transcription factor and oncogene

... the zero, xN and 0.87xH frequencies, versus the sequence of the TTF-1 HD Measurements were done at 11.7 T and 286 K Blank slots are for residues 29 and 42 (prolines) and Glu17, Leu34 and Lys46, ... (9.84 ± 0.20 ns) and two for sgi (0.26 ± 0.03 ns and 0.55 ± 0.06 ns) (supplementary Doc S1 and Fig S2) Comparison of results from MF and RSDM The results for sm obtained by the MF and RSDM approaches ... surfactant protein B gene promoter is a target for thyroid transcription factor and hepatocyte nuclear factor 3, indicating common factors for organspecific gene expression along the foregut axis...

Ngày tải lên: 18/02/2014, 16:20

... family and CRE binding 6428 factors involved in the NFjB pathway and other functional related factors, such as p300 and CBP Group D contain factors related to cell cycle and DNA repair-related factors ... TAFII and NC2 and NF-Y factors that have histone fold motifs [52] Group G is a small subset that contains all the SMAD proteins of the HTFN and APC and b-catenin-related factors Groups C and D ... 2005 FEBS C Rodriguez-Caso et al Human transcription factor network topology Table Description and functionality of transcriptions factor hubs Transcription factor (TF), degree (k), betweenness...

Ngày tải lên: 19/02/2014, 07:20

... exponential growth phase, and the cells were then washed and resuspended in SMM without nitrate, and finally incubated for a further 20 Samples were taken before and after the shift, and soluble cell-free ... assimilatory enzymes glutamine synthetase (GS) and glutamate synthase, respectively [6–8] TnrA belongs to the MerR family of transcription factors, and is present as a homodimer of two 12-kDa subunits ... recognition and, at the same time, overlaps with the GlnK TnrA, a major transcription factor in B subtilis for the control of nitrogen assimilation, is active under nitrogen-limited conditions and is...

Ngày tải lên: 06/03/2014, 00:21

... pathway and HO-1 regulation in PC12 cells [29,30] This may be through nuclear factor E2-related factor- 2 (Nrf2), a member of the cap’n’collar family of basic leucine transcription factors and a ... different transcription factors that may be involved in mediating the effect of insulin on HO-1 expression, including heat shock transcription factor- 1 (HSF-1), hypoxia-inducible factor- 1 (HIF-1) and ... pathway and its downstream effectors [23] This pathway is a key signal transducer of many growth factors and cytokines and has been implicated in the regulation of cell growth, cell migration and...

Ngày tải lên: 07/03/2014, 12:20

... d-Galinduced aging is still unknown Mitochondrial transcription factor A (TFAM) plays important roles in mtDNA replication and transcription, and the structure ⁄ organization of mitochondrial ... controlled by nuclear-encoded transcription and replication factors that are translocated to the mitochondria Both TFAM and mitochondrial-specific Pol-c are required TFAM is a key factor involved in directly ... 12-h light ⁄ dark cycle, and were fed standard rodent chow and water All experimental procedures were performed under the supervision of our Institutional Animal Care and Use Committee DNA extraction...

Ngày tải lên: 14/03/2014, 23:20

... regulated by Cdx homeodomain proteins and GATA factors [14–16] Thyroid transcription factor- 1 (TTF-1) is an important factor during lung morphogenesis [17–20] and drives the expression of several ... GATA factors in the regulation of Muc5b expression In addition to TTF-1, GATA factors and, especially, GATA-6 are important factors in lung morphogenesis and are known to regulate TTF-1 and synergize ... 10) and cold T84 (lane 16); supershift analysis with anti-GATA-4 (lanes 5, 11 and 17), anti-GATA-5 (lanes 6, 12 and 18) and anti-GATA-6 (lane 7, 13 and 19) IgGs The DNA–protein complex (GATA) and...

Ngày tải lên: 15/03/2014, 00:20

... PHL and ASR PHL and ASR PHL and HXPR PXHXH Transcriptional repressor of neuronal genes in non-neuronal tissues [39,40] Transcriptional repressor and activator [36,41] Transcriptional repressor and ... [49] Transcription factor IIE, a subunit (TFIIE a) [50] casein kinase II-a (CKII-a), ETS-1 and the a subunit of transcription factor IIE (TFIIE-a) (Table 2) REST and YY1 have one HXPR motif and ... Ets-1 in transcriptional regulation of transferrin receptor and erythroid differentiation Oncogene 21, 7933–7944 Ohkuma Y (1997) Multiple functions of general transcription factors TFIIE and TFIIH...

