transfer and detection of proteins on membrane supports
Báo cáo hóa học: " Sensitive and molecular size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy" pptx
... function of SOD1 concentration absorption spectra for the SOD1 concentration-dependent response When the gold nanoisland substrate is exposed to an SOD1 solution, the peak position is red and shifted ... higher concentrations, the binding of SOD1 became saturated, leading to a nonlinear response and a plateau for concentrations in excess of 50 μg/ml The reason for the non-linear response at higher ... separation on the surface would form a binary mixture due to ω-functional group interaction [28-31] The mole fractions of these molecules on the Page of gold nanoisland in the fabrication of the...
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Báo cáo sinh học: "A microsatellite-based analysis for the detection of selection on BTA1 and BTA20 in northern Eurasian cattle (Bos taurus) populations" doc
... deviation in one pairwise population comparison (see Table 2) Therefore, it can be considered that the approach was quite robust and conservative in the detection of the effects of Page 11 of 14 ... signatures of homogenizing selection among populations and/ or balancing selection within populations All three methods identified loci SOD1 and AGLA17 as good candidates for selection on the polled ... domestication and not among the domestic breeds Unfortunately, due to the lack of information on the mutation and recombination rates, as well as the effective population size for these data, estimation...
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Báo cáo sinh học: "Combining two Meishan F2 crosses improves the detection of QTL on pig chromosomes 2, 4 and 6" ppt
... the detection of QTL on pig chromosomes 2, and Genetics Selection Evolution 2010 42:42 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission ... microsatellite SW240 on SSC2, between microsatellites S0301 and S0214 on SSC4 and along two regions on SSC6 (between SW2535 and SW1057 and between S0059 and SW607) Initially, French and Dutch pedigree ... to the detection of common QTL (BFT on SSC2 or IMF on SSC4) In some cases, the QTL detected with the combined pedigree had only been described in one of the two designs like birth weight on SSC4...
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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc
... – – Heptamer Monomer Monomer Monomer Monomer Monomer Dimer Monomer Dimer Dimer Dimer Monomer Dimer Tetramer Monomer Monomer – 1F37 – 1VJW 1ZRP 1WD0 – 1G6P 1HTA – 1B8Z – 1CZ3 1IOF 1X1P 1TMY a ... Comparisons of the thermodynamics and kinetics of the folding of proteins from mesophilic and thermophilic ⁄ hyperthermophilic organisms can provide an insight into the mechanisms of stabilization ... [26] Nonheme iron proteins Iron-sulfur (Fe–S) clusters are common cofactors in nature that facilitate electron transport in many proteins (e.g ferredoxins; Fds) [31] Aquifex aeolicus is the only...
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Báo cáo khoa học: Emerging tools for real-time label-free detection of interactions on functional protein microarrays ppt
... cantilever’s resonant vibrational frequency, which is sensed electronically h, Angle of the reflected light determined a Kd of 15 pm, and they demonstrated detection of 50 pm Taq in a solution containing ... magnitude of the change in the local index of refraction caused by binding is a function of the mass and conformation of both the query molecule and the immobilized protein, the number of query ... development of functional protein microarrays and promising technologies for the real-time label-free detection and characterization of protein interactions that may provide higher resolution functional...
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RCUK Policy and Code of Conduct on the Governance of Good Research Conduct pptx
... creation of false data or other aspects of research, including documentation and par ticipant consent Falsification This includes the inappropriate manipulation and/ or selection of data, imagery and/ or ... only in the conduct of research but in publication of materials, preparation of conference papers, etc and the conduct of peer review Ethical approval procedures • ROs should have clear and full ... RCUK Code of Conduct and those of other relevant professional bodies; • be drawn to the attention of all staff on appointment; • be easily available at all times in guidance manuals and on websites...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... Assessment of contribution of each amino acid residue of E51 sequence to substrate recognition Alanine substitution mutants in the E51 sequence were produced as GST(QN) fusion proteins, and then ... in the presence of CaCl2 As a negative control, incubation with FITC– pepE51QN and co-presence of EDTA in the reaction of pepE51 were carried out under the same reaction condition Bar represents ... Thus, TGase and TGase appeared active in distinct regions of the hair follicle cells Discussion During differentiation of keratinocytes and hair formation, isopeptide cross-linking of several...
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Báo cáo khoa học: Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin potx
... contribution of contact and non-contact residues of antibody in the affinity of binding to antigen The interaction of mutant D1.3 antibodies with lysozyme J Mol Biol 234, 958–964 Gomez I, Miranda-Rios ... reached equilibrium Oligomerization of Cry1Aa toxin and detection of oligomerized toxin For the construction of oligomer, 20 lL of a 10 or 100 nm toxin solution were incubated for h at 37 °C ... region is located on or around (365)LYRRIILG(372), R311, N376, G442 and Y445 (Figs 2, and 5), and therefore loops 1, and (especially loop 2) are good candidate regions for the introduction of...
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Báo cáo sinh học: "Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function" potx
... R, McFarland E, Fenton T, for the Pediatric ACTG Cryopreservation Working Group: Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic ... effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function Journal of Translational Medicine 2011 9:26 Page 13 of ... proportion of cells that are apoptotic or necrotic (as defined by Annexin V and 7AAD staining); i = the storage conditions of the whole blood specimen; and c = the storage condition of the control...
