therapeutic applications of induced pluripotent stem cells in parkinson s disease

... homeodomain protein that is expressed from the Pou5f1 gene, is highly expressed in the inner cell mass (ICM) of the mouse embryos as well as in ESCs Besides its expression in ESCs, Oct4 is also highly ... generation of iPSCs from various species as well as from somatic cells belonging to different lineages52 13 Figure Reprogramming of somatic cells with transcription factors Somatic cells such as skin ... also be derived from germline stem cells harvested from neonate mouse testes43 as well as adult testes44 Interestingly, parthenogenesis of an unfertilised egg is also another means in which pluripotent...

Ngày tải lên: 09/09/2015, 18:56

... symptoms as distinct and different diseases, and others have given its name to diseases differing essentially from it" [1] For instance, dementia with Lewy bodies and Parkinson' s disease are ... impairment in Parkinson' s disease J Neurol Neurosurg Psychiatry 1988, 51:1498-1502 Hietanen M, Teräväinen H: The effect of age of disease onset on neuropsychological performance in Parkinson' s disease ... errors in separating idiopathic Parkinson' s disease from other causes of the akinetic-rigid syndrome, or errors in differentiating dementia from confusional states, depression and even ageing,...

Ngày tải lên: 08/08/2014, 21:20

... underscoring the need for using cohorts of cell lines and stringent statistical analyses to draw systematic comparisons between hESCs, healthy donor-derived iPSCs, and disease- specific iPSCs In support ... this article as: Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine ... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein...

Ngày tải lên: 11/08/2014, 12:21

... transposition system, in which a transiently expressed transposase catalyzes the excision of transgenes flanked by inverted terminal repeats [8,9] The PB system is effective in mouse and human cells, ... hinder widespread applications of iPSCs for various applications In addition, in the case of the recombinase-based approach, residual vector sequences are left behind, increasing the risk of insertional ... protocols, dopaminergic neurons were derived regardless of the age of the donor, thus highlighting the feasibility of using iPSC-derived cells in transplantation therapy for Parkinson s disease in...

Ngày tải lên: 14/08/2014, 21:20

... Immunocytochemical staining of SSEA1 The green fluorescence shows the GFP expression in A There was no positive staining of SSEA1 x that was detected in the red channel as shown in B DAPI was also used to stain ... Immunocytochemical staining of Sox2 The green fluorescence shows the GFP expression in A The positive staining of Sox2 is shown in red fluorescence in B DAPI was also used to stain the cell nucleus in C The ... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1...

Ngày tải lên: 30/09/2015, 10:13

... Suspension Culture of hPSCs 4.3.1 Creating a hPSC Suspension Culture 4.3.2 Splitting hPSCs in Suspension 4.3.3 Freezing hPSCs in Suspension 4.3.4 Thawing hPSCs in Suspension ... Thomson and his colleagues in 1998, considerable effort has been invested to improve methods of isolating new lines, in defined conditions, and to increase success rates This chapter discusses the ... peroxidase ICM ICR IHC IF IRS iPSCs ISCN IVF Inner cell mass Imprinting control region mice Immunohistochemistry Immunofluorescence Inner root sheath Induced pluripotent stem cells International system...

Ngày tải lên: 22/03/2014, 09:20

... was subjected to HPLC assay Figure HPLC analysis of the synthesis and release of DA by RPE cells HPLC analysis of the synthesis and release of DA by RPE cells. (A) HPLC analysis of standard of ... depolarization -induced DA release is not detected in the cells, indicating that the DA release machinery as seen in most excitable cells is not present in the RPE cells It 's possible that RPE cells may ... RPE-CM1protect SH-SY5Y cells from injury in the presence of neurotoxins RPE-CM protect SH-SY5Y cells from injury in the presence of neurotoxins (A) SH-SY5Y cells cultured without rotenone (Control), cells...

