tissues prostate cancer cell lines and cancer tissues
Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... ERa-positive breast cancer cell lines and tumor specimens (A) For breast cancer cell lines, the expression levels of mature miR-22 were determined by quantitative RT-PCR with U6 as an internal standard miR-22 ... regulation of cancer cell proliferation and growth For instance, let-7 can inhibit proliferation of lung and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c ... ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and...
Ngày tải lên: 22/03/2014, 21:20
Evaluation of thymoquinone for cytotoxic activity against human breast cancer cell lines and tumor xenograft in nude mice
... cells (Hussain et al., 2011), DLD-1 human colon cancer cells (El-Najjar et al., 2010) and C4-2B prostate cancer cells (Koka et al., 2010) The effect of TQ on ROS production in breast cancer cells ... cancer (Kumar et al., 2009) and non-small cell lung cancer cells (Chang et al., 2000) Moreover, the invasion and metastasis of breast cancer cells could be inhibited by ligand activation of PPAR-γ ... MCF-7 and MDA-MB-231 breast cancer cells (Colin et al., 2010) In addition, the cytotoxicity of 15d-PGJ2 in 786-O, Caki-2 and ACHN human renal cancer cell lines was mediated in caspasedependent and...
Ngày tải lên: 10/09/2015, 09:09
báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... than 100 molecular and cellular impairments, such as inadequate angiogenesis [7], impaired innervation [8], impaired cellular migration [9] and abnormal keratinocyte activation and differentiation[10] ... Figure 1) Cells from location B are those surgically removed and cells from location C are the cells left behind after surgery One piece of the debrided tissue was sent for routine pathology and other ... the fibroblast cell lines and made them available AK drafted the final version of the manuscript and figure legends MSG revised the figures, added critical content to the discussion and was responsible...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
... suppression of cell growth of human T -cell leukemia virus type Iinfected T -cell lines and primary adult T -cell leukemia cells Int J Cancer 2006, 118:765-772 21 Kunnumakkara AB, Anand P, Aggarwal ... AML cell lines (KG1a and Kasumi-1) and in CD34+ primary AML samples We showed that curcumin selectively induced apoptosis in KG1a and Kasumi1 cell lines, as well as in primary CD34+ AML cells, ... AML cells are 10-15-fold more resistant to DNR than CD34- AML cells [8] CD34+ KG1a and TF-1 AML cell lines are 30-40 fold more resistant to mitoxantrone than more mature HL-60 and U937 cells, and...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... Extra -and intracellular Muc16 expression by MOVCAR cells Extra -and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells were labeled with 618F (grey line) and analyzed for cell surface ... ovarian cancer cell lines MOVCAR 1, 2, 9, and 10–were kindly provided by Dr Denise Connolly (Fox Chase Cancer Center, Philadelphia) and cultured in DMEM supplemented with 10% FBS, 0.2% ITS and 1% ... MOVCAR cell lines showed a clear expression of intracellular Muc16 and only minimal presence of Muc16 on the cell surface (Figs 6a–d) Similar results were obtained with both the 618F and 653F...
Ngày tải lên: 20/06/2014, 07:20
Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
... the cell growth of HTLV-1-infected T -cell lines and primary ATL cells Next we examined the effect of AG490 on the growth of HTLV-1-infected T -cell lines and primary ATL cells HTLV1-infected T -cell ... AG490-treated cells Thus, AG490 is both anti-proliferative and pro-apoptotic in HTLV-1infected T -cell lines Figure and primary ATL cells AG490 reduces cell growth of HTLV-1-infected T -cell lines AG490 ... HTLV-1-infected T -cell lines Figure HTLV-1-infected T -cell lines and primary ATL cells AG490 inhibits constitutive activation of Jak and Stat in AG490 inhibits constitutive activation of Jak and Stat...
