titration curve of oxalic acid and sodium hydroxide
Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
... Gymnemic acids inhibit rabbit glycer-aldehyde-3-phosphate dehydrogenase and induce asmearing of its electrophoretic band and dephosphoryla-tion. FEBS Lett 579, 4333–4336.Interaction of gymnemic acid ... average diameter of unbound GA is > 30 nm and that of GA and c-CD complex is 2.2 nm,similar to unbound c-CD, supporting the aggregate property of GA and the inclusion complexation of GA by c-CD.AbbreviationsCD, ... presence of 2.0 mM a-CD, b-CD, and c-CD. (B) The concentration dependence of c-CD on NMR spectra of 2.0 mM GA. The ratios of c-CD to GAare indicated.Y. Izutani et al. Interaction of gymnemic acid...
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Báo cáo y học: Esterified Hyaluronic Acid and Autologous Bone in the Surgical Correction of the Infra-Bone Defects"
... Dental Sciences and Surgery, University of Bari, Bari, Italy. Correspondence to: Prof. F.R. GRASSI, Professor and Dean, Department of Dental Sciences and Surgery - University of Bari, P.zza ... Hyaluronic Acid and Autologous Bone in the Surgical Correction of the Infra-Bone Defects Andrea BALLINI, Stefania CANTORE, Saverio CAPODIFERRO and Felice Roberto GRASSI Department of Dental ... grafting, 4 males and 5 females, all non smokers, with a mean age of 43,8 years for females, 40,0 years for males and 42 years for all the group, in good health and with a mean depth of the infra-bone...
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THÀNH PHẦN hợp CHẤT AXÍT béo và CAROTENOID của gấc fatty acid and carotenoid composition of gac (momordica cochinchinensisspreng) fruit
... findings of nonedetectable. The authors also reported 101 ( 38 and 22.1 ( 15.2µg β-carotene/g FW in extracted samples of aril and mesocarp,respectively, and 16 and 9 µg/g of zeaxanthin and 35 and ... identification of major carotenoids, includes carotenoidprofiles, measuring both trans- and cis-isomers of lycopene and β-carotene. We also provide a detailed fatty acid analysis of gac seed and aril, ... linoleic and R-linoleic acids.The aril also contains a significant, but varying, amount of stearic acid and small amounts of cis-vaccenic, myristic, eicosa-11-enoic, arachidic, and palmitoleic acids.Our...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... producingtransformant and was used for the purification of GALA.Growth of this transformant and subsequent purification of the enzyme were performed as described in Materials and methods. Purity of the preparation ... to facilitate purification of GALA. The enzyme has a molecular mass of 48.5 kDa and a pH optimum between 4 and 4.5. Incubations of ara-binogalactans of potato, onion and soy with GALA resultedinitially ... Maldi-TOF MS analysis of the hydrolysis of arabinogalactans by GALA. Three different arabinogalactans were used: potato (A), onion (B), and soy (C). Spectra were taken after 15 min (1) and 2 h (2) of...
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Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx
... protection of enzymes and membranes from thedetergent effects of excess fatty acids by sequesteringthem, and (d) modulation of cell growth and differenti-ation by transport of fatty acids to the nucleus ... uptake of fattyacids across the plasma membrane and transport tovarious subcellular organelles, (b) modulation of theactivity of enzymes involved in fatty acid metabolism,(c) protection of enzymes ... consists of four exons of 112, 174, 89 and 141 bp, coding for 22, 59, 30 and 20amino acids, respectively. The intron ⁄ exon structure of the fabp6 gene from zebrafish is consistent with that of all...
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Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx
... alternative route forthe production of UDP-glucuronic acid viamyoinositol has also been identified and characterized.Synthesis of UDP-glucuronic acid and UDP-xylose T. Oka and Y. Jigami2646 FEBS Journal ... amounts of the nucleotide sugars, and alsodiscuss the implications of our results in yeast for thestudy of metabolic regulation in plants.ResultsCloning and expression of AtUGD1 and AtUXS3genes ... inhibition constant (Ki )of AtUGD1. Inhibition by UDP-GlcA and UDP-Xyl wasmeasured with various concentrations of UDP-Glc and saturating levels of NAD+. Double-reciprocal plots of the data revealed...
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Báo cáo khóa học: Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A fluorescence and CD study docx
... ratio of native dimers to monomers, and the loss of H-bondinginvolving the strands bI of bLG [47], could be promoted inboth situations. A CD spectrum assigned to distortions of b-strands showed ... chirality of the environ-ments of the side-chains of aromatic residues (Trp, Tyr and Phe) and disulfide bonds [49]. The two deep peaks found at286 and 293 nm arise from Trp residues. In the case of ... means of a curve obtained using appro-priate fluorescence standards together with the standardprovided with the instrument. The quantum yields of NATA and bLG were determined relative to that of...
