transport of biotin to human milk

... Štotal À ½Yb3þ Šbind Binding of Yb3+ to apo-hTf ¼ ½Yb3þ Štotal À ½Yb3 Æ TfŠ The thermodynamics of binding of Yb3+ to apoTf Binding of Yb3+ to apo-Tf was investigated by addition of aliquots of ... addition of various amounts of molar ratios of Yb3+ (as YbCl3) The two absorption bands at 242 and 295 nm are indicative of Yb3+ binding to the specific iron sites of Tf Molar ratio from bottom to top: ... ICP-AES was also used to study the binding ratio of Yb3+ to apo-Tf After addition of 2.0 and 2.5 mol equiv of Yb3+ to apo-Tf, the final ratios of Yb3+ to Tf, after removal of low molecular mass...

Ngày tải lên: 08/03/2014, 09:20

... number of moles of berberine bound to mol of quadruplex (Cb ⁄ Cqua), n is the number of equivalent binding sites, and Kb is the affinity of ligand for those sites [52] Data were fitted to the McGhee– ... presence of different amounts of quadruplex were recorded in order to study the binding event Figure shows the effect of successive addition of quadruplex on the fluorescence emission spectra of berberine ... intensity at 522 nm due to addition of quadruplex solution was used to construct the binding isotherm (inset of Fig 3) Analysis of this isotherm following : binding stoichiometry using Eqn (7)...

Ngày tải lên: 16/03/2014, 06:20

... that this particle was extremely useful as a vector to target human hepatocyte in vivo exploiting the character of infectivity of HBV limited to human liver [6] Because this recombinant particle ... efficient way to prepare a BNC to convey foreign proteins Thus, fusing a suitable protein to the tail should be a convenient way of preparing a BNC that delivers proteins to human hepatocytes Results ... medium (b), 100 ng of EGFP (c) and a mixture of ng of BNC with 100 ng of EGFP (d), and ng of L(n45)-FLAG-EGFP particles from the conditioned medium (e) were added to · 104 cells of HepG2, respectively...

Ngày tải lên: 23/03/2014, 15:20

... clones and cloned into vector pAcEGFP-N1, with the addition of a stop codon to prevent expression of the EGFP tag A Gly to Ala mutation at the second amino acid was introduced into CMO2.5 Nef by ... of human immunodeficiency virus type enhances viral particle release: a Vpu-like factor? J Virol 1996, 70:820-829 10 Le Tortorec A, Neil SJ: Antagonism to and intracellular sequestration of human ... plasmids were transfected into HeLa cells (range to μg), to confirm that their lack of anti-tetherin activity was not simply due to lower levels of expression As a control, μg of group M Vpu-FLAG was...

Ngày tải lên: 13/08/2014, 01:21

... 928 OFFICE OF WOMEN’S HEALTH ‘‘(a) ESTABLISHMENT.—There is established within the Office of the Director of the Agency for Healthcare Research and Quality an office to be known as the Office of ... 14 the Office of the Commissioner of the Food and Drug Ad15 ministration an office to be known as the Office of Wom16 en’s Health (referred to in this section as the ‘Office’) 17 The Office shall ... 24 lished within the Office of the Administrator of the Health 25 Resources and Services Administration an office to be 26 known as the Office of Women’s Health (referred to in •HR 1784 IH 13...

Ngày tải lên: 13/02/2014, 07:20

... OFFICE OF WOMEN’S HEALTH ‘‘(a) ESTABLISHMENT.—There is established within the Office of the Director of the Centers for Disease Con5 trol and Prevention, an office to be known as the Office of ... within the Office of the Administrator of the Health 12 Resources and Services Administration, an office to be 13 known as the Office of Women’s Health The Office shall 14 be headed by a director who ... within 20 the Office of the Director, an Office of Women’s Health 21 and Gender-Based Research (referred to in this section 22 as the ‘Office’) The Office shall be headed by a director 23 who shall...

Ngày tải lên: 13/02/2014, 07:20

... analysis of warfarin binding to human serum albumin: anatomy of drug site I J Biol Chem 276, 22804–22809 26 Chuang VTG & Otagiri M (2002) How fatty acids cause allosteric binding of drugs to human ... Otagiri M & Perrin JH (1980) The effect of albumin conformation on the binding of warfarin to human serum albumin The dependence of the binding of warfarin to human serum albumin on the hydrogen, ... properties of the Mn(III)heme–HSA adduct in the presence of myristate are similar to those of the B conformational state of HSA, suggesting that myristate binding to one or more modulatory sites...

Ngày tải lên: 20/02/2014, 02:22

... function of human cells The discovery of five enzymatically distinct forms of LF, their cytotoxicity, and the ability of some of them to induce tumor cell apoptosis should contribute to the understanding ... cytotoxic to human cancer cells [40] because of induction of apoptosis by multimeric a-lactalbumin We studied the effects of various subfractions of LF with catalytic activities on the growth of ... Interaction of human milk lactoferrin with ATP Biochemistry (Mosc) 63, 67–75 Semenov, D.V., Kanyshkova, T.G., Buneva, V.N & Nevinsky, G.A (1999) Human milk lactoferrin binds ATP and dissociates into monomers...

Ngày tải lên: 21/02/2014, 00:20

... and Toc159 system used by proteins involved in photosynthesis [36] The Toc159 family in Arabidopsis consists of four proteins: Toc159, Toc132, Toc120 and Toc 90 [37] It is possible that one of ... receptor Toc64 [23] OM64 did not appear to play any specific role in the import of GR compared to mitochondrial precursor proteins One protein that was identified to play a role in the import of ... required to avoid mis-sorting of proteins between mitochondria and chloroplasts The structures of binding of targeting peptides to mammalian and plant Tom20s not only revealed the molecular details of...