Ngày tải lên: 13/08/2014, 09:21
báo cáo hóa học:" Aberrant STYK1 expression in ovarian cancer tissues and cell lines" pdf
... ovarian cancer cell lines SKOV3, CaOv3, and OvCar3 (Fig 2A) Cell lines HS832, OvCar5, OvCar8 and IGROV had no detectable ER transcript While ER expression was weak for most of the cell lines, ... estrogentumor cell Ovarian 2receptors lines express STYK1 and variably express Ovarian tumor cell lines express STYK1 and variably express estrogen receptors HS832 is a benign ovarian cell line and the ... malignant ovarian tissues To investigate the role of estrogen and GPR30 in STYK1 regulation, we treated a benign and several malignant ovarian cancer cell lines with estradiol and G-1 We describe...
Ngày tải lên: 20/06/2014, 07:20
Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... tumor cell lines and compare them to primary cells Cellular lysates were prepared from human foreskin fibroblasts, a primary cell line, and the tumorigenic cell lines Saos, MDAMB231, MCF7, HeLa and ... Quantitation of protein levels in cancer cell lines and primary cells To calculate the number of hCdt1 and h-geminin molecules present per HeLa cell, full-length hCdt1 (HisT7Cdt1) and full-length h-geminin ... sections and their expression levels in primary and normal diploid vs cancer cell lines Our data show a correlation of Cdt1 and geminin expression levels with cell proliferation Materials and methods...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo sinh học: " High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" potx
... for hematological cell lines using cell cycle analysis and cell death measures to determine sensitivity and resistance Cell lines that are early and moderate responders by cell cycle analysis ... from screening cell lines in cellular proliferation assays and from cell cycle analyses Cell lines were classified into one of three categories based on the time when the majority of cells contained ... on both time of response and degree of cell death 20/91 (22%) cell lines were designated sensitive and 39/91 (43%) cell lines were designated resistant (where sensitive and resistant is defined...
Ngày tải lên: 18/06/2014, 22:20
o cáo hóa học:" High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" ppt
... for hematological cell lines using cell cycle analysis and cell death measures to determine sensitivity and resistance Cell lines that are early and moderate responders by cell cycle analysis ... from screening cell lines in cellular proliferation assays and from cell cycle analyses Cell lines were classified into one of three categories based on the time when the majority of cells contained ... on both time of response and degree of cell death 20/91 (22%) cell lines were designated sensitive and 39/91 (43%) cell lines were designated resistant (where sensitive and resistant is defined...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo khoa học: "The combination effect of sodium butyrate and 5-Aza-2''''-deoxycytidine on radiosensitivity in RKO colorectal cancer and MCF-7 breast cancer cell lines" pot
... radiosensitivity Materials and methods Cell line culture and reagents Human colon cancer cell lines RKO (ATCC, USA), breast cancer cell line MCF-7 (KCLB, Korea), and normal colon cell line DDC-112 CoN ... radiosensitivity in colon cancer cell lines Camphausen et al [18] also reported that MS-275, an HDAC inhibitor, increased radiosensitivy in prostate cancer cell lines In this experiment, RKO cell lines showed ... radiosensitivity in head and neck squamous cell carcinoma cell line and Camphausen et al [18] also reported MS-275 could increase radiosensitivity in prostate cancer and glioma cell line Page of (page...
Ngày tải lên: 09/08/2014, 04:21
Báo cáo khoa học: "The Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation" pot
... specified Cell lines and cell culture The prostate cancer cell lines LNCaP (p53 wild type, androgen-dependent, highly differentiated), PC3 (p53-/-, androgen-independent, poorly differentiated), and ... radiation and ErPC3 in prostate cancer cell lines In a first step, the anti-neoplastic effects of ErPC3 and ionizing radiation alone were analyzed in three different prostate cell lines For this, ... Page of 12 Figure Anti-neoplastic effects of ErPC3 and ionizing radiation on prostate cancer cells The prostate cancer cell lines PC3, DU145, and LNCaP, were irradiated (RT) with 2, 5, or 10 Gy...
