titration amplification and purification of hsv subheadings  3 1 3 4

... B 30 20 10 Time (h) 16 24 48 16 24 48 n 16 24 48 ODN (2 µg) 30 2C4 15 10 E Lip ODN cont Curc Contr F Dead cells (%) C Dead c cells (%) 0.5 30 15 10 n e 0.5 ODN (µg) 80 60 40 20 10 Cont (µg) 10 ... al (20 03) Targeted inhibition of Stat3 with a 2 5 14 19 20 21 22 23 24 25 26 27 28 29 30 decoy oligonucleotide abrogates head and neck cancer cell growth Proc Natl Acad Sci USA 10 0, 4 13 8 – 41 43 Barton ... (ARC, grant 31 33) and RFBR 06- 04- 4 919 6 References Benekli M, Baer MR, Baumann H & Wetzier M (20 03) Signal transducer and activator of transcription proteins in leukemias Blood 10 1, 2 940 –29 54 Zhong...

Ngày tải lên: 18/02/2014, 08:20

... (mg) Specific Activity (UÆmg )1) Recovery (%) 1: 2: 3: 4: 5: 6: 7: 8: 850 830 800 610 40 0 16 0 97 22 42 00 42 00 790 46 0 90 0.20 0.20 1. 0 1 .3 4. 4 18 24 22 10 0 98 94 72 46 19 11 Cell extract Streptomycin ... Microbiol 16 , 4 01 40 9 19 Mesbah, M., Premachandran, U & Whitman, W.B (19 89) Precise measurement of the G + C content of deoxyribonucleic acid by 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 high-performance ... ( 14 57 bp) of the 16 S rRNA gene of strain 10 d was 96.7% identical with that of Bordetella avium DSM 1 13 3 4T (accession no AF177666), 96.7% identical with that of Bordetella hinzii DSM 49 22T (AF177667),...

Ngày tải lên: 21/02/2014, 01:21

... Dev 17 , 10 09 10 18 34 Szutorisz H, Canzonetta C, Georgiou A, Chow CM, Tora L & Dillon N (2005) Formation of an active Globin locus activation during differentiation 35 36 37 38 39 40 41 42 43 44 ... 11 , 5 13 525 30 Cook PR (19 99) The organization of replication and transcription Science 2 84, 17 90 17 95 31 Riggs AD (19 90) DNA methylation and late replication probably aid cell memory, and type ... 7, 13 9 3 14 01 Elefanty AG, Robb L, Birner R & Begley CG (19 97) Hematopoietic-specific genes are not induced during in vitro differentiation of scl-null embryonic stem cells Blood 90, 1 43 5 14 47 ...

Ngày tải lên: 07/03/2014, 12:20

... 12 1( 24) , 10 7 (10 0), 10 5(20), 91 (33 ), 79(20), 67(52), 53( 22), 41 ( 28) 216 (M1, 13 ) , 14 8 (30 ), 13 3 (10 ), 10 9 (10 ), 10 8 (10 0), 10 5 (12 ), 93( 11 ), 91( 16), 79 (17 ), 77 (15 ) 15 4( M1 ,36 ), 11 1 (47 ), 10 8(50), 93( 36), ... 10 9(80), 95 ( 34 ), 82 (36 ), 69 (10 0), 68( 64) , 67( 61) , 55( 73) , 41 ( 67) 236 (M1,26), 13 5 (28), 12 1 (10 0), 11 9(29), 10 7( 41 ) , 93( 40 ), 91( 29), 69 (33 ), 55 (44 ), 43 ( 28), 41 ( 46 ) 218 (M1,8), 13 6 (49 ), 13 5 ( 71) , 12 1( 24) , ... K4 K5 K6 0 .39 0.60 0.77 1. 49 1. 66 3. 27 0 .33 0.56 0.76 1. 74 1. 94 4. 53 0 .17 0 .32 0.50 1. 02 1. 15 2.22 0.28 0. 54 0.72 2.08 2 .17 6 .49 0 . 34 0. 63 0.75 1. 90 1. 94 5.22 0. 43 0.72 0.80 1. 49 1. 56 2.99 0.58...

