torsion i r injury in testis and oxidative stress

... Figure shows activities of AS, AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline ... confidence interval 149 Results The activity of NOS (Figure 1) was increased significantly in all the three brain regions indicating increased production of NO in anoxia In reperfusion group ... brain regions tested The activity of arginase (Figure 2) showed no significant change, indicating there was no increased utilization of arginine by this enzyme in anoxia However in reperfusion...

Ngày tải lên: 26/10/2012, 09:07

... transients during reversible brain ischemia Exp Brain Res 104, 462–466 188 Silver IA & Erecinska M (1992) Ion homeostasis in rat brain in vivo: intra- and extracellular [Ca2+] and [H+] in the hippocampus ... generation in the absence of respiratory chain inhibitors Numerous reports in isolated or in situ mitochondria support complex I being regarded as a major site of ROS production, however, a lingering ... Aconitase acts also as an iron-responsive element binding protein, isocitrate dehydrogenase is an RNA-binding protein, while kgd2p is a mitochondrial DNA binding protein [81–84] Mitochondria from...

Ngày tải lên: 07/03/2014, 12:20

... expression patterns of pea PsMPK2 in Arabidopsis revealed that its kinase activity increases in response to mechanical injury and other stress signals, including ABA, JA, and H2O2 [23] Recently, it was ... determined by 3,3¢-diaminobenzidine (DAB) and Nitro Blue tetrazolium (NBT) staining, respectively Under normal growth conditions, no obvious H2O2 was detected in either wild-type or transgenic ... staining (B) Abiotic stress- induced H2O2 and OÀ accumulation detected by DAB staining and NBT staining, respectively WT, wild-type role of GhMPK2 against oxidative stress was evaluated by testing...

Ngày tải lên: 14/03/2014, 23:20

... http://www.jneuroinflammation.com/content/1/1/21 Indomethacin Vitamin E Figure Representative pictures of brain sections from mice on placebo or receiving indomethacin plus vitamin E Representative pictures ... of indomethacin alone despite a significant reduction in brain inflammation had only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that lipid peroxidation ... Morgan D, Gordon MN: Microglia activation and βamyloid deposit reduction caused by a nitric oxide-releasing nonsteroidal anti-inflammatory drug in amyloid precursor protein plus presenilin-1 transgenic...

Ngày tải lên: 19/06/2014, 22:20

... recently cleared land covered before with natural vegetation, suggesting that this R similis-like population is present in the natural vegetation in Vietnam This last finding is very surprising, ... objectives are: 61 Advancing banana and plantain R & D in Asia and the Pacific, Vol 10 1) to find out if the R similis-like populations are indeed R similis, 2) if it is R similis, to see if they ... ed.) Singapore, CABI Publishing INIBAP 1997 Networking banana and plantain: INIBAP Annual Report 1996 INIBAP, France, 60 p 66 Advancing banana and plantain R & D in Asia and the Pacific, Vol...

Ngày tải lên: 09/07/2014, 03:20

... Carolina, Fraser r is being utilized in other major Christmas tree growing states such as Washington, Michigan and Oregon Christmas tree growers encounter different problems in their specific conditions ... for hybridizations, it was decided to further utilize this material At first, F2 material and new interspecific hybrids were obtained Part of this material is cultivated within the Breeding Station ... Antalya, IUFRO: 60 G L (1984): Program of interspecific r hybridization and its realization In: Proceedings of the National Seminar Hybridization and Variability of Forest Tree Species Zvolen,...

Ngày tải lên: 07/08/2014, 10:21

... support the production of amino- and organic acids directed to repair processes However, after 85 days, the NR activity in the seedlings was lower and decreased with increasing O3 At that time in ... a similar growth rate for seedlings in both light treatments Leaf morphology, structure and thickness are known to dier with increasing irradiance levels [2, 51] However, in our study, specic ... treatment) and in plantations (high irradiance treatment) We conclude that the assimilation rates of the seedlings were not limited by light availability, resulting in a similar total carbon gain and...

Ngày tải lên: 08/08/2014, 00:22

... Several factors contribute to VEGF production, including hypoxic conditions and stimulation by transforming growth factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes ... Endothelium injury is critical in SSc and is suspected to occur early in the disease process Circulating sVCAM-1 is a recognised marker of disease activity and a possible marker of disease severity [6,7,21]; ... sustained major decrease in oxidative stress in response to treatment with dihydropyridine-type calcium-channel blockers [17] The data reported herein confirm these results with a different design...

Ngày tải lên: 09/08/2014, 01:23

... chondrocytes: regulation by inducible nitric oxide Nitric Oxide 2008, 19:276-283 31 Windhaber RA, Wilkins RJ, Meredith D: Functional characterisation of glucose transport in bovine articular chondrocytes ... protein concentration Western blot analysis Whole cell lysates were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein ... normal and OA chondrocytes that had been incubated in high glucose medium for hour increased similarly when compared with their respective control cells maintained in RGM (Figure 7b) This indicates...

Ngày tải lên: 09/08/2014, 14:21

... burden is observed in chronic renal failure patients [1] The onset of this condition is during the pre-dialysis period, and it is particularly remarkable after dialysis therapy is initiated [1,2] ... alterations (7-C to 10-C) did not result in a significant difference in levels of oxidative stress biomarkers and the mtDNA copy number in dialysis patients Discussion An increased oxidative burden ... replication is initiated when CSBII forms a persistent RNA-DNA hybrid with CSBI and CSBIII regions [7] Nucleotide sequence analysis revealed that only a homopolymeric sequence in CSBII, ranging...

