thermal properties of solid matter

... event succeeded through the efforts of many people Special thanks are due to the whole staff of volunteers of students of the physics department of the University of L’Aquila (Patrizia De Marco, ... orbitals (HOMO–LUMO) of benzene This is a result of the confinement of the π electrons from the delocalized state of pristine graphene to small aromatic domain of the size of a benzene ring, that ... Jantzen of Springer Of ce for their helpful assistance during the preparation of this special volume The Editors and Chairs of GraphITA Vittorio Morandi Luca Ottaviano Contents Study of Graphene...

Ngày tải lên: 24/03/2014, 01:20

... different physicochemical properties It is also broadly compatible with the work of Ikehara and colleagues [10,11] and of Rodin and Rodin [12] on the origin and evolution of the genetic code However, ... Ikehara K: Pseudo-Replication of [GADV]-proteins and origin of life Int J Mol Sci 2009, 10:1525-1537 Rodin SN, Rodin AS: On the origin of the genetic code: signatures of its primordial complementarity ... part in early evolution This approach to the study of protein folding has no connection at all with mRNA structure or the distinctive properties of codon bases It seems likely that Berezovsky and...

Ngày tải lên: 13/08/2014, 16:20

... prodomain of SVMPs been detected in the venom [11] (Serrano, unpublished results), suggesting that, in fact, the bulk of the proteolytic processing of the proforms of the SVMPs occurs in advance of ... the N-terminal regions of the disintegrin- like domains of these two proteins, although the C-terminal regions of the domains are probably similar to those of the rest of the class Whether the ... Interaction of the cysteine-rich domain of snake venom metalloproteinases with the A1 domain of von Willebrand factor promotes site-specific proteolysis of von Willebrand factor and inhibition of von...

Ngày tải lên: 18/02/2014, 18:20

... the reduction potential of the redox buffer compared with that of PIP, hence the disulfide stability of HPI is lower than that of PIP Physical and chemical properties of the HPI disulfide isomers ... addition of thiol reagents than those of PIP, indicating that the C-peptide of HPI reduces the stability of the disulfide bonds more than that of PIP The reduced disulfide bond stability of HPI may ... molecular mass of the disulfide isomers of HPI and the enzyme-digested fragments were measured by ESI MS The molecular mass of the mixture of scrambled isomers was measured by MALDI-TOF MS CD studies...

Ngày tải lên: 19/02/2014, 12:20

... biochemical outcome of these point mutations Results The effect of chaperonin on the ability of the mutant proteins to fold For a number of other MCAD point mutations, co-overexpression of the GroELS ... standard deviation of the mean result) (B) Western blot analysis of native PAGE gels, indicative of relative amounts of soluble tetramer No MCAD activity was detected in lysates of cells expressing ... coefficient of the bound cofactor in the case of the mutants) [15] The peak absorbance ratios showed first of all that the wild-type enzyme as purified here (ratio ¼ 7.2) is somewhat depleted of FAD...

Ngày tải lên: 20/02/2014, 02:21

... efficiency of the oxidative folding process Recently, on the basis of the physical properties of PDI, a variety of CXXC peptides have been synthesized and assayed [27] The active site of PDI has ... oxidative folding of these enzymes with a concomitant recovery of their catalytic activity Indeed, after 22 h of incubation in the presence of SsCSC and PfCGC, the enzymatic activity of SsMTAPII and ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 30 Lyles MM & Gilbert HF (1991) Catalysis of the oxidative folding of ribonuclease...

Ngày tải lên: 07/03/2014, 00:20

... [15] Analysis of a structural model of AmFd suggests that a combination of additional surface ion pairs, the zinc cofactor, and an efficiently packed core govern the high stability of this protein ... (TM of 50 °C, pH 7; DGU of 11 kJÆmol)1, pH 7, 20 °C) [44] This difference in thermodynamic stability correlates with two orders of magnitude slower unfolding of TmCsp as compared to unfolding of ... absorption (cofactor environment) For time-resolved folding investigations, stopped-flow mixing instruments are often necessary, which have a mixing dead time of 1–2 ms Experimental analyses of the...