Ngày tải lên: 07/03/2014, 21:20

... process and as a goal Human Relations 46 : 17 5 19 2 Ison, R.L (19 93) Changing community attitudes Rangeland Journal 15 : 15 4 16 6 Jiggins, J (19 95) Problem led R&D In Grassland into the 21st Century ... planting 19 61 19 97 Floods, decreasing rainfall, population growth, improved hygiene, new diseases, high incidence, better health care 19 19 Kimiri (smallpox), forced labor 19 41 Plague 19 43 Famine 19 49 ... Foot -and- mouth disease; Exotic trees and crops 19 52 Emergency state, forced into villages 19 57 Land demarcation 19 61 Floods 19 64 Famine, yellow maize 19 70 New ticks species 19 73 New varieties of...

Ngày tải lên: 18/06/2014, 19:20

... 1, 2, 3) Factor Xa cleavage of GP1-MBP 28 60 40 30 20 GP1 17 14 GP2 B 98 62 49 B GP1-MBP MBP 38 28 17 GP1 14 Expression and purification of LASV GP1pMAL-c2x:GP1 Figure gami cells transformed with ... t4 Pa t3 N H S Pa t1 LASV IgG Assay NPbac B 3. 5 1/ 100 3. 0 1/ 200 2.5 O.D Pa t2 0.0 2.0 1. 5 1. 0 0.5 Pa t9 Pa t1 Pa t1 Pa t8 Pa t7 Pa t6 Pa t5 Pa t4 Pa t3 Patient # LASV IgG Assay GP2bac C 3. 5 1/ 100 ... Vaccines 20 04, 3( 2) :1 03- 11 1 McCormick JB: Clinical, epidemiologic, and therapeutic aspects of Lassa fever Med Microbiol Immunol 19 86, 17 5 :1 53- 15 5 McCormick JB: Epidemiology and control of Lassa...

Ngày tải lên: 20/06/2014, 01:20

... 38 8280 38 8887 38 9520 39 019 2 39 13 0 0 39 1 933 V-599 38 41 1 9 38 45 84 38 48 26 38 5 233 38 68 91 38 6990 38 70 23 38 712 4 38 712 9 38 7 13 0 38 7 13 1 38 71 73 38 844 6 39 17 13 3 917 71 39 2 43 8 39 30 84 39 319 8 39 32 73 3 932 82 39 349 2 ... AY 744 680 L 1 14 27 L 116 19 AY 744 678 DQ4 016 90 AY 744 6 84 L 11 43 2 AF 317 645 M 9 31 30 AB 111 085 AF 147 457 AY67 637 0 AY67 636 8 AY67 635 9 AF 533 079 AY 13 5 41 9 AY 145 715 AY 145 716 AY 338 4 93 AY 145 730 AY 145 726 AY 145 718 AY 145 7 23 ... AY496872 AB 111 080 L 1 14 24 AY09 933 6 AY09 933 7 AB 111 0 81 AY702 032 AY 038 605 AY 146 772 AY 146 765 AY 146 767 AY 146 776 AY702 030 AY702 033 DQ3 41 2 09 DQ3 41 2 08 DQ3 41 2 02 DQ3 712 45 DQ177899 DQ177900 DQ367720 DQ177898...

Ngày tải lên: 20/06/2014, 01:20

... http://www.amb-express.com/content /1/ 1/ 13 Page of 4- hydroxyacetophenone monooxygenase from Pseudomonas putida JD1 Appl Environ Microb 75 : 31 06 31 14 doi :10 .11 28/aem.02707-08 Rheinwald JG, Chakrabarty AM, Gunsalus IC (19 73) A ... characterization of the recombinant BaeyerVilliger monooxygenase MekA from Pseudomonas veronii MEK700 Appl Microbiol Biot 77 :12 51 12 60 doi :10 .10 07/s002 53- 007 -12 64- 6 doi :10 .11 86/ 219 1-0855 -1- 13 Cite this ... ketones, 12 served as an example for aliphatic and 13 - 16 for bicyclic ketones Kadow et al AMB Express 2 011 , 1: 13 http://www.amb-express.com/content /1/ 1/ 13 Page of Table conversion of several...

Ngày tải lên: 21/06/2014, 02:20

... decompose (twigs, stalks) 10 cm of material which is easy to decompose (green and fresh) cm of animal manure (if available) A thin layer of soil from the surface of arable land to obtain the micro-organisms ... the boma to save effort moving the manure and used bedding Figure 10 : A boma and compost site (Source: KIOF and HDRA) Like on the drawing, a trench of 30 cm in depth is dug out behind the boma ... next to the trench The bottom of the trench is loosened and a layer of dry vegetation is put on the bottom Then a layer of about 10 cm manure and bedding is thrown out of the boma into the trench...

