titration curve of oxalic acid and sodium hydroxide

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Ngày tải lên: 09/08/2014, 14:21

Ngày tải lên: 13/08/2014, 08:20

... Gymnemic acids inhibit rabbit glycer- aldehyde-3-phosphate dehydrogenase and induce a smearing of its electrophoretic band and dephosphoryla- tion. FEBS Lett 579, 4333–4336. Interaction of gymnemic acid ... average diameter of unbound GA is > 30 nm and that of GA and c-CD complex is 2.2 nm, similar to unbound c-CD, supporting the aggregate property of GA and the inclusion complexation of GA by c-CD. Abbreviations CD, ... presence of 2.0 m M a-CD, b-CD, and c-CD. (B) The concentration dependence of c-CD on NMR spectra of 2.0 m M GA. The ratios of c-CD to GA are indicated. Y. Izutani et al. Interaction of gymnemic acid...

Ngày tải lên: 16/03/2014, 14:20

... Dental Sciences and Surgery, University of Bari, Bari, Italy.  Correspondence to: Prof. F.R. GRASSI, Professor and Dean, Department of Dental Sciences and Surgery - University of Bari, P.zza ... Hyaluronic Acid and Autologous Bone in the Surgical Correction of the Infra-Bone Defects Andrea BALLINI, Stefania CANTORE, Saverio CAPODIFERRO and Felice Roberto GRASSI  Department of Dental ... grafting, 4 males and 5 females, all non smokers, with a mean age of 43,8 years for females, 40,0 years for males and 42 years for all the group, in good health and with a mean depth of the infra-bone...

Ngày tải lên: 03/11/2012, 11:35

... findings of none detectable. The authors also reported 101 ( 38 and 22.1 ( 15.2 µg β-carotene/g FW in extracted samples of aril and mesocarp, respectively, and 16 and 9 µg/g of zeaxanthin and 35 and ... identification of major carotenoids, includes carotenoid profiles, measuring both trans- and cis-isomers of lycopene and β-carotene. We also provide a detailed fatty acid analysis of gac seed and aril, ... linoleic and R-linoleic acids. The aril also contains a significant, but varying, amount of stearic acid and small amounts of cis-vaccenic, myristic, eicosa- 11-enoic, arachidic, and palmitoleic acids. Our...

Ngày tải lên: 09/02/2014, 00:08

... producing transformant and was used for the purification of GALA. Growth of this transformant and subsequent purification of the enzyme were performed as described in Materials and methods. Purity of the preparation ... to facilitate purification of GALA. The enzyme has a molecular mass of 48.5 kDa and a pH optimum between 4 and 4.5. Incubations of ara- binogalactans of potato, onion and soy with GALA resulted initially ... Maldi-TOF MS analysis of the hydrolysis of arabinogalactans by GALA. Three different arabinogalactans were used: potato (A), onion (B), and soy (C). Spectra were taken after 15 min (1) and 2 h (2) of...

Ngày tải lên: 21/02/2014, 01:21

... protection of enzymes and membranes from the detergent effects of excess fatty acids by sequestering them, and (d) modulation of cell growth and differenti- ation by transport of fatty acids to the nucleus ... uptake of fatty acids across the plasma membrane and transport to various subcellular organelles, (b) modulation of the activity of enzymes involved in fatty acid metabolism, (c) protection of enzymes ... consists of four exons of 112, 174, 89 and 141 bp, coding for 22, 59, 30 and 20 amino acids, respectively. The intron ⁄ exon structure of the fabp6 gene from zebrafish is consistent with that of all...

Ngày tải lên: 07/03/2014, 06:20

... alternative route for the production of UDP-glucuronic acid via myoinositol has also been identified and characterized. Synthesis of UDP-glucuronic acid and UDP-xylose T. Oka and Y. Jigami 2646 FEBS Journal ... amounts of the nucleotide sugars, and also discuss the implications of our results in yeast for the study of metabolic regulation in plants. Results Cloning and expression of AtUGD1 and AtUXS3 genes ... inhibition constant (K i )of AtUGD1. Inhibition by UDP-GlcA and UDP-Xyl was measured with various concentrations of UDP-Glc and saturating levels of NAD + . Double-reciprocal plots of the data revealed...

Ngày tải lên: 07/03/2014, 12:20

... ratio of native dimers to monomers, and the loss of H-bonding involving the strands b I of bLG [47], could be promoted in both situations. A CD spectrum assigned to distortions of b-strands showed ... chirality of the environ- ments of the side-chains of aromatic residues (Trp, Tyr and Phe) and disulfide bonds [49]. The two deep peaks found at 286 and 293 nm arise from Trp residues. In the case of ... means of a curve obtained using appro- priate fluorescence standards together with the standard provided with the instrument. The quantum yields of NATA and bLG were determined relative to that of...

