transfection lysis and analysis of protein following immunoaffinity column purification
Báo cáo y học: " Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques" pot
... before administration of tenofovir d Mean ± standard deviation of days -14, -6 and before administration of tenofovir e Day before administration of tenofovir b Challenge Page of 13 (page number ... setting of chronic SIV infection Results Effect of tenofovir on SIVmac055 challenge of naïve rhesus macaques To evaluate the dose and replicative capacity of SIVmac055 in the presence of tenofovir, ... was undetectable and remained so throughout a year of follow-up Several blips of viremia occurred, and analyses of both viral RNA and DNA demonstrated persistence of nef deletions and pol sequences...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... measurements of the DNA-content in cells and tissues Within the scope of the tumor diagnostics, objective and valid gradiations of the malignant potential of cells of different tumors and inside of a ... diploid and 24 % aneuploid cells in the TMZ probe G1-phase: The comparison of the amount of aneuploid and diploid cells in the G1 phase shows a ratio of 49 % and 54% The cells in the G1 phase of the ... decreased amount of diploid (15%) but an increased amount of aneuploid cells (49%) The increase of diploid cells to 28% in the G2/M phase and a parallel decrease of the amount of aneuploid cells...
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báo cáo hóa học:" Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist" potx
... greater and was to be documented here by retrospective analysis of phase one data Instrument set-up, MESF calibration and data analysis A Becton Dickenson FACSCalibur instrument using 488 argon and ... precision and reproducibility of the assay The precision was determined both in the in vitro validation phase and through retrospective analysis of in-study data Methods Generation of Alexa 488 ... 5.0 (Verty Software House) Curve fitting and determination of EC50 and IC50 values was performed using Prism 4.0 (Graphpad) when applicable The mean, standard deviation, standard error and % coefficient...
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báo cáo hóa học:" Static platelet adhesion, flow cytometry and serum TXB2 levels for monitoring platelet inhibiting treatment with ASA and clopidogrel in coronary artery disease: a randomised cross-over study" potx
... eppendorf-tubes and stored at -70°C until analysis of TXB2 For patients, blood samples were also drawn into lithium heparin-tubes and K2EDTA-tubes for biochemical analysis at the accredited Department of ... were used for analysis of plasma concentrations of C-reactive protein (CRP), cholesterol, triglycerides, LDL-cholesterol, HDLcholesterol, apolipoprotein-A1 (Apo-A1) and apolipoprotein-B (Apo-B), ... between the control means of visit and and treatment with ASA (A), clopidogrel (C) and the combination of ASA and clopidogrel (A+C) The respective figures show the effect of platelet inhibiting...
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Báo cáo khoa học: " Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules" ppsx
... RTH230A, and RACT53A) Cross comparison of the patterns of reactivity of the mAbs using color FC showed RTH2A and RTH230A; RT21A and RTH21A; and RTH26A, RTH65A, and Ken5; recognize Pan T1, Pan T2, and ... with Pan T1, CD4, and CD8 mAbs and anti-rabbit Ig demonstrated that the LDM is Fig Two color FC analysis of the expression of RTH192A on T and B lymphocytes The level of expression of PanT4 on RTH192A+ ... (198) and subsequent Fig Two color analysis of the expression of LDM detected with RACT20A and MRB120A on basophils, monocytes, and CD4 T lymphocytes mAbs to CD14 and Pan T1 were combined and used...
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Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus" pps
... Evaluation of FCM analysis of GR expression by western blot Analysis of GR repression in (left) K562 and (right) U937 with and without 10-6 M Dex of GR expression by western blot by GR-mAb FCM and western ... Page of 11 (page number not for citation purposes) Arthritis Research & Therapy Vol 11 No Du et al Figure Evaluation of FCM analysis of GR binding by RLBA Analysis of GR binding in (a) K562 and ... μL fixation buffer FITC-Dex-FCM analysis for the binding of GR The protocol for surface staining and erythrocyte lysis were similar to analysis for the expression of GR above Cell pellets were...
