the role of muscular force and fatigue in stress fractures

... explain the DpKa of E187 in the Y331F mutant, because in the R97K and R97M mutants the same increment in the pKa of E399 did not change signicantly the pKa of E187 This suggests that in the Y331F ... Taking into account the experimental errors, the pKa of E187 remained the same, but the pKa of E399 clearly increased Actually, the substitution of R97 by methionine or lysine resulted in the ... determinations using mM MUbglc as substrate The pH stability of the recombinant Sfbgly50 (n) was checked by incubating the enzyme in the same buers for an equal length of time and determining the...

Ngày tải lên: 23/03/2014, 15:21

... trees of P pinea, P tobira, Q ilex and A opalus growing When interpreting the results of the chemical analysis of rainwater and deposits it is necessary to bear in mind the interactions between the ... However part of the chlorides absorbed by the needle are released during rinsing In the rainwater samples there can be interferences with atmospheric dust and dry deposits of nonmarine origin In any ... those with the greatest impact the system because of the amount of salt and surfactants they transport, occur rather infrequently within the total number of samples examined Finally, the ratio...

Ngày tải lên: 08/08/2014, 19:21

... identify factors influencing the use of RBPs among staff nurses in the Canadian province of Newfoundland and Labrador with the specific aim of understanding the role of P&Ps in promoting research ... placed nurses in this study in the persuasion stage of adoption, overall In other words, they were aware of and believed in the value of the practices but were not using them to make clinical decisions ... understanding of the factors that influence nurses' use of RBPs, and the role that P&Ps may play in promoting research utilization in nurses Our findings suggest nurses use P&Ps to guide their...

Ngày tải lên: 11/08/2014, 05:22

... COPD [106] Vitamin A and B Vitamin A (retinol and carotens) plays an important role in several functions of the human body including vision, bone and skin health, and further has an innate antioxidant ... protective role of vitamins against COPD but did not measure vitamins in serum or with a FFQ Therefore conclusions about the special role of any vitamin in COPD could not be obtained Vitamin D and COPD ... assessment of vitamins has the disadvantage that they represent the more recent intake, and for some vitamins such as vitamin C, the levels in peripheral blood are not representative of intake and not...

Ngày tải lên: 12/08/2014, 13:22

... and plasticity, brain growth, neuron repair and remodeling, and even maintaining the normal function of this important organ (Dietschy and Turley, 2001; Herz and Bock 2002; Koudinov and Koudinova, ... orthologs and is referred to as the NPC1 domain Within NPC1 there is a large, cysteine-rich luminal loop (amino acids 855–1098), which contains a ring-finger motif that may mediate protein– protein interactions ... other cholesterol binding proteins, such as sterol carrier protein 2, caveolin, StAR protein, or MLN64 The structure of the apo form of bovine NPC2 has been solved, revealing an immunoglobulin-like...

Ngày tải lên: 14/09/2015, 14:06

Ngày tải lên: 10/05/2016, 01:49

... distortions The diminishing effects of initial conditions over time is shown by the convergence of PRIN1 and PRIN2 to the zero line Finally, an interesting question is whether these results continue ... aspects of transition Ickes (1996) discusses some of the consequences of not including initial conditions in the analysis of reforms and performance in the context of transition In his review of the ... estimates of the relative importance of initial conditions and policies in determining performance, as measured by growth and inflation In this section we also study the time profile of output and inflation...

Ngày tải lên: 30/08/2016, 07:40

... binding was determined in the presence of lm unlabeled agonist and subtracted from the total binding to calculate the specific binding Internalization of [3H]BK and [3H]DAK To determine the internalization ... provide insight into the receptor specificity of the B2wt internalization motif The resulting data also hint at a receptor specific role of the putative helix in G protein activation and interaction ... expressed in percentage of total binding, decreasing the binding affinity of the FEBS Journal 272 (2005) 129–140 ª 2004 FEBS A Faussner et al Role of helix and C-termini in bradykinin receptors Fig Schematic...

Ngày tải lên: 07/03/2014, 16:20

... of GSK3 (using Li or SB-415286) appears to be sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinase potentiates insulin action in muscle of insulin-resistant ... greater than that by insulin Since the activity of GS depends on the relative activities of GS kinases and PP1G, inhibiting GSK3 (one of the principal GS kinases) will shift the balance towards ... by insulin Regulation of GS activity To establish the importance of GSK3 inhibition on GS activity we monitored the effects of insulin, Li, SB-415286 and wortmannin (a PI3K inhibitor) on the incorporation...

Ngày tải lên: 08/03/2014, 08:20

... Tyr These mutations may in uence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme for PAA and derivatives thereof, while maintaining the ... maintaining the structure of the binding site given the short distances of ˚ ˚ 3.5 A and 3.9 A between bF57(CZ) and the substrate binding residues bP22(CB) and bF24(CE2), respectively Changing ... residues and interactions in uence the performance of PA in the formation of semisynthetic b-lactam antibiotics The X-ray structure of the complex of PA with PAA shows that the acyl binding site of...

