... YidC and Sec play a role in the translocation of the C-terminal loop with the T7-epitope tag. Taken together, the data presented here show that KdpD inserts unassisted from the Sec translocase and ... restriction site between the first and second and between the third and fourth helices by site- directed mutagenesis. The epitope tags were introduced into the opened MunI sites of the respective plasmids ... to the medium. Wild-type KdpD, KdpD containing the HA- and T7- epitope tags, KdpD-N containing the N-terminal fragment with the HA-epitope and KdpD-C containing the C-terminal fragment with the...
Ngày tải lên: 20/02/2014, 11:20
Ngày tải lên: 22/07/2014, 04:20
Tài liệu Data and Information on Women’s Health in the European Union docx
... Finland, France, Germany, Iceland, Italy, the Netherlands, Norway, Poland, the UK, Slovenia, Spain, Sweden, and Switzerland) increased from 11% in the period 1988-1990 to 13% in 1997-1999. The ... indicator is meant to complement life expectancy data and provides information on the quality of years lived rather than the quantity. HLYs also provide information on the structural and nancial ... Denmark, Finland, France, Germany, Iceland, Italy, the Netherlands, Norway, Poland, the UK, Slovenia, Spain, Sweden, and Switzerland) increased from 48% to 54% in both sexes in the period 1988-1999...
Ngày tải lên: 12/02/2014, 23:20
Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... well as the former simulation as they do to the experimental data. The large subunit NrfA, contains the high-spin heme, and four low-spin c-hemes with g max ẳ 3.6, 3.50, 3.2 and 2.96, while the NrfH ... containing the double of NrfA subunit in respect to the NrfH subunit. Nevertheless, inside these huge aggregates there are species of the type a 2 b 2. The present findings are in agreement with the ... buffer. According to the column calibration, the molecular masses of the two observed peaks (Table 1) are in good agreement to the NrfA and NrfH molecular masses, as further proved by the respective SDS/PAGE...
Ngày tải lên: 21/02/2014, 00:20
The Financial Crisis and Information Gaps: Progress Report Action Plans and Timetables pptx
... only the timeliness of the publications and the availability of the data on the BIS website, but also the coverage of the data to additional G-20 economies. Both the BIS and IMF continue to work ... priority. The experience of the ECB and Eurostat within Europe and the OECD should be drawn upon. In the medium term, including more sectoral balance sheet data in the data categories of the Special ... category, and sector. Agreements have been reached by the BIS with the reporting central banks to report these new datasets. The degree of granularity of the data released by the BIS will then be...
Ngày tải lên: 06/03/2014, 19:20
The Financial Crisis and Information Gaps: Implementation Progress Report potx
... 2011. The work of the BIS on banks can be further enhanced if the CGFS approves the enhancements to the IBS data (see recommendations 10 and 11). 5. The CGFS and the BIS to undertake further ... Most of the coordination work has been undertaken through the IAG, with the existing relevant bodies, such as the BOPCOM, the FSB, and the CGFS taking the decisions for the datasets under their ... of the IMF Data Standards Initiatives, scheduled for the first half of 2012, includes the possibility of strengthening the Data Standards, including integrated sectoral balance-sheet information, ...
Ngày tải lên: 06/03/2014, 19:20
The common management information services and protocols for the internet (CMOT and CMIP)
Ngày tải lên: 16/07/2014, 08:55
Báo cáo y học: "Closure of the NCBI SRA and implications for the long-term future of genomics data storage" doc
Ngày tải lên: 09/08/2014, 22:23
Tài liệu THE HISTORY OF THE RISE, PROGRESS, AND ACCOMPLISHMENT OF THE ABOLITION OF THE SLAVE-TRADE pdf
... liberty, and make them slaves and sell them, are the worst of robbers, and ought to be considered as the common enemies of mankind; and that they who buy them, and use them as mere beasts for their ... knowing all the circumstances connected with them, found their own zeal increased, and their own courage and perseverance strengthened; and have they not, by the communication of them to others, ... continent of America and the islands adjacent, and were to join the crimes and sufferings in one to those in the other, by the crimes and sufferings which took place in the track of the vessels successively...
