transduction cell cycle arrest and apoptosis

... Perillyl alcohol and perillic acid induced cell cycle arrest and apoptosis in non-small cell cancer cells Cancer Lett 2007, 257:216-222 13 Thornberry NA: Caspases: Key mediators of apoptosis Chem ... (Figure 3) and camptothecin and cis-platin used as standards (Figure 4) Apoptosis recorded in untreated control MOLT-4 and HL-60 cells were 3.61% and 2.54% respectively In MOLT-4, total apoptosis ... activity interfered with S and G2/M phases of cell cycle of MOLT-4 cells As a preparatory step towards cell division, a cell duplicates its DNA in S phase of cell cycle Thus, interference of...

Ngày tải lên: 10/08/2014, 10:20

... AGE-BSAmediated cell cycle arrest in FLS The observation that AGEBSA induces necrosis and late apoptosis but not early apoptosis in FLS agrees with these findings because cells arrested in the cell cycle ... in six different FLS cell lines with eight per treatment group Cell cycle analysis and evaluation of cell death For cell cycle analysis FLS were harvested after 4, 8, 16, 24 and 48 hours after ... concentrations of specific AGEs such as CML and pentosidine AGE-BSA is actively incorporated into cultured FLS and induced to cell cycle arrest, decreased cell proliferation and viability, but also increased...

Ngày tải lên: 09/08/2014, 14:22

... proliferation and motility and arrests Rat1/ras cell growth at the G1 phase of the cell cycle Experimental procedures Rat1/ras or Rat1/myr-ras cells was plated in agar at a density of 000 cells per ... growth and on the cell cycle We find that HR12 suppresses anchorage-dependent and independent growth and motility of Rat1/ras (Figs and 2) Furthermore, treatment with HR12 leads to arrest of cell ... Rat1/ras cells, and whether the timing of Ras-processing and pRb phosphorylation are correlated We treated Rat1/ras cells with 20 lM HR12 in growth medium and lysed the treated and untreated cells...

Ngày tải lên: 31/03/2014, 01:20

... Figure of Control1 cell growth and viability in HL-60 cells by LA Control of cell growth and viability in HL-60 cells by LA (A) Cells were treated with 0, 1, 2.5 and mM LA and the cell numbers were ... cell cycle phase distribution and the expression of various cell cycle regulatory proteins in HL-60 cells Figure Effects of LA on cell cycle phase distribution and the expression of various cell ... in the G1 phase cell population (28.4% in control vs 63.1% and 74% in 2.5 and mM LA treated cells) To further explore the cell cycle arrest by LA in HL-60 cells, specific cell cycle regulatory...

Ngày tải lên: 10/08/2014, 22:20

... cell cycle replication Presently, no data exists on the subcellular localization of CSFV NS2 protein and its effects on cell growth and cell cycle progression The swine endothelial vascular cell ... approximately × 106 cells of the stable cell lines and control cells were trypsinised and collected Following two washes with PBS, cells were resuspended in 70% ethanol and fixed at 4°C for 18 h Cells were ... protein induces cell cycle arrest at S-phase To determine whether the growth inhibition of CSFV NS2-expressing cells was due to the arrest of the cell cycle at a certain phase(s) of cell division,...

Ngày tải lên: 12/08/2014, 04:21

... Materials and Methods Chapter 2: Materials and methods 30 Chapter 2: Materials and Methods Chapter 2: Materials and methods 2.1 Routine cell culture of MN9D and SH-SY5Y cell lines The MN9D cell is ... gene and controls the cell cycle, S-phase, indirectly via a cellular cofactor in the murine cell line (Lee et al 1997) and nm23-M2 activates endogenous c-myc expression both at transcriptional and ... gene will cause MN9D cells to differentiate, inducing neuritogenesis and cell cycle arrest, and 2) siRNA interference of nm23-M2 gene will abolish neurite outgrowth of MN9D cells in differentiation...

Ngày tải lên: 08/11/2015, 17:09

... cancer cell line CKBM is found to inhibit cell proliferation through the induction of apoptosis and G2/M cell cycle arrest To further elucidate the mechanism of CKBM on apoptosis and G2/M cell cycle ... lunasin and lectins in soybean were shown to induce apoptosis in malignant cells [3] Schisandra chinensis is shown to reduce prostate cancer cell growth and induce apoptosis by inhibiting androgen ... with cell cycle analysis, apoptotic study and Western blot analysis The results showed that CKBM is able to induce a G2/M cell cycle arrest and a dose-dependent apoptotic effect in AGS cells...

