the structure and process of creating business messages

... model of the role of point of view in problem solving. SUMMARY We have reported here a three pronged approach to the study of problem solving action and report: I) the collected of data ... solving and talk about problem solving, 2) development of a process model of these behaviors, and 3) use of coding techniques to extract traces of "critical phenomena" from the transcripts ... relltld ~O the ~r~romnoe oF 5he problem tooK, lad o~here tt~c are oZoeely related to the report of the task aoClona. ~n the psr~lo,,~ar problem demean of the H~aeionsr£ee and CannLbae8 pusxle,...

Ngày tải lên: 21/02/2014, 20:20

... W,is simply the sum ofthe body mass of all individuals. For organisms ofsimilar size, it can be estimated by taking the product of the population, N,andthebodymass, M. Similarly, the store of each ... obvious. We asked the author(s) of each paper to examine the importance and role of body size in the systems in which they work. Essentially the book builds from the level of the individual and a consideration ... level to the flux, storage and turnover of these elements at the level of ecosystems. Our second example concerns the role of metabolism in trophic relationships, including the structure and dynamics...

Ngày tải lên: 17/02/2014, 19:20

... resemble teardrops [32]. Further examination of the structure and sequence of sbwAFP and TmAFP reveal other similarities (Fig. 8 C). The panel shows the similarity of the TXT face again, a nd also ... determine the handedness of the proteins, or may prevent the unfolding of the protein at cold temper- atures. The b-helix as an AFP structural motif? The sbwAFP and TmAFP structures represent the first AFPs ... of X-ray structure with 5 °C NMR structure using the main chain of residues Ser12 fiThr70 in t he structure a lignment. (B) O verlap of the X-ray structure with the 30 °C NMR structure using the main...

Ngày tải lên: 19/02/2014, 16:20

... orm of b strand [18]. The positive bands at » 1675 cm )1 in the amide I r egion of the ROA spectra of b-andj-casein, which originate mainly in the peptide C  O stretch, are characteristic of ... (although there is a hint) in the ROA spectra of b-andj-casein may be evidence that, as suggested previously [3], the b -structure identi®ed above mainly t akes the f orm of unassociated b strands rather ... positive ROA at » 1320 cm )1 to PPII structure relies mainly on the evidence outlined above. In view of the c lose similarity of the ROA spectra of the casein, synuclein and tau proteins shown in t his...

Ngày tải lên: 21/02/2014, 15:20

Ngày tải lên: 08/03/2014, 23:20

Ngày tải lên: 15/03/2014, 23:20

Ngày tải lên: 16/03/2014, 16:20

Ngày tải lên: 23/03/2014, 07:20

Ngày tải lên: 22/06/2014, 00:20

Ngày tải lên: 12/12/2013, 00:15

... between the Leu residues from v-KIND and MAP2 contributes to the interaction between the two proteins. In conclusion, the present study has clarified the structural and functional importance of the ... and 482) and one Thr residue (amino acid 487) were well conserved in the KIND2 of v-KIND in all species analyzed, although not in the other KINDs. To investigate the possible involvement of these ... under- stood, the data obtained in the present study indicate that the 43 residues (amino acids 702–744 in mice) that reside in the middle region of the CD act as the v-KIND binding core. The v-KIND-binding...

Ngày tải lên: 14/02/2014, 19:20

... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra ... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spectra were used to calculate the fraction of Pyr-ONC (M23L), ... simplify the protocol and shorten the time needed to obtain a fully active ONC compared with other procedures described in the literature. The application of MALDI-TOF MS to the characterization of...

Ngày tải lên: 19/02/2014, 12:20

... basis for structure- activity studies. The high selectivity of the a-conotoxins, together with the possibility of obtaining detailed information on their three dimen- sional structures and the relative ... native and heterologously expressed nAChRs The examples presented above clearly demonstrate the usefulness of a-conotoxins in the determination of the structure and function of native nAChRs, and ... so far. The great variability of the conotoxins and their highly specific action on different ion channel subtypes derives from the structure of the peptides which have evolved conserved and hypervariable...