Ngày tải lên: 07/03/2014, 15:20

... De-O-acylation of lipid A fractionsledtodecayofspeciesZandYandresultedin increase of signals corresponding to ions of species X (data not shown). The total decrease of mass due to de-O- acylation of phosphorylated ... protons and protons from a, b and c positions of 3-hydroxy fatty acids are marked with; -CH ¼ CH-, -CH 3 ,and- CH 2 -, a, b and c, respectively. CHCl 3 ,DMSOandH 2 O represent signals from solvents and ... 2,3-diamino-2,3-dideoxyglucose (DAG) and a complex set of fatty acids (both ester and amide bound), as described previously [24], but also galacturonic acid and phosphate residues. The presence of GalA was unequivocally...

Ngày tải lên: 07/03/2014, 15:20

... Mendez, Cristina Paz and Ernesto J. Podesta ´ Department of Biochemistry, School of Medicine, University of Buenos Aires Although the role of arachidonic acid (AA) in the regulation of steroidogenesis ... of recombinant CTE-I as antigen dissolved in 0.5 mL of distilled water and mixed with equal volumes of Freund’s complete adjuvant, andthreetimeswith500lg of antigen mixed with equal volumes of ... reports have shown that arachidonic acid (AA) and its metabolites play an essential role in the regulation of steroidogenesis and both the expression and function of StAR [17–26]. Adrenal cholesterol...

Ngày tải lên: 08/03/2014, 09:20

... independent regulation of these two genes and of the accumulation of bryonolic acid and phytosterols. It is noteworthy that the changes in their mRNA levels correlated with the accumulation of the respective ... cycle. These results suggest independent regulation of these two genes and of the accumulation of bryonolic acid and phytosterols. Keywords: bryonolic acid biosynthesis; isomultiflorenol synthase; Luffa ... growth, accumulation of bryonolic acid and total sterol, and mRNA levels of isomultiflorenol synthase and cycloartenol synthase in the cultured Luffa cells. Although...

Ngày tải lên: 08/03/2014, 23:20

... to OPDA and JA synthesis [34]. By contrast, fractions of the unsaturated membrane fatty acid a-linolenic acid and a-linoleic acid are converted randomly and nonenzymatically to a variety of prod- ucts ... variety of abiotic and biotic cues. To deter feeding insects, nematodes and fungal and bacterial pathogens, plants have evolved a plethora of defence strategies. A central player in many of these ... transition and the release of cytochrome c in animal cells [112]. In A549 human lung adenocancer cells, Me-JA operates through the induction of proapoptotic genes of the BCl-2, Bax and Bcl-X families and...

Ngày tải lên: 16/03/2014, 02:20

... to  30% of the initial rate of UBHY-R, whereas the rates of synthesis of DNA and RNA were 70% and 60% of the initial values, respectively. These data suggest that the primary effect of eIF5A ... sites of p414GAL1 and the NdeI–BamHI sites of pET11a. The entire ORF of the site-mutated and truncated eIF5A was sequenced for confirmation of the intended mutation or truncation. Determination of ... mm NAD, 25 lg of BSA, 3 lCi of [ 3 H]spermidine, 0.1 lgof DHS and 1.0 lg of DOHH and clarified BL21(DE3) lysates containing heIF5As (2–5 lg). After incubation for 2 h at 37 °C, an aliquot of the reaction...

Ngày tải lên: 16/03/2014, 06:20

... on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits Sergei V. Lepeshkevich and Boris M. Dzhagarov Institute of Molecular and Atomic Physics, National Academy of Sciences of ... different effects of pH and NaCl on individual O 2 -binding properties of a and b subunits within liganded tetramer and dimer of human hemo- globin (HbA) were examined in a number of laser time-resolved ... in the absence of NaCl (a) and at a NaCl concentration of 0.5 M (b), and 2.0 M (c). Insets shows residuals (a) (b), and (c) from the double exponential fits of the curve (a) (b), and (c), respectively....

Ngày tải lên: 16/03/2014, 14:20

... an extracellular loop of the Ntcp and could represent the sodium ion sensor of sodium- coupled taurocholate trans- port via Ntcp. In order to find out whether and to what extent this amino acid affects sodium ... circulation of bile acids and therefore also participate in the homoeostatis of cholesterol. Members of SBATs are located either in apical membranes of ileum enterocytes, kidney tubule cells and bile ... Site-directed mutagenesis of Ntcp (Eur. J. Biochem. 270) 1121 Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium- dependent taurocholate...

Ngày tải lên: 17/03/2014, 09:20