Ngày tải lên: 02/11/2012, 11:12

... for controlling cell cycle progression (Morgan 1997; Zachariae and Nasmyth 1999) These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with ... cell cycle machinery and the pRB and p53 tumor suppressor pathways The universality of the cell cycle in all eukaryotes strongly suggested that anticancer therapy aimed at regulators of the cell ... S-phase entry and cell cycle progression Overexpression of D- and E-type cyclins and Cdk4 is observed frequently in human tumors (see Sect 5), supporting the notion that these cell cycle regulators...

Ngày tải lên: 25/10/2013, 21:20

... most cell cycle responses While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth factor deprivation and ... in repression of E2F target genes and ultimately in a cell cycle arrest or exit from the cell cycle (see Fig 1) Thus, pRb can be viewed as a molecular cell cycle switch that is either turned ... v-Abl and E2F-1 are known to activate p19ARF expression, leading to a p53-dependent cell cycle arrest or apoptosis, thereby withdrawing cells carrying oncogenic potential from the cell cycle...

Ngày tải lên: 25/10/2013, 21:20

... down-regulated upon cell cycle exit To this effect, HFF cells were deprived of serum for 48 h to induce cell cycle exit Serum was then re-added and samples taken as cells progressed into the cell cycle Total ... proliferating cells [17] Cells cease to proliferate and exit the cell cycle (G0 phase) in response to growth arrest and differentiation signals or when deprived of growth factors The vast majority of cells ... tumor cell lines and compare them to primary cells Cellular lysates were prepared from human foreskin fibroblasts, a primary cell line, and the tumorigenic cell lines Saos, MDAMB231, MCF7, HeLa and...

Ngày tải lên: 07/03/2014, 16:20

... regulation of lipid synthesis and the cell cycle and growth Experimental procedures Cell proliferation and cell- cycle analysis of CHO stable cell lines CHO cell lines, CHO-BP1a and CHO-BP2, expressing ... SREBP-1a on cell growth and cell cycle in lipid deprivation (A) Effects of DLS, and rescue effect of oleate (unsaturated fatty acid) and cholesterol on cell proliferation in cultured cells were ... cellcycle-dependent manner and could thereby regulate the cell cycle and growth It is important to investigate endogenous Fbw7 activity in relation to the cell cycle and lipid availability Our...

Ngày tải lên: 23/03/2014, 07:20

... in vitro wound healing, cell migration and apoptosis, and for DP8 in wound healing, cell migration and apoptosis enhancement (Table 4) DP9 overexpression impaired 2452 cell behavior with regard ... involved in cell ECM interactions and influence apoptosis, but DP8 did not influence adhesion and only DP9 acted as a primary trigger of apoptosis DP8 and DP9 may also have in vivo roles as intracellular ... Table 1, and DP8 was assessed by cell stain (not shown) DP8 and DP9 have been localized to Golgi and endoplasmic reticulum [7,8] Concordantly, in the 293T cells transfected with DP8–GFP and DP9–GFP,...

Ngày tải lên: 23/03/2014, 11:20

... 1-58706-067-1 (alk paper) Cancer cells Regulation Cell cycle Cellular signal transduction Cell transformation Carcinogenesis [DNLM: Cell Transformation, Neoplastic Cell Cycle QZ 202 C39253 2001] I ... Protein and mRNA during the Cell Cycle 126 Subcellular Localization 127 Activity at Cell Cycle Checkpoints 129 Interactions with Cell Cycle Proteins 130 Transcription of Cell ... Cytokine Regulation of Cell Cycle Progression 34 Links Between the Ras/Raf/MEK/ERK Pathway and Cell Cycle Proteins 34 Cytokine Regulation of Apoptosis and Cell Death 34 Apoptotic...

