transcription translation stress responses and protein degradation

... miRNAs 399 and 2111 Phloem responses are compared to data obtained from leaves and roots All data were obtained from miRNA array hybridization experiments Differences between stress and control ... mature miR395, miR399 and miR171 by RNA gel blot analysis in scions and rootstocks of reciprocal hen1-1/WT and WT/hen1-1 grafts under sulfate and phosphate deficiency A: miRNAs 395 and 399 were translocated ... earlier than others, and that the translocated miRNAs serve to coordinate physiological responses with plant parts that are not yet stressed and therefore not yet synthesize stress- responsive miRNAs...

Ngày tải lên: 12/08/2014, 03:21

... reactioncenter protein PsaA was stable until h and then decreased very slightly with further illumination However, PsaB protein degradation started after a 1-h exposure and a degradation product ... photoinhibition of PSI coincided with the damage of both reaction-center proteins, PsaA and PsaB, and the proteins PsaD and PsaE on the acceptor side of the photosystem [22] In plants, reactive ... histidine, this protein declined by 45 and 60% after and h of illumination, respectively, while with n-propyl gallate, the degradation of this submit was only about 40% after and h illumination...

Ngày tải lên: 16/03/2014, 18:20

... roots and shoots of pea Five-day-old pea plants were given heat stress (42 °C), low temperature stress (4 °C), salt stress (0.3 M NaCl) and osmotic stress (0.3 M mannitol) for 24 h and roots and ... required amount of protein at room temperature for 10 DNA protein complexes formed were fractionated by 5% nondenaturing PAGE and autoradiographed To abolish any protein protein interaction, ... protein A sepharose beads and the resulting supernatant was used for phosphorylation assay RT-PCR Approximately 0.5 g plant tissue (control and stress treated) was ground in liquid nitrogen and...

Ngày tải lên: 18/03/2014, 01:20

... shows SR protein the way out Molecular Cell 20, 499–501 46 Anderson P & Kedersha N (2002) Visibly stressed: the role of eIF2, TIA-1, and stress granules in protein translation Cell Stress Chaperones ... Tai-Huang Huang and Konan Peck for the SARS-CoV N protein cDNAs, plasmids and recombinant proteins, and Chwan-Deng Hsiao and Yi-Wei Chang for CD analysis We thank Ru-Inn Lin and Wei-Lun Chang ... modulates the activity, protein protein interactions and subcellular localization of SR proteins [29] Coronavirus N proteins are phosphorylated in host cells and in virions [25,30,31] and it has been...

Ngày tải lên: 23/03/2014, 07:20

... Lipoprotein and lipoprotein oxidation The very-low-density lipoprotein and low-density lipoprotein (LDL) fractions were isolated from serum by ultracentrifugation at a density of 1.063 g/ml and ... immunoreactivity (as seen in lanes and 6) Two bands of lowmolecular-weight DNP immunoreactive bands were observed in control and Cu-stimulated lipoproteins, and glucosamine had no discernable ... bands to MDA2 Increased reactivity and appearance of lowmolecular-weight aldehyde -protein adducts suggest activation-dependent aldehydic protein oxidation and protein fragmentation In comparison,...

Ngày tải lên: 09/08/2014, 10:20

... given time point during the stress response, and 5′, mid, and 3′ CDS PolII enrichments were calculated for activated and repressed genes before and after 30 minutes of stress (Figure 3; Additional ... b) PolII enrichment was calculated for 5’, mid-, and 3’ CDS at t = (pre -stress) and t = 30 minutes (stress) for heat shock (a), and diamide (b) stresses Genes are ordered by mRNA change from highly ... transcription is induced in stress (see, for example, altered SUT expression in stress in [36]) As genome-wide datasets that identified CUTs and SUTs in budding yeast were not derived under stress...