Ngày tải lên: 07/03/2014, 05:20

... drastically decreases the on and off rates of substrate from BiP One of the two nucleotide exchange factors then mediates the release of ADP, allowing the binding of ATP, which opens the lid to ... inhibited, but the translation of a subset of mRNAs is enhanced One of these encodes the transcription factor ATF4 [209] ATF4 promotes the transcription of a specific set of UPR target genes, distinct ... appropriate stoichiometry of the subunits in a hetero-oligomeric complex is important for correct functioning of the complex The regulation of the expression of only one of the subunits provides...

Ngày tải lên: 07/03/2014, 06:20

... consequence of the presence of unfolded or incorrectly folded human IRP1 This implies that formation of inclusion bodies competes with assembly of the [4Fe)4S] cluster in IRP1 In addition, degradation of ... Formation of aggregates is not a general feature of the heterologous production of human aconitases in yeast mitochondria, but a particular property of human IRP1 The active participation of mitochondrial ... accumulation of transition metals seemed to correlate with the formation of m-hIRP1 aggregates Of particular interest, iron was found in almost equal proportions (within 15%) in mitochondria of yeast...

Ngày tải lên: 07/03/2014, 10:20

... chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individual domains of human GCPII (as ... 44/750 and 1/750 variants A decrease of more than 80-fold in the secretion of recombinant protein was associated with the deletion of the C-terminal part(s) of the protein, even though the intracellular ... were observed in the cell lysates at any of the time-points (data not shown) Analysis of carboxypeptidase activities of the individual truncated mutants of GCPII The carboxypeptidase activities...

Ngày tải lên: 07/03/2014, 15:20

... van’t Hoff analysis are close to %5000 JÆK)1Æmol)1 [58,59] It is not entirely clear whether burial of 66–71% of the hydrophobic surface area of the native state should account for all of the ... capacity and entropy of formation of the TSE for folding (U-à) Reported errors reflect the quality of the nonlinear least squares fit to the experimental data We have used the F45W mutant of yeast ubiquitin ... structural analysis of F45W mutants of the yeast protein confirms this We have explored the context-dependent effects of proline on ubiquitin stability by introducing a number of substitutions at...

Ngày tải lên: 07/03/2014, 16:20

... the value of h222 the a helical content of the native protein is estimated to Table Effect of the variation of the protein concentration in GdnHCl induced equilibrium unfolding transition of s-MDH ... band pattern of different molecular species with increasing time Figure 8B reflects the kinetics of reassociation of s-MDH as estimated from the relative peak areas of the scanned profile of the individual ... 80% of total decrease of F340 kmax shifts from 339.6 to 347 nm No change of F340 and emission kmax F340 decrease complete, peak shifts from 347 to 356 nm Minor change, 25% of total drop of h222...

Ngày tải lên: 08/03/2014, 23:20

... values of m and Dm The curve fittings were performed using MICROSOFT ORIGIN software Calculation of the rate constants from the kinetics of refolding HSA was found to follow two phases of refolding, ... The rate constant of the burst phase of the refolding step could not Fig The effect of GdnHCl on the far-UV CD spectra of HSA (A) CD spectra of HSA in the absence (s) and presence of 1.5 M (d), 2.5 ... in the presence of different concentrations of GdnHCl in the absence of HSA) from the original spectra 615 nm of the HSA–Nile red complex along with the changes in the wavelength of emission maximum...