Ngày tải lên: 07/03/2014, 03:20

... include the Arabidopsis (Rboh), rice (RbohAOsp), tomato (RbohTomato) and induced in tomato by whitefly (RbohTomIn), potato (Strboh) and Dictyostelium (DictoNox2) The pig and rabbit Nox1 are incomplete ... reported to be greatly reduced by the mutation of His115 to Leu compared to that observed for wild-type Nox2 [13,14] To assess the contribution of histidines 119 and 111 to the conduction of protons ... as an inhibitor of the influenza A M2 proton channel and hence prevents the uncoating of the viral particle and release of its genome into the cell [18,23] Therefore, the influx of protons through...

Ngày tải lên: 07/03/2014, 16:20

... contributions of system y+ and y+L to the overall rate of L-arginine transport under physiological conditions it would be suitable to measure the transport in the presence of plasma concentrations of competing ... be due probably to the presence of the system b+ [16] but this component accounts for 5–7% of the total arginine influx Thus its contribution to arginine influx seems to be minor To clarify the ... by 35% of the total in close correlation with the N-ethylmaleimide effect on L-arginine uptake Moreover the addition to platelet of L-leucine, able to competitively inhibit L-arginine transport...

Ngày tải lên: 08/03/2014, 02:20

... components binding to this enzyme need to be identi®ed to understand the cytoplasmic location of the enzyme in 15PC3 cells This enzymatic location appears to be a necessity for activity towards ODNs ... a number of sequence-independent artefacts, most of which are attributable to their ability to bind a number of heparin-binding proteins [28] In our search for allele-speci®c inhibitors based ... as the level of full-length mRNA can be assayed To avoid scoring possible artefacts of the ODN delivery system and chemistry-related toxicity, we used liposomal delivery of PS-ODNs to the cells...

Ngày tải lên: 08/03/2014, 10:20

... containing cytoskeleton proteins were Fig Aggregation in response to thrombin and convulxin induces relocalization of RAS from the cytoplasmic to the cytoskeleton fraction (A, B, C and D) Washed human ... cytoplasmic to the cytoskeleton fraction could suggest an involvement of RAS during cytoskeleton rearrangement of platelets Our study shows that in platelets RAS is not sufficient by itself to ... mediation of the phosphorylation of the ITAM motif of the Fc receptor c chain [11,12] As a principal mechanism, the activation of RAS leads to the activation of ERK1-2 by sequential activation of RAS–...

Ngày tải lên: 08/03/2014, 16:20

... Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human ... Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Size (bp) 1 2 3 4 5 6 7 8 9 10 10 11 11 12 ... close to that of the mouse Muc4 (Table 1) The size of intron of the human MUC4 has been estimated to be  15 kb by restriction mapping [12] and to be at least 20 kb by our analysis of the human...

Ngày tải lên: 08/03/2014, 23:20

... •HR 1784 EH of the Director of the Centers for Disease Control and Prevention, an office to be known as the Office of Wom3 en’s Health (referred to in this section as the ‘Office’) The Office shall ... the Office of the 24 Commissioner, an office to be known as the Office of 25 Women’s Health (referred to in this section as the Of HR 1784 EH 17 fice’) The Office shall be headed by a director ... Office 17 of the Administrator of the Health Resources and Services 18 Administration, an office to be known as the Office of 19 Women’s Health The Office shall be headed by a director 20 who...

Ngày tải lên: 14/03/2014, 14:20

... (2002)’s database of coherenceannotated texts 3.1.2 Kinds of coherence relations We assume a set of coherence relations that is similar to that of Hobbs (1985) Below are examples of each coherence ... speakers of English and were naïve as to the purpose of the study (i.e none of the subjects was familiar with theories of coherence in natural language, for example) Participants were asked to read ... of each algorithm and human sentence ranking for the 15 texts MarcuAvg refers to the version of Marcu (2000)’s algorithm where we calculated sentence rankings as the average of the rankings of...

Ngày tải lên: 17/03/2014, 06:20

... a temperature of 900 K with a relative cooling factor of 0.95 ⁄ cycle, in order to reach the temperature of K in 100 cycles [56–58] Binding of Fe(III)heme to HSA in the presence of either drug ... induce the release of FA7bound drugs with the concomitant intoxication of the patient [7,35,51] Moreover, binding of drugs to FA2 reduces the affinity of FA7 and increases the affinity of FA1 ligands ... one of multiple binding modes of nevirapine to HSA previously distinguished on the basis of fluorescence quenching of Trp214 (located in close proximity of FA7) [51] Indeed, nevirapine binds to...

Ngày tải lên: 23/03/2014, 07:20

... synthesis of the precursor molecules of peptide bi®dus factors is coupled to the bi®dogenic activity of human milk; the bi®dogenic activity of colostrum is higher than that of mature milk [15], ... are from equivalent positions of the C-terminus of lactoferrin hLACFR-Ia corresponds to the generally known lactoferrin sequence hLACFR-Ib is obtained from a lactoferrin variant with an arginine ... 2002 Human milk peptides as bi®dus factors (Eur J Biochem 269) 713 Fig Puri®cation of bi®dogenic peptides from human milk by consecutive chromatographic steps (A) Reversed-phase HPLC separation of...

Ngày tải lên: 23/03/2014, 21:21