the role of the ikaros gene family in lymphocyte development
The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
Ngày tải lên :
25/10/2013, 21:20
... for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins Mdm2 (as well as c-Abl) binds to the C-domain All pocket proteins ... cellular proteins Although the binding site for LXCXE motif containing proteins is present in the B subdomain, the crystal structure of the pRb A/B pocket bound to the LXCXEcontaining part of HPV-16 ... expression of an E2F variant containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing...
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Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
Ngày tải lên :
22/02/2014, 07:20
... function of the hormonal system We first cloned the genes of the CYP11B family of the guinea pig The 11bhydroxylase of the guinea pig showed higher substrate specificity than the aldosterone synthase In ... 6) for the remaining introns Finally, the 3¢ UTRs of the guinea pig CYP11B genes show only 42% similarity whereas the 3¢UTR of the guinea pig CYP11B2 gene shares up to 72% identity with the corresponding ... position 366 while all other proteins of the CYP11B family including CYP11B2 of the guinea pig have a basic residue (arginine or lysine, respectively) at the corresponding position These two basic residues...
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báo cáo khoa học: " Unexpected complexity of the Aquaporin gene family in the moss Physcomitrella patens" pdf
Ngày tải lên :
12/08/2014, 05:20
... divided into five subfamilies; the plasma membrane intrinsic proteins (PIPs), the tonoplast intrinsic proteins (TIPs), the nodulin-26 like intrinsic proteins (NIPs), the small basic intrinsic proteins ... affecting the permeability of the pore since the orientation of the arginine of the ar/R filter is also stabilised by a hydrogen bond to the backbone of the C-loop (Fig 5) Interestingly all the ... situated in the plasma membrane (WoLF PSORT [51], data not shown) In the first NPA box of the XIPs, the alanine is replaced by a valine, leucine, isoleucine, serine or cysteine All of these replacements,...
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The Role of Small and Large Businesses in Economic Development doc
Ngày tải lên :
06/03/2014, 19:20
... at the beginning of the period, which favors a finding of higher growth among small firms, rather than at the end of the period Table A1 decomposes job growth from the second quarter of 2000 into ... and income generated directly by the firm—would certainly suggest this to be the case In reality, however, it is often the effects on other firms in the area the indirect effects—that carry the ... misleading to measure net employment changes as total employment in a size class at the end of the year less total employment in the size class at the beginning of the year The numbers presented in...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 5
Ngày tải lên :
16/09/2015, 17:13
... Chapter General conclusions Based on the findings presented in this thesis, the following general conclusions can be drawn: 1) At least seven distinct vitellogenin genes (vtgs) were found in the ... studies indicated that in addition to the female liver, vtg mRNAs were also present in several non-liver tissues, including the intestine, ovary, muscle and skin in female fish, and the intestine, ... skin and gill in E2 treated male fish In extra-hepatic tissues examined, the expressions of vtgs were localized mostly in adipocytes Interestingly, enlarged vascular termini composing mainly of...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 4
Ngày tải lên :
16/09/2015, 17:13
... 32P injected fish, the collective radioactivity in the remaining parts of fish (labeled as “others”) was obtained by measuring the radioactivities of portions of the remaining tissues and then ... reduction (when modifying 5-15% of the lysine or arginine residues) or loss (when modifying 34-50% of the lysine or arginine residues) of the capacity for the derived Vtg to bind with its receptor ... multiplying accordingly based on the proportion of the sampled tissues in the remaining parts of fish (by weight) The “radioactivity recovery rate” was defined as the total radioactivity of whole...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 3
Ngày tải lên :
16/09/2015, 17:13
