transcriptional silencing the nucleolus and yeast aging
Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
Ngày tải lên :
07/03/2014, 16:20
... is boxed, and the GAGA and Adf-1 elements in the DNA are underlined The translation start codon and the main transcription start point are larger and in bold (B) Mutations either in the GAGA or ... under the control of the actin 5C promoter The GAGA factor stimulated at least threefold the activity of the promoter in constructs )397/+86 and )146/+86, but had no effect on the activity of the ... protein (mtSSB), and the catalytic (a) and accessory (b) subunits of the DNA polymerase c (Pol c) [24,25] Interestingly, the expression of the genes encoding mtSSB and Pol c-b is transcriptionally...
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Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency pdf
Ngày tải lên :
16/03/2014, 06:20
... view of the structural similarities between yeast and mammalian enolases and the high degree of sequence conservation, we believe that the effects of these mutations on the structure and function ... The G376E and G157D mutations in yeast enolase S Zhao et al yeast enolase can be overexpressed and purified in quantity [4,5] The basic three-dimensional structure of the monomeric unit is the ... function of yeast enolase will be similar to their effects on human bb-enolase Figure shows the basic fold of yeast enolase, including the location of the active site and glycines 157 and 376 Comi...
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Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt
Ngày tải lên :
16/03/2014, 11:20
... Rp and mCtBP2 in yeast cells (A,D) Yeast AH109 cells were transformed with the combination of plasmids shown The growth of single yeast colonies containing these plasmids are shown in (B) and ... of plasmids shown The growth of single yeast colonies containing these plasmids are shown in (B) and (E) on SD lacking L-histidine and adenine and (C) on SD plus L-histidine and adenine FEBS Journal ... (LRPPRC) protein [26] and the transcriptional coactivator CITED2 [27] UXT is also known as ART-27, a transcriptional coactivator that interacts with the N-terminus of the androgen receptor [28]...
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Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx
Ngày tải lên :
16/03/2014, 18:20
... mitochondria) The reason why the action of the two opposite enzymes, namely the arachidonoyl-CoA synthetase and the arachidonoyl-CoA thioesterase, is needed may be the need to sequester AA from the free ... inhibitors of the acyl-CoA synthetase and the acyl-CoA thioesterase, we have previously postulated the existence of a new pathway for AA release that operates in the regulation of steroid synthesis ... Here we address the question of whether MTE-I and ACS4 are indeed essential for AA release and cholesterol metabolism in steroidogenic cells We show for the first time that silencing the expression...
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Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf
Ngày tải lên :
16/03/2014, 22:20
... times; (b) the 33 and 31 kDa species were found only in small proportions; (c) the 46-kDa band was predominant at short and intermediate times, and (d) after 40 min, the 44 kDa band was the most ... temperatures, e.g 42 °C Therefore, the functionality of the peptide-binding site depends on interactions between the b-sandwich and both the lid subdomain and the ATPase domain of the protein This structural ... DnaJ and GrpE in bacteria, and with Mdj1p and Mge1p in yeast mitochondria, respectively [14–17] Despite the homology, DnaK and mtHsp70 are not interchangeable in bacteria or yeast [18,19] In the...
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Báo cáo Y học: Effect of the disease-causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII pot
Ngày tải lên :
17/03/2014, 17:20
... characterizing the thermal denaturation of Tk-RNase HII and its derivatives The melting temperature (Tm) is the temperature of the mid-point of the thermal denaturation transition The difference in the ... affinity of the protein, the interactions between the protein and substrates ([rA]1 and [rA]4) were analyzed in the absence of the metal cofactor by BIAcore The dissociation constants of the proteins ... system The production levels of these subunits in the cells and the amount of the mutant proteins of Sc-RNase H2* purified from L of culture were not seriously changed regardless of the loci of the...
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Báo cáo khoa học: Expressed as the sole Hsp90 of yeast, the a and b isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90b generates sensitivity to the Hsp90 inhibitor radicicol pdf
Ngày tải lên :
23/03/2014, 07:20
... that of the other three strains (Fig 1A) These yeasts, isogenic but for their Hsp90 gene, might therefore be expected to exhibit similar phenotypes Nevertheless, as the studies below reveal, the ... express, from a plasmid-borne Hsp90 gene, either the native yeast Hsc82, the native yeast Hsp82 or human Hsp90b as their sole, essential Hsp90 [17] For the current study, we constructed in this ... with either Hsp90a or Hsp90b in the yeast (Figs and 4) In contrast, activation of v-src was clearly compromised with Hsp90b rather than Hsp90a present in the cells (Fig 3) Evidently, therefore,...
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Báo cáo khoa học: A modelling approach to quantify dynamic crosstalk between the pheromone and the starvation pathway in baker’s yeast pot
Ngày tải lên :
30/03/2014, 10:20
... about the wiring of the pheromone pathway and the filamentous growth pathway in yeast We concentrated on the main dynamic features and the interconnections between the two pathways and on a limited ... predicted by the model On the one hand, the model predicts that a pheromone stimulus inhibits at least transiently the starvation-induced activation of Kss1 and FREP On the other hand, a starvation ... consisting of the scaffold protein Ste5, the MAPKKK Ste11, the MAPKK Ste7 and the MAPK Fus3, and the phosphorylation and activation of nuclear proteins controlling cell polarity, transcription and progression...
