transgenic expression of therapeutic proteins in arabidopsis thaliana seed

... type of DNA binding domain in ethylene-responsive element binding proteins (EREBPs) in tobacco, exhibiting no structural homology with previously reported DNA binding domains (Ohmetakagi & Shinshi, ... seed germination by inducing ABI5 (ABA-insensitive5), a basic domain/leucine zipper (bZIP) transcription factor (Finkelstein, 1994) RGL2 inhibits Arabidopsis seed germination by increasing ABA ... PAC-mediated inhibition of seed germination During the development of young seedlings, transgenic plants in which RAP2.6 was silenced overcame ABA-induced growth retardation Silencing of RAP2.6...

Ngày tải lên: 11/09/2015, 10:18

... results indicate that lack of MET1 in the male gamete has no effect on imprinting of ATCDC48, PDE120 and MS5-like in the developing seed In contrast, we find that lack of MET1 leads to overexpression ... thaliana seeds, from which we further demonstrate that three are novel maternally expressed imprinted genes in Arabidopsis thaliana seeds Results cDNA-AFLP expression profiling of Arabidopsis thaliana ... At4g01000 Ubiquitin family protein At4g16830 Nuclear RNA-binding protein (RGGA) At4g21270 AT KINESIN At4g29450 Leucine-rich repeat protein kinase, putative At4g33450 Myb domain protein 69 (AtMYB69)...

Ngày tải lên: 11/08/2014, 11:21

... DnaJ proteins are localized in the chloroplasts, most of the genes related to thylakoid membranes were not affected by lacking of one of the small DnaJ proteins (Additional file 4) More interestingly, ... WT by HL treatment, including upregulation of several distinct genes encoding transcription factors, heat shock proteins, DnaJ proteins as well as antioxidant and redox proteins, among others (Additional ... activity of Rubisco is compromised in the DnaJ mutants, especially in j8 Considering the general function of the DnaJ proteins as chaperone proteins [1], the reduced amount of Rubisco Activase in the...

Ngày tải lên: 12/08/2014, 03:21

... by hematoxylin and eosin staining We examined the expression of these proteins in a cancer-free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa ... The higher level of Mcm expression was detected not only in total cellular proteins but also in chromatin-bound proteins The Mcm proteins bound to chromatin probably function in DNA replication,...

Ngày tải lên: 20/02/2014, 23:20

... successfully applied for data mining The main strength of GeneTrail was that it offered answers to individual biological questions with its feature of incorporation of individually defined categories (such ... transcriptional profiling have strongly advanced our understanding of complex networks of gene interactions in physiology and development The most common integrative approach for measuring gene expression ... predominantly containing bottom-ranked genes (see example in Additional file 2, Figure S2B) The enrichment of a category did not imply a differential expression of all genes of this category The expression...

Ngày tải lên: 11/08/2014, 11:20

... horizontal line indicated in panel (B) (D) YZ view of the anticlinal wall of the region boxed in red in panel (A) (E) Fluorescent intensity values scanned along the horizontal line indicated in panel ... control of the microtubule array that could include modulation of the KATANIN-dependent severing of intersecting microtubules [46] and the dynamic remodeling of the inner-most network of cellulose ... periclinal surface in the red-boxed region of cell (G) XZ view of the anticlinal wall of the region boxed in green in panel (F) (H) Fluorescent intensity values scanned along the horizontal line indicated...

Ngày tải lên: 11/08/2014, 11:21

... abundance was similar in wild-type and sco1-1 seedlings (Figure 4) Since the EMS mutation in sco1-1 converts a glycine contained within the GTP-binding domain to an arginine, transcription was ... sectoring phenotype varied from seedling to seedling In the absence of supplemental sugar, over 80% of the sco1-1 seedlings failed to produce true leaves and died (Table 1) The mutant seedlings ... activity of SCO1 in sco1-1 and sco1-4 Bank:X00415], including conservation of glycine 132, which was changed to an arginine in the sco1-1 mutant During the elongation phase of plastid protein biosynthesis,...