Ngày tải lên: 09/08/2014, 20:22

... BNST and CeA but also reduce corticotrophin-releasing hormone mRNA in the dorsal part of BNST, and thus the BNST and CeA are the part of extra-hypothalamus-pituitary gland-adrenal gland stress ... Harvey BH, Brand L, Jeeva Z, Stein DJ: Cortical/hippocampal monoamines, HPA-axis changes and aversive behavior following stress and restress in an animal model of post-traumatic stress disorder ... μ- and δ-opioid receptors were shown to mediate thermal analgesic, tailwithdrawal, and heat-induced tail-flick responses [18] Thus, both μ- and δ-opioid receptors may have a crucial role in stress...

Ngày tải lên: 10/08/2014, 05:21

... Inflammation 2009, 6:20 Background Protein Never in Mitosis Gene A Interacting-1 (PIN1) is an enzyme that regulates transcription, and turnover of mRNA and proteins PIN1 is a cis-trans peptidyl-prolyl ... phospho-proteins The conformational switching catalyzed by PIN1 allows it to regulate transcription factors, mRNA stabilization factors, and the susceptibility of a growing list of proteins to post-translational ... anti-PIN1 antibody and protein G agarose For pulldown, glutathione S-transferase or glutathione S-transferase-PIN1 fusion protein was added to 500 μg of cell lysate protein and glutathione-sepharose...

Ngày tải lên: 11/08/2014, 08:22

... ABRE), and recognition sites for MYB, MYC and WRKY transcription factors (Additional file 7) These cis-elements (ABRE, MYBRS and MYCRS) and the corresponding transcription factors (AREB/ARF, MYB and ... salt, drought stress, and ABA treatment, conferring tolerance to drought and high-salinity stress in transgenic plants [27] In contrast, GmDREB3 is induced by cold stress only, and transgenic ... these transcription factors belong to the AP2/EREBP and MYB transcription factor family [13] Based on microarray analysis, genes involved in abiotic stress responses have been identified and categorized...

Ngày tải lên: 11/08/2014, 11:21

... (Electrophoretic Mobility Shift Assay) Both proteins were synthesized by an in vitro transcription and translation assay and biotinylated protein bands were detected by a chemiluminescent assay ... expression Although the protein- protein interaction between MYB and bHLH proteins has been already investigated in vitro [24-26], the mechanisms underlying the formation of the whole MBW transcriptional ... investigated in yeast and in grape suspension cells and compared to those of the native protein VvMYB5bR and VvMYB5bL capabilities to physically interact with the bHLH protein VvMYC1 were assessed...

Ngày tải lên: 11/08/2014, 11:21

... regulate multiple development processes and stress responses [11] Although the mechanisms of activation mediated by DREB proteins involved in plant stress responses are relatively well studied, ... cold and drought stresses This repression by RAP2.1 maintains tight control over these responses In Arabidopsis, RAP2.1 is transcriptionally activated by drought and cold stresses and binds to the ... drought and cold stresses RAP2.1 negatively regulates drought and cold stresses in Arabidopsis To investigate the in vivo role of RAP2.1 in modulating plant responses to drought and cold stresses, “loss...

Ngày tải lên: 12/08/2014, 03:21

... and abiotic stresses and the protein has been identified and isolated from the A thaliana apoplast together PR-1, PR-2 and PR-5 proteins [16] AtPNP-A has other features characteristic of PR proteins ... reactive oxygen species and changes in ion fluxes have been identified as early responses to both abiotic and biotic stresses, including an influx of H+ and Ca2+ and an efflux of K+ and Cl- [35] AtPNP-A ... response to various SAR eliciting and biotic and abiotic stresses The correlation of WRKY 46 in response to ion and osmotic abiotc stresses was both treatment and tissue specific In the light...

Ngày tải lên: 12/08/2014, 05:20

... lane 3, transcription with 50 ng of Tat; lanes and 5, transcription in the absence of Tat and with 10 nM or µM of okadaic acid; and lanes and 7, transcription in the presence of 50 ng of Tat and ... added; lane 3, transcription with 50 ng of Tat; lane 4, transcription in the absence of Tat and with 100 ng NIPP1; lane 5, transcription in the presence of 50 ng of Tat and 100 ng NIPP1 Transcription ... Flag-tagged CDK9 (lanes and 3), CDK9 T186A mutant (lanes and 5) and CDK9 C8A mutant (lanes and 7) Cells were labeled with (32P) orthophosphate in the absence (lanes 2, and 6) and in the presence...