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a means to an end the biological basis of aging and death apr 1999
Ngày tải lên :
11/06/2014, 05:26
... Reznick, and Joan Valentine, among others The length of human life, according to the Greeks, was in the hands of the Fates, or Moerae These three sisters, daughters of the Goddess of the Night, ... senescence and aging (gerontology) and a medical specialty dealing with the problems of the aged (geriatrics) were not formed until the 1940s As with any new field, the early years of the study of aging ... hormones produced in the pituitary gland of the brain, and the pattern of synthesis and release of these hormones changes in an age-dependent manner In virtually every species, the degenerative changes...
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Báo cáo sinh học: "Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression" ppt
Ngày tải lên :
06/08/2014, 18:20
... dividing and then proliferate and get progressively smaller [22] Why yeasts and Schwann cells coordinate cell growth and cell-cycle progression so differently? The lifestyles of yeasts and animal ... than small cells of the same type and at the same point of the cell cycle It thus seems likely that most mammalian cells grow linearly, independent of their size, and that they therefore not require ... where the sizes of two, unequally sized, hypothetical daughter cells are followed through several cell cycles If the cells and their progeny grow and progress through the cell cycle at the same...
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Common ground in the transcriptional profiles of wounds and tumors pps
Ngày tải lên :
09/08/2014, 20:20
... revealed that the more the tumor signature resembles the fibroblast core serum response, the more likely the tumor cells are to metastasize, and thus the worse the prognosis is for the patient ... epithelia, and hence provide a link between the extent to which the epithelial-mesenchymal transition has occurred and the fibroblast core serum response Thus it would be interesting to compare the ... vast growth in the understanding of the signaling events that control the wound-repair process Given the similarities between wound repair and tumorigenesis, novel findings in the wound-repair...
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Báo cáo y học: "Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study" ppt
Ngày tải lên :
14/08/2014, 07:21
... billion years ago [5], and many gene sequences are as distantly related between the two yeasts as to their human homologs A comparison between budding and fission yeast can therefore provide valuable ... conditions this also requires the action of the ferric/cupric reductases Fre1p and Fre2p [7,8] Regulation of the copper uptake system is mediated at the transcriptional level by the copper-sensing regulator ... on the basis of sequence similarity To determine the extent to which the S pombe homologs are similarly controlled at the transcriptional level as their S cerevisiae counterparts, we compared their...
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The effects of the histones and histone interacting partners on transcriptional regulation
Ngày tải lên :
12/09/2015, 08:16
... binding on one hand helps to recruit TFIIB to the activated promoter, on the other hand further stabilizes the interaction between TFIID and promoter DNA Through the interaction with the zinc ribbon ... neutralizes some of the positive charges of the histone tails and therefore reduces the affinity of the histone tails to DNA The histone tails are then displaced from the nucleosomes causing the nucleosomes ... TFIIA and TFIIB (Tjian and Maniatis, 1994) These physical interactions are important for the recruitments of the basal transcription factors TFIID, TFIIA and TFIIB to the PIC and stabilize the...
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X ray crystallographic study of yeast dcp1 and dcp2 proteins insights into the mechanism and regulation of eukaryotic mRNA decapping
Ngày tải lên :
14/09/2015, 12:28
... both yeast and human Dcp2 proteins, the efficiency of decapping is in correlation with the length of the substrate It is hypothesized that the special separation of the RNA binding site and the ... residues forms the PRS binding site The PRS ligands adopt a left-handed PPII helix and the pyrrolidine rings of the proline residues on one edge of the PPII prism make contact with the conserved ... (Figure 7b) And the structure of the X29- m7GpppA complex shows that the adenine base of the cap analog is stacked in the active site and the triphosphate group is positioned close to the catalytic...
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Comparative genetic analysis of the transcriptional regulatory DNA of the oxytocin and vasopressin genes
Ngày tải lên :
15/09/2015, 22:13
... works and how the machinery itself evolves In this introduction, I describe in some detail the oxytocin and vasopressin peptides, and the structure and evolution of the genes encoding them Then ... includes the exons and introns, 5’ and 3’ UTR, and the promoter and regulatory DNA The ‘promoter’ is the stretch of DNA from approximately –40 to +10 bp of the transcription start site; it is the ... through the level of the line in a) The optic chiasma (the point where the optic nerves cross over, before ascending to the optic tecta) is represented in grey The third ventricle and the periventricular...
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The Design and Implementation of a Log-Structured File System
Ngày tải lên :
12/09/2012, 15:05
... writes, then reads the file back sequentially, then writes 100 Mbytes randomly to the existing file, then reads 100 Mbytes randomly from the file, and finally reads the file sequentially again The bandwidth ... for the log can be generated either by copying the old blocks or by threading the log around the old blocks The left side of the figure shows the threaded log approach where the log skips over the ... are overwritten If the count falls to zero then the segment can be reused without cleaning The blocks of the segment usage table are written to the log, and the addresses of the blocks are stored...
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The Breadth and Depth of DSP
Ngày tải lên :
13/09/2012, 09:49
... controlling and mixing the signals in these many elements, the sonar system can steer the emitted pulse to the desired location and determine the direction that echoes are received from To handle these ... transferring their customer's information, while they must pay to establish and maintain the channel The financial bottom line is simple: the more information they can pass through a single channel, the ... generation and recognition are used to communicate between humans and machines Rather than using your hands and eyes, you use your mouth and ears This is very convenient when your hands and eyes...
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