Ngày tải lên: 18/06/2014, 15:20

... Huntington s and Parkinson s diseases [17] Neurogenesis is increased in the hippocampus of Int J Med Sci 2008, brains of patients with AD, as revealed after autopsies by an increase in the expression ... neurogenesis in neurological diseases and disorders Adult neurogenesis is modulated in the brains of patients and in animal models of neurological diseases and disorders, like AD, depression, epilepsy, ... adult brain, neurogenesis is increased in the SVZ [54], as observed in brains of HD patients [41] These data provide evidences that adult neurogenesis is increased in the SVZ of brains with HD...

Ngày tải lên: 08/08/2014, 17:20

... paradigms was, however, significantly rescued when neural stem cells (NSCs) were transplanted into hippocampi Interestingly, these transplants also caused significant increases in synaptic density in ... and in the isolation of specific cell types of interest Within the past few years, iPSCs have been used to create in vitro models of other neurologic diseases, including PD, which often shares ... overlapping pathologies with AD Investigations into these diseases have shown that iPSC models are especially suited to the study of live cell and early aspects of disease pathogenesis For AD,...

Ngày tải lên: 11/08/2014, 12:21

... of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol (2):268-277 105 Tsai MS, Hwang SM, Chen KD, et al 2007;Functional network analysis of the transcriptomes ... W 1985;Lineage of osteogenic cells and their relationship to the stromal system In ed Bone and MineralAmsterdam Elsevier pp :1-25 122 Owen M 1988;Marrow stromal stem cells J Cell Sci Suppl :63-76 ... expression in 3T3adipocytes Cell (3 Pt 2):657-666 basal lamina molecules expression in human subcutaneous white adipose tissue Eur J Histochem (3):183-188 137 Kapuscinski J 1995;DAPI: a DNAspecific...

Ngày tải lên: 11/09/2015, 09:16

... immune system has also been identified in the disease [1,6] Furthermore, involvement of TLRs has been shown in other systemic autoimmune diseases [16] In the present study, expression levels of TLR2 ... by using Scion image analysis and image processing software (NIH Image Engineering, Bethesda, MD, USA) Statistical analysis Mann-Whitney U-test, Kruskal-Wallis test with post-hoc Scheffe 's test, ... HO-1 expression by using specific small interfering RNA resulted in upregulation of synthesis of proinflammatory cytokines, including interleukin-6, interleukin-8 and TNF, which have been shown to...

Ngày tải lên: 09/08/2014, 10:22

... pathogenesis Therapeutic studies in AD models Access to good animal models is crucial to success in developing disease- modifying therapeutics However, AD neuropathology is incomplete in the Ab-expressing ... cross-breeding experiments is strengthened if expression and deletion of the gene (s) results in the opposite outcome, or if the results are substantiated by in vitro studies or analyses of clinical samples ... expression patterns of APP and steady-state Ab levels in the central nervous system and in peripheral tissue; consequently, interpretations from transgenic models regarding Ab dynamics should...

Ngày tải lên: 16/02/2014, 09:20

... penetrance of LRRK2-associated Parkinson s disease: a case-control study Lancet Neurol 7, 583–590 Sen S & West AB (2009) The therapeutic potential of LRRK2 and alpha-synuclein in Parkinson s disease ... verification of endogenous expression is difficult to assess in most cell lines as a result of the presumed low levels of protein and a lack of potent antibodies [36] On a subcellular level in the brain, ... West containing interleukin receptor-associated kinase family Many of these kinases have clear roles in the MAPK pathway, and MLK proteins serve as critical mitogen-activated protein kinase kinase...

Ngày tải lên: 23/03/2014, 04:20

... Fahn S: Motor blocks in Parkinson s disease Neurology 1992, 42(2):333-339 Hong M, Earhart GM: Rotating treadmill training reduces freezing in Parkinson disease: preliminary observations Parkinsonism ... freezing of gait in parkinsonism, proposed working definition, and setting the stage Mov Disord 2008, 23(2) :S4 23-425 Backer JH: The symptom experience of patients with Parkinson s disease J Neurosci ... severity to assess changes after an intervention using videotaped sessions of five 10-meter walks including turns A previous study reported the directionally restricted effects of gait training...

