translocation assay for monitoring activation of gpcr in living cells

... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated ... ogttST::Cmr) strain (deficient in O6-methylguanine DNA methyltransferase) sensitive to alkylating chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences ... 1,8-Dinitropyrene 1,6-Dinitropyrene 1-Nitropyrene NIP 4-Nitroquinoline-N-oxide Malathion MEP Maneb Aniline N,N-Dimethylformamide Methomyl Trifluralin 1,4-Dioxane Benzophenone EPN Pyrene 2-Aminoanthracene...

Ngày tải lên: 05/09/2013, 08:40

... existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely related homologs infecting different ... Research Center [18] In the Tulane study, a QPCR assay developed against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques ... curve obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount (1 µg) of genomic DNA DNA from MmuA01111 which was naturally infected with RRV was assayed in duplicate in four-fold...

Ngày tải lên: 18/06/2014, 22:20

... existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely related homologs infecting different ... Research Center [18] In the Tulane study, a QPCR assay developed against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques ... curve obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount (1 µg) of genomic DNA DNA from MmuA01111 which was naturally infected with RRV was assayed in duplicate in four-fold...

Ngày tải lên: 20/06/2014, 04:20

... The assay was found to be effective in detecting as few as 10 organisms per 100 mg of feces This assay will be useful in identifying animals in their early clinical stage, shedding low numbers of ... RT- PCR product of RNA extracted from fecal samples containing 10 organisms of MAP RFR: reflectometer reading, CFU: colony forming units A biosensor assay for the detection of Mycobacterium ... containing mM EDTA) were diluted in hybridization buffer, and denatured o at 95 C for To this mixture, μl of liposomes for o each well was added and incubated at 60 C for 20 One hundred μl of this...

Ngày tải lên: 07/08/2014, 23:22

... Slides were rinsed for minutes in distilled water, immersed for minute in 95% ethanol, and dried with a stream of nitrogen gas Page of 14 (page number not for citation purposes) Forty-two of the most ... 95°C step for 10 minutes (for Taq activation) , followed by 40 cycles of 95°C for 15 seconds, 60°C for minute, and then a final melting curve analysis with a ramp from 60°C to 95°C for 20 minutes ... [6] These findings point toward an important role of innate immune cells, particularly neutrophils, in this disease Hence, we have proposed that the pathogenesis of JRA involves complex interactions...

Ngày tải lên: 09/08/2014, 08:22

... measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 ... http://www.virologyj.com/content/7/1/253 Page of 10 Figure Detection of HPV infection by PCR in OSMF and OSCC cases Gel figure A represent the ethidium bromide-staining in 2% agarose gel and showing presence of HPV infection in OSMF ... negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio at 1.0 correspond to viral DNA load of 5,000 copies/ml of 1pg/ml at a threshold of finding a clinical disease...

Ngày tải lên: 12/08/2014, 01:22

... obtained from the breeding facility of the Institute of Poultry Sciences in Sichuan Agricultural University, China Animals were bred and maintained in an accredited facility at the Institute of ... Fifty goslings were randomly divided into groups In brief, a group of 40 goslings were orally infected with GPV CHV strain, using 0.1 mL of 103 LD50 per gosling Another group of 10 goslings was ... signal, suggesting that the established FQ-PCR assay was highly specific (Figure 2) The intraassay and interassay CV of this established FQPCR was in the range of 0.8-3% for most of the dynamic...

Ngày tải lên: 12/08/2014, 04:20

... analysis of influenza A is becoming increasingly prevalent in clinical microbiology laboratories, and is essential for public health surveillance of circulating strains, determination of annual vaccine ... city of the CombiMatrix influenza detection system for the detection of an influenza A subtype (either H1 or H3) was 100% The LOD of the Luminex RVP assay was found to be 0.3 TCID50s/mL for influenza ... H, Pauli G: Application of a fluorogenic PCR assay for typing and subtyping of influenza viruses in respiratory samples J Clin Microbiol 2000, 38:1552-1558 Poddar SK: Influenza virus types and...

Ngày tải lên: 12/08/2014, 04:21

... of 1x103 cells/ ml and maintained in culture for days to allow the cells to form a confluent monolayer culture For transport studies cells were seeded in 96-well microtiter scintiplates (PerkinElmer, ... determine the amount of ATP in mg of protein measured from the number of cells per well After 24 h, the microtiter plate was taken for scintillation counting of radioactive [14C]AMG using a MicroBeta ... hSGLT1, suggesting that differences in the aglycon binding site play an important role in the inhibitory effect of this substance In contrast, differences in the sugar binding site resulted in an preferen-...

Ngày tải lên: 26/10/2012, 10:04

... isoform of JNK, whereas sorbitol treatment induced phosphorylation of both the p46 and p55 isoforms To confirm functional activation of the JNK signaling pathway, we performed a solid-phase kinase ... necessary for activation of ERK in response to a number of growth factors, including insulin growth factor-1, platelet-derived growth factor and epidermal growth factor [14,15] Various protein phosphatases ... phenotype of transfected cells after staining with DAPI (Fig 5A) We observed that the floating cells showed condensation of chromatin, a morphological characteristic of apoptosis [27] This was in contrast...

Ngày tải lên: 16/02/2014, 14:20

... composed of an almost infinite number of individual germs, the result of the continued growth of a single organism that was in the drop of milk which was firmly held in place when the gelatin solidified ... Fig 14 Effect of cooling milk on the growth of bacteria Conn[42] is inclined to regard temperature of more significance in determining the keeping quality than the original infection of the milk ... production of a large amount of by-products, as a result of the development of a relatively small amount of cell-life The souring of milk, the formation of butyric acid, the making of vinegar from...