Ngày tải lên: 16/03/2014, 18:20

... by Sp family transcription factors [19] On the other hand, competitive bandshift demonstrated that the three Sp bands were only slightly reduced by Kmer and Lmer (Fig 6A, lanes and 6), whereas ... vectors, and by cell treatment with decoy oligonucleotides targeting Sp transcription factors Results Transcriptional organization of the human AbH-J-J locus and identification of the transcription ... Sp transcription factors In addition, Sp1 and Sp3 transcription factor chromatin immunoprecipitation demonstrated in vivo binding of these proteins to P1 promoter Our results suggest that Sp transcription...

Ngày tải lên: 23/03/2014, 07:20

... Mediator and TFIIA, but independent of TAFs Furthermore, PC4 is able to bind to double- and single-stranded DNA and to interact with TFIIB, TBP, TFIIA, Mediator, TFIIH and the gene-specific transcriptional ... double- and single-stranded DNA-binding activity Because the single-stranded DNA-binding activity can be competed away by a single-stranded DNA oligonucleotide, but not by a double-stranded one (and ... at °C, and the supernatants were used as a source of transcription factors and RNAPII The extent of the depletion was assayed by western (immuno) blotting Specific transcription assays Transcription...

Ngày tải lên: 23/03/2014, 07:20

... Cereghini, S (1996) Liver-enriched transcription factors and hepatocyte differentiation FASEB J 10, 267–282 Pontoglio, M (2000) Hepatocyte nuclear factor 1, a transcription factor at the crossroads of ... observed by the overexpression of the transcription factors, Pax8 and lim1, and this effect was additive [44] Furthermore, the artificial expression of Pax8 and lim1 in the Xenopus embryo induced ... the transcription factors, Pax8 and lim1, in Xenopus embryos led to the development of an abnormally large pronephros, and to the formation of ectopic pronephric tissue [44] As both these transcription...

Ngày tải lên: 23/03/2014, 13:20

... target and p52 or p50 NF-kB factors The double stranded PDP –PDP chimera mimicking the HIV-1 NF-kB binding sites does not inhibit the interactions between NFIL2, GATA-1 and STAT-1 transcription factors ... suppresses the expression of vascular endothelial growth factor, transforming growth factor beta1, and tissue factor and also cell growth and invasion activities Cancer Res 60, 6531–6536 Kawauchi, ... process, were 53 ^ 8C for PDP – PDP and 55 ^ 8C for DNA duplex (DNA) The double stranded PDP –PDP chimera inhibits the interactions between NF-kB transcription factors and target DNA–DNA molecules When...

Ngày tải lên: 24/03/2014, 04:21

... in CpG islands containing and TATA-free promoters [10–12] Typically, GC-rich promoter regions lack TATA or DPE core elements, and are frequently found to be bound with transcription factor Sp1 ... tube, skeletal and body wall defects in mice lacking transcription factor AP-2 Nature 381, 238–241 Eckert D, Buhl S, Weber S, Jager R & Schorle H (2005) The AP-2 family of transcription factors Genome ... SP1 and AP-2a regulate KCTD10 R Liu et al A B Fig Genomic structure of human and mouse KCTD10 genes, and the 5¢-flanking region sequence of human KCTD10 (A) Genomic organization of human and mouse...

Ngày tải lên: 30/03/2014, 02:20

... Ets factors may direct the choice of the promoter elements in play, and therefore, determine the selective combination of transcription factors involved and the regulatory pathways modulating transcription ... inhibits transcription Curr Opin Genet Dev 15, 536–541 Pastorcic M & Das HK (2000) Regulation of transcription of the human presenilin-1 gene by ets transcription factors and the p53 protooncogene ... the transcription factors with which it interacts ERM recognizes specifically Ets motifs located at )10 as well as downstream at +90, +129 and +165 on the PS1 promoter and it activates PS1 transcription...

Ngày tải lên: 30/03/2014, 09:20

... through a better understanding of the alteration and expression of transcription factors that regulate cellular growth, differentiation and apoptosis Brn-3 transcription factors (Brn-3a, 3b, 3c) ... POU transcription factor and inhibits its ability to activate transcription Cancer Biol Ther 2002, 1:428-32 18 Irshad S, Pedley RB, Anderson J, Latchman DS, Budhram-Mahadeo V: The Brn-3b transcription ... acquisition, analysis and interpretation of data, drafting and revising the manuscript AL and CBR assisted with experiments, interpretation of data and manuscript preparation JFK and MAQ assisted...

