transcription factors stat5a b and stat3 in prostate cancer growth and metastases

báo cáo khoa học: " Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca)" pot

Ngày tải lên : 12/08/2014, 05:20

... junctions Length (bp) Intron I (phase 1) Intron II (phase 2) Coding sequence Pg MYB Pg MYB Pg MYB Pg MYB Pg MYB Pg MYB Pg MYB Pg MYB Pg MYB Pg MYB 10 Pg MYB 11 Pg MYB 12 Pg MYB 13 Intron I Intron II ... Domains Amino acids percentage PgMYB1/PtMYB1 PgMYB2/PtMYB2 PgMYB3/PtMYB3 PgMYB4/PtMYB4 PgMYB7/PtMYB7 PgMYB8/PtMYB8 PgMYB5/PtMYB14 PgMYB10/PtMYB14 PgMYB13/PtMYB14 Full coding sequences Identity Similarity ... http://www.biomedcentral.com/1471-2229/7/17 better understanding of their roles in gymnosperm lignin biosynthesis and wood formation MYB proteins have two structural regions, an N-terminal DNA-binding domain (DBD or MYB domain) and...

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Báo cáo khoa học: Structure of FocB – a member of a family of transcription factors regulating ﬁmbrial adhesin expression in uropathogenic Escherichia coli pdf

Ngày tải lên : 15/03/2014, 23:20

... of the FocB protein binding to DNA in the absence or presence of the DNA-binding compounds distamycin (minor groove-binding) and methyl green (major groove-binding), respectively A 401 bp DNA fragment ... N-terminal DNA-binding domain and a C-terminal oligomerization domain The DNA-binding headpieces of the N-terminal domains contain conventional major groove-binding HTH motifs, but symmetric hinge ... Gel mobility shift assays were performed to determine the DNA-binding properties DNA fragments containing the FocB-binding site were obtained by PCR using oligonucleotides yielding the 401 bp foc...

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Báo cáo khoa học: Nucleocytoplasmic shuttling of STAT transcription factors Thomas Meyer and Uwe Vinkemeier doc

Ngày tải lên : 30/03/2014, 15:20

... of a single amino acid residue in the amino terminal domain could reverse the defective nuclear accumulation of a DNA binding mutant without rescuing the DNA binding phenotype [4] These observations ... [27] It is interesting to note that binding of STATs to importin a5 does not appear to pose an obstacle to promoter binding and transcription,< /b> as STATtarget DNA can disrupt the importin a5 complex ... that Nipah and Hendra V proteins alter the subcellular distribution of STAT1 in resting cells and prevent nuclear import of both STAT1 and STAT2 in interferon-stimulated cells Thus, inhibition of...

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báo cáo hóa học:" Aberrant expression and potency as a cancer immunotherapy target of alpha-methylacyl-coenzyme A racemase in prostate cancer" pptx

Ngày tải lên : 18/06/2014, 15:20

... were stained with streptavidin-biotin complex (Nichirei), followed by incubation with 3,3diaminobenzidine and counterstaining with hematoxylin The same tissues were immunostained with an antiprostate-specific ... suspended with mL of PBS containing 1% formaldehyde, and analyzed by FACScan (Becton Dickinson, Mountain View, CA) Binding affinity was evaluated by comparing mean fluorescence intensity (MFI) of ... AMACR3 (FYELLIKGL) and AMACR4 (IYQLNSDKII) carrying the HLA-A24 binding motif (Figure 3A) Next, we assessed their binding affinities to HLA-A24 molecules by a binding assay using TAP-deficient...

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Báo cáo khoa học: "The Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation" pot

Ngày tải lên : 09/08/2014, 09:20

... Equal protein loading was confirmed by Ponceau S staining Blots were blocked in TBS buffer containing 0.05% Tween 20 and 5% non-fat dried milk for h at room temperature The membrane was incubated ... JH, Beijnen JH, Sindermann H, Engel J, Dubbelman R, Moppi G, Hillebrand MJ, Bartelink H, Verheij M: Phase I and pharmacokinetic study of combined treatment with perifosine and radiation in patients ... cell numbers and longer incubation time with WST-1 resulted in an increased absolute absorption and smaller error bars A completely different picture was obtained when testing the antineoplastic...

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Báo cáo khoa học: "Early prediction of response to radiotherapy and androgen-deprivation therapy in prostate cancer by repeated functional MRI: a preclinical study" pptx

Ngày tải lên : 09/08/2014, 09:20

... by weights, representing the interneuron synapses in the brain Further, the BPNN architecture is divided into three layers; input, hidden and output layers The input layer feeds information into ... training the network, in order not to under- or overtrain it [25] Since our RMSE function (Figure 4A) from training, testing and validating the network showed curve flattening after a few training ... preclinical study in prostate cancer xenografts, and suggest that the combination of functional MRI parameters, in addition to standard clinical parameters, increases the power of predicting therapeutic...

