the role of mesenchymal stem cells in tumor microenvironment

... effects against the cancer cells were studied in vitro In vivo MSC/Fluc migration in mice having thyroid or breast cancer xenografts was evaluated after systemic injection Rluc activity of CAL62/Rluc ... candidate for cancer therapy The purpose of this study was to track the migration of MSCs in tumor- bearing mice; MSCs were also used as drug delivery vehicles Human breast cancer cells (MDA-MB-231) ... MSC/Fluc and naïve MSCs in migration assay DOX treated MSCs migration was not decreased compared than MSCs Migration of the systemically injected MSC/Fluc cells into tumor xenografts (thyroid

Ngày tải lên: 15/01/2020, 09:12

... a role in the induction and maintenance of the neuronal cholinergic phenotype in the central nervous system [27] However, conflicting results have implicated BMP9 as either an angiogenesis inducer ... differentiation Using adenovirus-mediated expression of the 14 types of BMPs we demonstrated that BMP9 is one of the most potent BMPs in inducing osteogenic differentiation of mesenchymal stem cells (MSCs), ... stable cell line expressing the full coding region of mouse BMP9 was established in HEK-293 cells by using the piggyBac transposon system The biological activities and stability of the conditioned

Ngày tải lên: 15/01/2020, 20:53

... understanding of the mechanisms of reprogramming at the multicellular and single-cell levels Characterization of iPSC lines Reprogramming of somatic cells is hindered by the heterogeneity of the derived ... plakophylin-2 (PKP2) mutation and are in the process of modeling the disease in vitro [133] Another study examining the same disorder used iPSC-CMs to uncover the role of a different mutation in the ... of reprogramming of primary fibroblasts [129, 130] Keratinocytes Keratinocytes, the most abundant cell type in the epidermis, are involved in the protection of the skin and Abou-Saleh et al Stem

Ngày tải lên: 19/03/2021, 23:02

... replace the lost cardiomyocytes in the injured heart represents a promising therapeutic approach in this context [2] Stem cell therapy has emerged as a novel strategy for the treatment of ischemic ... disease during the past decade Various stem cell types have been used for the repair of the damaged heart [2–4] Noteworthy benefits are revealed in the regeneration of cardiomocytes following the transplantation ... (MSCs) are capable of differentiating into cardiomyocytes under appropriate conditions both in vitro and in vivo [6] In spite of this, the transdifferentiation efficiency of these cells is extremely

Ngày tải lên: 04/12/2022, 15:12

... stem cells, including embryonic and adult stem cells, and we provide a detailed discussion of the properties of induced pluripotent stem cells (iPSCs) We also present and critically discuss the ... after, in 1998, the first human embryonic stem cell was derived Those cells remained the only pluripotent stem cells at the disposal of researchers until 2006, when Shinya Yamanaka identified the ... al Stem Cell Research & Therapy (2018) 9:201 Page of 31 Fig The integrating reprogramming method using viral transduction The first method developed to deliver OSKM factors involved the use of

Ngày tải lên: 18/07/2023, 20:04

... rise to other kinds of cells [1] Stem cells can be classified on the basis of their origin and potential to differentiate Based on origin, these cells are of two types: embryonic stem cells (ESCs) ... transfected into MSCs results in improved viability of the cells in infarcted hearts, thereby preserving the contractile function along with improving the paracrine action of the cells [83] Similarly, ... for their paracrine effects in reducing inflammation [188] and promoting growth of the surrounding cells [268] MSC injection promotes the recruitment of CPCs and helps in the improvement of myocardium

Ngày tải lên: 04/12/2022, 15:30

... mitosis in culture, providing the clearest evidence of in vitro oogenesis In addition, the PSCs showed positive alkaline phosphatase staining, and the intensity of the staining was stronger in the ... resulted in the transformation of induced embryonic stem cells (ESCs) into PGC-like cells The PGC-like cells then went on to contribute to the pool of OLCs in the reconstituted ovaries These studies ... pipetting The cells were maintained at 38.5°C in an atmosphere of 5% CO2 in air After selection, 1-2×104 cells were plated in one well of a 24-well gelatin-coated plate (Corning) Half of the culture