Ngày tải lên: 13/08/2014, 09:21

... the paralogous proteins Scylla and Charybdis These two function in the insulin pathway downstream of PI 3-kinase and the protein kinase (and certified proto-oncogene) Akt/PKB and upstream of ... suppressors TSC1 and TSC2, resulting in the inhibition of the kinase Tor (target of rapamycin) and of downstream effectors such as ribosomal protein S6 kinase, thus suppressing cell growth Transcription ... cell growth Transcription of the Scylla and Charybdis genes is induced by hypoxia via the hypoxia-inducible transcription factor HIF The same induction by HIF and downstream effects on TSC1/TSC2...

Ngày tải lên: 14/08/2014, 14:21

... analyses included FN tests on the 20 wheat samples, and protein and ash determinations on the flours MATERIALS AND METHODS Grain and Flour Samples and Subsequent Treatments Experiment Determination ... Falling Number, Protein, Ash, and Moisture Tests Standard methods were used for the analysis of samples for FN, protein (N × 5.7), and ash by Approved Methods 56-81B, 46-30, and 08-01, respectively ... Z., and Pomeranz, Y 1994 Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles Cereal Chem 71:315-320 Batey, I L., Curtin, B M., and...

Ngày tải lên: 16/12/2012, 15:25

... message,calling inland and international,incremental services with the first number 8x Reward account allows customers to calling and sending messages within MobiFone network So that , all calling and sending ... (Argiculture and Rural Development Ministry) said that bridflu epidemic recurred in Cam Khe District (Phu Tho Provice) and Duy Tien District (Ha Nam Provice) which killed 124 chickens and purching ... had 150 chickens and 300 ducks died before that On Febuary 18th and 23rd, authority agencies also destroyed 1,115 ducks of Mr Nguyen Van Bay (Hiep Hung village, Binh Hai commue) and Mr Vo Van Đien...

Ngày tải lên: 09/04/2013, 10:29

... message,calling inland and international,incremental services with the first number 8x Reward account allows customers to calling and sending messages within MobiFone network So that , all calling and sending ... (Argiculture and Rural Development Ministry) said that bridflu epidemic recurred in Cam Khe District (Phu Tho Provice) and Duy Tien District (Ha Nam Provice) which killed 124 chickens and purching ... had 150 chickens and 300 ducks died before that On Febuary 18th and 23rd, authority agencies also destroyed 1,115 ducks of Mr Nguyen Van Bay (Hiep Hung village, Binh Hai commue) and Mr Vo Van Đien...

Ngày tải lên: 21/12/2013, 14:47

... two DEAD box RNA helicases, STRS1 and STRS2, were shown to improve Arabidopsis responses to multiple abiotic stresses, such as salt, osmotic stress, heat stress, and ABA [23] These investigations ... regulation of abiotic stress responses, and in the ABA signaling and stress- induced ABA biosynthetic pathways, respectively, in Arabidopsis [42–44] Further studies revealed that CBL1 and CBL9 functioned ... important for development and stress responses in Arabidopsis Plant Cell 17, 256–267 23 Kant P, Kant S, Gordon M, Shaked R & Barak S (2007) STRESS RESPONSE SUPPRESSOR1 and STRESS RESPONSE SUPPRESSOR2,...

Ngày tải lên: 14/02/2014, 18:20

... African 26 27 THE GLOBAL ECONOMIC AND FINANCIAL CRISIS: REGIONAL IMPACTS, RESPONSES AND SOLUTIONS The Global Economic and Financial Crisis: Regional Impacts, Responses and Solutions The Economic Commission ... banks have been poorly designed and implemented, and interbank markets in the US and Europe 33 THE GLOBAL ECONOMIC AND FINANCIAL CRISIS: REGIONAL IMPACTS, RESPONSES AND SOLUTIONS remain dysfunctional ... chaotic, and financial markets remain largely frozen Government bailouts of banks have occurred in several European economies – Belgium, Germany, Iceland, Ireland, the Netherlands, and the UK – and...

Ngày tải lên: 17/02/2014, 21:20