Ngày tải lên: 12/08/2014, 05:20

... cells cultured with d of TGFa and d of KGF Similar findings were observed in ATII cells cultured in KGF (K) alone, in d of K and d of KD (data not shown) or in d of KIA and d of KIAD (Additional ... production of mature SP-B Napsin A and pepsinogen C, asparatic proteases, are also involved in the processing of proSP-B [53,63] We found no changes in the protein levels of napsin A or pepsinogen ... its site of synthesis to the lamellar bodies [53,54] Cathepsin H is one of the cysteine proteases involved in the processing of proSP-B and proSP-C The first N-terminal processing step of proSP-C...

Ngày tải lên: 12/08/2014, 11:23

... of proteins in seeds, plant suspension cells, phyllosecretion and by rhizosecretion from engineered plant roots For production of recombinant proteins in seeds, such as expression of avidin in ... 2.3.1 Expression of recombinant proteins in cell suspension cultures The utilization of plant cells for the production of recombinant compounds of commercial interest has gained increasing attention ... recombinant proteins Targeting signals retain recombinant proteins within distinct compartments of 28 the cells, preserve integrity and protect them from proteolytic degradation leading to increased...

Ngày tải lên: 12/09/2015, 11:08

... and in plant cells using kanamycin (50 mg/ml) Plasmid pASVGUS, a derivative of pASV82 (8.2 kb) containing the GUS expression cassette, was constructed by inserting the 3-kb HindIII fragment of ... coat-protein (CP), and proteins that participate in the control of replication and gene expression The DNA B component encodes proteins required for nuclear trafficking and cellto-cell movement of the ... The recombinant plasmids described in the Results are indicated in boxes The unique HindIII in pASV82 can be used to clone expression cassettes with the gene of interest Optimization of electroporation...

Ngày tải lên: 16/09/2015, 17:13

... implicated in the response of maize to ABA [16] A notable feature of the signaling elements influenced by AtNHX1 is the number of transcripts encoding calciumbinding proteins, including of the transcripts ... included signaling elements, DNA binding elements, components of the protein processing and trafficking machinery, and enzymes involved with metabolic and energy balance of the cell Details of ... meaningful expression levels [15] AtNHX1 influences salt-responsive transcripts encoding signaling elements, including several putative calciumbinding proteins Thirteen salt-responsive signaling-associated...

Ngày tải lên: 12/08/2014, 05:20

... function of most of the J-domain proteins remains to be elucidated The function of several known J-domain proteins will be discussed in detail in section 1.5 29 1.5 Function of J-domain proteins during ... J-domain proteins in the Arabidopsis genome (Rajan and D'Silva, 2009) These J-domain proteins are classified into four distinct groups Type I J-domain proteins have all of the four domains organized ... organized in a sequential order as described in section 1.4.1.1, while Type II J-domain proteins not possess the zinc finger domain Type III J-domain proteins contain only the J-domain In contrast...

Ngày tải lên: 11/09/2015, 10:02

... Studies of ectopic expression of WUS in pericycle 44 4.2.5 Studies of ectopic expression of WUS in endodermis 47 4.3 Discussion Chapter Studies of ectopic expression of WUS in inducible lines 5.1 ... Studies of ectopic expression of WUS in non-inducible lines 34 4.1 The GAL4-GFP enhancer trap lines 34 4.2 Generation and studies of the non-inducible lines 35 4.2.1 Studies of ectopic expression of ... Studies of ectopic expression of WUS in endodermis 61 5.2.4 Studies of ectopic expression of WUS in cortex 78 5.2.5 Studies of ectopic expression of WUS in quiescent centre 80 5.3 Studies of changes...