Ngày tải lên: 16/12/2017, 04:38

... cell-based therapies as they are capable of differentiating into neurogenic, adipogenic, myogenic, and osteogenic lineages [1-3] The fate of the stem cells is influenced by the microenvironment in which ... seeding on polyacrylamide substrates The major and minor axes of the cells were computed from the moments up to the second order of the thresholded binary image of the cell using NIH ImageJ; the ... easily modified by altering the density of cross-links in the gel Increasing the concentration of either the amount of acrylamide monomer or bis-acrylamide cross-linker resulted in a gel with a higher

Ngày tải lên: 15/01/2020, 00:46

... have often demonstrated a >100-fold increase in tumorigenic potential, compared to the remainder of the cells in the tumor Furthermore, tumors that form from these cancer stem cells are indistinguishable ... cancer stem cells are indistinguishable from the human tumors in which they originate, indicating that the tumorinitiating cells are stem cell-like in their ability to selfrenew and give rise to ... subclones thereof, are the most likely perpetrators of invasion and metastasis [6,62] Recent findings have shown the existence of activated and quiescent repertoires of stem cells in established tumor

Ngày tải lên: 05/11/2020, 01:11

... ABSTRACT The main purpose of this research is fabricating gelatin nanofibers and investigating the application of nanofibers for skin regeneration First, the gelatin nanofibers were fabricated by using ... spindle shape Acid acetic was used to dissolve the gelatin, it makes the medium acidic so that this is the main factor influencing the results in the study [12] On the other hand, although the ... gelatin showed an optimum combination of hydrophilic and biodegradability and also maintained the structural integrity of the scaffolding Also, the interaction and proliferation of hMSCs are the

Ngày tải lên: 22/12/2020, 22:04

... (Bmi-1), a member of the polycomb family of transcriptional repressors, is involved in cell cycle regulation, self-renewal of stem cells and cell senescence7,8 Bmi-1 inhibits the p16INK4a/Rb and p19AFR/p53 ... differentiate into other cell types Stem cells also secrete paracrine cytokines and improve microenvironments14 Amniotic membrane mesenchymal stem cells (AMSCs) are a better prospect for cell therapy ... prevent aging-associated degenerative diseases by migrating into organs and maintaining redox balance To investigate the potential of AMSCs, Bmi-1-deficient (Bmi-1−/−) mice were injected intraperitoneally

Ngày tải lên: 19/11/2022, 11:39

... 40] The expression of AQP1 decreased significantly after induction of ANP Fig Tracking of infused FITC-albumin in the small intestine and the mesentery of the small intestine 24 h after the establishment ... reduction of damage to the small intestinal capillary endothelial barrier, and increased expression of AQP1 in the small intestine Keywords: Mesenchymal stem cells, Acute necrotizing pancreatitis, Intestinal ... venule endothelial cells This induced edema within the small intestine, resulting in the impairment of small intestinal microcirculation and injury to the small intestine in ANP Studies have shown

Ngày tải lên: 24/11/2022, 17:56

... ventricles of the remaining rats were weighed to calculate the ratio of left-ventricular weight to body weight The size of the infarct was obtained by calculating the percentage of the infarcted ... embryonic-like mesenchymal stem cells (vselMSCs) Moreover, the number of these vselMSCs in the infarct related artery was much higher than in those from the peripheral vein This is the first report of a ... could decrease the infarct size, and prevent post-infarction remodeling of the heart,21 but the role of HIF-2α in cell-autonomous VSEL maintenance remains unknown The present study was the first to

Ngày tải lên: 04/12/2022, 10:38

... evaluating the tumor suppressive effects of MSCs on the in vivo progression of HCC, and to investigate the possible role of Wnt signaling in tumor tissues by assessing the gene expression profile of ... mimicking the one of resident cells in the host tissue Insults trigger the release of chemokines from the endothelium inducing adhesion and migration of circulation BMDC into the liver parenchyma The ... that link MSCs to the targeted inhibition of tumor cells Despite their distinct origins, stem cells and tumor cells share many characteristics[15,16] In particular, they have similar signaling...

