... level andthe urinary excretion of 8OHdG can be considered as a measure ofthe whole-body oxidativestress [20-22] The presence of 8OHdG in urine seems to originate mostly from the oxidation ofthe ... with the concentrations ofthe particle-related variables OC, NO x , and possibly the particulate copper content The increase oftheoxidativestress marker was accompanied by an increase of urinary ... (Institute for Work and Health) for their help in the metal/creatinine analysis, and comments on the manuscript The medical team ofthe Institute for Work and Health (Prof Marcel-André Boillat, Dr...
... position 13 ofthe other subunit [25,26], yielding a covalently bridged dimer The 4170 oxidized and reduced forms ofthe Y74C mutant had very different thermal stabilities While the stability ofthe ... and in the presence of denaturants [27] Extending these studies, we examine here the effect of increasing the bulk ofthe residue at position 74 Surprisingly, the Y74W mutant exhibited loss of ... across the dimer interface, while T75 interacts with N10 and E97 ofthe second subunit The backbone NH group of R98 forms a hydrogen bond with the backbone CO of F102 Furthermore, the orientation of...
... NO andthe SO anion [21] In the absence of L-arginine [19] and in sustained settings of low antioxidant status [20], the intracellular production ofthe SO anion increases The elevation ofthe ... levels of NO [22] Within a cell, the actions of NO are dependent on its levels, the redox status ofthe cell, andthe amount of metals, proteins, and thiols, amongst other factors [19] Since the ... sperm capacitation, and fusion ofthe sperm membrane andthe oolemma are especially dependent on the presence of ROS [4,60] On the other hand, inappropriately high levels of ROS produced by spermatozoa...
... patterns of B2wt andof B1 ⁄ B2 chimeras reflect their agonist-inducible internalization The segment between the NPXXY motif andthe conserved cysteine represents one ofthe regions with the highest ... influences the formation of a helical structure These authors proposed that activation ofthe receptor, and subsequently ofthe G protein, leads to a change in the environment of helix resulting in the ... to enhance their adherence Expression vectors The sequence ofthe human B2wt starting with the third encoded methionine [9], the sequence ofthe human B1wt, truncations and chimeras of both were...
... rhythm The stressed syllables are like the beats ofthe metronome: regular, loud, and clear The unstressed syllables between the beats are shortened, obscured, and joined together 26 The pattern of ... sweater Notice that the pitch of your voice rises at the major sentence stress, the first syllable ofthe word ―sweater‖, and falls over the second syllable of this word The pitch ofthe entire sentence ... important word in the sentence by giving it emphatic stress - Sentence stress marks the end ofthe sentence by giving the strongest stress to the last stressed syllable with the help of falling or...
... 4A,B), and its role during oxidativestress is further confirmed by the use of low concentrations of benzamide Preincubation of cells with benzamide prevented the peak activity observed during oxidative ... known to play an important role under oxidativestress [21]; however, there is no report on theroleof PARP in D discoideum development We have investigated theroleof PARP in D discoideum development ... death in response to oxidativestressand hence are used as therapeutic molecules to control oxidative stress- related diseases [12] However, the consequences ofthe blockade of cell death by PARP...
... As the pKa values ofthe catalytic residues determine the pH optima, the ionization constants (pKa) ofthe catalytic glutamates (E187 and E399) in the ES complex and in the free enzyme (E) of ... the expression ofthe recombinant b-glycosidases Lysis of induced bacteria Fig Schematic representation ofthe Sfbgly50 active site E399 is the nucleophile and E187 is the proton donor Y331 and ... b-glucosidase affected the rate constant ofthe glycosylation step and also changed the enzyme pH optimum [14] Despite that, the effect ofthe tyrosine on the pKa values ofthe b-glycosidase catalytic glutamates...