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Báo cáo khoa học: "The evolution of rectal and urinary toxicity and immune response in prostate cancer patients treated with two three-dimensional conformal radiotherapy techniques" ppt

Ngày tải lên : 09/08/2014, 09:20

... http://www.ro-journal.com/content/6/1/87 Background Quality of life is becoming one of the most significant issues in treatment decision-making, in general, and more so in prostate cancer [1] Thus late rectal and urinary damage became ... (BD Biosciences) We included single-stain controls for further offline compensation Measurement and subsequent analysis was performed using FACSDiva (BD Biosciences) and TreeStar FlowJo (Ashland, ... Alber M: Incidence of late rectal bleeding in high-dose conformal radiotherapy of prostate cancer using equivalent uniform dose-based and dose-volume-based normal tissue complication probability...

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Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot

Ngày tải lên : 09/08/2014, 09:22

... of cells in G0/G1 is symbolised by a bar, G2/M by a white bar and S-phase by a black bar In the controls 15% of the cells were in S-phase, 65% in G0/G1 and 20% in G2/M In the following, the S-phase ... anti -prostate cancer activity in vitro and in vivo Clin Cancer Res 2004, 10:5282-5292 Ouchi H, Ishiguro H, Ikeda N, Hori M, Kubota Y, Uemura H: Genistein induces cell growth inhibition in prostate cancer ... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int...

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Báo cáo khoa học: "Acute and late toxicity in prostate cancer patients treated by dose escalated intensity modulated radiation therapy and organ tracking" pps

Ngày tải lên : 09/08/2014, 09:22

... the abdomen and pelvis and a total-body bone scan When clinically indicated a chest x-ray and/ or thoracic CT was performed Based on these examinations clinical staging was defined according to ... months) but always during RT Twenty-six patients received combined androgen blockade (CAB) consisting of antiandrogen and gonadotropin releasing hormone analogue (GnRH) Three patients received antiandrogen ... delivered to the rectum and bulb of the penis using MRI delineation for radiotherapy of the prostate Int J Radiat Oncol Biol Phys 2003, 57:1269-79 Sobin LH, Wittekind CH: The prostate In TNM Classification...

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Báo cáo khoa học: " Potentials of on-line repositioning based on implanted fiducial markers and electronic portal imaging in prostate cancer radiotherapy" docx

Ngày tải lên : 09/08/2014, 09:22

... (lines in the box) and the 10% (lower horizontal line), 25% (bottom of box), 75% (top of box) and 90% (upper horizontal line) percentile split in setup variability, prostate position variability and ... tion (bottom the box) errorand box) and horizontal horizontal line) in and totalLR, SIthe (black squares), (upper posi25% variabilityof box), split (top ofAP (lower 90%inthe median values (lines ... values10%variability, prostate line), format, Measured deviations in and Measured deviations in LR, SI and AP directions in a box plot format, showing the mean values (black squares), the median values (lines...

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báo cáo khoa học: " XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells" docx

Ngày tải lên : 10/08/2014, 10:20

... in prostate cancer cell lines We found that somatostatin and Octreotide up-regulated XAF1 mRNA and protein expression in prostate cancer cell lines The enhanced XAF1 expression by somatostatin ... PVDF membranes (Bio-Rad, Hercules, CA, USA) The membranes were first incubated with 5% nonfat milk in Tris-buffered saline (TBS) After washing three times in 0.1% Tween 20-TBS (TBST), the membranes ... somatostatin and Octreotide in prostate cancer cell lines To examine the regulatory effects of somatostatin and Octreotide on XAF1 mRNA and protein expression, prostate cancer cell lines (LNCaP,...

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Báo cáo y học: "Identification of candidate predictive and surrogate molecular markers for dasatinib in prostate cancer: rationale for patient selection and efficacy monitoring" doc

Ngày tải lên : 14/08/2014, 08:20

... therapeutics for prostate cancer candidate predictor of efficacy in both prostate and breast cancer Finally, the observed sensitivity to dasatinib of prostate cancer cell lines with expression of basal ... potency against leukemic cancer cell lines harboring BCR-ABL mutations [9], the clear and imminent need for overcoming imatinib resistance, and the profound clinical benefit demonstrated in clinical ... dasatinib sensitivity in cell lines, and being a target of the drug, EphA2 appears to be a strong candidate biomarker for dasatinib in prostate cancer Down-regulation of uPA expression by dasatinib...