... detected in the intestine, spleen and the surrounding tissues, indicating that mRNAs had been well preserved in these tissues Thus, the location of vtg-expressing cells in the intestine is limited ... hybridization signal in the intestine was not due to degradation of mRNAs, the integrity of mRNAs in the intestine was examined by hybridization with a β-actin antisense riboprobe As shown in Fig 3-11C, ... Expression of vtgs 3.1 Introduction The major sites for Vtg synthesis in egg-laying animals (oviparous) include the fat body (in insects), intestine (in nematodes and echinoderms) and liver (in vertebrates)...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 2
Ngày tải lên :
16/09/2015, 17:13
... (Danio rerio) vtg multigene family, including identification of individual vtg members, elucidation of the primary structures of Vtg proteins, mapping of vtg genes and inferring the evolutionary relationships ... parts of Vtg proteins are the PV domain and the homologous subdomains IV and V The exact role of the PV domain is unknown, but it has been proposed to be involved in receptor binding during receptor-mediated ... used in phylogenetic analysis by the parsimony method using the phylogenetic program PAUP v3.1 (Swofford, 1993) The input file was in NEXUS format following the PROTPARS example included in the...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 1
Ngày tải lên :
16/09/2015, 17:13
... amplification of the whole vtg cluster, leading to a three-fold increase in the total vtg copy number Although the length of the coding sequence is similar, the intron number and the average intron size of ... and the opportunity of introducing of fluorescent report genes, such as green fluorescent protein (GFP) gene in the fish The application of GFP transgenic zebrafish in developmental studies includes ... through the caudal artery in the tilapia than in the zebrafish 1.5.3 Objectives of the study There are two main objectives in this study: 1) Characterization of vtg multigene family in the zebrafish...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
Ngày tải lên :
16/02/2014, 09:20
... confirmed by Lin-54 binding to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86] ChIP experiments on the Cdc2 gene also provided insights into the association of other cell ... In G0, proteins appear to bind to the CDE ⁄ CHR, as monitored by in vivo footprinting According to these early experiments all binding is lost in G2 ⁄ M In contrast, constitutive binding to the ... proteins with the promoter E2F4 binding during the cell cycle coincides with the binding of p107 or p130 [61,85] Another E2F site distal to the E2F ⁄ CDE acts as an activating element binding...
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Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
Ngày tải lên :
23/03/2014, 15:21
... of E187 remained the same, but the pKa of E399 clearly increased Actually, the substitution of R97 by methionine or lysine resulted in the same increment in the pKa of E399 (Table 2) Therefore, ... function as the catalytic proton donor Thus, interactions between E399 and Y331 or R97 may be key elements in the determination of Sfbgly50 pH optimum The role of arginine in the modulation of the b-glycosidase ... the apolar side chain of F331 Thus, the increment in the pKa of E399, in addition to it being closer to E187, may have resulted in a large shift in the pKa of E187 Changes in the dielectric constant...
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Investigating the transcriptional regualtion of the stevor multi gene family in plasmodium falciparum
Ngày tải lên :
13/10/2015, 16:41
... There are several classes of drugs used for the treatment of malaria: 4aminoquinolines, arylaminoalcohols, 8-aminoquinolines, artemisinines, antifolates and inhibitors of the respiratory chain ... modifications in the protocol future experiments will allow the elucidation of the lengths of these 5‟UTRs Repeating these transfections and following the suggested modifications in the protocol of the ... cytoadherence of infected erythrocytes to many different host cells on the vascular lining during the blood stage of the infection, since it is expressed on the surface of erythrocytes in knob-like...
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The Role of Genetically Modified Organisms (GMOs) in Beverage Production
Ngày tải lên :
25/10/2013, 21:20
... throughout the world bears little resemblance to teosinte, the original ancestor of corn The newer techniques involving genetic engineering, on the other hand, allow for the transfer of a few genes in ... create signiÞcant changes in the genetic makeup of plants and animals due to the random recombination and sorting of thousands of genes As a result of intervention by people, the hybrid seed corn ... Finally, the food and beverage industries hope that the next generation of GM products will deliver compelling consumer beneÞts THE FUTURE OF GENETICALLY MODIFIED FOODS The next generation of...
