transformation of bacterial with plasmid

... fragment into other plasmid vectors 43 2.3.4 Transformation of bacterial with plasmid 43 2.3.4.1 Preparation of DH5α E coli competent cells 43 2.3.4.2 Plasmid transformation of E coli cells ... Figure 1-11 Organization of the assembly unit in the COPII cage A Comparison of the molecular model of the Sec13/31 assembly unit with the asymmetric unit of the cryo-EM map of the mammalian COPII ... cages of variable curvature (Figure 1-11), which would be essential for the accommodation of a variety of transport cargos 28 Figure 1-10 30A° resolution map of the Sec13/Sec31 cage a, Views of...

Ngày tải lên: 14/09/2015, 12:11

... Electrophoretic Method pp 53 – 109 37 11 Department of health and ageing office of the gene technology regulator, 2005 The biology and ecology of rice (O sativa) in Australia 12 Hurkman and Tanaka, ... edition, Manila, Philippine, IRRI p 253 14 Laemmli U K., 1970 Cleavage of structural proteins during the asembly of the head of bacterio- phage T4 Nature London 227, 680 – 685 15 Nazrul Islam, Lonsdale ... James A Cuikema, Frank F White, Jan E Leach, 1995 Department of Plant Pathology, Throckmorton Hall (S.A.Y., F.F.W., J.E.L.), and Division of Biological Sciences (J.A.G.), Kansas State University,...

Ngày tải lên: 06/11/2012, 09:49

... D McDaniel (2004), A Practical Manual for Understanding the Shell Structure of Broiler Hatching Eggs and Measurement of their Quality, Mississipi State University, p 3-5 ... Trans ASEA, pp 31 Husain A., Chen C.S., Clayton J T, Whitney L.F (1972), Mathematical Simulation of Mass and Heat Transfer in High Moisture Foods, Trans ASAE, pp 55-59 224 Husain A., Chen C.S., ... hợp hệ thống điều khiển cho toàn hệ thống Tài liệu tham khảo Crank J., (1975), The Mathematics of Diffusion, Oxford University Press, Bala, B K (1998), Solar drying systems: Simulations and optimization,...

Ngày tải lên: 28/08/2013, 08:31

... number of research papers of some International Journals Dr Das is currently acting as the honorary member of editorial board of Indian Journal of Science and Technology and as Referee of AMSE ... the rate of heat transfer have been discussed with the help of Figures 1-5 and Tables 12, respectively 4.1 Velocity field The velocity of the flow field is found to change substantially with the ... values of Re with Pr = 0.71, Kp = 1, α = 0.2, Ec = 0.01, ε = 0.2 4.3 Skin friction The variations in the value of x- and z-components of skin friction at the wall for different values of α and...

Ngày tải lên: 05/09/2013, 14:58

... 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine - As (POPC-As) As As Case As links with Phosphate As Case As links with Choline Cơ chế loại thải arsenic qua màng tế bào Cơ chế bơm arsenic qua màng ... khác Relative fluorescent intensity [a.u] Arsenic hóa lỏng màng tế bào 0.35 0.30 With arsenic 0.25 0.20 0.15 0.10 Without arsenic (control) 0.05 0.00 100 200 300 Time-course (min) Sự hấp thu calcein ... 403–448 Các cấu trúc hóa học chất mang hòa tan màng …Arsenic liên kết với tất Ivan Stanish, Journal of Membrane Science 192 (2001) 99–113 Arsenoliposomes tác động mạnh lên tế bào ung thư, gây ảnh...

Ngày tải lên: 17/12/2013, 12:23

... Eller Center, The University of Arizona Of ce of Technology Transfer, The University of Arizona NBER, The University of Arizona Academy of Entrepreneurship, University of Illinois, Urbana-Champagne ... impact of the university’s involvement with science parks on various aspects of the academic mission of the university Their results suggest that the existence of a formal relationship with a ... determinants of the formation of university spin-off companies within the university’s research park and report that university spin-off companies constitute a greater proportion of the companies...

Ngày tải lên: 19/02/2014, 05:20

... form of miscellaneous taxes and assorted fees The process of extraction often turned predatory because of a lack of standardized, legally enforceable procedures limiting the demands of officials ... of the central state, of cials of the local states, and various parts of society On the burden issue, because of their concern with stability, the central authorities sided with the peasants, leading ... toward the end of the dynastic rule and the early era of the Republic First, the weakening of central power during the decline of the Qing, the period of warlordism, and the era of Nationalist...

Ngày tải lên: 19/02/2014, 08:20

... per cent of profits from the export of computer software or the provision of technical services related to software Japan allows a special deduction of the income derived from the export of certain ... provision of services Payment for the transfer may take the form of a sale price in money (or in the form of an item of property, such as the shares of a corporation), of some type of recurring ... the context of technology transfer, one of the most important functions of tax treaties is to reduce the rates of withholding tax that are imposed by the importing country on payments of royalties,...

Ngày tải lên: 19/02/2014, 08:20

... attributed to bH of Ala12 and NH of Val15, and aH of Pro13 and NH of Val15 Ó FEBS 2004 Structural study of internal fusion peptide of ASLV (Eur J Biochem 271) 4733 Conformational alteration of IFP–wt ... to 190 nm at a scanning rate of 50 nmÆmin)1 with a time constant of s, step resolution of 0.1 nm, and bandwidth of nm The final spectra were taken from the average of five scans The VARSELEC program ... labelled with TAMRA following the standard Fmocamino acid coupling protocol with a coupling time of 10 h Labelling with NBD was achieved according the procedure of Rapaport and Shai [13] with modifications...

