... http://www.ora.com/catalog/pperl2 You should now have an idea of how easy LWP can be There are more examples at the end of this chapter, and the examples in Chapters See Example LWP Programs and all use LWP Right ... standard output: #!/bin /perl use LWP: :Simple; print (get $ARGV[0]); The first line, starting with #!, is the standard line that calls the Perl interpreter If you want to try this example on your own ... simple LWP examples, to give you an idea of what it makes possible Next, we'll list most of the useful routines within the LWP library At the end of the chapter, we'll present some examples...
Ngày tải lên: 24/10/2013, 08:15
... $response->error_as_HTML; } Since LWP: :RobotUA is a subclass of LWP: :UserAgent, LWP: :RobotUA contains all the methods as LWP: :UserAgent So we replaced the use LWP: :UserAgent line with use LWP: :RobotUA Instead ... LWP Let's try out some LWP examples and glue a few functions together to produce something useful First, let's revisit a program from the beginning of the chapter: #!/usr/local/bin /perl use LWP: :Simple; ... of example, and to show how easy it is, let's add support for the Robot Exclusion Standard to our previous example use LWP: :RobotUA; use HTTP::Request; use HTTP::Response; my $ua = new LWP: :RobotUA('hcat_RobotUA',...
Ngày tải lên: 24/10/2013, 08:15
Web Client Programming with Perl-Chapter 6: Example LWP Programs-P1
... a client may exhibit The examples in this chapter all use a simple command-line interface In Chapter 7, Graphical Examples with Perl/ Tk, we have some additional examples with a graphical interface ... describe to LWP what the action should be LWP handles it for you Many things, like handling URL redirection or handling HTTP version differences, will be handled automatically by LWP Also, the ... As you can see, LWP simplified a lot of the code Let's go over hgrepurl in a little more detail: #!/usr/local/bin /perl -w use strict; use HTTP::Status; use HTTP::Response; use LWP: :UserAgent;...
Ngày tải lên: 28/10/2013, 15:15
Web Client Programming with Perl-Chapter 6: Example LWP Programs-P2
... "$parent\n"; }fo } In the following chapter, we'll a few more examples, this time graphical examples using the Tk extension to Perl ... This help text Example: $0 -e me\@host.com -p http://proxy/ http://site_to_check/ USAGETEXT } package CheckSite; use HTTP::Status; use HTTP::Request; use HTTP::Response; use LWP: :RobotUA; use ... complete source of the CheckSite package, with some sample code around it to read in command-line arguments and print out the results: #!/usr/local/bin /perl -w use strict; use vars qw($opt_a $opt_v...
Ngày tải lên: 28/10/2013, 15:15
Perl & LWP pptx
... LWP history of: 1.2 History of LWP installation: 1.3 Installing LWP CPAN shell: 1.3.1 Installing LWP from the CPAN Shell manual: 1.3.2 Installing LWP Manually sample code: 1.5 LWP in Action LWP ... 9.1 Introduction to Trees libwww -perl project: 1.2 History of LWP license plate example: 5.5 POST Example: License Plates link-checking spider example: 12.3 Example: A Link-Checking Spider links ... The Basic Classes LWP: : module namespace: 1.2 History of LWP LWP::ConnCache class: 3.4.1 Connection Parameters LWP: :RobotUA: 12.2 A User Agent for Robots LWP: :Simple module: 2.3 LWP: :Simple document...
Ngày tải lên: 17/03/2014, 17:20
Perl & LWP pot
... LWP history of: 1.2 History of LWP installation: 1.3 Installing LWP CPAN shell: 1.3.1 Installing LWP from the CPAN Shell manual: 1.3.2 Installing LWP Manually sample code: 1.5 LWP in Action LWP ... 9.1 Introduction to Trees libwww -perl project: 1.2 History of LWP license plate example: 5.5 POST Example: License Plates link-checking spider example: 12.3 Example: A Link-Checking Spider links ... The Basic Classes LWP: : module namespace: 1.2 History of LWP LWP::ConnCache class: 3.4.1 Connection Parameters LWP: :RobotUA: 12.2 A User Agent for Robots LWP: :Simple module: 2.3 LWP: :Simple document...
