the ovary cell death in mammals

... 269) investigate the mechanism behind the DENSPM-induced cell death in the L56Br-C1 cells with the further aim of identifying the markers for an apoptotic response to polyamine depletion The L56Br-C1 ... analysis The cells were sonicated in 50 mM Tris/HCl (pH 7.5) containing 0.25 M sucrose The activity of SSAT in the sonicate was determined by measuring the synthesis of [14C]acetylspermidine after incubation ... melanoma cell line that contains a mutated p53 gene In the present work, DENSPM was also found to rapidly and extensively induce cell death in the human breast cancer cell line L56Br-C1 This cell line...

Ngày tải lên: 17/03/2014, 23:20

... on cell viability might be a result of the onset of a programmed cell death process, we analyzed the effect of siRNA in the presence of the protein synthesis inhibitor puromycin As shown in Fig ... puromycin significantly reduced the ANT1 siRNA-mediated effect on ADF cell viability, indicating that the reduction in cell viability induced by ANT1 siRNA requires protein synthesis ANT1-silencing induces ... to propidium iodide staining (data not shown), indicating that the plasma membrane is maintained intact, allowing us to exclude primary necrosis as the principal cell death modality Moreover,...

Ngày tải lên: 29/03/2014, 09:20

... to the release of factors that initiate another cycle of oxidative and inflammatory insults The positive feed back loop may continue until the additional neuronal death (~50% of the remaining ... dopaminergic system against MPTP-induced neurotoxicity and behavioral deficits This study was conducted in attempt to fill in the gap within the literature on the effects of COX-2 inhibition in ... chart depicting the role of the vicious circle in dopaminergic neurotoxicity For this study, the initial insult MPP+ exerts direct dopaminergic neurodegeneration (~30% of the original numbers)...

Ngày tải lên: 19/06/2014, 22:20

... death induction, or both Therefore, in a next step, we examined whether the anti-neoplastic effects of ErPC3 and ionizing radiation include induction of cell death, in particular apoptosis These investigations ... reducing the number of viable cells in the WST-1 assay, ionizing radiation did not induce significant apoptotic nuclear fragmentation in LNCaP cells (Figure 2D) In line with these findings, caspase-3 ... that the Akt-inhibitor ErPC3 increases radiation-induced apoptosis in prostate cancer cells The most prominent combination effects were obtained in LNCaP cells that did not show any apoptosis in...

Ngày tải lên: 09/08/2014, 09:20

... in the existing air volume in the airways so that the resulting toxicity is not instantly detrimental for the epithelium, or the tissues surrounding it Instead, chronic smoking results in the ... At the end of the incubation period, cells were collected, washed in cold PBS and stained with Annexin V FITC/PI, according to the manufacturer's instructions Untreated cells were also stained, ... (400 g) The cells were resuspended at × 106/ml in pre-warmed JC-1 working buffer containing M JC-1 and incubated for 15 in a 37°C/5% CO2 incubator Subsequently, the cells were washed in assay...

Ngày tải lên: 12/08/2014, 14:20

... response and cell death in Blastocystis Microbiology 156: 665-677 Binhui Wu*, Jing Yin*, Catherine Texier, Michael Roussel, and Kevin SW Tan (2010) Blastocystis legumain is localized on the cell surface ... Slovenia Jing Yin and Kevin S W Tan Staurosporine-induced programmed cell death in Blastocystis In 4th International Conference on Anaerobic Protists, 12 – 16 May 2008, Taoyuan, Taiwan Binhui Wu, Jing ... abolished by the MOMP inhibitor cyclosporin A and significantly inhibited by the broad cysteine protease inhibitor iodoacetamide Interestingly, the apoptosis phenotype was insensitive to inhibitors...

Ngày tải lên: 16/10/2015, 15:35

... especially mitochondrial cell death, remains unclear In the present study, we investigated the basis for interaction between the signaling pathway and the celldeath machinery in response to radiation ... determine the percentage of cells within a population that are actively undergoing apoptotic cell death For the cell- death assessment, the cells were plated in 60 mm dish at a density of · 105cells ... protein kinases (MAPKs) have been implicated in the regulation of apoptotic cell death in response to various stimuli To investigate a potential involvement of MAPK in ionizing radiationinduced cell...

Ngày tải lên: 07/03/2014, 05:20

... imatinib therapy have point mutations in the BCR–ABL kinase domain, including the T315I gatekeeper mutation that impairs imatinib binding [58,59] Accordingly, new therapies are being tested and these ... drug binding) but may also arise as a result of the amplification of other oncogenes Gefitinib- or erlotinib-induced NSCLC cell death proceeds via the cell- intrinsic mitochondrial pathway, and increased ... [56,57] The importance of the BCR–ABL tyrosine kinase in the survival of CML cells led to the development of the tyrosine kinase inhibitor imatinib (STI571, Gleevec), which is a potent inhibitor...

Ngày tải lên: 16/03/2014, 00:20

... eliminate intracranial cancer cells, by either inducing cell death or preventing further proliferation; b) to explore the molecular mechanism of cell death by Ruta + Ca3(PO4)2 treatment of brain ... selectively in the cancer cells and by inducing cell proliferation in the normal cells Although these cancer cells showed different degrees of sensitivity to Ruta treatment in vitro, the bulk of the ... aberrations in the cells receiving the combination treatment indicated a protection from H2O2 insults to B-lymphoid cells by Ruta treatment (Table II) These results indicate that instead of inducing...

