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... (generating 5.8S rRNA) or pBluescript (nonspecific RNA) in the presence of 10 mm NTPs. UV cross-linking was performed by placing RNA–protein complexes on ice and irradiation for 15 min at 260 nm using ... Nop53p binds 5.8S rRNA. UV cross-linking was performed after incubation of 1 pmol of in vitro transcribed, uniformly labeled 5.8S rRNA with increasing amounts of His–Nop53p or bovine serum albumin ... Analyzing protein–protein interactions using two-hybrid system. Methods Enzymol 254, 241–263. 42 Zanchin NIT & Goldfarb DS (1999) Nip7p interacts with Nop8p, an essential nucleolar protein required...

Ngày tải lên: 20/02/2014, 01:20

... phenomena that are in- volved in determining answerhood. Our work suggests that considerable gains in performance can be obtained by incorporating TE during both answer processing and passage re- trieval. ... in a text can be inferred from the information expressed in another text, can be performed in four ways. We can try to (1) derive linguistic information from the pair of texts, and cast the inference ... corresponding entities, predicates, or phrases found in a pair of texts. In this section, we show that by using a machine learning-based classifier which combines lexico-semantic infor- mation...

Ngày tải lên: 08/03/2014, 02:21

Ngày tải lên: 19/06/2014, 10:20

Ngày tải lên: 22/06/2014, 00:20

... kiện và hoàn cảnh giảng dạy thực tế. Đối với học sinh THCS hiện nay, tiếng anh đã trở lên phổ biến và thầy cô giáo cũng đã cung cấp cho học sinh một vốn kiến thức mới, biết được các phương pháp ... xác và trôi chảy những điều cần thông tin. Tuỳ theo mục đích yêu cầu của bài học mà người dạy có thể lựa chọn một số kĩ thuật thích hợp để xây dựng các sinh hoạt học tập trong lớp và các bài ... Structured Interview là một ví dụ của sự điều chỉnh vừa nêu. Trong bài học này người học phỏng vấn lẫn nhau, sử dụng cấu trúc ngữ pháp đã học, nhưng họ trả lời với những thông tin có thật mà...

Ngày tải lên: 02/10/2012, 14:10

... and learning and (3) advantages of using computers in reading ESP teaching and learning. I.2.3.1.1.Model of teaching ESP reading with computers Some models of using computers in teaching FL can ... of computers for teaching and learning (in general) and teaching and learning FL (in particular). As far as this study is concerned, certain applications of computers in teaching and learning ... following main methods to do the research:  Consulting books, journals and newspapers on applying IT in teaching and learning foreign languages or ESP;  Searching information on the Internet...

Ngày tải lên: 07/11/2012, 14:31

... lựa chọn định dạng phong phú - Match Destination Formatting (chuyển định dạng dữ liệu vừa dán giống với định dạng của cả văn bản) và Keep Source Formatting (giữ nguyên định dạng của đoạn dữ liệu ... bản. Xoá thông tin cá nhân Các tệp tin văn bản của MS Word có chứa những thông tin ẩn có thể tiết lộ thông tin về bạn. Microsoft cũng đã cho ra công cụ xoá bỏ những thông tin ẩn này Remove ... MS Office chính là Format Painter – có hình dáng như chiếc chổi sơn trên thanh công cụ chuẩn (Standard toolbar). Nếu chưa có bạn có thể vào Tool | Commands, tìm đến mục Format và kéo biểu tượng...

Ngày tải lên: 09/09/2013, 17:10

Ngày tải lên: 07/12/2013, 11:41

Ngày tải lên: 27/12/2013, 20:26

... and police for everything from underage drinking to hit and runs. Be careful what you post in Facebook just like everywhere else online. Effective Internet Presence: Now required for success in business ... the business requirements.” He has been on the Internet forever and using email since well before the term ‘email’ was ever coined. Ted has written several books on the Internet and Business, ... developing an Effective Internet Presence, including upcoming teleseminars and hopefully another ebook or two on the subject. More information about Ted is available via Google, or: Internet Business...

Ngày tải lên: 14/02/2014, 04:20

... Protein A Agarose ⁄ Salmon Sperm DNA incubation for no more than 1 h. Beads were washed twice for 15 min in lysis buffer containing 150 m m NaCl, followed by a 15-min wash in lysis buffer containing 500 ... reduced in a strain lacking both the Swi1 and Snf5 activator-binding domains. Recruitment of the SWI ⁄ SNF complex via activator interactions with these activator-binding domains is thus critical for ... promoters under inducing condi- tions (galactose, 30 min) in the wild-type strain (black bars, Wt), strain YPP310 (light grey bars, DDABDswi1 + snf5) lacking both activator-binding domains (SWI1D329-655,...