Ngày tải lên: 23/03/2014, 00:20
o'reilly - perl & lwp library
... non-beta release of libwww -perl for Perl It was called release 5.00 because it was for Perl This made some room for Roy to maintain libwww -perl for Perl 4, called libwww -perl- 0.40 Martijn continued ... pages Example 1-1 shows how to fetch the O'Reilly home page and count the number of times Perl is mentioned Example 1-1 Count "Perl" in the O'Reilly catalog #!/usr/bin /perl -w use strict; use LWP: :Simple; ... HTML: :TokeParser Example 1-4 uses HTML: :TokeParser to extract the src parts of all img tags in the O'Reilly home page Example 1-4 Extract image locations #!/usr/bin /perl -w use strict; use LWP: :Simple;...
Ngày tải lên: 29/04/2014, 14:45
Safe carrying methods
... loads that are too heavy for you to lift or carry In these cases, you may need to use some of these methods: • Work with someone to carry the load • Use a trolley • Use a forklift or trolley jack ... as simple as being on the look out for things that may cause injury or harm to a person, for example, wet floors, lifting heavy loads and operating equipment and machinery Back Step - Determine ... lips, ears, neck and back of your hands need extra protection, so make sure these areas are properly covered with sunscreen Remember that you will need to reapply sunscreen after a few hours...
Ngày tải lên: 21/08/2012, 17:56
Perl và các khái niệm cơ bản
... goto Toán tử require Th viện Perl 5.0 Goodies Chỉ mục Trong chơng này: Lịch sử Perl Mục đích Perl Có sẵn Hỗ trợ Các khái niệm Dạo qua Perl Giới thiệu Lịch sử Perl Perl cách viết tắt cho Practical ... với Perl 4.0 (lần đa gần viết điều này) Mọi thứ làm việc với 5.0 sau Perl Trong thực tế, chơng trình Perl 1.0 làm việc tốt với gần đây, ngoại trừ vài thay đổi lạ cần cho tiến Mục đích Perl Perl ... Usenet, có lẽ có chỗ để dễ dàng công bố Perl cho giới Bên cạnh nhóm tin, bạn nên đọc tạp chí Perl, việc phân phối Perl Một nguồn có thẩm quyền khác sách Programming Perl Larry Wall Randal L Schwatrz...
Ngày tải lên: 31/08/2012, 09:39
Ngôn ngữ lập trình PERL
... [^\r\t\n\f] Trước bước vào nội dung Perl , áp dụng khám phá Chúng ta bắt đầu tạo ghi nhanh khác biệt nhỏ Unixperl Perl for windows NT hay Winperl Một khác biệt lớn hầu hết Perl script ta tìm đường dẫn ... biệt Perl , $_ , có điểm đặc trưng đònh riêng chúng thiết kế để làm cho Perl dể sử dụng 10 GUESTBOOK : Hãy bắt đầu với thí dụ đơn giản Guestbook dùng Perl Trước tiên bạn cần tạo file Perl gọi ... trình biên dòch Perl Perl chấp nhận kiểu số sau : - số nguyên - phân số - số âm - số mũ * Số nguyên : Không bắt đầu số Perl hiểu lầm sang số hệ 16 hệ số bắt đầu số * Chuỗi kí tự: Perl chấp nhận...
