the role of hepatocyte growth factor

... ROLE OF HEPATOCYTE NUCLEAR FACTOR 4A IN THE KIDNEY DULESH NIVANTHA PEIRIS (B.Sc Honours., NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF PHYSIOLOGY YONG ... modulate the expression or activity of the transcriptional factors we might be able to affect the outcome of the disease Several transcriptional factors are known to be expressed in the kidney ... shows the average of the four acute tubular cases expressed as a percentage together with the control 23% of the nuclei in the acute tubular cases show strong staining compared to 0% in the control...

Ngày tải lên: 16/10/2015, 15:38

... activation of HGFA Arrow denotes the site of cleavage by plasma kallikrein EGF, epidermal growth factor of the other proteases on HGF are very weak in vitro, they may be stimulated by a cofactor(s) ... Activation of the zymogen of hepatocyte growth factor activator by thrombin J Biol Chem 268, 22927–22932 Miyazawa K, Shimomura T & Kitamura N (1996) Activation of hepatocyte growth factor in the injured ... activation of hepatocyte growth factor ⁄ scatter factor EMBO J 11, 4825–4833 Shimomura T, Miyazawa K, Komiyama Y, Hiraoka H, Naka D, Morimoto Y & Kitamura N (1995) Activation of hepatocyte growth factor...

Ngày tải lên: 15/03/2014, 11:20

... Activation of the zymogen of hepatocyte growth factor activator by thrombin J Biol Chem 268, 22927–22932 11 Miyazawa K, Shimomura T & Kitamura N (1996) Activation of hepatocyte growth factor in the ... the cleavage sites of pro-HGFA and epitopes of the antibodies A-1 recognizes the light chain of active HGFA (hatched bar), but the precise position of the epitope is not known The intra-heavy-chain ... analysis of the cDNA for a human serine protease responsible for activation of hepatocyte growth factor J Biol Chem 268, 10024–10028 Lee SL, Dickson RB & Lin CY (2000) Activation of hepatocyte growth...

Ngày tải lên: 23/03/2014, 07:20

... subacute cutaneous lupus erythematosus (SCLE) lesions [52] Given the role of TNF as a strong mediator of inflammation, the high levels of serum TNF in active SLE, and the overexpression in renal ... still limited, Page of (page number not for citation purposes) there are a minimum of 28 cases reported at least in abstract form [56-61] and most of these are on the use of the chimeric monoclonal ... not only challenges the hypothesis, but even suggests that the reciprocal negative regulation of the two cytokines is not functional in active SLE Rather, the combined increase of both TNF and IFN-α...

Ngày tải lên: 09/08/2014, 10:22

... inhibited the degradation of GAG in the FGF8-treated joints by 33% (P = 0.036) These data indicate that the injection of FGF8 causes degradation of the ECM of the articular cartilage and release of ... Injection of FGF8 into the rat knee joints promoted the release of S-GAG from cartilage into the synovial fluid Following injection of FGF8, the weight of the patella was reduced Further study ... accelerated the degradation of the ECM The participation of synovial inflammation in the progression of cartilage changes at the clinical stage of OA is becoming increasingly obvious [1] A number of clinical...

Ngày tải lên: 09/08/2014, 10:23

... synthesis: role of the epidermal growth factor receptor cascade The mechanism of EGFR expression and activation Stimulation of airway epithelial cells with TNF-α causes EGFR expression (the extracellular ... concentrated in the epithelium of EGFR-positive than in the epithelium of EGFR-negative polyps It has been speculated that EGFR activation in polyps could occur via the release of TGF-α from Available ... to the degree of ‘activity’ of the disease Nasal polyp epithelium contains increased numbers of goblet cells [9] Specimens from healthy persons did not express EGFR strongly while four out of...

Ngày tải lên: 12/08/2014, 18:20

... mouse EC cells contributes to the derivation of the mouse ES cells The supporting layer of fibroblast and the conditioned medium were found to favor the maintenance of the EC cell lines10 1.1.2 Mouse ... require the supplement of basic fibroblast growth factor (bFGF) and transforming growth factor b (TGFb)/activin36 Mouse ES cells remain undifferentiated in the presence of leukemia inhibitory factor ... into the nucleus and result in the transcriptional regulation of their downstream targets Apart from the signaling pathways related transcription factors such as Stat3 and Smad1, another set of...

Ngày tải lên: 14/09/2015, 14:07

... certain members of the family of transcription factors called interferon-regulatory factors (IRFs) (Honda and Taniguchi, 2006) The role of IRFs first came into light during the study of interferon- ... on the promoter regions of the genes they regulate (Fujii et al, 1999; Panne et al, 2004) We inspected the promoter sequence of Rae1 using MatInspector of the Genomatix software suite and further ... activation of ATR and if so, it would further underline the role of ATR in the TBK1/IRF3 pathway Indeed, similar to the ATR-dependent phosphorylation of TBK1 (S172), the phosphorylation of IRF3...

Ngày tải lên: 13/10/2015, 15:54

... the structure of the inter-cysteine loops3 In the case of EGF-like domain, the conservation of disulfide-connectivity had also led to the conservation of the overall fold, thus the study of the ... the overall topology of the proteins, what then provide the information needed to generate the high-resolution structures of these proteins? These information come from the exact identities of ... sequence of statements of the source code Analogously, the “execution” of the folding code will result in the folding of the polypeptide chain into its native structure based on the overall balance of...

