis- try and side-chain protection. N-terminal sequencing and MS N-terminal amino acid sequence analysis ... striking feature of conomarphin. The l -amino acid to d -amino acid epimerization in a polypeptide chain is quite rare and not well understood, although some d -amino acids have been known for a long ... available online: Fig. S1. Natural and synthetic conomarphin with all l -amino acids on HPLC. Fig. S2. The fragments of natural conomarphin (A) and the synthetic l-Phe13-conomarphin (B) cleaved by trypsin on an...

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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

Ngày tải lên : 18/02/2014, 18:20

... contamination from xylem, and vice versa. Glx (glutamine and glutamate: ﬁve carbon amide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were found to be the ... Light) and 24.6 ± 1.5 (xylem, Dark). The standard errors for individual amino acid contents are of the same order of magnitude as those of the total amino acid contents. Amino acid metabolism and ... Amino acid composition in leaves, and amino acid percentage ratio in the phloem exudates and leaves and xylem bleeding sap and leaves in the light and dark. The amino acid composition in leaves is...

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Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

Ngày tải lên : 19/02/2014, 12:20

... before as important step in the biosynthesis of the lantibiotics epidermin and mersacidin catalyzed by the LanD enzymes EpiD and MrsD, respectively [8–10]. Flavin- dependent oxidative decarboxylation ... pQE12 and sequence analysis revealed a single point mutation within the AAVAD motif of CoaB proteins, namely Ala275 is exchanged for Thr275. (B) Dfp and Dfp A275T (¼ Dfp-1) were enriched by anionic exchange ... characterization of the dfp-1 mutation, the dfp-1 gene was cloned into pQE12, sequenced and overexpressed. Sequence analysis revealed that dfp-1 has a point mutation in codon 275 of the dfp gene, substituting...

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Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

Ngày tải lên : 20/02/2014, 01:20

... formed by amino acids 1–141. In this study, we used site-directed mutagenesis to generate point mutations and truncations around this position to explore the above prediction. As shown by Chen and ... truncations and point mutations to C-terminal residues on SNase stability and conformation integrity was exam- ined by subjecting these mutants and wild-type proteins to CD [11,12] and differential ... unfolded by lowering its pH (for example, from pH 7 to pH 2). About 2.5 protons are associated with the key glutamic amino acid residues at positions 75 and 129. This association between protons and...

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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf

Ngày tải lên : 22/02/2014, 07:20

... form, but could be reactivated by denaturation and refolding, either by SDS and refolding by dilution into a buffer with 1% BSA, or by guanidinium chloride and refolding by dialysis against NaCl/P i . ... stressed and the relaxed confor- mations [15]. The network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in b strand 3A, and position 334 in b strand 5A (Fig. ... a helices D and E forms a ﬂexible joint, and b strands 3A and 5A slide apart in a shutter-like manner over the underlying a helix B [14]. The central part of b strands 3A and 5A and the N-terminal...

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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Ngày tải lên : 22/02/2014, 07:20

... and an increase in charged amino- acid residues (i.e. K, E, and R) in hyperthermophilic proteins. As S and T can catalyze the deamination and backbone cleavage of Q and N residues [48,50], a reduction ... patterns in the amino- acid composition of hyperthermophilic proteins is the bias against thermally labile amino- acid residues. This pattern is obvious on examination of the amino- acid compositions ... the divalent metal ion in sugar binding, ring opening, and isomerization by D-xylose isomerase: replacement of a catalytic metal by an amino acid. Biochemistry 33, 1488–1494. 30. Hartley, B.S.,...

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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several speciﬁc biological processes through GCN2-dependent and GCN2-independent pathways ppt

Ngày tải lên : 07/03/2014, 03:20

... eight genes regulated by amino acid starvation using quantitative RT-PCR. We selected genes that were either repressed (Hmgcs1) by amino acid starvation or induced by amino acid starvation in a ... mammals: (a) the target genes and biological processes regulated by amino acid avail- ability, and (b) the signaling pathways that mediate the amino acid response. Using large-scale analysis of gene expression, ... gene by the amino acid response and the endoplasmic reticulum stress response pathways occurs by common genomic elements. J Biol Chem 275, 26976–26985. Regulation of gene expression by amino acid...

