towards modeling and therapy of genetic diseases using pluripotent stem cells

... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent ... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF...

Ngày tải lên: 09/09/2015, 18:56

... embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, induced pluripotent stem ... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... from healthy and disease-affected donor induced pluripotent stem cells (iPSCs) and epigenomic profiling can be used to evaluate how these genetic variants affect chromatin signatures, and transcription...

Ngày tải lên: 11/08/2014, 12:21

... and genetic approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells ... transposition system, in which a transiently expressed transposase catalyzes the excision of transgenes flanked by inverted terminal repeats [8,9] The PB system is effective in mouse and human cells, and ... Article 220 Abujarour and Ding 220.2 reduce the required number of excisions The efficiency and kinetics of iPSC generation using this method were similar to those observed using other viral vector...

Ngày tải lên: 14/08/2014, 21:20

... region, and used lentiviruses to induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers of ... of cells transitioning from fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent...

Ngày tải lên: 30/09/2015, 10:13

... experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the ... potential of these pluripotent stem cells for studying the fundamentals of early human development and organogenesis, for cellbased therapy and for disease modeling in the dish are a key focus of current ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... progenitor and stem cells for the treatment of neurological diseases and injuries, where the degeneration is widespread, like AD and HD Adult neurogenesis in neurological diseases and disorders ... in a broad range of neurological diseases and disorders (fig 1) The contribution and significance of this modulation to the etiology and pathogenesis of neurological diseases and disorders remain ... progenitor and stem cells for therapy [77] Conclusion and Perspectives Neuroinflammation is involved in the pathogenesis of neurological diseases and disorders, but its contribution and involvement...

Ngày tải lên: 08/08/2014, 17:20

... the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki T, Saido TC, Ito Y, Yokofujita J, Okeda ... after replating and could be cultured without the presence of glia or other cell types for an extended period of time iPSC-derived models of neurodegenerative diseases Page of 11 cells harboring ... m l of α-synuclein protein in the form of Lewy bodies and Lewy neurites, and selective loss of dopamin­ rgic (DA) e neurons in the substantia nigra of the mid­ rain [68,69] b The majority of PD...

Ngày tải lên: 11/08/2014, 12:21

... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... and AR50249 from to National Institute of Arthritis and Musculoskeletal and Skin Diseases to SCG and CHE, by grant STE 1051/2-1 from the Deutsche Forschungsgemeinschaft (DFG) to AFS and UN, and ... chondrocytes and in osteoarthritic cartilage [17], we were next interested in the appearance of live and apoptotic cells within our aggregate system after 10 and 21 days, which was visualized using...

Ngày tải lên: 09/08/2014, 14:22

... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... Jaenisch R: Genetic engineering of human pluripotent cells using TALE nucleases Nat Biotechnol 2011, 29:731-734 88 Zhao T, Zhang ZN, Rong Z, Xu Y: Immunogenicity of induced pluripotent stem cells Nature ... assessment of the epigenetic and transcriptional status of pluripotent cells This may be especially useful for the rapid monitoring of cell-line quality during large-scale production of iPSCs [71]...

Ngày tải lên: 11/08/2014, 12:21

... Materials and methods Isolation and expansion of mesenchymal stem cells DBA/1 mice were bred in the Animal Centre of the University of Leuven Bone marrow from 4- to 6-week-old DBA/1 and DBA/1 ... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor knockout ... mean (standard error of the mean) Differences were analyzed by the Mann-Whitney U test A P value of not more than 0.05 was considered significant Results Generation of mesenchymal stem cells and...

Ngày tải lên: 12/08/2014, 11:23

... HBO-103 mercury lamp and filter sets for FITC, Cy3.5, Texas Red, Cy5, Aqua, and DAPI Images were captured, processed, and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems GmbH, Altlussheim, ... (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and C colonies were 95.5%, 2.5% and 2.0% respectively for 48h exposure to 4oC (n=200); and ... the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC of the H9 line isolated by Thomson and colleagues...

Ngày tải lên: 31/10/2012, 16:57

... the developmental progression of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... core However, using an upstream primer that hybridizes just downstream of The commitment of pluripotent stem cells to successively less plastic progenitors and, finally, differentiated cells exhibiting...

Ngày tải lên: 07/03/2014, 12:20

... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... hES cells and adult cells was also significant (P < 0.05) In particular, the expression of miR-519b was 8-fold greater in hES cells than in EB cells and it was not even detected in adult cells...

Ngày tải lên: 18/06/2014, 15:20

... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... neurodegenerative diseases, autoimmune diseases and several forms of cancers [10] Phosphorylation and dephosphorylation of specific amino acid residues, including serine, tyrosine and threonine, ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 18/06/2014, 19:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... the density of Oil Red Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 2007, 2:15 O positive cells were calculated using Image Pro Plus software (Media ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated...

Ngày tải lên: 20/06/2014, 00:20

... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... neurodegenerative diseases, autoimmune diseases and several forms of cancers [10] Phosphorylation and dephosphorylation of specific amino acid residues, including serine, tyrosine and threonine, ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 20/06/2014, 04:20

... the stem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and ... mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy J Cell Mol Med 2004, 8:301-316 31 Tuan RS, Boland G, Tuli R: Adult mesenchymal stem cells and cell-based ... is critical for self-renewal of human multipotent adipose-derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin...

Ngày tải lên: 09/08/2014, 10:20

... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J Cell Biochem ... Cambridge, UK) and images acquired and stored with a Genesnap software (Syngene, Cambridge, UK) Statistical analysis The data are expressed as mean ± standard deviation and were analyzed using the...

Ngày tải lên: 09/08/2014, 10:23

... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... mechanism of pancreatic islets FN and LAM enhances activation of Akt and ERK The ECM bind to cells by activating signaling molecules such as Akt and ERK Therefore, we analyzed the effect of FN and ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM...

Ngày tải lên: 10/08/2014, 05:21