three dimensional structures of pectate and pectin lyases

... of solved three- dimensional structures of family 11 xylanases are now available for both mesophiles and thermophiles, we made a detailed comparison of the structures of these enzymes Twelve structures ... resembles a ¨ ¨ right hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms a thumb, and a loop between the B6 and B9 strands forms a cord (Fig ... C-terminal b-strand A4 gives additional hydrogen bonding with b-strand A5 and the extension of b-strand B3 interacts with a b-strand B4 BAX also has a longer C-terminal b-strand A4 and a short additional...

Ngày tải lên: 20/02/2014, 23:20

... Copyright Computers and Structures, Inc., 1978-2002 The CSI Logo is a registered trademark of Computers and Structures, Inc SAP2000 is a registered trademark of Computers and Structures, Inc Windows ... and all associated documentation are proprietary and copyrighted products Worldwide rights of ownership rest with Computers and Structures, Inc Unlicensed use of the program or reproduction of ... authorization from Computers and Structures, Inc., is explicitly prohibited Further information and copies of this documentation may be obtained from: Computers and Structures, Inc 1995 University...

Ngày tải lên: 06/09/2012, 15:56

... unmatched in terms of ease -of- use and productivity Creation and modification of the model, execution of the analysis, and checking and optimization of the design, and production of the output are ... Copyright Computers and Structures, Inc., 1978–2006 The CSI Logo is a registered trademark of Computers and Structures, Inc SAP2000 is a registered trademark of Computers and Structures, Inc Windows ... and all associated documentation are proprietary and copyrighted products Worldwide rights of ownership rest with Computers and Structures, Inc Unlicensed use of the program or reproduction of...

Ngày tải lên: 01/04/2014, 03:20

... factor of 18.8% and an Rfree of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2) The resulting model is depicted in Fig The Ca backbones of the ... the three invariant catalytic residues (Fig 1) All a-helices and b-strands are labeled Fig B-factor plots of the main chains of the two molecules of CDase in the asymmetric unit The averages of ... group As the polypeptide superposition of Fig places the three catalytic residues of CDase (Fig 1) within less than ˚ A of the positions of those of TVA-II, and as the active centers closely resemble...

Ngày tải lên: 08/03/2014, 02:20

... space group of the 1Q9H crystal, were found The best solutions had correlation coefficients of 56.1% and 55.3%, and R-factors of 40.0% and 40.9% for 1CEL and 1GPI, respectively Refinement of the models ... was 1.8°, the standard deviation of the hydrogen bond energies was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant ... in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops extending from the...

Ngày tải lên: 23/03/2014, 13:20

... mixture of chloroform and methanol (2:1 [v/v]) After extraction, 0.2 volumes of 0.9% NaCl solution were added and the chloroform-containing phase was collected After evaporation of the chloroform and ... be used both for visualization and for quantification In this study we provide an example of 3-D reconstruction of nuclei, of chromatin distribution and of sites of lipid peroxidation in cells ... in (a) and (b) of Fig Discussion In this study we present a versatile, simple and suitable approach to 3-D reconstruction of intracellular QD location and of changes in nuclear morphology and lipid...

Ngày tải lên: 11/08/2014, 00:22

... evaluate head, rib cage, and pelvic posture as three rotations and two translations or five degrees of freedom (DoF) In fact for 11 out of 15 variables (a total of 44 Intra- and Inter-examiner ICCs), ... measurements for each variable, three examiners provided three pairs of differences of first and second measurements The more liberal MADOM averaged the three pairs of differences within the same ... methodology provided three sets of intra-examiner data and three sets of inter-examiner data Inter- and intraclass correlation coefficients (ICCs) were calculated for each of 15 variables (measurement...

Ngày tải lên: 13/08/2014, 14:20

... heaven and earth And the earth was void and empty, and darkness was upon the face of the deep; and the spirit of God moved over the waters And God said: Be light made And light was made And God ... derived from nature and society and appear to be essential for the social activities of man, e.g actor and action; the bearer of a quality or of a state and the state; action and an object resulting ... process of progressive accumulation, as well as the dynamism of all other elements of utterance (semantic and grammatical too), arising out of the semantic and formal 27 you tension and of expectation...

Ngày tải lên: 24/02/2014, 18:20

... G-tracts of one strand and only the 3¢-end G-tract of the other strand [30] (Fig 3D) The core of this structure, called the (3 + 1) core, has three strands oriented in one direction and one strand ... conformations of guanines along two consecutive G-tracts are 5¢-syn-anti-anti-3¢ and 5¢-syn-syn-anti-3¢ for one strand and 5¢-syn-syn-anti-3¢ and 5¢-syn-anti-anti-3¢ for the other strand of the dimer; ... core (Fig 2A–D): (a) four strands are oriented in the same direction (designated a parallel-stranded core) (Fig 2A); (b) three strands are oriented in one direction and the fourth in the opposite...

Ngày tải lên: 06/03/2014, 09:22

... refinement were 22.5% and 28.0%, respectively Overall description of the structure The three- dimensional structures of NiV and HeV ˚ two-helix are very similar, with an rmsd of 1.4 A for all Ca ... effects of HR2 on viral fusion and formation of the fusion core structure The results also show that the HR2 proteins of NiV and HeV are functionally and structurally interchangeable, and this ... constructs of both NiV and HeV F proteins A schematic diagram of NiV and HeV F proteins with the location of structurally significant domains is given The listed sequences of HR1 (137–178) and HR2...

