the workhorse lipids for crystallizing membrane proteins in mesophases

... the proteins of the outer membrane, we set out to determine what proportion was integral and to identify the major protein species To determine whether the major outer membrane proteins were integral ... least 19 integral membrane proteins partitioned into the detergent phase: Om45 and Om14 clearly behaved as integral membrane proteins with a-helical transmembrane segments, partitioning into the detergent ... N-terminal FAD-binding domain facing the intermembrane space This topology would enable Gut2 to transfer of electrons to Nde1 in the intermembrane space [32] Alo1 catalyses the final step in d-erythroascorbic...

Ngày tải lên: 19/02/2014, 07:20

Ngày tải lên: 07/11/2012, 14:21

... V.2.Recommendation…………………………………………………………………… ……30 V.2.1.Selecting and evaluating the texts……………………………………………… ……30 V.2.2 Suggested techniques for integrating short stories and poems in the teaching of reading comprehension skills………………………………………………………… ... links between reading skills and literary skills…………… ……………… 13 II.3.2 Efferent reading and aesthetic reading……………………………….………………14 Chapter III: Methodology……………………………………………………………………15 III.1 .The ... texts……………………………….………………19 IV.2 .The effects of short story and poem in teaching reading comprehension….………22 Chapter V Conclusion and Recommendation………………………………… ………30 V.1.Introduction…………………………………………………………………………...

Ngày tải lên: 07/11/2012, 14:21

... happens during the actual reading The purpose of the former is using the text to gain information while that of the later is exploring the language usage through the relevance to the experience ... to them As a result, they get involve in the text and interact with the text by sharing their feelings or experience Furthermore, if a reader in efferent reading is supposed to carry the information ... readers in aesthetic reading the enjoyment is attained by interacting with the text, they often relate their world of experience to the text After reading they might be asked whether something similar...

Ngày tải lên: 07/11/2012, 14:21

... remember them longer and understand their usage □ Others……………………………………………………………… e Interpreting characters in the short story or figurative meanings in the poem □ I think understanding the content ... thoroughly □ Others……………………………………………………………… d Skimming for idioms and expressions in the full sentences □ It is useless, we can look them up in a dictionary □ It is useful, we can learn them in the context, ... Questionnaires on the effects of short story and poem in teaching reading comprehension Which of the followings you find most difficult when reading short stories and poems: a unclear theme b vocabulary...

Ngày tải lên: 07/11/2012, 14:21

... conclusion The focus of the study  Pointing out the values of literature included short stories and poem in language teaching and finding the way to integrate short stories and poems into the reading ... learning reading Method of collecting data:  At the end of term, weeks before the final exam Results and Findings  Results:  Pointed out that integrating short stories and poems in reading ... teaching reading comprehension Introduce techniques to integrate literary texts in the reading class Scope:   The importance of learning English in the industrial world The situation of learning and...

Ngày tải lên: 07/11/2012, 14:21

... nonspecific metal binding Specificity of Zn(II) binding by the purified Zn(II)-binding proteins The specificity of Zn(II) binding was also examined for the purified Zn(II)-binding proteins The binding of radioactive ... plasmids for zinc-binding proteins The genes coding for zinc-binding proteins were amplified by PCR using the whole genome DNA of E coli W3110 (A) as template The forward primers used included the ... Protein sequence analysis for the isolated Zn(II)-binding proteins In order to identify the nature of proteins showing Zn(II)binding activity, we isolated the major Zn(II)-binding proteins from...

Ngày tải lên: 22/02/2014, 04:20

... affects the slope of the transition region In the original equation, this parameter is equal to for a univalent base (and -1 for a monoprotic acid) The pKa values listed in Table for the bivalent lipids ... after the addition of DOPE alone or in combination with cholesterol Increasing the + ) charge ratio beyond : resulted in decreased transfection activity for the most active derivatives in all formulations ... transfer, namely the polar headgroup, the nonpolar tail (either a cholesterol moiety or a pair of aliphatic hydrocarbon chains), and the linker tethering both regions together Since the advent of...

Ngày tải lên: 23/03/2014, 07:20

... distance by varying the linker can be used in vivo to examine the effect of host proteins on probe geometry Regardless of the coupling of the uorophores to the linker, all of the host proteins that ... stFRET proteins were constructed with Streptag II linked to the C-terminaus for single-tagged stFRET and to the C-terminus and N-terminus for doubletagged stFRET These proteins were allowed to bind ... distributes, we incorporated it into host proteins, including nonerythrocyte spectrin, lamin A and a-actinin (Fig 10A,C,E) in 3T3 cells, and COL-19 in C elegans (Fig 10G) The distribution of the probe...

Ngày tải lên: 30/03/2014, 04:20

... modulating these mitochondrial functions [27] Further, we found that AR-sensitive proteins had higher degree in the regulatory interaction network reinforcing the importance of disrupting aromatic interactions ... enrichment in RNA metabolism for both AR and ABsensitive proteins and in DNA binding for AR and hydrophilicity-sensitive proteins In addition, charge-sensitive proteins showed an enrichment in transferase ... Finally, the discriminator examines if the label is meaningful for any of the protein targets If aromaticity were significant in determining the disruptiveness of a particular drug to that protein,...