Ngày tải lên: 13/10/2015, 16:41

... approximately 15% of the total cell population in the liver, HSCs are located in the space of Disse between the hepatocytes and the endothelial cells in the liver (Fig 1.1) As the name implies, ... platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF) and endothelial growth factor (EGF) (Friedman, 2008c) 1.3 Transforming Growth Factor- β1 (TGF-β1) The TGF-β1 peptide belongs to the ... shown The band intensities of Snai1 and Cx43 were estimated using the ImageJ software and normalized to the β-actin loading control The numbers represent the intensities of the bands relative to the...

Ngày tải lên: 16/10/2015, 11:58

... accordance with the guidelines of the National Institutes of Health (Bethesda, MD, USA), as specified by the animal care policy of Hyogo College of Medicine Expression vector and preparation of liposomes ... sequence analysis of cDNA for human hepatocyte growth factor Biochem Biophys Res Commun 1989, 163:967-973 Zarnegar R, Michalopoulos GK: The many faces of hepatocyte growth factor: from hepatopoiesis ... the TSK/+ phenotype, because a lack of these cells prevented development of dermal thickening [31] Therefore, we examined the effect of HGF on the generation of IL-4 from CD4+ T cells HGF significantly...

Ngày tải lên: 09/08/2014, 08:22

... stability of HGF-CNP to focus further on the localized delivery with the receptor mechanism Acknowledgements The authors are thankful to the Director and the members of the Research Council of CSIR-Central ... enhance the differentiation; secondly, the liver being the homing organ for any foreign particles, the CNP upon reaching the liver is degraded to release the growth factor which can induce the differentiation ... knockout model [37] or the fibrolysis of ECM content [38] For better understanding we have compared the contribution of either HSC or MSC in the process of reversal of cirrhosis in the liver A significant...

Ngày tải lên: 11/08/2014, 00:23

... initiation of apoptosis through the mitochondrial pathway The key event in the mitochondrial pathway is the release of proapoptotic factors from the mitochondrial intermembrane space into the cytosol, ... (2007) Roles of epidermal growth factor family in the regulation of postnatal somatic growth Endocr Rev 28, 284–296 Henson ES & Gibson SB (2006) Surviving cell death through epidermal growth factor ... release of cytochrome c and second mitochondria-derived activator of caspase (Smac ⁄ DIABLO) allows for the formation of the apoptosome, a complex that enables the activation of caspases within the...

Ngày tải lên: 16/02/2014, 09:20

Ngày tải lên: 18/02/2014, 11:20

... mobility with respect to the rest of the structure Further studies of the receptor bound forms of the chimera and other related ligands will be necessary to define the nature of the interactions leading ... Several of these were identified in previous studies of EGF, and the backbone fold of the chimera is clearly similar to those of the EGF structures The presence of a backbone hydrogen bond from the ... Ribbon diagram of the secondary structure in residues 5–47 of the overall best structure of the chimera The three disulfide bridges are also shown, together with the sidechains of Asn16 and Arg41,...

Ngày tải lên: 22/02/2014, 07:20

... affected by a number of factors, including the openness of the capital account, the degree of euroization of the economies, the adequacy of monetary instruments and the effectiveness of monetary policy ... assessment should be an analysis of the credit growth on the basis of detailed underlying data, including the speed of the growth, breakdown of aggregate credit in terms of the borrower (households, ... guided by the nature of the risks associated with the nature of the credit growth (see Table for a mapping from various features of the credit growth to different types of risks, and Table for the...

Ngày tải lên: 06/03/2014, 08:20

... L4 be the quantity of input of the Ith labor service, measured in man- hours The rate of growth of the index of total labor input, say L, is: i — —— L — where v1 is the relative share of the lth ... P1 is the price of the lth category of labor services and P'i is its relative price The relative price is the ratio of the price of the lth category of labor services to the average price of labor ... for such a hypothesis, partly because of lack of relevant data They used the education of the total labor force in a state for the measurement of the quality of the labor force of individual industries...

Ngày tải lên: 06/03/2014, 21:20

... aspects of the income distribution We then turn to the relationship of education and economic growth Research into the economics of growth has itself been a growth area, but much of the research ... into the determination of growth A variety of factors clearly contribute, and these factors work to overcome any deficits in quality These other factors may also be necessary for growth In other ... on the Effects of Levels of and Growth in Years of Schooling To provide a basic representation of the association between years of schooling and economic growth on the most recent version of...

Ngày tải lên: 08/03/2014, 08:20

... play a major role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of transcription Ó FEBS 2004 Regulation of the presenilin ... appears to reverse the effect of either of the single mutations alone These results considered together with the effect of 3¢-deletions to +107 and +42 suggest that deletion of sequences downstream ... using the concentration of protein in the extract as an internal standard The activity of ()119/+178) PS1CAT in the presence of the empty vector was taken as 100% The values represented in the...

Ngày tải lên: 16/03/2014, 18:20

... modulate the sensitivity in the low micromolar range because the IC50 value of the dephosphorylated wild-type HRI was increased to 2.4 lm On the other hand, the IC50 value (5.5 lm) of the dephosphorylated ... phosphorylation at Ser51 of eIF2a is triggered Therefore, it is important to explore the role of autophosphorylation of the HRI protein in the intramolecular protein–protein interaction (between the NTD and ... located at the dimer interface and is thus trans-phosphorylated by the kinase domains of other subunits of HRI (Fig 5B) Unfortunately, because of the low ion score (i.e a measure of how well the observed...

Ngày tải lên: 28/03/2014, 23:20