Ngày tải lên: 08/03/2014, 08:20

... positions of androstenone (not shown) are undistinguishable from those of androstenol In (A) and (B): view of the lipocalin scaffold and the bound ligand In (A) and (B) the rings of the ligand are ... respectively, in isoform A of SAL, the structure of which is described here, and are replaced in isoform B by Ala and Val, respectively Replacement of residues 43 and 75 with those of isoform B in the ... Fig Views of the model of the complex of androstenol bound in SAL, with in (A) and (C) the ligand in position ÔinÕ, with the OH pointing inside the cavity and, in (B) and (D) the ligand in position...

Ngày tải lên: 17/03/2014, 17:20

... side-chain oxygens of Glu309 on M4 and of Asn796 and Asp800 on M6 In our PMA1_NEUCR model, these residues correspond to Ile331, Ile332, Val334, and Val336 on M4 and Ala726 and Asp730 on M6 Alanine-scanning ... located between Val289 and Ile293 in the N-terminal end of M3, and Trp756 and Gly757 in the cytoplasmic loop connecting M6 and M7 As shown by mutagenesis in yeast, mutation of Val289 to Phe resulted ... the center of the b-strands forming the Rossman fold and leads to the membrane domain at the top of M4 and M5 (see Fig 4, cavities numbered 1–6) As could be expected from the high level of sequence...

Ngày tải lên: 23/03/2014, 21:20

... abstraction of the proton from the Ca atom of the substrate Further structural and kinetic studies with site mutants of residues at the active site and determination of structures of ligand and inhibitor ... structure had 93.1% and 6.3% of the residues in the favoured and additionally allowed regions and two residues (Ile111 and His319) in the disallowed region Statistics of the Ramachandran plot obtained ... 1.32 A Superposition of large and small domains of StDSD with the corresponding domains of SeMetDSD ˚ results in rmsd values of 0.53 and 1.64 A, respectively 2882 A number of local conformational...

Ngày tải lên: 28/03/2014, 22:20

... open form Results and Discussion Overall structures of TvuCMBP–ligand complexes The crystal structures of TvuCMBP liganded with a-CD, b-CD and G4 have been determined at 1.7, 1.8 ˚ and 1.55 A resolutions, ... (a) and (b)] of G4 (A), the structurally shared three glucose residues (labeled 2, 3, and 4) of CDs (B), and the structurally nonconserved glucose residues of a-CD (labeled 5, 6, and 1) (C) and ... Ile279, and Val281, are found at the termini of hinge-1 and hinge-2 (Fig 6A,B) The primary structures of hinge-1 and hinge-2 of TvuCMBP and related proteins [18–23,35] were aligned on the basis of...

Ngày tải lên: 29/03/2014, 23:20

... for the three- dimensional near-field optical and polarization-dependent measurement of bowtie nano-aperture are presented We employ contact and CHS mode NSOM to investigate the three- dimensional ... size of 260 nm × 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions ... x-direction (A) and ydirection (B) Note the different intensity scales in the two figures Three- dimensional mapping of the near-field intensity can be acquired by turning off the feedback and scanning...

Ngày tải lên: 06/05/2014, 08:54

... for the three- dimensional near-field optical and polarization-dependent measurement of bowtie nano-aperture are presented We employ contact and CHS mode NSOM to investigate the three- dimensional ... size of 260 nm × 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions ... x-direction (A) and ydirection (B) Note the different intensity scales in the two figures Three- dimensional mapping of the near-field intensity can be acquired by turning off the feedback and scanning...

Ngày tải lên: 06/05/2014, 08:58

... Cn the set of the polyominoes of volume n and by C the set of all polyominoes The area A(c) of a polyomino c is the number of two dimensional unit squares belonging to the boundary of only one ... of the parallelepiped) References G.E Andrews, The theory of partitions, Encyclopedia of Mathematics and its Applications, Addison– Wesley, 1976 G Ben Arous and R Cerf, Metastability of the three ... area |c| of the polyomino c is the number of its unit squares We denote by Cn the set of all the polyominoes of area n The perimeter P (c) of a polyomino c is the number of unit edges of the dual...

Ngày tải lên: 07/08/2014, 06:20

... the radius of the nanoparticle, l is the incident wavelength, n is the refractive index of environmental medium and εr and εi are the real and imaginary parts of the dielectric constant of the nanoparticle, ... movement and uptake of AuNPs for 45 nm and 70 nm AuNPs Many 45-nm AuNPs are moved and then uptaken by cells, while many 75-nm-AuNPs only moved to the apical surface of the cells Wang et al Journal of ... the binding energy between ligands and receptors This energy refers to the amount of ligand-receptor interaction and the diffusion kinetics for the recruitment of receptors to the binding site...

Ngày tải lên: 11/08/2014, 00:22

... 3: “The worm” presented in three- dimensional offline reconstruction of the two -dimensional transesophageal data Three- dimensional offline reconstruction of the two -dimensional transesophageal ... modified mid-esophageal aortic short-axis view of two dimensional transesophageal echocardiography (80°); [C/D] three- dimensional offline reconstruction of transesophageal echocardiography (80°); ... echocardiography PM, TB and MP performed the transthoracal and transesophageal echocardiography PM, TB, MP and AZ analyzed the images of the offline reconstruction AM and HT have been involved...

Ngày tải lên: 11/08/2014, 02:22

... gender and family status but not for age and study centre In the case of the rawscores of the SDQ and the SCQ scales, we expected effects of age, status, gender, and informant [24,26,47], and probably ... interpretation of the results must bear in mind to which of three categories the data belong These categories reflect the nature and degree of standardisation applied to the data and lead to different ... their mean overall ratings and in the differential perception of probands and siblings, but not in the ratings for boys and girls (Table 3) Effects of the informant and the interactions Intelligence...

Ngày tải lên: 11/08/2014, 15:22