triage in different zones of the scene

... pSlgt3K Cloning strain Wild-type strain Strain with EZ::TN insertion in lgt3 gene (1203-bp) Strain with EZ::TN insertion in lgt3 gene (901-bp) lgt3 knockout mutant strain of O35E Invitrogen (24) ... TA cloning vector Cloning vector Kanamycin-resistant gene lgt3 cloned into pCR2.1 EcoRI-SalI lgt3 fragment cloned into SK(+) EcoRI-blunted kanamycin-resistant gene inserted into blunted HindIII ... catarrhalis strain O35E using primers 25 and 26 (Table 1, Fig 2) The PCR product was cloned into pCR2.1 using a TOPO TA cloning kit (Invitrogen, Carlsbad, CA) to obtain pCRlgt3 The insert was released...

Ngày tải lên: 07/03/2014, 05:20

... level early in life In this sense the finding in the literature that stress adversely affects the probability of twinbirths (see Subsection 4.1.2) is in line with our finding that twinning rates are ... the individual is born under adverse economic conditions Individual-specific factors dominate stronger the individual in born under better conditions This is an interesting result In short, individual-specific ... (i.e # twin births / # births) and not by fluctuations in the birth rate Interestingly, the yearly deviation in the twinning rate is significantly positively related to the business cycle indicator...

Ngày tải lên: 17/03/2014, 08:20

... deformation and results of Ar‐Ar dating in this  area  in order  to  constrain  in detail  the  spatial  metamorphic  evolution  and  interpretation  of  geodynamic setting of the Indochina.   2.  Geological  ... Fig. 1. The study area and its location in regional geological setting: A. Position of studied area and main  boundaries involved in the Indosinian Orogenic period between Gondwanian crustal blocks (adapted from [6,  11]); B. Sketch map of the major Indosinian strike‐slip shear zones in Vietnam (adapted from [5, 6]).   ... metamorphism in Kon Tum massif [9,10, 19, 12,  20].  The  ages  obtained  in this  research  also  mark  an  Indosinian  event  in different places.  However,  the  presence  of  an  intense ...

Ngày tải lên: 22/03/2014, 12:20

... stretching vibration of QA by  60 cm)1 [10,11], indicating strong asymmetric binding of UQ10 at the QA site, in contrast to symmetric, weaker binding of UQ10 at the QB site [12,13] To explain the ... binding sites However, there are a large variety of contradicting conformations in the different structural models: in refs [1,2] one methoxy group is shown within the plane of the quinone ring ... but strong binding to His M219 at QA (Fig 5), and His L190 at QB combined with electrostatic interactions with the Fe2+ ion and with further amino-acid side chains in the QA binding niche (e.g...

Ngày tải lên: 23/03/2014, 21:20

... dragging out and burning the cut material Selective individual and scattered scrub clearing with PS Individual selection and scrub clearing using PS consists in the selective disposal of individual ... was to find out the costs of basic methods of forest land maintenance in protective zones of high-voltage power lines (HVPL) 400 kV in the protective zone of 70–80 m in width, and 220 kV in the ... growing must be avoided, cut scrub among growing individuals is more difficult to bring down, it is worse to manipulate with, work is more risky All this is reflected in an increase in the existing...

Ngày tải lên: 07/08/2014, 10:21

... classification in canine oocytes stained after in vitro maturation Classification Description Germinal vesicle (GV) Germinal vesicle (nuclear envelope) still intact; scattered chromatin Germinal vesicle ... compound, to culture medium can increase the cysteinmediated GSH synthesis in bovine embryo produced in vitro Similarly, addition of β-ME increased intracellular GSH in mouse lymphocytes [37] It ... oocytes matured in a protein-free medium containing epidermal growth factor Theriogenology 2000, 54, 787-797 Carson RS, Zhang Z, Hutchinson LA, Herington AC, Findlay JK Growth factors in ovarian function...

Ngày tải lên: 07/08/2014, 18:20

... viral replication in these organs indicate that yet to be known receptor determinants might be involved These findings also indicate the shortcomings of receptor studies using lectin histochemistry ... tonsils analyzed in this study does not necessarily indicate absence of infection with influenza viruses especially following infection with highly pathogenic isolates indicating again that receptor ... ducks of the age groups was an interesting finding, especially considering the dominant presence of a2,3SA-gal receptors in epithelial cell of the large intestine The presence of avian type receptors...

Ngày tải lên: 12/08/2014, 04:21

... to be increased in sarcoidosis [15] as well as in the bronchoalveolar lavage fluid obtained from patients with interstitial lung diseases [16] and in developing lung [17] Cigarette smoke induces ... fibroblasts, using antibodies against alpha-smooth muscle actin (1:1000) and vimentin (1:1500) The immunohistochemical staining was performed as previously described [15,17] using the Histostain-Plus ... Immunohistochemical staining of the precursor protein of collagen I and III was present mainly extracellularly, being visible as linear and reticular fibers or as more intensive bands in the subepithelial...