... urea-induced and GdnHCl-induced changes in the secondary structure ofthe proteins, we analyzed the UV CD spectra of MS2 and VLPs in the absence and presence of 5.0 m and 9.0 m urea and 3.5 m and 5.0 ... determined the effects on stability of genetic fusion ofthe two subunits ofthe dlFG dimer using the variant named 2CTdlFG, which takes advantage ofthe physical proximity ofthe N-terminus and C-termius ... Effect of pressure on the coat protein in the presence of bis-ANS for (n) dlFG and (e) sc-dlFG and in the absence ofthe probe for (m) dlFG and (r) sc-dlFG, was analyzed at room temperature The...
... for explaining the determinants of economic growth The focus on labor and capital as the primary factors of production, andthe general exclusion or trivialization oftheroleof knowledge, was ... skilled labor, and technology The neo-technology theories focused on theroleof R&D andthe creation of new economic knowledge in shaping the comparative advantage and flows of foreign direct ... function ofthe number and concentration of permanent links 199 Dispersion as a function ofthe number and concentration of permanent links 200 Expertise as a function ofthe number and concentration...
... (ADC) on thethe microcontroller, streamed to PC software Position sensors were attached to the thumb and forefinger, the wrist andthe base ofthe object, to enable accurate Saunders and Vijayakumar ... random unpredictable delays to the hand controller In contrast to experiments and 2, where the control ofthe hand was repeatable and predictable, this experiment was designed to examine therole ... briefly before the start ofthe first trial, with full feedback sensibility, so that they could get used to the control mechanism ofthe hand Subjects performed the four blocks ofthe experiment...
... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... matrix and promote healing [24] In this study, we have documented the relative efficiency of bone growth factors FGF-2, TGFb, and PGE2 markedly enhanced the synthesis ofthe total protein content of ... proliferation and mineralization [2,26-29] These two stages are the result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization...
... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... matrix and promote healing [24] In this study, we have documented the relative efficiency of bone growth factors FGF-2, TGFb, and PGE2 markedly enhanced the synthesis ofthe total protein content of ... proliferation and mineralization [2,26-29] These two stages are the result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization...
... equality, the rule of law and respect for and protection of human rights […] Recognizing the need to strengthen the important roleofthe United Nations in the prevention of violent conflicts, and ... emerged from the growing realization ofthe need to put the poor and all aspects of their lives and means of living at the centre of development and humanitarian work, while maintaining the sustainability ... and development activities, and requests the Secretary-General to submit a detailed review ofthe capacity ofthe United Nations system in the context ofthe report on the implementation of the...
... increased the expression ofthe anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit molecules into the DC nucleus Furthermore, we found that the inhibition of NF-κB by ... (A) The optical density of each band was divided by that ofthe β-actin band, andthe ratio at h was set at 100% (B) Fig NF-κB involvement in the taxol-induced effects on dendritic cells (DCs) The ... future study, the knockdown study of Bcl-xL using siRNA may further confirm their roleof taxol-treated DCs Although the expression of Bax was increased in TaxolDCs, the expression of Bax occurred...
... to either the inhibition of GSH synthesis or increased utilization of GSH for detoxification of toxicant induced free radicals [33] The decrease in SOD, blood GSH and GSH-Px suggests that the ... 15 16 17 18 19 20 21 22 Exacerbated responses to oxidativestress by an Na+ load in isolated nerve terminals: theroleof ATP depletion and rise of [Ca2+]i J Neurosci 2000, 20, 2094-2103 Chow CK, ... [24] Similarly, GST catalyzes the conjugation ofthe reduced glutathione to electrophiles and protects cellular components from oxidative damage [16] Increased activity of GST was reported in Drosophila...
... Finally, the comparison between the different behaviour ofthe species tested suggests that resistance to the action of aerosols is the result ofthe strength ofthe structures protecting the leaf, and ... illustrate the results of a number of experiments the aim of which was to measure the toxic- ity ofthe various components of polluted sea spray, both in isolation and in synergy MATERIALS AND METHODS ... collected both near the coast and 2-3 km inland Samples of bulk deposits were taken from pine trees (Pinus pinea L) from the section ofthe crown exposed to the sea and also from the opposite side...