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Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt
... De-O-acylation of lipid AfractionsledtodecayofspeciesZandYandresultedinincrease of signals corresponding to ions of species X(data not shown). The total decrease of mass due to de-O-acylation of phosphorylated ... protons and protons from a, b and c positions of 3-hydroxy fatty acids are marked with;-CH ¼ CH-, -CH3 ,and- CH2-, a, b and c, respectively. CHCl3,DMSOandH2O represent signals from solvents and ... 2,3-diamino-2,3-dideoxyglucose(DAG) and a complex set of fatty acids (both ester and amide bound), as described previously [24], but alsogalacturonic acid and phosphate residues. The presence of GalA was unequivocally...
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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf
... Mendez, Cristina Paz and Ernesto J. Podesta´Department of Biochemistry, School of Medicine, University of Buenos AiresAlthough the role of arachidonic acid (AA) in the regulation of steroidogenesis ... of recombinantCTE-I as antigen dissolved in 0.5 mL of distilled water and mixed with equal volumes of Freund’s complete adjuvant,andthreetimeswith500lg of antigen mixed with equalvolumes of ... reports have shown that arachidonic acid (AA) and its metabolites play an essential role in the regulation of steroidogenesis and both the expression and function of StAR [17–26]. Adrenal cholesterol...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... independent regulation of these two genes and of the accumulation of bryonolic acid and phytosterols. It is noteworthy that the changes in theirmRNA levels correlated with the accumulation of therespective ... cycle. These results suggest independent regulation of these two genes and of the accumulation of bryonolic acid and phytosterols.Keywords: bryonolic acid biosynthesis; isomultiflorenolsynthase; Luffa ... growth, accumulation of bryonolic acid and total sterol, and mRNA levels of isomultiflorenol synthase and cycloartenolsynthase in the cultured Luffa cells. Although...
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Báo cáo khoa học: Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence doc
... to OPDA and JA synthesis [34]. Bycontrast, fractions of the unsaturated membrane fatty acid a-linolenic acid and a-linoleic acid are convertedrandomly and nonenzymatically to a variety of prod-ucts ... variety of abiotic and biotic cues.To deter feeding insects, nematodes and fungal and bacterial pathogens,plants have evolved a plethora of defence strategies. A central player inmany of these ... transition and the release of cytochrome c in animal cells [112]. In A549 humanlung adenocancer cells, Me-JA operates through theinduction of proapoptotic genes of the BCl-2, Bax and Bcl-X families and...
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Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc
... to 30% of the initial rate of UBHY-R,whereas the rates of synthesis of DNA and RNA were70% and 60% of the initial values, respectively. Thesedata suggest that the primary effect of eIF5A ... sites of p414GAL1 and the NdeI–BamHI sites of pET11a. Theentire ORF of the site-mutated and truncated eIF5A wassequenced for confirmation of the intended mutation ortruncation.Determination of ... mmNAD, 25 lg of BSA, 3 lCi of [3H]spermidine, 0.1 lgofDHS and 1.0 lg of DOHH and clarified BL21(DE3) lysatescontaining heIF5As (2–5 lg). After incubation for 2 h at37 °C, an aliquot of the reaction...
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Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
... onoxygenation of liganded human hemoglobinOxygen affinities of the a and b subunitsSergei V. Lepeshkevich and Boris M. DzhagarovInstitute of Molecular and Atomic Physics, National Academy of Sciences of ... different effects of pH and NaCl on individual O2-binding properties of a and b subunits within liganded tetramer and dimer of human hemo-globin (HbA) were examined in a number of laser time-resolved ... in the absence of NaCl (a) and at a NaClconcentration of 0.5M (b), and 2.0 M (c). Insets shows residuals (a)(b), and (c) from the double exponential fits of the curve (a) (b), and (c), respectively....
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... anextracellular loop of the Ntcp and could represent the sodium ion sensor of sodium- coupled taurocholate trans-port via Ntcp. In order to find out whether and to whatextent this amino acid affects sodium ... circulation of bile acids and therefore alsoparticipate in the homoeostatis of cholesterol. Members of SBATs are located either in apical membranes of ileumenterocytes, kidney tubule cells and bile ... Site-directed mutagenesis of Ntcp (Eur. J. Biochem. 270) 1121Transport of taurocholate by mutants of negatively charged aminoacids, cysteines, and threonines of the rat liver sodium- dependenttaurocholate...
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