Ngày tải lên: 22/03/2014, 17:20

... endocytosis; the translocation domain mediates the processing of the fusion protein in the endosome or lysosome by furin to release into the cyotsol a C-terminal moiety; the released moiety containing the ... estrogen within the ligand-binding domain (LBD) of the ER complex In contrast, the three-dimensional triphenylethylene binding in the LBD cavity prevents full ER’s activation by keeping the LBD open ... encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of estrogen, the...

Ngày tải lên: 23/03/2014, 17:20

... galactose, as indicated, and in the presence of doxycyclin Cells were then washed and transferred in the same medium in the absence of doxycyclin Catalase activity was measured in cells extracts ... dioctylphtalate on h-Bax-induced cell death kinetics WtB1 cells were grown in a commercial Yeast Nitrogen Base medium (Difco, h), or in a home-made minimal medium in the absence (j) or in the presence (.) ... and in the presence of doxycyclin (Bax-repression) At t ¼ 0, cells were washed and transferred in the same media in the absence of doxycyclin (Bax-expression) The number of colony-forming cells...

Ngày tải lên: 23/03/2014, 21:20

... resuspended in the binding buffer (500 μl) FITC-Annexin-V (5 μl) was added to the cells followed by addition of μl PI according to the protocol of the Annexin V-FITC/PI kit (EMD Biosciences) The samples ... concentration in NB4-R2 cells (Figure 2C), indicating ATRA failed to inhibit NB4-R2 cells growth VX-680 decreases pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied the ... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining...

Ngày tải lên: 18/06/2014, 19:20

... resuspended in the binding buffer (500 μl) FITC-Annexin-V (5 μl) was added to the cells followed by addition of μl PI according to the protocol of the Annexin V-FITC/PI kit (EMD Biosciences) The samples ... concentration in NB4-R2 cells (Figure 2C), indicating ATRA failed to inhibit NB4-R2 cells growth VX-680 decreases pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied the ... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining...

Ngày tải lên: 20/06/2014, 03:20

... endocytosis; the translocation domain mediates the processing of the fusion protein in the endosome or lysosome by furin to release into the cyotsol a C-terminal moiety; the released moiety containing the ... estrogen within the ligand-binding domain (LBD) of the ER complex In contrast, the three-dimensional triphenylethylene binding in the LBD cavity prevents full ER’s activation by keeping the LBD open ... encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of estrogen, the...

Ngày tải lên: 27/06/2014, 11:20

... [32] Disc cells were certainly much more effective at ingesting the beads than the fibroblast cell line, L929 Cells in other cartilaginous tissue are also reported to be capable of becoming phagocytic; ... disc cells to phagocytose and the capability of apoptopic disc cells to stimulate phagocytosis could be beneficial to the intervertebral disc in vivo in many ways Death of the cells within the intervertebral ... fluorescent cell linker mini kit (catalogue number MINI-26; Sigma-Aldrich) NP or HeLa cells (1 × 106) in mL/well were tagged Apoptosis was induced in the NP and HeLa cells by exposing them to UV...

Ngày tải lên: 09/08/2014, 10:23

... maintains chromatin structure to ensure the passage through the S-phase checkpoint [44], are expressed in an anti-correlated manner, peaking in the G1/S and G2/M transitions, respectively The ... transcripts Most of the proteincoding messages opposite the 37 periodic antisense transcripts (13 + 24) fall into GO categories linked with the process of cell division, including cell wall and organelle ... annotated coding genes are indicated by blue boxes The heatmap in the upper half of each panel represents signal on the Watson strand, the one in the lower half signal on the Crick strand The horizontal...

Ngày tải lên: 09/08/2014, 20:21

... fiction? The issue of determining death becomes further confused by the Uniform Determination of Death Act (UDDA), which, ironically, was drafted with the intent to clarify the issue The UDDA guidelines ... with these issues if we hope to differentiate the dead from the imminently dying Competing interests The authors declare that they have no competing interests References Whetstine L: An examination ... Gert B: On the definition and criteria of death Ann Intern Med 1981, 94:389-394 Guidelines for the determination of death Report of the medical consultants on the diagnosis of death to the President’s...

Ngày tải lên: 13/08/2014, 03:20

... of the immune system in combating the infection and thus determine the survival of the patient It is known that cellular immune responses play a critical role in the defense against viral infections ... , and the findings of other investigations have supported the notion that T lymphocytes are involved in the pathogenesis of septic shock [8-12] In this translational study, we have further characterised ... PBMC were incubated with combinations of fluorescein isothiocyanate (FITC), phycoerythrin (PE), phycoerythrin-cyanine 5.5 (PE-cy5.5), peridinin chlorophyll protein (PerCP) and allophycocyanin (APC)-labelled...

Ngày tải lên: 13/08/2014, 15:21

... response Furthermore, apoptosis of gastrointestinal epithelial cells may compromise the integrity of the bowel wall, resulting in the translocation of bacteria or endotoxins into the systemic ... Nevertheless, these promising findings must be tempered by the new study showing that multiple pathways might be activated in dying cells during sepsis [1] The simple inhibition of a particular cell ... Another important distinguishing feature of apoptotic versus necrotic cell death relates to inflammation When the necrotic cell ruptures, an intense inflammatory response follows, owing to the...

Ngày tải lên: 13/08/2014, 18:22

... the early signaling of apoptotic cell death in virus-infected animal cells [29] In plant cells, serine proteases have been implicated in pathogen-induced cell death [35,36], and serine protease ... the corresponding protein participates in a process that is important for the cell or tissue We searched for highly abundant transcripts in the process of fiber -cell death by identifying in the ... of the cell walls, but their function has remained unclear A recent report on a hybrid protein containing AGP domains supports the hypothesis that AGPs have a critical function in mediating cell- cell...

Ngày tải lên: 14/08/2014, 14:21