Ngày tải lên: 19/02/2014, 16:20

... in terms of translation functions ~- : and v -1 between subsets of the f-structure and UDRS formalisms Below we give a relational formulation of the correspondence ~ with a treatment of simple ... [PRED OFT][~] [PREDOE.NE ['4] ] a { like(n4), SUBJ(n4,n2), Hans(n2), XC0MP(n4,nl), cook(nl), SUBJ(nl ,n2), ADJN(nl,n3), often(n3) } LADJN {[paED OFT~.]Sl)J IT :J x[~] Hans(x~]) I ADJN(nl,n3), oft(n3), ... koehen(x~) I 344 { oft(E) x[] Hons()l lT:lHans(x~) I lT :I x[] (9) (ADJN(E,X) } { ADJN(E,X) ] ]- { often(E) } ! (8a) corresponds to only one of the English translations, namely (6d), of (6a) As...

Ngày tải lên: 20/02/2014, 18:20

... of the S mansoni EST database with the complete sequence of Tc52 revealed a clone (EST AI975843) with around 25% sequence identity with the GST-like domain of the T cruzi protein A fragment of ... incubated in ice with gentle agitation After 30 the supernatant was recovered by centrifugation The resin was washed four times with 250 lL of 50 mM Hepes, pH 8.0, and eluted by adding 10 lL of the same ... GSH, 50 mM phosphate buffer, 0.5 units of glutathione reductase and an aliquot of the protein solution The reaction was initiated by the addition of mM of HEDS at 30 °C and followed by 340 nm...

Ngày tải lên: 21/02/2014, 01:21

... 85% of the loading concentration) has an s-value of 3.1 ± 0.2 S, and two minor species ( 5% each) have s-values of 1.9 and S, respectively These results agree well with the distribution of species ... sequence of CTC) These fragments show that one of the two juxtaposed Cys residues at positions 120 and 121 forms a disulfide with Cys148 and the other forms a disulfide with Cys189 The juxtaposition of ... proteolysis of CTC (Fig 3) gives experimental support for the existence of a folded entity that agrees well with the proposed B Fig Thermal unfolding of CTC in the absence and presence of phospholipid...

Ngày tải lên: 07/03/2014, 05:20

... amount of 105–110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top of the gels The amount of aggregate present at the top of the Western blot of the ... Coomassie blue stain of 30-lL samples of fractions (B) Immunoblotting with anticaveolin Ig (C) Immunoblotting with anti-NHE1 (HA-tag) monoclonal antibody (D) Results of re-extraction of membrane fractions ... varying conditions (A) Effect of varying concentrations of 2-mercaptoethanol on aggregation of the Na+/H+ exchanger Samples were incubated with the indicated concentration of 2-mercaptoethanol at either...

Ngày tải lên: 08/03/2014, 16:20

... CdSe nanoparticles are consistent with the wurtzite structure of CdSe with measured lattice constants of a = 6.1 Å (this can be compared to the lattice constants of a = 6,077 Å from JCPDS file No ... agreement with the XRD Fig UV–vis absorption spectra of CdSe QDs with patterns discussed earlier and the presence of good various crystalline times surface states on the QDs Table The parameters of ... where E g(a) is the effective band gap of QDs with radius of a, the band gap E g, Bohr exciton radius aB and Bohr exciton energy R * are the specific parameters of bulk material From absorption y...

Ngày tải lên: 14/03/2014, 13:20

... of the conformational changes of the T domain within CT Mutant CTW206 ⁄ 281, in which the Trp residues of the T domain were replaced by Phe, allowed monitoring of the conformational changes of ... spectra of C (Fig 2C, red curve) appeared to be modified, with a loss of signal at 222 nm This suggested some loss of a-helical content, in agreement with previous observations [20] The spectra of ... compared with that of C Altogether, the results indicated that, within CT, the native to MG transition of the T domain was similar to that of the isolated T, whereas the native to MG transition of...

Ngày tải lên: 14/03/2014, 22:20

... example of suboption negotiation In this example, the client wants to negotiate the type of the terminal 26.13 Figure 26.5 Example of suboption negotiation 26.14 26-2 ELECTRONIC MAIL One of the ... WAN, we need two UAs, two pairs of MTAs and a pair of MAAs This is the most common situation today 26.24 Figure 26.11 Services of user agent 26.25 Note Some examples of command-driven user agents ... is electronic mail (e-mail) The designers of the Internet probably never imagined the popularity of this application program Its architecture consists of several components that we discuss in...

Ngày tải lên: 15/03/2014, 12:20

... contents of soil metals concerned, particularly of soil Cd, had shown a considerable amount of pollution ŽNan and Zhao, 2000 The sampling region consisted of three areas according to the types of irrigation ... L with repeated applications of sewage sludge J Agric Food Chem 1984;32: 155᎐163 Kisku GC, Barman SC, Bhargava SK Contamination of soil and plants with potentially toxic elements irrigated with ... types of irrigation water, i.e one area irrigated with Yellow River water ŽYA., one with the mixture of wastewater and Yellow River water ŽMA and one with wastewater ŽWA Soil and entire crop samples...

Ngày tải lên: 15/03/2014, 23:22