Ngày tải lên: 09/08/2014, 09:20
báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
... 11 Figure Cells growth curve and MTT analysis for MDA-MB-231 and MCF-7 cells A: cells growth curve analysis for MDA-MB-231 and MCF-7 cells B: MTT analysis for MDA-MB-231 and MCF-7 cell compared ... alpha(ER a ), matrix metalloproteinase 9(MMP-9), cyclinD1, and on cancer cells invasion, proliferation and cell cycle cell in two cell lines were investigated Page of 11 Construction of shRNA expression ... ER-negative human breast cancer cell lines MDA-MB231, and protein levels of MMP-9 and CyclinD1 were down-regulation (P < 0.05) However, in ER alpha-positive breast cancer cells MCF-7, protein expression...
Ngày tải lên: 10/08/2014, 10:21
báo cáo khoa học: " Platinum resistance in breast and ovarian cancer cell lines Niels Eckstein" pptx
... collection of breast cancer cell lines for the study of functionally distinct cancer subtypes Cancer Cell 2006, 10:515-27 Wang D, Lippard SJ: Cellular processing of platinum anticancer drugs Nature ... instance, hepatocellular carcinoma cells with elevated thioredoxin levels are resistant to Cisplatin, but not to the antracyclin Doxorubicin [39] However, bladderand prostate cancer cell lines with ... functional and biochemical changes after establishment of Cisplatin resistance is given Cisplatin resistant breast cancer cells and ovarian cancer cells were compared to their non-resistant parental cells...
Ngày tải lên: 10/08/2014, 10:21
Báo cáo y học: "Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection" ppt
... patients’ prostate cancer tissues and the experimentally infected human tumour cell line DU145 Panel (A) shows the alignment of sequence EU981808 (patient 122-derived) and sequence GU816103 (DU145 cell ... from the patients’ prostate tumour tissues and from the DU145 cells experimentally infected with XMRV It is noteworthy that the prostate tumour tissue sites and the DU145 cell sites were cloned ... Authors’ contributions JAG conceived the study and performed the research JAG, GJT and PK interpreted the data and wrote the paper All authors read and approved the final manuscript Competing interests...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: "Systems biology-defined NF-κB regulons, interacting signal pathways and networks are implicated in the malignant phenotype of head and neck cancer cell lines differing in p53 status" pps
... targets in HNSCC and other cancers with phenotypic and genetic heterogeneity related to p53 status or other abnormalities Materials and methods Cell lines Ten established HNSCC cell lines, UM-SCC ... exons 4-9, and confirmed with western blotting and immunohistochemistry [14] Mutation of p53 was detected in six cell lines, UM-SCC 5, 11B, 22A, 22B, 38 and 46 Four cell lines, UM-SCC 1, 6, and 11A, ... growth factor receptor, PI3K and MEK signal kinases on NF-kappaB and AP-1 activation and IL-8 and VEGF expression in human head and neck squamous cell carcinoma lines Int J Cancer 2002, 99(4):538-548...
Ngày tải lên: 14/08/2014, 08:20
Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... Murray T, Xu J, and Thun MJ Cancer statistics 2007 CA Cancer J Clin 2007; 57: 43-66 Li Y, and Cozzi PJ Targeting uPA/uPAR in prostate cancer Cancer Treat Rev 2007;33(6):521-7 Catalona WJ and Smith ... human primary and metastatic prostate cancer cell lines Biochem Int 1992; 27: 567-577 Stone KR, Mickey DD, Wunderli H, Mickey GH, and Paulson DF Isolation of a human prostate carcinoma cell line ... Shimada M and Nakanishi M DNA damage checkpoints and cancer J Mol Histol 2006; 37: 253-260 51 Damia G and Broggini M Cell cycle checkpoint proteins and cellular response to treatment by anticancer...
Ngày tải lên: 26/10/2012, 09:48
Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... PC1, PC2, and PC3, whereas one LC cell line, 86-2, showed intermediate separation in PC1 and PC3 The three SC cell lines were close to each other Two close LC cell lines and three SC cell lines were ... cell lines, EBC-1 and LK-2, were close in PC1, PC2, and PC3, whereas one SQ cell line, LC-1/sq, showed a distinct, large positive value in PC1 and PC3 Two close AD cell lines and two close SQ cell ... Quantification and validation of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared...
Ngày tải lên: 15/02/2014, 04:20