Ngày tải lên: 03/09/2012, 16:02
MOB Subject 5 –Access methods Static and dynamic access techniques
Ngày tải lên: 17/09/2012, 09:13
Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf
... Teflon tubing Equilibrate the column with buffer A or C Dialyze sample exhaustively or dissolve sample in buffer A or C and apply the sample to the column Run the column in a linear gradient up to ... Solution methods often crave larger amounts of material than the other two, but selective loss of components is less of a problem Membrane-based methods allow relatively large volumes of “dilute” sample ... Centrifugation in CsCl/4 M Guanidinium Chloride Dialyze samples against 10 vol of M guanidinium chloride extraction buffer The volume of the sample that can be run in each tube is two-thirds of the...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf
... the previously discussed examples, lectins are not necessarily as specific in their binding affinities as is suggested in commercial data sheets or the literature For example, peanut agglutinin ... Histological Methods for Detection of Mucin 39 Alternatively, prepared adhesive slides such as Superfrost Plus ®/(Menzel-Gläser, Braunschweig, Germany) may be purchased There are many other methods ... Histochemical Methods: Theory and Practice (45) provide comprehensive sections on useful histochemical stains We intend here to present only a limited selection of techniques by way of example Remember...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf
... duplicate or triplicate samples and standards (serial dilution of mucin in sample buffer) in assay buffer containing the detection antibody at the final dilution The sample/antibody mix can be ... preferable owing to more even sample distribution, greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated ... provided that the samples have not been overloaded (exceeded the membrane binding capacity or detection system capacity) Titration of samples may aid in quantitation Interassay control samples will...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf
... Original methods for measuring adherent mucous thickness in situ were based on (1) differences in refractive index between the mucous layer and the mucosal surface, measured by a slit lamp and ... respectively Consequently, the dimensions of mucous thickness measured by the slit lamp and pachymeter or pH gradient methods can be substantially greater than the actual values for the gel layer itself ... comparison of results by this method with those of previous methods used for staining the mucous gel can be found in Jordan et al (10) An example of the use of this method for studies in humans is...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf
... can be calculated per cell sample, z This can be done for each of the samples no and 2, and should yield similar values: z = d/a (sample no 1, au/mg) or z = h/e (sample no 2, au/mg) The level ... Tissue Sample No 2: Quantitation of Synthesis of Mature Mucin Cell or tissue sample no is pulse labeled with [35S]sulfate for 30–60 min, as described in Chapter 19 Homogenize the cell sample in ... can only be calculated from the duplo sample (i.e., [35S]amino acids–labeled, “type no 1” sample), and has thus to be corrected for different sizes of the samples (i.e., the protein concentrations,...
Ngày tải lên: 23/09/2012, 19:25
Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf
... acetonitrile 80 Thornton et al Methods 3.1 Extraction and Purification For a detailed description of extraction and purification, see Chapter In brief, mucins are extracted from samples at 4°C with M guanidinium ... mucin subunit samples enriched in specific MUC gene products and also to isolate different glycosylated forms (glycoforms) of a single MUC gene product (see, e.g., refs and 3) An example of the ... subunits phoretic mobility observed with the salivary mucin sample is not normally seen in respiratory mucin subunit preparations In respiratory samples there are typically two gel-forming mucins, namely...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf
... simplest form, is the overlaying of a small volume of sample onto preformed gradient of a supporting medium of increasing density The sample is centrifuged and a separation of different species ... acetic acid 13 Carbon rods 14 Vacuum-coating unit 2.3.2 Replicas Reagents as for Subheading 2.3.1 Methods 3.1 Extraction and Purification For a detailed description, see Chapter 3.2 Rate-Zonal Centrifugation ... mL/min/tube (see Note 6) Weigh tubes to check that they contain equal volumes Carefully apply sample (up to 500 µL) with a Hamilton syringe to the top of the gradient (see Note 7) Centrifuge...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf
... phase C: 90% (v/v) acetonitrile in water Methods 3.1 Sample Preparation and Hydrolysis Add an aliquot of a solution of a sample of mucin into an autosampler glass vial and dry under vacuum (see ... assignment of peaks (3) Sample preparation is an important step in amino acid analysis Ideally, samples should be dried and free of salts, amines, and detergents The sample of mucin must be desalted ... Allow the column to equilibrate with two gradient runs before the sample injection Inject an aliquot (≥5 µL) of each derivatized sample (see Note 6) Run time is 35 Allow at least for pump and column...
Ngày tải lên: 23/09/2012, 19:26
Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf
... Procise™ Methods 3.1 Sample Preparation Edman sequencing is a molar-dependent chemistry, and it is necessary to determine how many moles of glycopeptide are to be sequenced (see Note 3) Samples ... Elsewhere we have published alternative HPLC methods for the separation of PTH-glycosylated amino acids on a modified Beckman LF3600 sequenator (12) These methods were developed for two reasons: (1) ... methanol has evaporated, but before the membrane has dried, apply the sample in 10-µL aliquots (see Note 12) When all of the sample is applied dry the membrane 3.1.4 Covalent Attachment of Glycopeptides...
Ngày tải lên: 23/09/2012, 19:26