Ngày tải lên: 17/03/2014, 23:20

... are unsure of the mechanism of DNA-induced stabilization of the protein, and whether it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect The increased ... urea-induced and GdnHCl-induced changes in the secondary structure of the proteins, we analyzed the UV CD spectra of MS2 and VLPs in the absence and presence of 5.0 m and 9.0 m urea and 3.5 m and 5.0 ... the role of protein–protein and protein–RNA interactions in virus stability is not completely understood, and we have investigated these questions in this work We sought to determine the role of...

Ngày tải lên: 30/03/2014, 11:20

... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... examined and photographed the cells on a Zeiss Axioskop using 20× Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the ... We then visualized the mineralizing cells with 2% Alizarin Red After rinsing in distilled water and air drying the samples, we mounted the coverslips on microscope slides using Fluoromount and...

Ngày tải lên: 20/06/2014, 04:20

... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... examined and photographed the cells on a Zeiss Axioskop using 20× Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the ... We then visualized the mineralizing cells with 2% Alizarin Red After rinsing in distilled water and air drying the samples, we mounted the coverslips on microscope slides using Fluoromount and...

Ngày tải lên: 20/06/2014, 07:20

... viability These findings provide a basic rationale for the use of taxol in DC immunotherapy and insights into the development of combinational DC therapies with other anticancer drugs that not harm the ... survival and maturation of DCs [11,17,20] To investigate further the mechanisms of taxol-induced changes, the viablity of TPCK, an inhibitor of the serine protease that inhibit NF-κB signaling pathway ... major cytokines involved in immune responses Importantly, taxol increased the expression of the anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit molecules into the DC...

Ngày tải lên: 07/08/2014, 23:22

... the decision of continuing the ongoing therapy or stopping and redirecting the patient to other salvage therapies Our study also supports the idea of routine use of PET or preferably PET/CT in ... Kim and colleagues accepted the cut off SUVmax value as 2.5 in their study at which the role of PET/CT was investigated, and they found out the Page of sensitivity of the technique 40% [12] In ... uptake in our study It is an important limitation that the number of patients is small especially in the first group, the number of studies in the literature investigating the role of PET in the...

Ngày tải lên: 09/08/2014, 02:20

... IL-1 and TNF, rather than a 'cytokine cascade' with either of these cytokines controlling the production of the other (Figure 4a) Both Enbrel and the neutralising anti-IL-1β antibody decreased the ... cytokines and/ or MMPs or indirectly due to cytokine-mediated inhibition of the production of growth factors [16] The majority of studies of the regulation of cytokines and destructive enzymes in ... by then, large quantities of these MMPs have already been produced, thus blurring the results In contrast, the PCR findings reflect the state of the cells days after addition of the Enbrel and/ or...

Ngày tải lên: 09/08/2014, 08:23

... at the same three sites as the symptomatic subjects prior to an injection of cc of1 % lidocaine in the left thigh, and then re-evaluated for PPT within minute of the injection The purpose of the ... thresholds in uninjured tissues, and explained the finding as the expression of an abnormal processing of nociceptive information in the brain and spinal cord [2-7] Others have postulated that chronicposttraumatic ... contrast, the increase in cervical range -of- motion observed in the symptomatic cohort following the injections suggests that the initial finding of reduced motion was either a result of peripherally...

Ngày tải lên: 10/08/2014, 10:20

... variation in the period of ischaemia, the muscle temperature during ischaemia and the period of reperfusion allowed for manifestation of the reperfusion injury Idstrom [12] utilised a period of 2, and ... littermates Thus, engraftment of BMMC into Wf/Wf mice restores the susceptibility of skeletal muscles to IR injury irrespective of the other abnormalities in these mice The role of mast cells has not ... and drafted the original manuscript WAM participated in the design of the study AM participated in design of the study and performed morphological analyses All authors provided intellectual input,...

Ngày tải lên: 11/08/2014, 08:21

... cyclin binding motifs, one at the N- and the other at the C- terminus [20] p21/waf1 (mut) is mutated at the N-terminus and is therefore still able to bind to cyclins through the C-terminus, making ... terminus and the other at the C terminus [20] p21/waf1 interacts with both cyclins and cdks, in contrast to the INK family CDKI members, which only bind to cdks [20] Interestingly, p21/cyclin ... represents the nucleolus, whereas the lighter blue staining represents the nucleoplasm As expected, the intensity of staining for cyclin D2 and p21/ waf1 was increased in HTLV-1 infected T-cells When the...

Ngày tải lên: 13/08/2014, 13:20

... due to the lack of the linker region tethering together two molecules that are simply within range of the linker arm and possibly not involved in a genuine interaction (41) 10 Materials and Methods ... has the potential to influence the DNA binding activity of Ku (51) To further understand the role of the C-terminus of Ku80, we created two mutants The first mutant contains a truncated form of ... Preliminary work was performed to determine the elution volume of Ku70/80C and Ku80CTR independent of the other protein Individual proteins were pre-incubated in running buffer and applied to the...

Ngày tải lên: 24/08/2014, 11:02