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CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF TRACE AMOUNT OF SEX HORMONE RECEPTOR ACTIVE COMPOUNDS IN PROSTATE CANCER RELATED BIOLOGICAL SAMPLES

Ngày tải lên : 09/09/2015, 08:14

... 1g/L PBS/BSA Table 1 5b Intraday Validation of T in 1g/L PBS/BSA xiv Table 16a Interday Validation of DHT in 1g/L PBS/BSA Table 1 6b Intraday Validation of DHT in 1g/L PBS/BSA Table 17a Interday ... 3α-Diol in 1g/L PBS/BSA Table 1 7b Intraday Validation of 3α-Diol in 1g/L PBS/BSA Table 18a Interday Validation of 3β-Diol in 1g/L PBS/BSA Table 1 8b Intraday Validation of 3β-Diol in 1g/L PBS/BSA Table ... Table 4a Interday Validation of A4 in g/L of PBS/BSA Table 4b Intraday Validation of A4 in g/L of PBS/BSA Table 5a Interday Validation of T in g/L of PBS/BSA Table 5b Intraday Validation of T in...

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ADVANCES IN PROSTATE CANCER docx

Ngày tải lên : 22/03/2014, 22:20

... Activation in Prostate Cancer 589 Luis A Espinoza VII VIII Contents Section Cell Adhesion Proteins in Prostate Cancer 617 Chapter 25 Integrins in Prostate Cancer Invasion and Metastasis 619 Paulynn Chin ... Prostate Cancer Markers Chapter Testosterone Measurement and Prostate Cancer 157 Tine Hajdinjak Chapter Describing Prostate Cancer Dynamics: Second Look at PSADoubling Time and PSA-Specific Growth ... positive findings by Hoffman in men from Trinidad and Tobago can be probably explained by bias of select‐ ed controls [147] [148] iv Polyoma Viruses have been also associated with PCa: an ini‐ Is...

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báo cáo hóa học:" Can urinary exosomes act as treatment response markers in prostate cancer?" ppt

Ngày tải lên : 18/06/2014, 15:20

... produced by LNCaP -prostate cancer cell line Prostate cancer cell lines (LNCaP and DU145), as indicated, were maintained in culture as a source of positive-control prostate cancer exosomes (for subsequent ... advances are being made in understanding the biology underlying this disease, and in many respects in its treatment, there remains a need for better tools for PCa diagnosis and monitoring Disease-related ... findings of a pilot study, investigating urinary exosomes in prostate cancer patients We had two main aims in the study; firstly to assess the feasibility of using urine as an exosome source in...

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Báo cáo sinh học: " Downregulation of APOBEC3G by Xenotropic Murine Leukemia-Virus Related Virus (XMRV) in Prostate Cancer Cells docx

Ngày tải lên : 18/06/2014, 18:20

... protein XMRV is able to productively replicate in these cells Findings This report builds on and challenges the published data on the absence of A3G protein in prostate epithelial cells lines ... have originated by a rare recombination event during tumor passaging in mice Therefore, it has been proposed that XMRV is a laboratory contaminant and not a human pathogen Nevertheless, being a ... subjected to immunoblotting using anti-ApoC29 to assist in A3G identification in the gel for mass spectrometry The gel bands corresponding to A3G protein were excised and were subjected to in- gel...

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Báo cáo sinh học: " Prostate transglutaminase (TGase-4) antagonizes the anti-tumour action of MDA-7/IL-24 in prostate cancer" potx

Ngày tải lên : 18/06/2014, 19:20

... Nottingham, UK) and ABCAM (Cambridge, UK) ROCK inhibitor was from Santa-Cruz Biotechnologies, Inc (Santa Cruz, CA, USA), Akt inhibitor, SIS3 inhibitor, PLC-gamma inhibitor, JNK inhibitor, JAK inhibitor, ... vectorinduced expression of MDA-7 in human prostate cancer cells demonstrated varying degree of inhibition of growth and induction of apoptosis It is interesting to note that Bcl-2 and Bcl-xL ... HOE: Nuclear staining using Hoescht 33258 Comp.: Double immunofluorescent staining reaction obtained with each of the respective antibodies in B, C and D Magnification was ×100 Ablin et al Journal...

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báo cáo hóa học:" Prostate transglutaminase (TGase-4) antagonizes the anti-tumour action of MDA-7/IL-24 in prostate cancer" potx

Ngày tải lên : 20/06/2014, 03:20

... Nottingham, UK) and ABCAM (Cambridge, UK) ROCK inhibitor was from Santa-Cruz Biotechnologies, Inc (Santa Cruz, CA, USA), Akt inhibitor, SIS3 inhibitor, PLC-gamma inhibitor, JNK inhibitor, JAK inhibitor, ... vectorinduced expression of MDA-7 in human prostate cancer cells demonstrated varying degree of inhibition of growth and induction of apoptosis It is interesting to note that Bcl-2 and Bcl-xL ... HOE: Nuclear staining using Hoescht 33258 Comp.: Double immunofluorescent staining reaction obtained with each of the respective antibodies in B, C and D Magnification was ×100 Ablin et al Journal...

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