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Báo cáo khoa học: Phytoene synthase genes in tomato (Solanum lycopersicum L.) – new data on the structures, the deduced amino acid sequences and the expression patterns doc

Ngày tải lên : 07/03/2014, 05:20

... and Psy2 genes and also provide the deduced complete amino acid sequences of the two enzymes, together with new insights into the transcriptional regulation of Psy1 and Psy2 in chloroplast- and ... deduced amino acid sequences and the expression patterns Giovanni Giorio, Adriana Lucia Stigliani and Caterina D’Ambrosio Metapontum Agrobios, Metaponto, Italy Fruits are the mechanism by which ... almost identical and six introns being much more vari- able. For Psy1 and Psy2, respectively, the sequenced regions were 4527 and 3542 bp long, the coding sequences were 1239 bp and 1317 bp long, whereas...

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Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

Ngày tải lên : 07/03/2014, 05:20

... lM ( ), 10 lM ( ) and 20 lM (·) OAB; and (F) NAT as indicated and 0 lM ( ), 5 lM ( ), 10 lM ( ) and 20 lM (·) OAB. Inhibitor-binding sites of L -amino acid oxidase S. Mandal and D. Bhattacharyya 2088 ... indicated and 0 lM ( ), 1 lM ( ), 2 lM ( ), 5 lM (·), 10 lM (h) and 20 lM (D) OAB; (B) NATA as indicated and 0 l M ( ), 5 lM ( ), 25 lM ( ) and 35 lM (·) OAB; and (C) NAT as indicated and 0 lM ... followed at 450 nm. Reference FAD and eluted cofactor were collected and lyophilized for ESI MS analysis. Enzyme assay l -Amino acid oxidase activity was followed by a coupled assay [22]. Hydrogen...

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Báo cáo khoa học: Substrate speciﬁcity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

Ngày tải lên : 07/03/2014, 16:20

... [1,2,4,7]. Moreover, PAT1 and PAT2 mediated amino acid i nflux leads to a pronounced intracellular a cidification [2,9] by cotransport o f t he zwit- terionic amino acid substrates and protons with a ... the amino group as in sarcosine. Interestingly, all tested prolines ( L -Pro, D -Pro and L -OH-Pro) are recognized as high affinity substrates by PAT2 with D -Pro as the only D -amino acid, and L -OH-Pro ... spectrum that includes not only t he amino acids glycine, alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent affinities...

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Báo cáo khoa học: Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

Ngày tải lên : 07/03/2014, 21:20

... and K9, and of G129 with K110 and K133. Other charged amino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129. H M. Chen et al. Local stability and key acidic amino acids ... Calculation of protein extinction coefﬁcients from amino acid sequence data. Anal Biochem 181, 319–326. Local stability and key acidic amino acids in staphylococcal nuclease H M. Chen et al. 3974 ... Privalov and Potekhin [6]. Local stability and key acidic amino acids in staphylococcal nuclease H M. Chen et al. 3970 FEBS Journal 272 (2005) 3967–3974 ª 2005 FEBS nuclease studied by site-directed...

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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx

Ngày tải lên : 07/03/2014, 21:20

... cDNA of acidic DE25 encoded a 137 -amino acid protein and a 22 -amino acid signal peptide (AB201776). A partial cDNA of acidic DE29 lacked the 5¢-region and its N-terminal sequence determined by Edman ... Tyr (10–15%), Gly (16–19%), and Asp (10–12%). Proteins in the basic QH group were abundant in Glu (13–16%) and His (7%) as determined by amino acid analysis, but their partial amino acid sequences indicated ... Asp (14%) and Glu (10–14%). N-Terminal amino acid sequence analysis of HMM chitin-binding proteins Eleven of 37 spots observed on 2D SDS ⁄ PAGE were resistant to sequence analysis by Edman degradation, presumably...

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Báo cáo Y học: Restoring enzyme activity in nonfunctional low erucic acid Brassica napus fatty acid elongase 1 by a single amino acid substitution pdf

Ngày tải lên : 08/03/2014, 09:20

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Báo cáo Y học: Identiﬁcation and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf

Ngày tải lên : 08/03/2014, 16:20

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