Ngày tải lên: 10/04/2014, 11:13

... Cells Arresting in S Phase of Cell Cycle Gossypol reportedly induces cell cycle arrest in prostate cancer cells and colon cancer cells [8,9] To determine whether ApoG2 could also induce cell cycle ... to trigger signal transduction, culminating in cell cycle arrest and/ or apoptosis [18,19] However, Van Poznak et al and Zhang et al suggested that gossypol-induced cell cycle arrest is associated ... http://www.translational-medicine.com/content/7/1/74 Figure Arrest of NPC cells at S phase of the cell cycle by treatment with ApoG2 Arrest of NPC cells at S phase of the cell cycle by treatment with ApoG2 Arrest of NPC cells at S phase...

Ngày tải lên: 18/06/2014, 15:20

... Histogram of cell cycle arrest and cell apoptosis Histogram of cell cycle arrest and cell apoptosis Histogram showing the percentages of cells at various phases of cell cycle p3b/EGFP-N1 positive cells ... of cell cycle arrest and cell apoptosis by 3b protein expression Induction of cell cycle arrest and cell apoptosis by 3b protein expression p3b/EGFP-N1 plasmid was transfected into COS-7 cells, ... of cells from G0/G1 phase into S phase, and induced cells toward apoptosis Next, using similar method, we investigated the effects of p3b/EGFP-N1 on cell cycles in 293 and Vero cells In these cells,...

Ngày tải lên: 19/06/2014, 08:20

... Histogram of cell cycle arrest and cell apoptosis Histogram of cell cycle arrest and cell apoptosis Histogram showing the percentages of cells at various phases of cell cycle p3b/EGFP-N1 positive cells ... of cell cycle arrest and cell apoptosis by 3b protein expression Induction of cell cycle arrest and cell apoptosis by 3b protein expression p3b/EGFP-N1 plasmid was transfected into COS-7 cells, ... of cells from G0/G1 phase into S phase, and induced cells toward apoptosis Next, using similar method, we investigated the effects of p3b/EGFP-N1 on cell cycles in 293 and Vero cells In these cells,...

Ngày tải lên: 20/06/2014, 04:20

... Loratadine on In Vitro Cell Cycle Progression HT29 cells treated with loratadine (75 μM) for 24 hours Loratadine was then washed off and cells were irradiated (8 Gy) Cell cycle progression was ... loratadinetreated cells clearly dominated the cell cycle delay and radiation with loratadine did not cause additional cell cycle delays As shown in Figure 5B, the percent of cells in G2 did not ... of cells in G2/M phase of the cell cycle Our data suggest that other parts of the cell cycle are also affected since the percentage of cells in G2/M never increased beyond 38% while radiation and...

Ngày tải lên: 09/08/2014, 10:20

... drugs The induction of cell cycle arrest and apoptosis are common mechanisms proposed for the cytotoxic effects of anticancer-drug extracted from herbal medicine [23] Cell cycle arrest can trigger ... 2/M arrest To determine whether Osthole inhibits the cell cycle progression of A549 cells, the cells were treated with different concentrations of Osthole (0, 50, 100, and 150 μM) for 48 h and ... H: Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells Oncogene 2002, 21:3727-38 32 Emi M, Kim R,...

Ngày tải lên: 10/08/2014, 10:21

... mother cell divides symmetrically with differentiation into two neurons INTRODUCTION 1.1.3 Coordination of cell cycle and cell differentiation In a developmental context, the cell cycle and cell ... 1999), including cell fate regulation (Macaluso et al., 2006), senescence (Funayama and Ishikawa, 2007), and apoptosis (Harbour and Dean, 2000b) Its dual function in cell cycle and cell differentiation ... coordinate cell cycle and cell differentiation, and several experiments have been conducted to test this hypothesis However the elaborate relationship between cell cycle and differentiation in multicellular...

Ngày tải lên: 12/09/2015, 09:28

... of the cell cycle and cancer 1.1.1 The general schema of cell cycle progression: simplifying complexity Cell division is characterized mainly by two consecutive processes, DNA replication and segregation ... of cell cycle progression 1.1.2.1 RB/E2F/DP-mediated transcriptional regulation of cell cycle progression The E2F family of transcription factors integrates cellular signals and coordinates cell ... in DNA replication and cell cycle progression, but also of genes involved in DNA damage repair, apoptosis, cell differentiation and development (Bracken et al., 2004; Dimova and Dyson, 2005) Among...

Ngày tải lên: 13/09/2015, 20:18