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Báo cáo lâm nghiệp: "Improving RBS estimates – effects of the auxiliary variable, stratification of the crown, and deletion of segments on the precision of estimate" pps
... al 2001) During this period, there have been only smaller conceptional contributions, such as the introduction of the terms conditional and unconditional probabilities (Valentine et al 1984) These ... stratification The estimate from RBS samples depends both on the cumulated value of the target variable beyond a certain stage or segment and the conditional (and concomitantly, on the unconditional) ... relationship between the coefficients of variation of the branch biomass and the exponent of the diameter was observed for (24 [19.9]%) o d m Unconditional probability (Qr) Fig The deletion of segments...
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báo cáo khoa học: "Co-expression and impact of prostate specific membrane antigen and prostate specific antigen in prostatic pathologies" ppt
... Authors’ contributions RO contributed to the conception and design of the study; RO and ABJ contributed to data analysis, interpretation and to manuscript writing; ABJ, YB, SS, AB, NBR, LO, YN and ... Page of secretory epithelial cells of prostate gland, both PSMA and PSA transcriptions are androgen-dependent [39,40] The emergence of androgen-insensitive tumor cells arising as a consequence of ... BPH and PC) by immunohistochemistry to evaluate the relationship between the co-expression of prostate- associated Page of antigens (PSMA and PSA) and the degree of vascularization (intensity of...
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cáo khoa học: " Study protocol: Addressing evidence and context to facilitate transfer and uptake of consultation recording use in oncology: A knowledge translation implementation study" potx
... understanding of this intervention, the provision of recordings of key consultations may benefit most adults with cancer, and practitioners should therefore consider offering consultation recordings ... copy of the Cochrane Collaboration systematic review of consultation-recording use, copies of the publications of the consultation-recording studies conducted by the research team, and a copy of ... the use of consultation recordings by oncology professionals Lack of exposure to the benefits of consultation recordings may result in clinicians who believe there is a lack of positive, consensus...
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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
... number of unique sequences Ka/Ks ratio = ratio between non-synonymous and synonymous mutations Range substitutions = minimum and maximum number of amino acid substitutions in pair wise comparisons ... between Glu42 and Ile56 and contains only a single amino acid variation among all 80 isoforms A phosphate-binding loop with the sequence motive GxGGxGx characterizes this region Additional conserved ... that a combination of recent gene duplications and purifying selection could also explain why tandem gene duplicates appear similar In their model of birth -and- death evolution of genes, new genes...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
... Jacobson S: Detection of virus-specific T cells and CD8+ T-cell epitopes by acquisition of peptide-HLA-GFP complexes: analysis of T-cell phenotype and function in chronic viral infections Nat ... Production and functional capabilities of peptide-β2mRT1.Al fusion proteins To establish an activation system of Tax-specific CTLs using SCTs of rat MHC-I (RT1.Al), we have constructed expression ... HTLV-I-infected rat and that the detection of the epitopespecific CTLs by SCT-EGFP system was comparable to the measurement of peptide-induced IFN-γ production Thus, the activation and detection system...
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Báo cáo y học: "The effect of different volumes and temperatures of saline on the bladder pressure measurement in critically ill patients" potx
... design and coordination, and performed the measurements SA participated in the study design and coordination, and performed the measurements CC participated in the study design and coordination, and ... Mullins RJ, Brand DM, Crass RA, Trunkey DD: Surveyed opinion of American trauma surgeons on Available online http://ccforum.com/content/11/4/R82 37 38 39 40 41 42 the prevention of the abdominal ... 0.05 was considered statistically significant The mean bias (bladder minus gastric pressure), precision (standard deviation of the bias) and limits of agreement were calculated using the Bland-Altman...
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Báo cáo sinh học: " Effects of the number of markers per haplotype and clustering of haplotypes on the accuracy of QTL mapping and prediction of genomic breeding values" pot
... animals of generation 1001 on average had 20 offspring, whereas parents of previous generations on average had two offspring The generation 1002 produced one more generation of 1000 offspring ... investigate the effect of haplotype definition on the accuracy of predicted breeding values for genomic selection and on the precision of QTL mapping, given a moderately dense marker map and http://www.gsejournal.org/content/41/1/11 ... Definition of windows and clustering of related haplotypes Two types of haplotypes were considered: 1) the haplotypes of the first generation of genotyped animals (referred to as base haplotypes), and...
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Báo cáo sinh học: "Analysis of a simulated microarray dataset: Comparison of methods for data normalisation and detection of differential expression (Open Access publication)" pps
... table contains the gene id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS) and ... methods Table contains the gene id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS), ... in recognition of the clear relationship between M and A Only ROSLIN (assessed normalisation by row and 674 M Watson et al Figure MA-plots of slides 1, and These slides are examples of the three...
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Báo cáo y học: " Sequence-based feature prediction and annotation of proteins" ppt
... experimental detection of this type of protein, and similarly, detection of alternative splicing by exon and tiling arrays also contributes large amounts of functional evidence of pleiotropy ... existing GO evidence ontology This development is also very important for the future evolution of function-prediction tools Functional annotation of positional and non-positional features from ... sequence conservation of a long sequence segment” are also very useful tools for predicting function Many Pfam functional domains and alignments are manually constructed by experts and are often among...
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report and proceedings of committee on banking and currency
... the removal of all restrictions on rates of interest The course of the money market in London during the crisis of 1857 sufficiently proved this beyond all question : and the restrictions in force ... charge for interest, to consider the nature of each transaction and the position of the money market On the part of the public, I have not noticed any expression of dissatisfaction when the increased ... mismanagement and imprudence of the Banks in the other States of the Union " " Each circulation, h — To Question 10 —The Bank of British North tions in 1836 with a capital of one million To Question II...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008