Ngày tải lên: 02/07/2014, 05:20

... Frequency Years Chapter Reference >18 Periodically 74 Men > 35 Every 235 Every Every Every 1 3 height and weight Cholesterol years Women > 45 years Diabetes >45 or earlier, 33 8 if there are additional ... Alcohol use >18 Periodically 35 6 Vision, hearing >65 Periodically 22, 30 Adult 11 6, 11 7 immunization Tetanus- >18 diptheria (Td) Varicella (VZV) Every 10 years Susceptibles only, >18 Two doses ... factors Pap smearb Within years of onset of sexual years 78 activity or 21 65 Chlamydia Women 18 –25 Every 1 2 16 9 Every 1 2 78, 86 years Mammographya Women > 40 years Colorectal >50 78, 87 cancera...

Ngày tải lên: 06/07/2014, 08:20

... Lung, and Blood Institute: Clinical guidelines on the identification, evaluation, and treatment of overweight and obesity in adults: The evidence report Washington, DC, US Department of Health and ... history, physical examination, and diagnostic tests is used to determine risk and develop a treatment plan (Fig 75 -1) The decision of how aggressively to treat the patient, and which modalities to use, ... initial weight-loss goal of 10 % over months is a realistic target Figure 75 -1 Treatment algorithm This algorithm applies only to the assessment for overweight and obesity and subsequent decisions...

Ngày tải lên: 07/07/2014, 01:20

... Stoecker and Jones Introduction FIGURE 1. 19 Photographs of practical heat pumps (From KIST, Korea.) 37 38 Design and Optimization of Thermal Systems (19 82), Cooper (19 87), and Kreider and Rabl (19 94) ... internal combustion engine (Figure 1. 16a adapted from Reynolds and Perkins, 19 77, and Figure 1. 16b from Moran and Shapiro, 19 96) Introduction 35 FIGURE 1. 17 Typical gas turbine engines for aircrafts ... Ghosh and Mallik, 19 86; Figure 1. 10(b) from Tadmor and Gogos, 19 79.) sketched in Figure 1 .3 Figure 1. 10 shows the schematics for continuous casting, plastic extrusion, glass fiber drawing, and...

Ngày tải lên: 06/08/2014, 15:20

... level is p1 for aggregate supply and aggregate demand curves ASЈ and ADЈ in Figure 3- 1 Increased government spending shifts the aggregate demand curve outward to ADЉ, and the point of equilibrium ... Unemployment, Inflation, and Income 29 Figure 3- 1 the equilibrium point changes from point E1 to E3 Equilibrium output increases from y1 to y2 and the price level falls from p1 to p0 There are two ... basket of approximately 40 0 goods and services 32 PRINCIPLES OF ECONOMICS Table 3. 1 in a given month to the prices that existed during a reference period The producer price index (PPI) and GDP...

Ngày tải lên: 09/08/2014, 19:22

... support of career and technical education in high schools and colleges, and adult basic education and English language acquisition" (U.S Department of Education, Office of Vocational and Adult ... state of Maryland ("Busi­ ness Group," 19 98, p 1B) The Maryland Business Roundtable for Education (MBRT) was formed in 19 92 by 53 companies who came together to support "high standards and rigorous ... virtues of phonics and the failings of whole language Its message has been that the rejection of phonics, in the name of whole language, has resulted in a crisis in literacy in Maryland and the...

Ngày tải lên: 10/08/2014, 00:21

... Parvovirus Vet Pathol 2000, 37 :2 54- 2 63 Zhou et al Virology Journal 2 010 , 7 :36 6 http://www.virologyj.com/content/7 /1 /36 6 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Segales J, Allan GM, Domingo M: ... prevention of porcine parvovirus-induced reproductive failure Vaccine 2006, 24: 54 81- 549 0 doi :10 .11 86 /17 43 - 42 2X-7 -36 6 Cite this article as: Zhou et al.: Production and purification of VP2 protein of porcine ... 19 86, 12 (1) :1- 6 Casal JI: Use of parvovirus-like particles for vaccination andinduction of multiple immune responses Biotechnol Appl Biochem 19 99, 29 : 14 1 -15 0 Casal JI: Parvovirus diagnostic and...