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Báo cáo y học: "The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives" pot
... expression of degranulation markers [11-13] Principle of basophil activation test by flow cytometry As flow cytometry is a valuable tool for the analysis of many different cell types and can be ... interlab standardization of this kind of test Lastly, since CRTH2 is also a marker of Th2 cells and eosinophils, it may become a promising tool for flow cytometry, providing a direct overview of cells ... definition of the threshold for positivity (using ROC analysis) since the use of an arbitrary cut-off value is likely not suitable for all allergens The present challenge is to take advantage of the...
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Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps
... assembly and cleaning up of the data, removal of systematic biases and drifts (a process often referred to as 'normalization'), and transformation to a format and scale that is suitable for the following ... suitable for the following analysis steps Here we not deal with the technical aspects of data import and management, and refer the interested reader to the documentation of the software package prada ... light scatter, α and β are the coefficients of the model, and xspecific is the specific fluorescence We compute α and β by robust fit of a linear regression of xtotal on s, and obtain estimates...
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Intake manifold design effect on air fuel mixing and flow for an LPG heavy duty engine
... numerical analysis of flow in two manifold geometries using a CFD code FloWorks is chosen for its capabilities flow analysis for both in gas and liquid simultaneously 3.1 3D geometries model of manifolds ... understanding of the nature of acoustic wave dynamics in the intake system of an internal combustion engine The model has proved in identifying the role of pipe resonance in the intake process and ... Intake manifold effect on air-LPG ratio and SFC Conclusion The main conclusions we can draw from this paper are the following: 1- The analysis of the flow and mixture motion features, during the...
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Clinical Flow Cytometry – Emerging Applications Edited by Ingrid Schmid ppt
... and flavins as metabolic status markers), in DNAs analysis, protein, peroxide production, and intracellular pH, for count of live and dead bacteria and/ or yeasts, and for the discrimination of ... dual-parameter analysis of forward light scatter and red fluorescence signals (FSC-H vs FL3-H) allowed the discrimination between two species of Candida, as Candida lusitaniae and Candida maltosa, ... J.R (2004) Automated flow cytometry analysis of peritoneal dialysis fluid Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European...
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flow cytometry protocols 2d ed - teresa s. hawley
... Instrument vendors write software for the analysis of data acquired on their instruments; independent programmers write software for the analysis of data acquired on any instrument Software packages vary ... knowledge of the wavelength of the illuminating light, knowledge of the wavelength specificities of the filters in front of the instrument’s photodetectors, and knowledge of the absorption and emission ... used in cytometry Uptake and efflux of dyes, drugs, and other reagents by and from bacteria are affected by the structure of the cell wall, and by the presence of pores and pumps that may or may...
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flow cytometry protocols
... pH (260 g/L of distilled water) and a A4 NaCl solution (87.7 g/L of distilled water) For 100 mL of IFA, mix mL of M HEPES, pH 7.4, 10 mL of M NaCl and mL calf serum, and add 85 mL of drstrlled ... also offer software for data analysis These programs provide many ways to examine data; however, there are some very useful standard ways of presenting data that are common to all types of software ... and Rieger, C (1993) Detection of intracellular cytokmes by flow cytometry J Immunol Meth 159, 197-207 Sander, B., Andersson, J., and Andersson, U (1991) Assessment of cytokines by the immunofluorescence...
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hughes t. groove and flow - six analytical essays on the music of stevie wonder (диссертация)
... Seales, and Larry Starr of the University of Washington, John Covach of the University of North Carolina, Walter Everett of the University of Michigan, Brian Robison of MIT, Mark Spicer of CUNY, and ... Harmony and Bass Line of the Primary Groove of “Higher Ground” 191 21 The Harmony and Bass Line of the Refrain of “Higher Ground” 192 22 The Harmony and Bass Line of the Chorus of “Higher ... musical processes and accounting for some of the unusual power and life of this music TABLE OF CONTENTS Page List of Musical Examples ii List of Figures v List of Tables ...
