thesis cover amp title page
Synthesis of mannoside glycans of phosphatidylinositol mannosides (PIMs
... Method; Synthesis of a Model Trimannoside 13 3.5 Synthesis of Mannoside Glycan Units of PIMs 15 3.5.1 Synthesis of Dimannoside of PIMs 15 3.5.2 Iterative Glycosylation: Synthesis ... A: Synthesis of Mannoside Glycans of Phosphatidylinositol Mannosides (PIMs) 3.5 Synthesis of Mannoside Glycan Units of PIMs 3.5.1 Synthesis of Dimannoside of PIMs The planned glycan synthesis ... Followed by Macrocyclization 92 2.3.1 Synthesis of Allylic Alcohol Building Block 93 2.3.2 Synthesis of Alkynal Synthons 93 2.3.3 Synthesis of RCM Precursor 94 2.3.4...
Ngày tải lên: 10/09/2015, 15:50
Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot
... glycopeptide purification and/or sample enrichment The data are consistent with previous findings, which indicated that the mobility of LC2, but not of LC1, on SDS -PAGE was shifted noticeably with ... sample was digested with sequencing-grade modified trypsin (Promega Corp.), and the resulting glycopeptide and peptide mixtures were analysed directly by LC-MS/MS For N-glycan analysis, the sample ... true positives Western blotting analyses of the glycan epitopes Samples of lg of RVV-X and lg of BSA were analysed by 8% SDS -PAGE under reducing conditions Appropriate amounts of Lex/a- and SLex/a-conjugated...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
... Peptide synthesis We carried out both manual and automated syntheses as described below, using an Fmoc solid-phase strategy with the amino acid derivatives shown in Scheme The manual synthesis was ... the solid-phase glycopeptide synthesis The amino acids are numbered starting from the amino terminal according to accepted nomenclature As solid-phase peptide synthesis starts from the carboxy ... efficiency of the coupling reactions In order to scale up the automated synthesis to the same level as the manual synthesis (280 lmol) we carried out nine separate automated syntheses Each dried...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein pot
... by cochromatography with a candidate reference on the columns in the positive-ion mode Purified samples were dissolved in 50% aqueous methanol solution containing 0.2% formic acid, and loaded into ... dissociation (CID) MS/MS sequencing using a dedicated MALDI-QTOF Ultima instrument (Micromass) Samples in acetonitrile were mixed : with a-cyano-4-cinnamic acid matrix (in acetonitrile/ 0.1% trifluoroacetic ... using a Hewlett-Packard Gas Chromatograph 6890 connected to a HP 5973 Mass Selective Detector Sample was dissolved in hexane before splitless injection into an HP-5MS fused silica capillary column...
Ngày tải lên: 23/03/2014, 17:22
Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
... four urine samples from control individuals as well as seven urine samples, five amniotic fluid samples and two ascitic fluid samples from galactosialidosis patients In the four urine samples of healthy ... products of complex-type N-sialyloligosaccharides were found in all urine samples, amniotic fluid samples and ascitic fluid samples (see Fig 2, n1–n6) [12] A varying number of isomers were detected ... 0.1 0.3 16.4 Mean for amniotic Mean and Mean ascetic for all for urine samples Amf1 Amf2 Amf3 Amf4 Amf5 Asf1 Asf2 samples samples Table Oligosaccharides observed in various body fluids of galactosialidosis...
Ngày tải lên: 29/03/2014, 09:20
Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
... elongation [18] This situation was resolved by total synthesis of a complete LEC14 N-glycan The availability of material derived from chemical synthesis not only unambiguously confirmed this particular ... N-acetylglucosamine (GlcNAc) or a core-fucose (Fuc) residue have lent support to the validity of our hypothesis [12–14] These two substitutions act like switches on ligand properties With these data in ... the pergalactosylated LEC14 dodecasaccharide (Fig 1, compound 7), by combining chemoenzymatic synthesis with biochemical ⁄ cell biological techniques In order to imitate the natural presentation...
Ngày tải lên: 30/03/2014, 16:20
New Insight into IELTS Student book with answers 2008 Answers - Part 5 out of 5.pdf
Ngày tải lên: 07/08/2012, 11:48
History Of Modern Biotechnology II
... Transport Mechanisms Synthesis of the Cell Wall: Chitin Synthesis of the Cell Wall: Glucan Synthesis of the Cell Wall: the Structure ... cite many other examples from the literature showing the relation between cytoskeletal components and vesicle transport 4.2.1.3 Synthesis of the Cell Wall: Chitin Chitin synthesis occurs exclusively ... ADIA 9 The Morphology of Filamentous Fungi Closely related to ADIA is automated sampling, which allows on-line sampling and measurement of many interesting properties, including morphology This...
Ngày tải lên: 08/08/2012, 09:49
History of Modern Biotechnology I - Springer
... antibiotics – biosynthesis and functions Walter de Gruyter, Berlin, p 389 241 Piret JM (1981) PhD Thesis, Massachusetts Institute of Technology, Cambridge, USA 242 Daher E, (1983) M.S Thesis, Massachusetts ... biosynthesis as described below Discovery of glucose isomerase is a contribution originated from Japan, leading to worldwide application in the sugar industry In 1965, Sato and Tsumura [9] discovered ... often amphiphilic, killing microbes by permeabilizing cell membranes They are produced by humans, rats, rabbits, guinea pigs, mice, cattle, pigs, crabs, insects, sheep, frogs and other primitive amphibians,...
Ngày tải lên: 08/08/2012, 09:52
09 Physical and Chemical Characteristics of DDGS revisions.
... 2001 where representative samples of DDGS (4.5 kg) were obtained from 16 ethanol plants in Minnesota, South Dakota and Missouri From these samples, a 200 gram subsample of DDGS from each plant ... conducted by researchers at the University of Minnesota in 2004 (34 samples from ethanol plants in 11 different states) and 2005 (35 samples) As shown in Tables and 5, average particle ranges 08 - ... studied a limited number of DDGS samples under laboratory conditions They reported that relative humidity greater than 60% seemed to reduce flowability of a DDGS sample, which is likely due to the...
Ngày tải lên: 08/08/2012, 10:03
Use of DDGS in Swine Diets
... total of 48 mid-belly samples were collected from each dietary treatment group, with equal numbers of barrows (n=12) and gilts (n=12) from each farm From the 48 mid-belly samples, a visual color ... scores All belly fat samples were then analyzed to determine complete fatty acid profiles Iodine value and mean melting point were calculated using fatty acid data from each sample As shown in Table ... respective dietary treatments Air samples were collected weekly from the headspace above each simulated pit and analyzed for hydrogen sulfide (H2S) and ammonia (NH3) Air samples collected during weeks...
Ngày tải lên: 08/08/2012, 10:03