Ngày tải lên: 10/08/2014, 10:21

... measurement of the abundance of CD4+ T cells, CD8+ T cells, and CD3- CD49b+ NK cells in the spleens of BALB/c versus scid mice (n = 10 per group) "Other WBCs" refers to the fraction of spleen cells ... NK cells and/or professional APCs confer innate resistance to HSV-1 infection via the secretion of IFN-γ at early times p.i The following study was initiated to determine if NK cells provide innate ... in HSV-1 infected tissues just 24 hours post inoculation (p.i.; Fig of Ref [31]) T cells, NK cells, and professional antigen-presenting cells (APCs) are the primary IFN-γ-producers in the body...

Ngày tải lên: 19/06/2014, 08:20

... measurement of the abundance of CD4+ T cells, CD8+ T cells, and CD3- CD49b+ NK cells in the spleens of BALB/c versus scid mice (n = 10 per group) "Other WBCs" refers to the fraction of spleen cells ... NK cells and/or professional APCs confer innate resistance to HSV-1 infection via the secretion of IFN-γ at early times p.i The following study was initiated to determine if NK cells provide innate ... in HSV-1 infected tissues just 24 hours post inoculation (p.i.; Fig of Ref [31]) T cells, NK cells, and professional antigen-presenting cells (APCs) are the primary IFN-γ-producers in the body...

Ngày tải lên: 20/06/2014, 04:20

... proteoglycans are involved in rendering the disease chronic [16,17] Beyond the 100% incidence of arthritis, another major advantage of the AIA model is that the time point of induction of arthritis ... AIA is dominated by cells of the innate immune system [19], and the exacerbation of arthritis in CD25-depleted mice could be due to a lack of suppression of these cells by Treg cells In accordance ... is known, allowing manipulation of CD4+CD25+ Treg cell number in vivo at defined stages in the disease Using depletion of CD25-expressing cells or transfer of CD4+CD25+ cells, in the present study...

Ngày tải lên: 09/08/2014, 06:22

... through the inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced ... levels of proinflammatory cytokines in the articular joint These are produced by synovial lining cells, macrophages and the chondrocytes themselves further exacerbating cartilage degrading and ... combination of both (1) lead to the production and accumulation of high levels of pro-inflammatory cytokines (2) in the joint These trigger the production of additional pro-inflammatory cytokines...

Ngày tải lên: 12/08/2014, 14:22

... on the perspective of cellular therapy by intravenous stem cells infusion The participation of stem cells in several vascular diseases pathogenesis was first proved with haematopoietic stem cells ... and the adherent soft tissue was removed The proximal and distal ends of the femur were excised at a level just into the beginning of the marrow cavity Whole marrow plugs were obtained by flushing ... Figure Mesenchymal stem cells used during this study Mesenchymal stem cells used during this study Typical morphological aspects of mesenchymal stem cells observed through culture flask (A) Mesenchymal...

Ngày tải lên: 12/08/2014, 18:20

... resulting in the killing of 39 Chapter 1: Introduction these infected cells Under these circumstances, the dying cells, CD8 T -cells and other APCs such as macrophages and DCs within the inflammatory ... containing large spherical mitochondria which, in the living state in vitro, are continually elongating, retracting, and reorienting themselves, leading to a wide variety of cell shapes (Steinman ... 1995) They then induce cytolysis of infected cells either by the granule exocytosis pathway through co-ordinated delivery of perforin and granzymes into target cells, or by upregulation of FasL...

Ngày tải lên: 09/09/2015, 18:58