Ngày tải lên: 15/02/2014, 09:20
Tài liệu Good Governance and Aid Effectiveness: The World Bank and Conditionality docx
... politics. The Bank’s understanding of good governance continues to reflect a concern over the effectiveness of the state rather than the equity of the economic system and the legitimacy of the power ... strengthening governance systems and enhancing governing institutions. The modernization of the state, the fight against corruption, the reform of judiciary, the strengthening of legislatures and the ... these research findings, the use of conditionality has expanded in scope and depth in the course of the 1990s. As the IMF itself recognizes, the share of programs with structural conditions and...
Ngày tải lên: 16/02/2014, 12:20
Tài liệu Safety and security on the Internet Challenges and advances in Member States docx
... quality of the information they locate online becomes paramount. To this end, the Health On the Net Foundation (HON) aims to “help unify and standardize the quality of medical and health information ... France (48). In the beginning, HON’s strategy and vision in improving the quality of medical and health information on the web was not well-known. In 2004, the European Commission and the European ... practically all of the samples they purchased online during their study. Many of the drugs had no or minimal information on their labels regarding the proper usage of the product, and some were...
Ngày tải lên: 18/02/2014, 00:20
Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... tyrosinase (data not shown). These data confirm the great preference of oxy-tyrosinase for monophen- ols, so that the DO activity is not competing with TH during the course of the reaction, and the chemically generated ... bp between the two copper- binding sites from this gene was amplified using the appropriate forward and reverse primers. Then the product was cloned in the pFSVK plasmid using the NcoI and SacI ... at pH 7. These preferred activities of the two PPOs of R. solanacearum are opposite to the names assigned to them when the genome of this bac- terium was sequenced and the function of these con- ceptual...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
... the determination of the N-terminal sequences, C. Hunzinger for the MALDI-TOF analyses, C. Bache and J. Hermanns for their help with the cloning experiments, and J. R. Harris for correcting the ... untranslated regions and the entire 3Â untranslated regions. The standard polyadenylation signals (AATAAA) and the poly(A)-tails of different lengths are present in each clone. The open reading frames of the ... evolved before the separation of the Chilo- poda and Diplopoda more than 420 million years ago. In contrast to the situation in the Crustacea and Chelicerata, the substitution rates among the myriapod...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
... at 5m M EDTA and plotted as a function of the free Mn 2+ concentration (Fig. 2B). The data show that synthesis and exchange with or without CMP behave in the same way according to the concentration ... where the authors have identified the reverse products and shown that the CMP activation of the reverse of synthesis is by a different mechanism from base exchange [20]. Further analysis of the ... plants, for 20 years, the lack of cloned sequences had prevented the investigations necessary to determine whether the situation is the same as in yeast; however, the cloning and expression of a...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu seed policy and programmes in the near east and north africa pdf
Ngày tải lên: 23/02/2014, 15:20
Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex could be one of the fastest ... the membrane. The position and function of the long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15]. According to one hypothe- sis, the hydrophilic Tic110-Ct faces the stroma, ... translocation across the envelope is surely a bottleneck in the path of trans- ported proteins from the cytosol to their final destina- tion in the chloroplast. The Tic and Toc translocons are therefore...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... experimental and calculated values of these parameters. There are three possible reasons for the differences between the crystal and the solution structures of the M domain. First, the orienta- tion of the ... obtained. The overall fold and the structure of the b-strand core of the protein in solution are similar to those found in the crystal. However, the orientation of the functionally critical GGQ loop and neighboring ... times, particularly to the correlation time of the slow motion s s . The range of the dataset on the NOE axis is very sensitive to the value of order parameters; the width of data distribution along the R 2 ⁄...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot
... similarities in their tertiary structures, related to their ligand-binding specificity and affinity [10]. In addition to the similar three- dimensional structure and ligand-binding specificity and affinity ... zebrafish fabp4 and fabp3 and other fish and mammalian FABP genes was performed using clustalx [43]. The Antarctic fish H6-FABP and H8- FABP sequences were included in this analysis, and the putative ... hybridization signal in the spinal cord and the appearance of transcripts at the level of myosepta in muscle pioneers (Fig. 6A). At 48 hpf, in addition to their continued presence in the lens and dorsal diencephalon,...
Ngày tải lên: 07/03/2014, 10:20