Ngày tải lên: 12/08/2014, 13:22

... "donor" block using a thin-wall stainless tube from a precision instrument (TMA-100, Chemicon, USA) and were transfered by a solid stainless stylet into defined array coordinates in a 45 * 20 mm ... corroborated ING4 down-regulation in IPF patients compared to controls and COP subjects, on a protein level as well (Figure 4) In particular, ING4 showed strong staining intensity within normal ... statistical difference in staining intensity was observed between COP and control lung samples in ING4 expression (Figure 4) On the contrary, ING4 was almost absent within IPF lung in the majority of...

Ngày tải lên: 12/08/2014, 14:20

... portion of classroom speaking time when learners are practicing an intonation contour or trying to pinpoint a certain vowel sound, etc - Intensive: includes any speaking performance that is designed ... mainly on developing reading and writing skills The students of English major classes use a curriculum which lays great emphasis on four skills including listening, speaking, reading and writing ... other kinds of activities such as interviewing, discussing, and problem solving and decision-making because they were afraid of making noise In fact, I want to create a funny and interesting atmosphere...

Ngày tải lên: 07/11/2012, 14:50

... France is in Taiwan; and Wellcome is in Taiwan, Hong Kong, Mainland China and Singapore Major Japanese department stores are in most Asian cities According to Dairy Farm, in Hong Kong in 1980, ... Commercial center in Manila, is also rushing the nine-hectare Ayala Center in the Cebu Business Park (AR, Feb 1994) In the Philippines, the wholly-owned foreign retailer is , in principle, non-existent ... there is again opposing behavior in prior intention of going supermarkets between the two groups, describing in table 4.9 The option “plan before going” appeared much more often in heavy-users...

Ngày tải lên: 13/04/2013, 10:30

... understanding and defining modality Many researchers are interested in studying modality One of the authors who are interested in defining modality is V Vinogradov According to V Vinogradov (1977), ... doing something Similarly must in B1 is different from must in B2: Must in B1 conveys possibility or logic inference; must: in B2 has obligatory meaning Therefore, may in A1 and must in B1 show ... are many disagreements of linguists about the modality Therefore, studying on modality plays an important role in linguistics in general and in teaching foreign language in particular Besides that,...

Ngày tải lên: 18/12/2013, 10:08

Ngày tải lên: 18/02/2014, 11:20

... Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a manner similar ... blocked by incubating sections in 0.3% hydrogen peroxide in methanol for 30 at room temperature Slides were then washed and incubated in a blocking solution according to the manufacturer’s instructions ... mainly expressed during larval metamorphosis, in the mantle edge and the digestive gland During the reproductive cycle, expression was high in gonads during the postspawning period but not in...

Ngày tải lên: 19/02/2014, 00:20

... conserved arginine (Arg622 in HalomarCPc) in the corresponding position, indicating that these domains lost their ability to co-ordinate the heme Further, a conserved tryptophan (Trp311 in HalomarCPn, ... of coding sequences currently in the database may affect this value Interestingly, none of the known fungal genomic DNA sequences in which KatG is included (listed in Table 1) contain an intron, ... bootstrap support in all main nodes is strong; only in some minor nodes is refining of the particular species within groups moderate In the case of PUZZLE, the likelihood mapping analysis also...

Ngày tải lên: 19/02/2014, 16:20

... sperm-binding activity using in vitro competition and sperm-bead binding assays [18] We deduced the amino acid sequence of the bovine ZPA by cDNA cloning and sequencing, and revealed that bovine ... chains in bovine ZPA in order to fill a gap in the structural information on the zona glycoproteins As with porcine ZPB and ZPC, bovine ZPA possesses three N-glycosylation sites (Fig 2) determined ... acids in bovine sperm-zona binding has not been demonstrated Bovine sperm-binding activity is mainly ascribed to the high-mannose-type chain with five mannose residues [15] Molar ratio The ZP domain...

Ngày tải lên: 21/02/2014, 03:20

... circumstances in spite of wide variations in the way that care is provided in different societies Families play a critical role especially in developing countries, where the elderly population is increasing ... performing well in the instrumental ADL (75–82%) were only slightly reduced The authors conclude that in general the rural elderly were in better condition than the urban elderly, being in general ... rapidly But in all societies families are typically generous in providing care In Indonesia the extended family system is still practised and the families regard the placing of the elderly in an ‘old...

Ngày tải lên: 05/03/2014, 21:20