Ngày tải lên: 19/06/2014, 08:20

... an increased fall risk in older adults in general, as well as in patients with PD [9-13], suggesting that this aspect of gait may have clinical utility as an aid in fall risk assessment More specifically, ... Glatt S, Vetere-Overfield B, Hassanein R: Falls and Parkinson' s disease Clin Neuropharmacol 1989, 12:98-105 Bloem BR, Hausdorff JM, Visser JE, Giladi N: Falls and freezing of gait in Parkinson' s disease: ... the forces underneath the foot as a function of time The system consists of a pair of shoes and a recording unit Each shoe contains load sensors that cover the surface of the sole and measure the...

Ngày tải lên: 19/06/2014, 10:20

... characteristics in the Parkinson' s disease and control groups during 6-minute walking with and without stepsynchronized vibration stimulation Locomotion Parameters Parkinson' s Disease Group Control ... TL: The sensitivity of the sole of the foot in patients with Morbus Parkinson Neurosci Lett 2003, 346:173-176 Zia S, Cody F, O'Boyle D: Joint position sense is impaired by Parkinson' s disease Ann ... the soles Vibration intensity is similar to that of portable devices such as cell phones and beepers, operating in the vibration mode VD was mounted on shoe insoles inserted into the shoes The...

Ngày tải lên: 19/06/2014, 10:20

... Progression of Parkinson s Disease Figure stimuli Schematization of responsiveness of PD subjects to visual Schematization of responsiveness of PD subjects to visual stimuli The diagram represents schematically ... controls in response to an attentional stimulus (increasing stride length, decreasing cadence), would suggest that, in advanced stages of disease, as a consequence of progression of motor symptoms, ... number of subjects included in the AS condition (data missing in four out of 15 PD subjects for AS), two distinct statistical comparisons have been performed: 1) baseline vs BOF vs FOF, 2) baseline...

Ngày tải lên: 19/06/2014, 10:20

... domains for discussion in the focus groups of PD patients with off-time: feelings of anxiety, mood swings, loss of interest, fatigue and autonomic or gastrointestinal symptoms (such as excessive ... Component Summary; PD: Parkinson s disease; PDQ-8: Parkinson s Disease Questionnaire-8; RMSEA: root mean square error of approximation; SCOPA-DC: Scales for Outcomes of Parkinson s disease Diary ... breaks, and excessive sweating as a result of very simple tasks such as walking while shopping were also frequently mentioned by patients There was strong endorsement of these symptoms appearing in...

Ngày tải lên: 20/06/2014, 15:20

... Despite these efforts to reduce radiation -induced late toxicity, of which heart disease is one aspect, oncologists and cardiologist are still seeing survivors of Hodgkin 's disease treated in ... As part of an ongoing interdisciplinary study between cardiologists, radiologists and radiation oncologists, Hodgkin 's disease survivors will be invited for extensive cardiologic tests including ... [7] In a subsequent series of trials, involved-field doses of 20 Gy and 30 Gy were compared in low-risk and intermediate-risk groups and early analyses in intermediate-risk patients suggest equivalence...

Ngày tải lên: 09/08/2014, 10:20

... versions of the PDQ-8 used in the present study were validated previously.64, 80, 81 Statistical analysis Responsiveness of PDQ-8SI scores was assessed using responsiveness indices including ... national institute of neurological disorders and stroke PD - Parkinson s disease PDQ-8 - the 8-item Parkinson s disease questionnaire PDQ-8SI - the 8-item Parkinson s disease questionnaire summary index ... Amongst Asian Patients with Parkinson s Disease 13 Chapter Factors affecting HRQoL amongst Asian patients with PD Zhao, Yingjiao INTRODUTION Parkinson s disease (PD) is a chronic, progressive...

Ngày tải lên: 11/09/2015, 10:05