the role of glioma stem like cells

... reported, the exact origin of these cells is still debated Do CSCs directly originate from normal stem cells, or are they the outcome of ovarian cancer cells that have gained stem cell properties? The ... establish tumors Taken together, the functionality of these clones suggested their identity to be that of cancer stem cells Generation of CSC from transformed stem cells vs tumor cell dedifferentiation ... fraction of stem cells termed as the side-population (SP) stem cells [9] The SP population was further found to be tumorigenic and withstood their identity as CSCs in all the classical stem cell...

Ngày tải lên: 20/06/2014, 07:20

... search for the presence of putative stem cells The analysis confirmed the presence of round, SSEA-4 and SOX-2-positive primitive oocyte -like cells in the ovarian surface epithelium These cells resembled ... possible The primitive oocyte -like cells in the ovarian surface epithelium of this patient might have persisted from the fetal period of life or developed from the putative stem cells in the ovarian ... VASA-positive cells (arrows) in the ovarian surface epithelium and cortex (A, B) Smaller cells with diameters of to μm in the epithelium (C-F) Bigger cells with diameters of 10 μm in the epithelium...

Ngày tải lên: 20/06/2014, 08:20

... fragment of the mRNA of the gene (from position to position 962 of the mRNA of the CaOvate), was EcoRI digested The digestion produced a 794-bp fragment that lacked 168-bp of the 5’ of the mRNA ... pattern in the Arabidopsis embryo sac [15] The two subfamilies, the one with CaOVATE, AtOFP6, AtOFP7 and AtOFP8 and the other with AtOFP1, AtOFP2, AtOFP3 and AtOFP5, have a significant number of common ... expression of the gene in the DAA fruit of the infiltrated plant compared to the expression in the DAA fruit of the WT The analysis of the CaOvate genomic sequences obtained from the two cultivars...

Ngày tải lên: 11/08/2014, 11:21

... application of stem cells which has been impeded by lack of efficient and straightforward ways to obtain the patient-specific stem cells Introduction 1.1 Pluripotent stem cells Pluripotent stem cells ... work in mouse EC cells contributes to the derivation of the mouse ES cells The supporting layer of fibroblast and the conditioned medium were found to favor the maintenance of the EC cell lines10 ... (BMP)37 The colony morphology of human ES cells is different from that of mouse ES cells The former cells form flat, sheet -like colonies, while the latter cells form round and island -like ones...

Ngày tải lên: 14/09/2015, 14:07

... type Hence it is likely that Ets elements play a major role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of transcription ... using the concentration of protein in the extract as an internal standard The activity of ()119/+178) PS1CAT in the presence of the empty vector was taken as 100% The values represented in the ... appears to reverse the effect of either of the single mutations alone These results considered together with the effect of 3¢-deletions to +107 and +42 suggest that deletion of sequences downstream...

Ngày tải lên: 16/03/2014, 18:20

... Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells ... study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing embryos revealed the uniqueness ... is the establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of...

Ngày tải lên: 23/03/2014, 07:20

... Percentage of PBMCs displaying p-STAT-3 does not correlate with the percentage of p-STAT-3 positive tumor cells in the glioma To determine if the level of p-STAT-3 positive cells in the glioma correlated ... both the tumor cells and the immune cells within the tumor microenvironment is initiated as proposed by Kortylewski [21] The cumulative response of activating the STAT3 pathway in the immune system ... designated as the number of CD4+CD25+FoxP3+ cells divided by the total CD4+ population Immunohistochemical analysis of p-STAT-3 in gliomas After formalin-fixed, paraffin-embedded sections of the gliomas...

Ngày tải lên: 18/06/2014, 15:20

... measurement of the abundance of CD4+ T cells, CD8+ T cells, and CD3- CD49b+ NK cells in the spleens of BALB/c versus scid mice (n = 10 per group) "Other WBCs" refers to the fraction of spleen cells ... the rate of KOS-GFP spread to the periocular skin An important caveat of the NK cell depletion experiments was that control IgG or anti-asialo GM1 had the unanticipated effect of prolonging the ... [56,57] Further study is needed to test this hypothesis Role of Stat in innate resistance to HSV-1 The biological functions of IFN-α/β and IFN-γ are dependent on the phosphorylation of Stat 1,...

Ngày tải lên: 19/06/2014, 08:20

... measurement of the abundance of CD4+ T cells, CD8+ T cells, and CD3- CD49b+ NK cells in the spleens of BALB/c versus scid mice (n = 10 per group) "Other WBCs" refers to the fraction of spleen cells ... the rate of KOS-GFP spread to the periocular skin An important caveat of the NK cell depletion experiments was that control IgG or anti-asialo GM1 had the unanticipated effect of prolonging the ... [56,57] Further study is needed to test this hypothesis Role of Stat in innate resistance to HSV-1 The biological functions of IFN-α/β and IFN-γ are dependent on the phosphorylation of Stat 1,...

Ngày tải lên: 20/06/2014, 04:20

... shown that a small portion of cancer cells known as "cancer stem cells" or "cancer stem- like cells" are responsible for the antagonism of the disease, resistance to therapy, self-renewal and unlimited ... inhibition of NF-κB compared to the treatment of cells with any of the drugs alone (Figure 4) These results suggest that the pretreatment of cells with genistein sensitized ovarian cancer cells, ... ovarian cancer cells [20]; however the role of genistein in chemo-resistant ovarian cancer cells has not been investigated Therefore, the intent of this study was to evaluate the effect of genistein...