Ngày tải lên: 16/02/2014, 22:20

... higher than those of cyclists, noting that they are also highly variable depending on an individual’s level of fitness and individual choice of intensity of physical exercise In addition to the ... 36 L min-1 (within the range of 24-60 L min-1) and the runner a moderate level of 48 L min-1, both within the range of values reported in the literature [21-23] The commuter ‘dose’ was defined ... 2012, Volume instead of controlling the amount of traffic on the existing roads Congestion charging schemes such as those introduced in Singapore and London, which have lead to a decrease of 40% and...

Ngày tải lên: 18/02/2014, 04:20

... XVI INCOSAI in 1998) • guidelines on best practice for the audit of economic regulation (adopted by XVII INCOSAI in 2001); and • guidelines on best practice for the audit of public/private finance ... regulation INCOSAI XVII asked the Working Group to examine whether further guidance on the new forms of partnership – including issues arising when the state is a minority shareholder in private businesses ... are in place • Obtain an independent, expert valuation of the assets • Ensure that issues of profit sharing (including possible future gains from property sales or refinancing) are addressed...

Ngày tải lên: 18/02/2014, 05:20

... backoff models for combining in- domain and 42 out -of- domain data for the purpose of bootstrapping a part -of- speech tagger for Chinese, outperforming standard methods such as EM i i i Backoff ... consist of 2000 sentences each In order to study the effect of varying amounts of training data we created several training partitions consisting of random selections of a subset of the full training ... differences to the baseline model are shown in boldface (p < 0.05) Set train1 train2 train3 train4 train5 Set train1 train2 train3 train4 train5 German-English baseline backoff BLEU PER BLEU PER...

Ngày tải lên: 22/02/2014, 02:20

... distribution of BsArb43B in the proximity of the active site Arabinotriose in the binding cleft is represented using sticks (B) Detailed view of the binding cleft with arabinotriose (C) Details of the ... hairpin that joins blades IV and V in the N-terminal domain and interacts with the C-terminal domain is shown in red catalytic domain with the Ca trace of BsArb43A from B subtilis (Protein Data ... arabinohexose [15], this loop makes the reducing end of the carbohydrate chain bend towards the solvent, probably optimizing binding of the carbohydrate chain in the cleft, and resulting in trioses...

Ngày tải lên: 15/03/2014, 23:20

... bovine serum albumin, 20% (v/v) normal goat serum in 0.1 M Trisbuffered saline (pH 7.4), and then incubated for h in Trisbuffered saline (pH 7.4) containing PAAF (diluted : 5), 1% (w/v) bovine ... pea seed proteins (CP, control proteins); molecular mass of protein standards is indicated in kDa (Std) (B) Immunoblotting of the same proteins with polyclonal antibody against ferritin (PAAF) (C) ... independent from the binding of the inhibitor protein IF1 Biochim Biophys Acta 1556, 133–141 25 Shevchenko, A & Shevchenko, A (2001) Evaluation of the efficiency of in- gel digestion of proteins by peptide...

Ngày tải lên: 16/03/2014, 18:20

... trafficking, creating increased expenditures for police protection Finally, the cost of federally incarcerated inmates estimated a loss of productivity at $52.9 million and cost of incarceration for ... assessment, treatment, and monitoring requirements for 11 chronic pain (Arnstein & Marie, 20 10) In addition, only 40% of physicians received any training in medical school in identifying prescription ... many contributing factors such as a lack of funding for a national prescription monitoring program (NASPER) and a lack of adequate professional and public education The lack of funding was the...

Ngày tải lên: 17/03/2014, 06:20

... Sub-confluent cells in 6-cm diameter dishes were incubated in DME containing 2% FBS that had been dialyzed against saline and 40 lCi of a mixture of [35S]methionine and [35S]cysteine (1000 Ci ... FRTL cells [24] RT-PCR demonstrated that the FRTL cells indeed expressed a certain level of TRa (Fig 6A) As in the HeLaTR cells, T3 treatment of the FRTL cells significantly increased the level of ... treatment of the HeLaTR cells with T3, overexpression of 4-1BB in the HeLaTR cells led to the activation of caspases Activation of caspases by the overexpression of 4-1BB was not an artefact of overexpressing...

Ngày tải lên: 23/03/2014, 18:20

... SH-SY5Y and HEK293 cells As shown in Fig 5A, the binding of nuclear proteins to GC-boxes and was detected by doublet bands in both cell lines The extent of binding of nuclear proteins to GC-box was ... and in the NOX4 promoter To examine whether Sp1 and Sp3 bind to GC-boxes and in the NOX4 promoter, chromatin immunoprecipitation (ChIP) assays were performed As shown in Fig 6, specific binding of ... that for other GC-boxes When specific binding of nuclear proteins to GC-boxes and was verified, the bands almost completely diminished in the presence of excess unlabeled probes, but not in the...

Ngày tải lên: 29/03/2014, 00:20

... described in (A) UV light but not activation of p38 MAP kinase increases cytoplasmic HuR-binding activity Analysis of protein–RNA binding showed that several complexes were formed when GM-CSF ARE is incubated ... kinase pathway, as determined by phosphorylation of recombinant HSP27 in in vitro kinase assays with cytoplasmic lysates (Fig 5B, lower panel) The half-life of endogenous histone mRNA in the cells ... the indicated plasmids Amounts of plasmid DNA within each experiment were kept constant by adding empty vector For each kinetics of mRNA degradation assay, cells from one dish were trypsinized...

Ngày tải lên: 31/03/2014, 08:20