Ngày tải lên: 07/03/2014, 00:20

... of the gastrin–transferrin inter- action is still unknown. Knowledge of the regions of transferrin required for the binding of gastrin, and of the regions in gastrin required for the interaction ... is required for the Ggly–trans- ferrin interaction, to define the domains ⁄ residues of Ggly involved in the interaction (using Ggly mutants), and, finally, to determine the regions of transferrin required ... connection between gastrins and iron homeostasis was first provided in a search for gastrin- binding proteins in porcine gastric mucosa [14]. An interaction between Gamide and transferrin was identi- fied...

Ngày tải lên: 07/03/2014, 02:20

... project into mini projects. In the kitchen, for example, mini projects might include countertop, under the sink, cutlery drawers, pantry, refrigerator/freezer, recipes, and coupons. For 5 to 15 minutes ... organizing your entire house. Start by gathering and getting things to the rooms where they belong. In the second pass, you can begin to organize the contents of each room. can learn by reading ... following people: My colleagues in the National Association of Professional Organizers, for putting the professional in organizing. Everyone at Storey Publishing who has had a hand in creating...

Ngày tải lên: 15/03/2014, 23:18

... have found tiny amounts of things like heart medicine, hormones and pain killers in their drinking water. Here in Santa Clara County, scientists detected eight different drugs, including ibuprofen ... NEVINS: “Our study is mainly focusing on not the population-level impacts but how we can use the birds as samplers of the marine environment.” Nevins is using the fulmar stomachs as indicators ... Sunday morning, in late April at the Berkeley Marina dozens of anglers are casting their fishing rods over the pier, hoping for a bite. BERKELEY ANGLERS: Well we’re doing some halibut fishing… Yeah...

Ngày tải lên: 15/03/2014, 23:20

... obtained, as discussed in detail below, indicate a crucial role for sulfur–sulfur interactions in defining the catalytically competent binding mode of CoA in the active site. The pantetheine binding ... for increasing the affinity of binding, as well as the solub- ility of the substrate. Interestingly, the side chain of Ser247 points away from SPP, as previously seen in unliganded thiolase; in the ... cor- responding distance for the CoA complex is 3.9 A ˚ (Table 2). The pantetheine moiety of SPP binds in the pant- etheine binding tunnel in a similar (but not identical) way to that seen for CoA...

Ngày tải lên: 16/03/2014, 04:20

... lgÆmL )1 spectinomycin. For thylakoid isolation and physiological measurements, wild-type and transformed plants (originating from two independent transplastomic lines) were planted in compost and kept in ... was explained by a displacement of this domain. Thus, PSI-J does not appear to participate directly in binding of Pc or Cyt c 6 , but plays a role in maintaining a precise recognition site for the ... the luminal side of PSI involving PSI-F and PSI-N. The absence of PSI-J might affect the conformation of PSI-F, which, in turn, changes the binding of PSI-N. PSI-F provides part of the Pc-binding...

Ngày tải lên: 16/03/2014, 11:20

... were grown in Eagle’s Minimum Essential medium (Nissui Pharmaceutical) containing 10% bovine serum in a CO 2 incubator at 37 °C. Screening for Hsp modulators A reporter plasmid containing the Hsp105 ... kept at 4 °Cfor 15 min, and vortexed for 15 min at 4 °C. After centrifuga- tion at 50 000 g for 5 min, aliquots of the supernatant (15 lg protein) were incubated in 25 lL buffer containing 10 m M Tris/HCl ... for 15 min, Luc protein in the supernatant (s) and pellet (p) fractions were detected by Western blotting using anti-Luc Ig [upper panels in (b)]. (C) The structure of p173OR plasmid containing...

Ngày tải lên: 17/03/2014, 10:20

... impli- cated in substrate binding, are shown in Fig. 3 to be within hydrogen bonding distance from the substrate. As energy minimization only allows for limited con- formational sampling, we also ... monitored by the increase in emission at 400 nm over time using k excitation = 325 nm. The mcaGnRH 1–9 substrate was monitored by HPLC (Agilent 1100) using the increase in peak area for the emission ... regions lining the substrate-binding site allow the enzyme to conform to substrates of varying structure. This study describes mutant forms of thimet oligopeptidase in which Gly or Tyr residues in...

Ngày tải lên: 23/03/2014, 06:20

... plated in media containing Geneticin to select for cell containing vector DNA. After three weeks, the number of cells were determined by fixing cells and staining with dye and quantified in (H). ... media containing Geneticin to select for cell containing vector DNA (as in Fig. 5A,G). After three weeks, the number of cells was determined by fixing cells and staining with dye. Individual colonies ... structure to SEP53 containing an N-terminal EF-hand calcium-binding domain and multiple C-terminal amino acid repeat sequences. Using a protein BLAST search, several proteins on the 1q21 locus...

Ngày tải lên: 23/03/2014, 10:21