Ngày tải lên: 19/02/2014, 12:20

... Fragments of HSQC and HMBC spectra of compound 1. Labels illustrate assignment of the amide linkage between the amino group of Asp and the carboxyl group of GalA residue E. Scheme 1. The structures of ... min), dried and analyzed by GC-MS with the standards prepared from D -GalN and (R)- and (S)-2-BuOH. The absolute configuration of L -aspartic acid was deter- mined by chiral HPLC of the oligosaccharide ... allow u s to p ropose the structures of oligosaccharides 1 and 2, as presented in Scheme 1. The o ligosaccharides were further analyzed by CE-MS and by CE-MS/MS (Figs 5 and 6). The mass spectra obtained...

Ngày tải lên: 19/02/2014, 16:20

... cADPR analogues revealed new insights into the structure activity relationship. Substitution of either the northern ribose or both the northern and southern ribose resulted in much simpler molecules, which ... northern and southern riboses are pri- marily necessary as linkers between the base adenine (or hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands. These ether ... ether strands. These ether strands mimic the distance between the nucleo- base and the diphospho-bridge, but on the other hand likely are involved in polar interactions with the cADPR receptor protein....

Ngày tải lên: 20/02/2014, 01:20

... SDS/PAGE and in 2D PAGE (Figs 1 and 2; Table 1). The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... against the hemocyanin fraction show staining of the two 75 kDa bands in Western blot, but do not recognize any other proteins of the hemolymph. Although we expected that each of these bands contains two ... primers and the T3 vector primer. The sequences were obtained after the cloning of the PCR products into the pCR4-TOPO TA vector (Invitrogen). Protein structure modeling Homology models of the S....

Ngày tải lên: 20/02/2014, 11:20

... us. Neither of these parameters are within the range of our experimental observations, and therefore the predominant species in solution are the monomer and dimer. Additional evidence that the crystallographic ... arrestin and arrestin in the presence of phosphopeptide is given at the top of the (B) and (C), expressed as a percentage of the total signal from the detector. Ó FEBS 2002 Activation of visual ... provide the radius of gyration (R g ), defined as the root mean square distance of all atoms from their common centre of mass. The R g canbeusedtoevaluatethe overall shape of a scattering particle. The...

Ngày tải lên: 22/02/2014, 07:20

... those in the TG structure. The arrow indicates the Tyr27 residue. The mean and SD of the val- ues are 0.25 A ˚ and 0.15 A ˚ , respectively. (B) The backbone atoms of liganded and unliganded structures ... Displacement of the backbone atoms of Mglu and Bacillus glutaminase induced by ligand binding. Displacement val- ues for backbone atoms of the nonliganded and liganded structures are shown. The average and ... 4. Effect of L-glutamate and Tris on backbone atoms. Backbone atoms of the overall amino acid residues of the T and TG (T ⁄ TG), N and G (N ⁄ G), G and TG (G ⁄ TG), and N and T (N ⁄ T) structures...

Ngày tải lên: 06/03/2014, 09:22

... region of the 1 H, 15 N-HSQC spectrum of the C-terminal domain of human eRF1 illustrating the presence of two conformational states of the protein. Fig. S2. Plot of the number and distribution of the NOEs ... However, these structures do not show the coordinates of the atoms in Fig. 6. Superposition of the representative NMR open conformer of the C-domain (red and blue) on the crystal structure [20] of the complex ... 0.08 Percentage of residues in the most favorable region of the Ramachandran map 91.1 85.5 Percentage of residues in disallowed region of the Ramachandran map 00 Superimposition of the structures on the...

Ngày tải lên: 06/03/2014, 11:20

... of the structure of NADP + -bound FeR revealed that the nicotinamide moiety of NADP + faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182–Lys186, pass through the a2 vicinity of the other monomer. For the FeR dimer, the N-terminal loop ... present the crystal structure of DvMF flavoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures. Results Cloning and sequencing of the flavoredoxin...

Ngày tải lên: 07/03/2014, 02:20