Ngày tải lên: 11/08/2014, 07:21

... number GAPDH Forward Reversed Forward Reversed Forward Reversed Forward Reversed Forward Reversed Forward Reversed Forward Reversed Forward Reversed Forward Reversed Forward Reversed Forward Reversed ... copper metabolism and oxidative stress in several gradations of hepatic copper toxicosis in Doberman pinschers Results To gain insight into the pathogenesis of copper toxicosis, we first measured ... new variant of primary copper toxicosis; (ii) there is a clear indication of a reduced copper excretion in the Doberman hepatitis group; (iii) there is a clear correlation between high copper levels...

Ngày tải lên: 13/08/2014, 13:20

... the tree are responsible for its anti-inflammatory properties [21,22] Suppression of leukotriene synthesis via inhibiting 5lipoxygenase is considered the main mechanism underlying their anti-inflammatory ... fibrosis as a complication, has been used in research to induce pulmonary fibrosis in mice and rats and produce an oxidative injury resulting in DNA damage and the destruction of alveolar epithelial ... specific and potent inhibitors of TGF-ß1 signaling in vivo [37] Moreover, preliminary investigations with this extract show considerable attenuation of BL-induced lung fibrosis when administered simultaneously...

Ngày tải lên: 13/08/2014, 14:20

... stabilization period (baseline) and every 10 minutes during the observation period In vitro measurements Determination by electron paramagnetic resonance (EPR) NO spin trapping Aorta and heart ... species [30-32] Since increased ROS formation is implicated in lipid peroxidation and oxidation of thiol groups, H2S, by decreasing ROS overproduction, may in fact limit tissue damage Our results show ... represented an easier route whilst avoiding side effects such as airway irritation In accordance with pilot experimentations in our laboratory and a previous study [19], a single intravenous bolus...

Ngày tải lên: 13/08/2014, 21:21

... DNase I (Invitrogen GmbH) and reverse-transcribed into cDNA using Supersript II Reverse Transcriptase (Invitrogen GmbH) according to manufacturer’s instructions TaqMan™ realtime polymerase chain reaction ... noted in the respective treatment groups at baseline UI, uninjured; SW, lung injury induced by saline washout; LPS, lung injury induced by intraperitoneal/intravenous E coli lipopolysaccharide Krebs ... observed with only very minor differences in IL-1b favouring HFOV This massive inflammatory response was also reflected by higher dose requirements of norepinephrine and additional fluid to maintain...

Ngày tải lên: 13/08/2014, 21:21

... function (e.g planning, monitoring, switching, activating, and inhibition) is associated with controlling emotion, cognition and action, disrupting education, work, home functioning and social relationships ... education, there were extremely high levels of misclassification for illiterate individuals Interestingly, only one study in this review distinguished cut-offs for literate and illiterate participants ... Asia INTRODUCTION Cognitive impairment ranges in severity, can occur at any point in a person’s lifetime, and can result in difficulties remembering, learning new concepts, concentrating, or...

Ngày tải lên: 22/12/2014, 16:52

... measuring mitochondrial respiratory function using a more representative substrate for glucose oxidation should provide additional insights into this interesting question Conclusions In summary, ... Metabolic gene switching in murine female heart function [8] Likewise, high fatty acid oxidation rates in the diabetic heart results in reduced cardiac efficiency [9] In addition to its cytoplasmic role, ... expression was normalized to 18S rRNA Mitochondrial isolation and respiration studies Animals were anesthetized using intraperitoneal sodium pentobarbital (50 mgÆkg)1) and heparinized to prevent...

Ngày tải lên: 18/02/2014, 16:20

... of surface-exposed residues Residues in italic indicate lack of conservation Location Anx(Gh1) Anx24(Ca32) IAB IDE IIAB IIAB IIDE IIDE IIIAB IIIAB IIIDE IVAB IVAB Trp32 Lys72 Arg103 Trp104 His144 ... basis of oxidative stress response by annexins Materials and methods Purification of recombinant protein Cloning and construction of an N-terminal His4-fusion protein has been described earlier ... structure of Anx(Gh1) (A) The fold of Anx(Gh1) as seen in a side view Domain I is coloured in dark blue, domain II in light blue, domain III in aquamarine, and domain IV in green Exposed surface residues...

Ngày tải lên: 17/03/2014, 03:20

... prevention of aggregation of insulin B chain following reduction of disulphide bonds [13], refolding of guanidine hydrochloride (or urea)-denatured proteins [12,14] and prevention of inactivation ... with lysine residues, whereas methylglyoxal reacts principally with arginine residues although modification of lysine and cysteine also occurs [2,28] H2O2 oxidizes methionine and cysteine residues ... supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose (50...

Ngày tải lên: 17/03/2014, 03:20

... to increase radical generation and thereby circumvent the difficulty in inducing oxidative stress by solely increasing pO2 [49] Menadione and paraquot are most frequently used to increase intracellular ... cysteines in two distinct ways: 1) by oxidizing adjacent sulfhydryl groups to form inter- or intra-molecular disulfide bods, and 2) by oxidizing cysteines to sulfenic acid and further oxidized derivatives ... Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited Virology...

Ngày tải lên: 12/08/2014, 04:20

... NPR1/NIM1-interacting prot (NIMIN1) Protein kinase family protein Pathogenesis-related protein (PR-5) Calreticulin precursor, Ca2+ binding SAR: Systemic acquired resistance; RBS: Responsive to biotic stress Page ... inhibitor/lipid transfer protein Wall-associated kinase (WAK1) Hin1 – role in hypersensitive response Mitochondrial protein of unknown function Zinc finger (C3HC4-type RING) family Calmodulin binding ... Aminotransferase, resp to wounding & JA Protein kinase family protein Phosphohistidine phosphatase activity Disease resistance, leucine rich-repeats Similar to YGL010w-like protein NPR1/NIM1-interacting...

Ngày tải lên: 12/08/2014, 05:20