Ngày tải lên: 20/06/2014, 07:20

... cultures in normal oxygen the gene expression of collagen types II, IX, X and XI and the transcription factors SOX6 and SOX9 was greatly increased (p < 0.05 or p < 0.001) in comparison with monolayer ... lowered oxygen tension, and this was correlated with the selective upregulation of HIF2α and increased expression of the key chondrogenic transcription factors SOX9, SOX5 and SOX6 Immunohistochemistry ... expression for hypoxia-inducible transcription factors (HIF) and cultures of infrapatellar fat pad cells SOX genes (a), collagens (b) and proteoglycans (c) in monolayer culture and after chondrogenic differentiation...

Ngày tải lên: 09/08/2014, 10:20

... Arabidopsis and Petunia, MYB transcription factors require a bHLH partner for the trans-activation of flavonoid structural genes [17,21] Recently, two bHLH transcription factors (VvMYC1 and VvMYCA1) and ... with chromatin remodelling factors, general transcription factors (GTFs) of the RNA polII pre-initiation complex, and can also affect initiation of the transcription and elongation [44,45] The ... such transcription factor is poorly understood, but the few available studies suggest that they may influence both DNA binding affinities and transactivation properties of transcription factors...

Ngày tải lên: 11/08/2014, 11:21

... subgroup (AtMYB30, 31, 94 and 96) On the other hand, the grape accession protein ABK59040 was closely related to AtMYB30 and AtMYB31 and to a lesser extent to AtMYB94 and AtMYB96 (Figure 1A) Hereafter, ... ACF21938 and ABK59040 as VvMYB60 and VvMYB30, respectively Based on these results, we further divided Subgroup1 into Subgroup 1.1, (AtMYB30, AtMYB31, AtMYB94 and AtMYB96) and Subgroup 1.2 (AtMYB60 and ... first intron and the third exon, which were longer in the grape genes compared to AtMYB60 Expression of VvMYB30 and VvMYB60 in grape tissues and in response to hormonal and stress factors qPCR...

Ngày tải lên: 11/08/2014, 11:21

... revealed four tandem R2R3 MYB genes as likely candidates for the authentic R gene R2R3 MYB transcription factors are one of the largest transcription factor families in plants [41,44], and specific ... basic-helix-loop-helix (bHLH) transcription factors and WD40 proteins It is unclear if the R gene product acts to promote transcriptional activation of both ANS and UF3GT genes, or if activation ... emerged is the regulation of critical structural genes by R2R3 MYB transcription factors in complexes with bHLH transcription factors and WD40 proteins [48] R2R3 MYB genes tend to display limited...

Ngày tải lên: 11/08/2014, 11:21

... binding / transcription factor ANAC019 (Arabidopsis NAC domain containing protein 19); transcription factor ANAC012/NST3/SND1 (ARABIDOPSIS NAC DOMAIN CONTAINING PROTEIN 12); transcription factor ... woody and herbaceous stems and emphasize the importance of expression thresholds in transcription factor studies Background Plant fibres constitute a valuable renewable resource for pulp, paper and ... of water, and xylary fibres, which provide mechanical strength and which make up the bulk of woody biomass [2] Wood density and chemical composition, fibre and vessel length, diameter and wall...

Ngày tải lên: 11/08/2014, 11:21

... WRKYGCM1 zinc fingers and the relationship between transcription factors and transposons Nucleic Acids Res 2006, 34(22):6505-6520 Ulker B, Somssich IE: WRKY transcription factors: from DNA binding ... bZIP transcription factors, TGA5, TGA6 for SA [85-87]; allene oxide cyclase (AOC) [88] and plant defensin 1.2 (PDF1.2) for JA [50]; ethylene insensitive (EIN2) [89] and ethylene responsive factor ... Arabidopsis WRKY25 and WRKY33 transcription factors in abiotic stresses Plant Mol Biol 2009, 69(1–2):91-105 Ramamoorthy R, Jiang SY, Kumar N, Venkatesh PN, Ramachandran S: A comprehensive transcriptional...

Ngày tải lên: 12/08/2014, 03:20