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Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx
Ngày tải lên :
07/03/2014, 03:20
... helix of the third cluster of CHCs is part of the inter-domain segment and the other helix lines the boundary between the major domain and the N-terminus (Fig 1) Therefore, the formation of the ... at the value determined from the amino acid sequence (45 320 Da) Measurement of the Kd of PLP binding equilibrium PLP binding equilibria were analyzed taking advantage of the protein intrinsic ... explain the increase of the dissociation constant of the monomer–dimer equilibrium determined by the mutations The results obtained in the present study also show that the monomeric form of the...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
Ngày tải lên :
08/03/2014, 08:20
... of GSK3 (using Li or SB-415286) appears to be sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinase potentiates insulin action in muscle of insulin-resistant ... cell line, HT-29, and promote the transcription of the interleukin-8 gene [42] In line with previous studies, Fig 7A shows that Li induced the phosphorylation/activation of p38 MAP kinase in L6 ... GSK3 inhibition improves insulin responsiveness in muscle of insulin resistant animals it had no insulin potentiating effect in skeletal muscle of lean animals [39,41] To assess whether chronic inhibition...
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The Role of Gestures and Facial Cues in Second Language Listening Comprehension pptx
Ngày tải lên :
10/03/2014, 05:20
... placement in the IEP was determined on the basis of an in- house placement test of listening, reading, and writing skills (reliability coefficients for the listening and reading sections of this ... including instructions at the beginning, the listening comprehension task, and completion of the questionnaire The questionnaire was completed after the listening task so as not to bias any of the ... people, pointing out that in such circumstances visual cues would be of little to no value This is a very specific interpretation of the meaning of lecture Having examined over 20 definitions of the...
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Bringing the Hidden Giants to the Footlight: the Role of Savings and Retail Banks in Increasing the Level of Access to Financial Services docx
Ngày tải lên :
15/03/2014, 10:20
... bring their money into the formal financial system, instead of relying on informal circuits In designing their products, savings banks take into account the special needs and limitations of lowincome ... businesses, the traditional target client group of savings banks By capturing and channelling more of the remittances into the financial system and intermediating the flow of the resources, savings ... disbursing of loans and collecting loan repayments Another promising track for savings banks is to invest their savings deposits in microfinance institutions by specialising 18 Microfinance Information...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
Ngày tải lên :
17/03/2014, 17:20
... > h from the start of glucose infusion For the calculation of glycogen C6 changes, data acquired within h of the start of the glucose infusion was not included to avoid any in uence of transient ... the initial rate of label incorporation into glycogen C6 relative to the rate of label incorporation into glycogen C1 reflects the relative isotopic enrichment of Glc6P in the C6 relative to the ... should the tail vein fail, which was the case in only three animals, the data of which were included After the lines were inserted, pentobarbital was infused continuously at 4.8–6.0 mgÆkg)1Æh)1 The...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... Tyr These mutations may in uence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme for PAA and derivatives thereof, while maintaining the ... aromatic phenylalanines and the phenyl ring of the substrate play an important role in substrate binding The large effect of the mutations on the acylation rate indicate that these residues are ... results indicate that mutating bF57, which is located ˚ at the bottom of the binding pocket at A from the nucleophilic serine, does in uence the rate of formation of the acyl-enzyme, as judged by the...
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Báo cáo Y học: Cloning of the manganese lipoxygenase gene reveals homology with the lipoxygenase gene family doc
Ngày tải lên :
18/03/2014, 01:20
... alanine and three arginine residues, which might explain the low number of alanine and arginine residues in the two native Mn-LOX compared to the number of these residues of their deduced precursors ... [39] The homology of Mn-LOX and the LOX gene family included the two a helices of the latter that contain the four Fe(II) ligands These ligands are two His residues found in a helix (and in the ... determine the impact of deleting one or two amino acids in a helix on the metal center and on the catalytic properties The corresponding studies with insertion of one or two amino acids into the...
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