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Báo cáo hóa học: "Exhaustive expansion: A novel technique for analyzing complex data generated by higherorder polychromatic flow cytometry experiments" pot
... here, our analysis provided the foundation for a refined understanding of complex phenotypes, and allowed for the development of new hypotheses pertaining to the identification and recovery of potentially ... observations that of patients show CCR7+CD57peaks at LTM (Figure 4C), and that of the sets in Figure are subsets of the CCR7+CD57- (+.- ) phenotype Porcine Stem Cell Study Screening of Thousands of Subpopulations ... based on coefficients of variation and long term memory peaks in the melanoma vaccine study, and separation between test and control cohorts in the wound healing study Analysis of data from multiparameter...
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CLINICAL FLOW CYTOMETRY – EMERGING APPLICATIONS pot
... and flavins as metabolic status markers), in DNAs analysis, protein, peroxide production, and intracellular pH, for count of live and dead bacteria and/ or yeasts, and for the discrimination of ... dual-parameter analysis of forward light scatter and red fluorescence signals (FSC-H vs FL3-H) allowed the discrimination between two species of Candida, as Candida lusitaniae and Candida maltosa, ... J.R (2004) Automated flow cytometry analysis of peritoneal dialysis fluid Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European...
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Báo cáo y học: " Diagnostic value of anti-human citrullinated fibrinogen ELISA and comparison with four other anti-citrullinated protein assays" potx
... The cut-off defined for anti-pepA and anti-pepB antibodies corresponds with a specificity of 100% and 99.3% and a sensitivity of 63.6% and 54.2%, respectively [14] Detection of anti-CCP1 and anti-CCP2 ... cluster analysis of variables was performed with the results of RF and five ACPA assays: anti-CCP1, anti-CCP2, anti-pepA, anti-pepB and AhFibA (Figure 3) Table Sensitivities and specificities of AhFibA ... citrullinated peptide This analysis identified the ACPAs apart from the RF Within ACPAs, we identified different clusters: a cluster of pepA, pepB and anti-CCP1, and a cluster of AhFibA and anti-CCP2 Discussion...
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Báo cáo khoa học: "Cutaneous vascular reactivity and flow motion response to vasopressin in advanced vasodilatory shock and severe postoperative multiple organ dysfunction syndrome" pot
... analysis, the Doppler signal tracing was divided into nine blocks of 20 seconds each before induction of ischaemia and during reperfusion measurements (Figure 1) Fast Fourier transformation analysis ... carried out bedside measurements and documentation, performed the statistical analysis, and helped to draft the manuscript KHS participated in the design of the study and performed the calculations ... in the study design and its coordination, and helped to draft the manuscript HU performed the statistical analysis and helped to interpret the data WP conceived the study, and helped to carry...
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Báo cáo y học: "Differential response of human basophil activation markers: a multi-parameter flow cytometry approach" pot
... plasma and the majority of platelets and of reducing the impact of the great variability of individual responsiveness [33] This may be particularly important for in vitro testing of agonists and ... and histograms of CD63 and CD203c expression in response to fMLP Dot plots and histograms of CD63 and CD203c expression in response to fMLP Basophils were incubated in the absence (A,B,E,G) and ... level of CD63 (Figure 3A) and of CD203c (Figure 3B) and their respective fluorescence distribution (Figure 3E and 3G) were comparable to those of the resting cells previously described Following...
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Influence of pipe length and flow rate on nano particle deposition in laminar circular pipe flows
... different lengths of 1, and cm and a mean velocity of m/s Figures 8-10 display the deposition efficiency for a pipe with the length of 5cm and different mean velocity of 0.5, and m/s in compare ... department of Petroleum and gas Engineering, University of Salford, United Kingdom His main research interests include biofuels, emission reduction, thermoeconomic and exergy analysis of energy ... with the length of 1cm and a mean velocity of m/s Figure Comparison of the deposition efficiency for the 1cm long pipe and a mean velocity of 1m/s between the present result and the analytical...
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