... the performance of TransCont we note that much of the loss is a result of the lack of variability in the translations which is a key factor in the performance of the algorithm For example for the ... SV3AW data Compared to state of the art systems, RelCont with an overall F-measure performance of 62.13% outperforms the best unsupervised system of 57.5% UNED-AW-U2 for SV2 (Navigli, 2009) It ... inconsistencies in the annotations of nouns in SemCor or the very fine granularity of the nouns in WN We know that 72% of the nouns, 74% of the verbs, 68.9% of the adjectives, and 81.9% of the adverbs directly...
... versus 5.7% for placebo) No difference in event rates for other types of adverse events Bernard et al 1997 [44]: Ibuprofen No adverse findings noted Second episodes of sepsis occurred more often in ... wide range of disease severity and risk for death, as long as patients enrolled have evidenceof sepsis-induced organ dysfunction(s), patients are at an appreciable risk for death (e.g as evidenced ... interpretation of the overall population and, more importantly, of reported subgroups Severe sepsis was defined in all studies as follows: the presence of known or suspected infection; evidenceof a systemic...
... fragment for use as a positive control of the XMRV genome (Genbank acc num EF185282) Conditions of first round PCR for the detection of proviral sequences were: 100 ng patient DNA, primer Out -For ... PCR for sensitive screening of patient tumor tissue DNA Nested PCR for sensitive screening of patient tumor tissue DNA (A) A nested PCR primer setup was used as indicated for the screening of ... patients for antibodies could therefore offer a rapid and valid screening method to investigate the involvement of a virus Obviously the determination of sensitivity and specificity of these...
... binding for M2+ (M ¼ Zn, Cd) initially to the a domain, thereby leading to formation of the M4a cluster prior to formation of the M3b cluster, demonstrating that the individual binding constants of ... values of 0.0, 0.8, 1.6, 2.4, 3.2 and 4.0 of Cd2+ at 22 °C Inset: Plot of changes in CD intensity monitored at 223, 240, 250 and 263 nm as a function of molar equivalents of Cd2+ up to a maximum of ... show the formation of intermediate Cd1a, Cd2a and Cd3a species, with the Cd4a species forming only after > equivalents of Cd2+ have been titrated Addition of 4.0 equivalents is required for 100%...
... Madin–Darby canine kidney (MDCK) cells, a study of chimeric forms of DPP IV has shown that the luminal domain of DPP IV carries dominant apical sorting information while the short cytoplasmic tail ... targeting is beginning to be perceived For instance, one striking example of c-Src location inside one organelle has been reported for the inner membrane of mitochondria of osteoclasts [57] Moreover, ... concentration of the soluble form of DPP IV may result in an increased proteolysis of GLP-1 peptides and thus decreased insulin secretion [20] The insulin-dependent phosphorylation of DPP IV observed...
... spans of TatA and TatB Deletion of the TM spans of TatA or TatB leads to a loss of activity [31] but the important characteristics of these regions have not been probed We constructed a series of ... analyzed for export of TorA-GFP using the assay protocols detailed in Fig For clarity, mutations in TatA are shown underlined and in grey (B) Combinations of mutations in the amphipathic regions of ... proton motive force [23] On the basis of these studies, it has been proposed that binding of substrate to the TatBC subunits triggers the recruitment of the separate TatA complex to form an active...
... Amount of products formed (mol percentage) are shown conducted in the absence of Tween 80 Finally, in the presence of Tween 80, oxidation of a-keto diarylpropane (X) resulted in the formation of ... over 90% of the added diarylpropane I was oxidized in the presence of Tween 80, while in the absence of Tween 80, 24% of the diarylpropane I was oxidized In addition, while about 30% of the diarylpropane ... Oxidation of diarylpropane substrates by MnP Time courses for the oxidation of diarylpropanes I and VI by MnP, in the presence and absence of Tween 80, are shown in Fig After 12 h of incubation,...
... methylation of the C-terminal half of the a1(I) resulted in either reduced binding for a1(I) CB7, or a complete loss of the binding for a1(I) CB6 The variation of the binding ability for N-methylated ... were analyzed for purity by means of a quantitative Hyp assay [38], electrophoresis in denaturing conditions [34], N-terminal sequencing for some peptides and for conformation by means of CD spectroscopy ... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of...
... were subjected to one cycle at 95 °C for min; 40 cycles at 95 °C for 30 s, 60 °C for 60 s and 72 °C for 90 s; and a ®nal additional extension period at 72 °C for Ampli®ed DNA fragments were puri®ed ... activity of this mutant enzyme could not be restored by the addition of Tween 40 [39], it can be concluded that the 482 Gly residue is important for the activity of the enzyme, likely being one of ... different carB alleles This provides genetic evidences for the multimeric organization of the enzyme phytoene dehydrogenase in Phycomyces The nature of the mutations in the complementing carB alleles...
... the major circulating form of vitamin D, 25(OH)D, into the active hormonal form, 1,25(OH)2D So, there is a local mechanism in many tissues of the body for converting the form of vitamin D in the ... treated for an early stage of breast cancer have been randomized into two groups The dietary goals for the test group of the study are servings of vegetables, 16 oz of vegetable juice, servings of ... effect of fruits and vegetables was found in 128 of 156 studies that gave relative risks For most cancers, people in the lower quartile (1/4 of the population) who ate the least amount of fruits...