Ngày tải lên: 11/08/2014, 21:21

... 0.50 30 .67 30 .56 30 .62 0.08 0.25 30 .97 30 . 81 30 .89 0 .12 0 .38 30 . 81 30 . 74 30 .77 0.05 0 .16 34 .82 33 .90 34 .36 0.66 1. 91 34 .50 34 .52 34 . 51 0. 01 0. 03 34. 09 33 . 94 34 .02 0 .10 0 . 31 37 .09 37 .44 37 .27 ... 0 .11 0 .46 24 .18 24. 13 24 .15 0. 04 0 .15 24 .12 23. 74 23. 93 0.27 1. 13 27.25 0 .16 0.57 30 .76 0 . 14 0 .45 34 .30 0.25 0. 74 37 . 41 0 .15 0. 41 15,000 27 . 31 0. 04 0 . 14 27 .36 0 .15 0.55 26.97 27 .16 27.07 0. 13 ... (55%) 16 ( 24% ) 11 (16 %) (20%) 10 (25%) Transfusion risk 10 (3% ) (0%) (1% ) (3% ) No identified risk 85(22%) (18 %) 40 (59%) 21 ( 53% ) 0 -1 16 (4% ) (5%) (4% ) (3% ) 2 -4 51 ( 13 % ) ( 23% ) 13 (19 %) (15 %) 5-9 10 -50...

Ngày tải lên: 12/08/2014, 02:20

... 20 01, 15 : 14 93- 15 06 Roberts LM, Smith DC: Ricin: the endoplasmic reticulum connection Toxicon 20 04, 44 :46 9 -47 2 Olsnes S: The history of ricin, abrin and related toxins Toxicon 20 04, 44 :36 1 -37 0 Sandvig ... volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p 24, 30 μg of RTB/p 24, 15 μg of p 24 mixed with μg of CT and, 30 μg of RTB/p 24 mixed with μg of CT adjuvant ... immunized i.p in a volume of 200 μl, with the following doses per group: PBS, 15 μg of p 24, 30 μg of RTB/p 24, 15 μg of p 24 plus complete Freund's adjuvant (CFA), and 30 μg of RTB/p 24 with CFA Mice were...

Ngày tải lên: 12/08/2014, 04:21

... sequences of ca 778 bp from 50 specimens (GenBank accession numbers EU 43 5 8 04, EU 43 5 807, EU 43 5 808, EU 43 5 818 , GQ3 54 41 0 , GQ388 730 – GQ3887 74) The parsimony analysis found 12 5 trees with a length of 211 ... American fauna (S vicaria Walker, 18 49 , S pyrrhosoma Melander and Spuler, 19 17, S melanopoda Duda, 19 26 and S kertezsi Duda, 19 26) 13 1 However, the unexpectedly high level of genetic variability that ... established from ♀ collected from dung on cow farm, 4km NE of Sa Pa town 16 8 [22° 21' 28 .19 "N 1 03 51' 53. 35"E, elevation 12 50m ASL] Collected 15 .VII.2 010 (Ang Y) Diagnostic remarks Sepsis monostigma...

Ngày tải lên: 10/09/2015, 09:32

... 1. 13 . 1 Dpysl3 in the developing nervous system 21 1. 13 . 2 Dpysl3 in adult nervous system 22 1. 13 . 3 Dpysl3 in the peripheral nervous system 22 1. 14 Function of CRMPs 22 1. 14 .1 ... 1. 4 .1 Chemotaxis and migration of microglia 1. 4. 2 Phagocytosis 1. 4 .3 Proliferation v 1. 4. 4 Release of cytokines and reactive oxygen intermediates 1. 5 Activation ... sections 33 2 .3 Cell culture 33 2 .3. 1 Primary microglial culture 33 2 .3. 2 BV-2 microglial cell culture 35 2 .3. 3 Treatment of BV-2 cells 36 2 .4 siRNA mediated...

Ngày tải lên: 12/09/2015, 11:24

... Figure 30 Inductive Non-inductive _ Figure 31 B A Figure 32 A B Figure 33 A B Figure 34 mgb1D WT _ rgs1Dmgb1D magB G183Smgb1D Figure 35 Figure 36 MG 01 0 31 5 MG 010 105 MG09 13 4 Control: Gamma actin MG 039 82 ... G183Smgb1D Figure 35 Figure 36 MG 01 0 31 5 MG 010 105 MG09 13 4 Control: Gamma actin MG 039 82 MG 011 73 MG 01 630 Figure 37 A B ...

Ngày tải lên: 14/09/2015, 09:39