Ngày tải lên: 26/09/2015, 09:47

... metabolic engineering of freezing tolerance in plants should include such an analysis of the dynamics of metabolism to gain comprehensive information about the effects of individual overexpression ... dynamic modelling of cold-induced reprogramming of central carbohydrate metabolism in A thaliana Performing experiments with two accessions of different origin, i.e Rschew (Rsch), originating from ... of cold acclimation in A thaliana A D B E C F Fig Maximum activities of enzymes in central carbohydrate metabolism during cold exposure (A–C) Vmax values of three invertase isoforms: vInv, nInv...

Ngày tải lên: 14/02/2014, 22:20

... Reconstitution of the branch-point A constant ux of Phser was obtained with puried homoserine kinase in the presence of saturating concentrations of ATP and homoserine Two different coupling systems ... used in order to measure threonine and cystathionine ux Threonine ux was measured using puried threonine deaminase and commercial lactate dehydrogenase Threonine deaminase transforms threonine into ... two features explain the response of the system to the modications of the ux of Phser as indicated in Fig 3F Consequences of CGS ping-pong kinetic mechanism on the branch-point kinetic properties...

Ngày tải lên: 20/02/2014, 02:21

... ERSE-like Xbp1 binding-like P-UPRE ERSE-like ERSE-like ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like – Xbp1 binding-like ... purification of the recombinant luminal domain of At SEL-1L BL21(DE3) cells were transformed with pET-30/At SEL-1L The expression of the putative luminal domain of At SEL-1L was induced by the addition of ... equilibrated with the urea-binding buffer After washing the cartridge with the urea-binding buffer, the luminal domain of At SEL-1L was eluted with the urea-binding buffer containing m imidazole, and...

Ngày tải lên: 07/03/2014, 21:20

... sugarbinding pocket of At1g21360 very rich in arginines Fourthly, the residue corresponding to Val209 in human GLTP corresponds to Ser202 in AtGLTP1, a methionine in At1g21360 and a proline in At3g21260 ... Close-up of the sugar-binding pocket in the crystal structure of human GLTP with bound GalCer in yellow Binding residues are shown in grey (C–E) Sugar-binding pocket in the structural models of AtGLTP1 ... At1g21360 promoter, staining was detected in (J, K) roots of 2-day-old seedlings, (L) root hairs of 6-day-old seedlings, (M) root hairs of 2-week-old seedlings (N) lateral roots of 1-week-old plants...

Ngày tải lên: 23/03/2014, 07:20

... difference Analysis of two-dimensional crystals The study of negatively stained intact inside-out paired membranes can give interesting insight into the packing and ordering of PSII supercomplexes, ... be of coincidence, but we would like to point out that in the latter type of megacomplex, the packing of the supercomplex is very similar to the packing in the crystals, as is shown in the fitting ... appearance of the first complete genomic sequence of a plant, Arabidopsis [27], new perspectives for further investigation of all individual photosynthetic proteins, becomes available Analysis of the Arabidopsis...

Ngày tải lên: 24/03/2014, 04:21

... contents in leaves of Arabidopsis plants cultured with mM nitrate in air (F) Ornithine, citrulline and arginine contents in leaves of Arabidopsis plants cultured with mM ammonium in air (G) Ornithine, ... 5.8-fold increase in arginine relative to the wild-type plants in air, whereas in high CO2, arginine remained at a wild-type level, indicating that the high level of photorespiratory ammonium was in ... accumulation of arginine (Fig 3) Arginine synthesis involves ornithine formation from glutamate [6] Carbamoylation of the ornithine d-amino group with CP leads to the formation of citrulline as a...

Ngày tải lên: 30/03/2014, 01:20

... located in the coding region of these transcripts, the resulting protein isoforms will in many cases differ by an indel (only these type of sequence variations were considered in this study) In order ... the an initial peptide population This initial peptide population consisted of all peptides that theoretically could be obtained through digestion of the proteins (including isoforms resulting from ... digestion of the parent protein As a result, each experimental peptide set was in fact a subset of an initial population that was generated by performing an in silico digestion of all annotated proteins...

Ngày tải lên: 11/08/2014, 11:20