Ngày tải lên: 09/08/2014, 22:24

... platform for the recruitment of Nanos (Nos) and Brain Tumor (Brat) proteins to repress the translation of hunchback mRNA in the posterior region of the embryo The RNA binding domain of Puf proteins ... observed for Drosophila Pumilio PUM-HD binding to the NRE1 (Kd ~0.5 nM; [34]), but within the range observed for other Puf protein interactions with their cognate RNAs [21,28] The binding affinity ... exon-junction complex of proteins, RNA-binding proteins, and other proteins localize to nucleoli in plant cells supports a role for the nucleolus in mRNA processing, silencing, surveillance and...

Ngày tải lên: 12/08/2014, 03:21

... and interpretation of data JAL, JN, FA, and MMP were involved in drafting the manuscript and revising it critically for important intellectual content All authors have given final approval of the ... Competing interests The authors declare that they have no competing interests References Bellomo R, Ronco C, Kellum JA, Mehta, RL, Palevsky P and the ADQI workgroup: Acute renal failure - definition, ... kidney injury, defined by means of the RIFLE criteria, is common among critically ill HIV-infected patients, and RIFLE seems a helpful tool in the prognostic stratification of ICU HIV-infected...

Ngày tải lên: 13/08/2014, 03:20

... SPRINT is limited to U/hour to minimise saturation and the administration of ineffective insulin [23,29,30,43] Insulin is given predominantly in bolus form for safety, avoiding infusions being ... challenges the practice of using insulin-only protocols In the presence of significant insulin resistance, insulin effect saturates at high concentrations of insulin [23,29,30], limiting the achievable ... SPRINT, Specialised Relative Insulin Nutrition Tables Figure shows the average nutrition intake and insulin administration rate for the first days on the SPRINT protocol The average nutrition intake...

Ngày tải lên: 13/08/2014, 10:20

... considered In 10 patients proving too instable for transport, our mobile team implanted the portable venovenous ECMO in the referring hospital Patient characteristics before inclusion are specified in ... possible in the CESAR trial and five patients in the ECMO group died before transport or in transit With the miniaturized device of the present study, interhospital transport has been carried out in ... hemorrhage, the latter 32 days after weaning Discussion The current study presents for the first time the use of a new miniaturized system for extracorporeal membrane oxygenation in a large adult...

Ngày tải lên: 13/08/2014, 20:21

Ngày tải lên: 26/03/2015, 10:25

... related information from the point of origin to the point consumption The aim is to maximize the performance of the entire distribution system Order processing The logistic process starts with the ... factor in the high growth rate of nominal market BMI predicted overall inflation rate increased sharply at 11.5 % in 2011 before declining to 5% per year in the second half of the 10 -year forecast ... 20 % in 2004 Table The index of medications business in the Asia – Paciffic area in the quarter I - 2011 AWARD The RISKS The The The Percentage award award Japan 70 70 Australia 60 Korea of in...

Ngày tải lên: 26/03/2015, 10:58

... of the genes encoding Cg-BMPR1 and Cg-TGFbsfR2 is shown in Fig 2C The serine ⁄ threonine kinase domain in both proteins is encoded by two exons equivalent to kinase subdomains X and XI [22] In ... of the TGFb superfamily type I receptors are in bold and underlined, and the cysteine knot is boxed Also boxed are the transmembrane domain, the ATP binding site, the L45 loop and the serine ... clarify the binding properties of each of these domains we generated synthetic mRNA encoding only the C1 or C2 domains Expression of only the C1 domain resulted in the dorsalization of embryos in...

Ngày tải lên: 07/03/2014, 21:20

... the permeability of the IM of E coli, · 106 cells were mixed with lm SYTOX Green in NaCl ⁄ Pi for in the dark After addition of peptide, the increase in fluorescence, owing to the binding of the ... different initial surface pressures (p0), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the ... 6707 the peptide-induced membrane breakages, and substantial roughening of their surface The peptide might bind to the membrane surface in a carpet-like arrangement, inserting into the polar phospholipid...

Ngày tải lên: 16/03/2014, 00:20

... Some membrane proteins incorporate a hydrophobic pore-lining region that does not cross the membrane, but instead forms a beta hairpin structure that dips into the membrane then re-emerges on the ... innexins contain four transmembrane domains, and combine to form dodecamers [14,16–19] In contrast to ligandgated channels, the four transmembrane domains of connexin and innexin are organized into ... containing six membrane- associated domains (Fig 1B) [14] In voltagegated Na+ and Ca2+ channels the four subunits are linked together as a single protein with a series of internal repeats In the...

Ngày tải lên: 17/03/2014, 23:20

... both hydrophobic domains (one in the signal peptide, the other in the mature protein) insert into the membrane and form a transmembrane loop intermediate [20] As a result, the hydrophilic, negatively ... that they may insert spontaneously into the thylakoid membrane The initial stages of this spontaneous insertion mechanism involve binding of the intermediate-size protein to the membrane, after ... domain is translocated across the membrane to the lumen where further processing by a thylakoid processing peptidase (TPP) removes the remaining presequence leaving the mature protein inserted in...

Ngày tải lên: 18/03/2014, 01:20