... spectrum of the turnover form of the T2Cu center are slightly different from that of the as-puried form (cf Table 2) which suggests the existence of modications in the catalytic center of the enzyme ... recent model for the catalytic mechanism of Cu-Nir supposes that nitrite binds to the oxidized form of the T2Cu center, displacing a solvent molecule and forming a hydrogen bond between one of its ... allowed, for the rst time, the determination of the redox potentials of both copper centers Our studies suggest that the presence of substrate plays an important role in the modulation of the redox...
... providing kinetic evidencefor the direct complex formation of P450nor with NADH Materials and methods Mutagenesis and expression plasmids The construction of each mutant of P450nor of F oxysporum ... pocket of P450nor (Eur J Biochem 271) 2891 Fig Formation of the spectral intermediate (I ) observed upon + reduction of the Fe3 –NO complex of the D393A mutant with a higher concentration of NADH ... follows its formation, although the rate of decomposition is much slower than that of I formation (cf Figure 6) hydrogen bond network containing Asp393 is essential for the binding of NADH rather...
... 0.004 0.003 0.002 0.001 Cg-BMPR1 Cg-TGFβsfR2 0.04 number of copies of Cg-TGFβsfR2 per copy of GAPDH 0.25 number of copies of Cg-BMPR1 per copy of GAPDH 0.005 st ul a G as Tr tr ul oc a op ho re L ... 0.006 yt es 1.0 0.007 B la 1.2 O oc 1.4 number of copies of Cg-TGFβsfR2 per copy of GAPDH Cg-TGFβsfR2 O oc number of copies of Cg-BMPR1 per copy of GAPDH Cg-BMPR1 0.2 0.15 0.1 0.05 0.035 0.03 ... promiscuous receptor Evidencefor the conservation of the mechanism of mesoderm induction in molluscs and across bilaterians Although Lartillot and colleagues have observed for a number of developmental...
... by the carbonyl oxygen of G168 Inclusion of F221 obscures the local structure in the proximity of T171 and for clarity is omitted Table Comparison of functional parameters for HRPC proximal pocket ... wild type [16,17] The pKa for the alkaline transition being similar to that of the wild type, % 11.1 [18] Finally, comparison of the X-ray structures of the oxidized forms of the native [5,19] and ... into the role of key catalytic residues [69] A signicant outcome of many of these studies [6,1012] has been the discovery of coupled effects that can be understood on the basis of a hydrogen...
... 14693–14698 Van Hoof, C., Ingels, F., Cayla, X., Stevens, I., Merlevede, W & Goris, J (1995) Molecular cloning and developmental regulation of expression of two isoforms of the catalytic subunit of protein ... templates for DNA sequencing 5¢-RACE technique 5¢-RACE reactions were performed on about 1.5 lg of total RNA of prophase X laevis oocytes with U of AMVreverse transcriptase (5¢/3¢RACE kit, Roche) For ... reaction was performed using primers containing the translation stop of the different isoforms to obtain specific cDNA After purification of this cDNA, semiquantitative PCR was performed PCR products...
... moreover attach most often to the lowest, nearest N P is important for the semantic interpretation of sentences because of the combinatorial explosion of possible attachments of prepositional phrases ... resemble more spoken informal discourse than written formal discourse The difference in number of occurrences per I000 words between the Wizard -of- OZ condition and the Informal Spoken condition ... $~ $$ 111441114 Occurrences per 1000 Words of Various Syntactic Features Wi~u~b .of- Oz Users avoided casual forms of language since they produced only % of fragmentary utterances, as opposed to...
... Association of Schools of Nursing Council of Deans and Heads of UK University Faculties for Nursing and Health Professionals Council of Deans of Nursing and Midwifery (Australia and New Zealand) Forum of ... and reform of the health workforce; and the recruitment and retention of international health professionals HWA has commenced work to develop a national training plan (NTP) for a number of health ... raise awareness of the key role of nurse education in the improvement of global health and quality of care See http://www.ganes.info GANES Members American Association of Colleges of Nursing Canadian...
... preferences of the lectins for agalactosylated N-glycans The specificity to agalactosylated N-glycans should help our understanding of the previously unknown mechanism of the functions of the DC-SIGN-related ... absence of DC-SIGN–Fc As shown in Fig 9B, the three major bands (i.e 1, of 160 kDa; 2, of 75 kDa; and 4, of 55 kDa) were present on a silver-stained gel, as well as an extra band (band 3, of 65 ... of 4b-phorbol 12-myristate 13-acetate (Sigma) After incubation for 96 h, cells were used for uptake and adhesion experiments To examine the surface expression of DC-SIGN on dTHP-1 cells and of...
... of calcium is important for the optimal activity of BsArb43B Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were performed to determine the Tm of ... different colours: blue for BsArb43B, magenta for BsArb43A, green for ABNTS, dark grey for AbnB, red for exo-Abn towards the solvent region, and presumably the other residues of the arabinohexose ... characteristic of this type of enzymes Superposition of the Ca trace of the BsArb43B FEBS Journal 277 (2010) 4562–4574 ª 2010 The Authors Journal compilation ª 2010 FEBS 4563 Role of calcium in...
... boiled at 95 °C for The sample for electrophoresis analysis was obtained by collecting the supernatant ( 30 lL) after centrifugation at 12 000 g for 20 s and addition of 10 lL of 75% (w/v) glycerol ... suggested, they represent ferritin and a product of degradation of the same protein [14] The same crossreactivity was also detected for a protein of twofold purified pea stem mitochondria (result not ... presequences of the chloroplastic protein transit peptide [28], similarly to what reported for pea ferritin [29,30] On the other hand, the analysis of the presequence of AtFer4 reveals a high score for...