Ngày tải lên: 16/03/2014, 16:20

... characterization of Gas1p Fig Analysis of culture supernatants from Pichia pastoris-transformed cells (A) Coomassie Blue staining of 100 lL of culture supernatant at 24 and 48 h from the shift (time zero) of ... catalytic domain of members of Family GH72 No data are available, to date, on the role of these residues Here we have shown that C74 of the catalytic domain is crucial for the proper folding of Gas1p ... fragments of GAS1 were amplified using two sets of primers For sGas1E161Q-H, a pairing of the forward primer XHup and reverse primer RMGLN161 (5¢-TGTTAGTAACTTGATTACCGGCGAAG-3¢), and a pairing of the...

Ngày tải lên: 16/03/2014, 18:20

... Closed-loop, multiobjective optimisation of analytical instrumentation: gas-chromatography- time -of- flight mass spectrometry of the metabolomes of human serum and of yeast fermentations Anal Chem 2005; ... end of the growing structure, where one of three different cap complexes is attached to help efficient selfassembly of particular proteins that need to be assembled at each specific stage of the ... Institute of Molecular Pathology (IMP), The Vienna Biocenter, Vienna, Austria E-mail: jenuwein@imp.univie.ac.at The last years were highlighted by the landmark description of the genomes of many...

Ngày tải lên: 16/03/2014, 22:20

... V of 23S rRNA These results, together with the characteristics of the two distinct conformational states, suggest putative cofolding of UDE and RNA during translation Physiological relevance of ... potential existence of two separate conformational states of the protein, one of these containing a signicant amount of associated nucleic acid To address the potential signicance of these conformational ... binding ability of RNA and DNA oligonucleotides with regard to both RNAUDE and UDE RNA binding signicantly affects thermal unfolding of UDE We followed the thermal unfolding of gel-ltrated UDE...

Ngày tải lên: 22/03/2014, 16:21

... effects of D2O on the dynamics of protein folding.[36] Knowledge of the influence of D2O on the conformational dynamics of a protein may be important both at a basic level, to identify the nature of ... number of cases,[25, 27] the stabilizing effect of D2O has been attributed to the enhancement of hydrophobic interactions However, the influence of D2O on the thermodynamic stability of proteins ... stabilizing effect of D2O on the thermal denaturation of several proteins, as induced by guanidinium chloride and urea[17, 24, 25] and from the promotion of aggregated states of oligomeric proteins.[26–28]...

Ngày tải lên: 22/03/2014, 18:20

... if agreed with the purchaser 8.12.5 Workmanship of ends The inside and outside edges of the ends of all product shall be free of burrs The threads of martensitic chromium alloys have shown a tendency ... before application of paints and related products —— Visual assessment of surface cleanliness —— Part 1: Rust grades and preparation grades of uncoated steel substrates and of steel substrates ... of longitudinal imperfections ISO/TR 9769, Steel and iron —— Review of available methods of analysis ISO/TR 10400, Petroleum and natural gas industries —— Equations and calculations for the properties...

Ngày tải lên: 23/03/2014, 00:20

... representation of ThermoFAD In this case, the increase in fluorescence is generated by exposure of the flavin cofactor to the solvent upon protein unfolding (C) Overview of fluorescence properties of flavins ... Evaluation of in vitro reconstitution of a protein complex using ThermoFAD C Fig (A) Evaluation of FMO stability using ThermoFAD against various buffers at various pH values (B) ThermoFAD comparison of ... of the bound flavin cofactor using published extinction coefficients Evaluation of the reliability of Thermo FAD versus Thermofluorâ for various flavoproteins To compare the results of the ThermoFAD...

Ngày tải lên: 23/03/2014, 04:21

... offering another route for therapeutic intervention [48,49] A clear understanding of the mechanism of the association of these cofactors with amyloid fibrils may expose further possibilities of ... lead to the accumulation of large quantities of partially folded proteins and the saturation of the capacity of the quality control machinery, exacerbating the formation of intracellular aggregates ... reducing the number of global unfolding events and decreasing the probability of subglobal unfolding and the consequent formation of partially unfolded states While the properties of the native proteins...

Ngày tải lên: 23/03/2014, 11:20