Ngày tải lên: 20/06/2014, 07:20

... future study, the knockdown study of Bcl-xL using siRNA may further confirm their role of taxol-treated DCs Although the expression of Bax was increased in TaxolDCs, the expression of Bax occurred ... increased the expression of the anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit molecules into the DC nucleus Furthermore, we found that the inhibition of NF-κB by ... rationale for the use of taxol in DC immunotherapy and insights into the development of combinational DC therapies with other anticancer drugs that not harm the viability or activity of DCs Acknowledgments...

Ngày tải lên: 07/08/2014, 23:22

... γ-counter Thereafter, the total radioactivity recovered per animal was calculated by adding the counts of the organs and the rest of the body (a) The proportion of radioactivity found in the isolated ... AIA is dominated by cells of the innate immune system [19], and the exacerbation of arthritis in CD25-depleted mice could be due to a lack of suppression of these cells by Treg cells In accordance ... the disease chronic [16,17] Beyond the 100% incidence of arthritis, another major advantage of the AIA model is that the time point of induction of arthritis is known, allowing manipulation of...

Ngày tải lên: 09/08/2014, 06:22

... day and detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently ... over half of the cells in the colony expressed N-cadherin Scale bar, 10 μm in B was sufficient to promote the formation of neuroblast cells derived from ES cells We next tested the effects of different ... provided the mES cells and participated in the design of the study CSL, IMC SZL and HLS participated in the design of the study and performed the statistical analysis All authors read and approved the...

Ngày tải lên: 10/08/2014, 05:21

... (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle -like cells Unlike the round-polygonal cells, HPB-AML-I cells with the spindle -like morphology ... consisted of cells with a flattened or spindle -like morphology and that the capability of differentiating toward adipocytes of the spindle -like MSCs was superior than that of the flattened cells ... Phenotypic profiles of HPB-AML-I The expression of MSC-related antigens in the HPB-AML-I cell line is shown (A) CD45 expression of round-polygonal HPB-AML-I cells (upper) and of the cells, which...

Ngày tải lên: 10/08/2014, 10:20

... only of 6, and of inoculations of the same number of the non-SP cells grew tumors, and of 6, and of inoculations of the same number of MCF-7 cells grew tumors The tumors derived from non-SP cells ... the activation of the Wnt pathway promotes the proliferation and selfrenewal of SP cells, and the DDP only inhibits non-SP cells (differentiated cells) leading to the survival of cancerstem like ... effects of chemotherapy and radiotherapy by improving the quality of life, regulating the immune function of cancer patients and synergizes the therapeutic effects of chemotherapy and radiotherapy...

Ngày tải lên: 10/08/2014, 10:21

... Sall4B expanded cells, × 10 15 WT -like B6.SJL Sall4B expanded cells, × 106 11 WT -like B6.SJL GFP-infected cells, × 106 15 WT -like 11 WT -like B6.SJL Number of injected cells GFP-infected cells, × 10 ... LSK cells and three mice received Sall4B treated LSK cells (a) The population of donor cells (Ly5.2+) in the peripheral blood of the recipient mice was examined by flow cytometry assays with the ... removed by washing cells times, then G-CSF (R&D Systems, Minneapolis, MN) was added to cells at a final concentration of 200 ng/ml CFU assay of BM cells Tubes of MethoCult® GF M3434 (Stem Cell Technologies,...

Ngày tải lên: 10/08/2014, 21:23

... numbers of NK cells have been reported in patients with BD [49] The down-regulation of Qa-2 by siRNA might increase the number of NK cells, and the increase of NK cells might play an important role ... to play roles in the immunopathogenesis of Behcet's disease [46,47] Functionally, HLA-G directly inhibits the cytolytic function of peripheral blood NK cells [48] The frequency of NK cells was ... constructed in PC3.1 vector and then administered to normal and BD mice Cloning of the Qa- To confirm the relationship between HLA-G and the NK cells, the frequency of NK cells was observed in BD and...

Ngày tải lên: 11/08/2014, 03:20

... including the cytoplasmic NLRs and RLRs play important roles in the induction of lung inflammation For example, the cytoplasmic NALP3 protein, a member of the NLR family that triggers assembly of the ... [207-211] During RSV infection, the viral G protein mediates attachment to lung epithelial cells and the F Page of 14 protein leads to the fusion of the viral envelope with the host cell plasma membrane ... secretion in the A549 alveolar epithelial cells [143] Taken together, these findings point to dynamic regulation of airway epithelial and AM TLRs in response to diverse environmental stimuli The differences...

Ngày tải lên: 11/08/2014, 03:20

... manifestation of the reperfusion injury In order to determine the usefulness of therapies against mast cells, it is important to know the degree to which mast cells are involved in IR injury of other ... muscles to IR injury irrespective of the other abnormalities in these mice The role of mast cells has not been considered when investigating the susceptibility of different muscle fibre types Figure ... depleted mice are the desirable model to study the effects of IR on muscle fibre type Alternatively, the soleus is the most suitable muscle to study the role of mast cells since it has the least fibre...

Ngày tải lên: 11/08/2014, 08:21