the molecular diagnosis of sexually transmitted genital ulcer disease

... to Chronic HBV Disease When hepatitis B virus (HBV) infection is suspected, it is the appearance of serum markers of HBV infection that establishes the diagnosis of the disease Of these, HBsAg ... technology, molecular beacons) that decrease the number of handling steps, reduce contamination, and increase throughput and the accuracy of quantification will further enhance the utility of these ... exposure to the virus until the appearance of telltale symptoms HBsAg is also the key marker in determining whether hepatitis B infection has become chronic: defined as the persistence of the surface...

Ngày tải lên: 03/11/2012, 09:41

... in the one-cell stage to visualize the localization and to measure the mobility of the components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of ... of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized in the ... outside of the nuage in the cytoplasm of the migrating PGC FCS analysis revealed that Olvas GFP diffused with a diffusion constant of 7.0 lm2ặs)1 (Fig 3A) During the measurement of the PGC, the 10...

Ngày tải lên: 18/02/2014, 16:20

... (pTDN1) [14] The lack of characterization of the structural determinant of the substrate specificity of the AtdA enzyme has thus limited its development as a biocatalyst for the bioremediation of a wide ... reported which of the five genes control the substrate specificity of the AtdA enzyme The objective of this study was to identify and probe the residues determining the activity as well as the substrate ... may indicate that the steric limitation of the enzyme’s binding pocket takes place in the area between the ortho and para positions of the aromatic substrate On the basis of these results, aniline...

Ngày tải lên: 19/02/2014, 02:20

... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability of the structure of the protein The difference of the increments ... surrounding residues of the depression, and the side chain of the mutated residue might restrain the mobility of the N-terminal region on the surface by interaction with the main chain of Lys26 by van ... the substitutions did not influence the activities or specificity of the mutant enzymes The N-terminal region of ALP I was also on the surface The side chains of Ile10 and Tyr11 were close to the...

Ngày tải lên: 21/02/2014, 03:20

... the location of amyloidogenic mutations and position of b-strands (A) The structure of the polypeptide backbone of the two chains (purple and blue) is shown along with the location of the N-terminus ... these diseases, in particular Alzheimer’s disease, may help to explain some aspects of the pathogenesis of FAP The idea is discussed further in the following sections Tissue-specific pattern of ... tissue and that the distribution of TTR deposition reflects the presence of these endogenous factors In the case of the Ab protein of Alzheimer’s disease, a number of proteins and factors (pathological...

Ngày tải lên: 07/03/2014, 10:20

... loss, within affected cells, of the two alleles of a given CCM gene Loss of one of the alleles (first hit) would be the result of a germline mutation and loss of the second allele (second hit) ... clinical care of the patients and were an important step towards the understanding of the mechanisms of this disorder This minireview summarizes the advances in CCM molecular genetics and the remaining ... K569E, were detected in the CCM lesion and were shown to be absent in the blood of the patient However, the data were difficult to interprete because of the nature of the mutations which were...

Ngày tải lên: 15/03/2014, 10:20

... earlier, the molecular identity of the protein(s) that actually form the PTP channel remains the most intriguing question On the basis of structural and other information, we hypothesize that the ... increased the conductance of artificial lipid bilayers in the presence of Ca2+, and the conductance was sensitive to ruthenium red [22], implying that it may be the Ca2+ uniporter or part thereof Further ... is driven by the pH gradient, in the case of the Ca2+ ⁄ 2H+ exchanger Elevated intramitochondrial Ca2+ can stimulate the activities of enzymes of the tricarboxylic acid cycle (TCA), thereby boosting...

Ngày tải lên: 23/03/2014, 03:20

... Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications high grade gliomas The structure and compounds of the ECM of the brain tissue have many specific differences from other ... mortality of the patient Moreover, the presence of the blood-brain barrier (BBB) limits the passage of a wide variety of anticancer agents The high incidence of recur‐ rence and poor prognosis of malignant ... Hofmann M Characterisation of the murine gene encoding the intracellular hyaluronan receptor IHABP (RHAMM) Gene 1999;226:41 23 24 Evolution of the Molecular Biology of Brain Tumors and the Therapeutic...

Ngày tải lên: 23/03/2014, 10:20

... characterized the contribution of these assay components by omitting them individually In the absence of ferredoxin the reaction rate decreased to 46.5% of the complete reaction while the absence of ascorbate ... Arrows indicate the direction of the major changes in absorption over the course of the measurements (C) Michaelis– Menten plot of the PsHO1 reaction for heme concentrations of 1, 2, 5, 10 and ... account for the failure of the PsHO1 probe to detect any HO2-like sequences in Southern blot experiments Whatever the basis of the recovery of phytochrome responses in older pcd1 plants, they still...

Ngày tải lên: 30/03/2014, 11:20

... on the basis of the P value of 0.005 (ii) What is the probability of obtaining a correlation coefficient so far from zero for at least of the cancer cell types in the panel if the null hypothesis ... http://genetics.nature.com of the 118 drugs We then clustered the 118 drugs on the basis of tered paradoxically with the alkylating agents, perhaps implying that drug metabolism rather than mechanism of action is an these ... mechanisms of action based on their activity patterns across the 60 cell lines b, Cluster tree of the 118 drugs based on the correlation of their activity patterns with expression patterns of the genes...

Ngày tải lên: 30/03/2014, 13:20

... provides a good yield of product, and is excellent regardless of the quality of the DNA template From: Methods in Molecular Medicine, vol 92: Molecular Diagnosis of Genetic Diseases, Second Edition ... according to size with the WAVE buffer system (Fig 2A) Essentially, the DNA interacts electrostatically with the ammonium ion of the TEAA on the basis of size The larger the DNA, the more interactions ... surface of the DNASep matrix Elution of DNA from the DNASep matrix occurs through the use of the hydrophobic eluant ACN, which reduces the hydrophobic interaction between the stationary phase and the...

Ngày tải lên: 11/04/2014, 09:51

... Role of the Professional Bodies In the United Kingdom, a number of professional bodies have had an interest in the development of clinical molecular genetics over the last 10 yr Of note are the ... in the migration of the single-stranded DNA is detected (see Fig 2), then the rest of the family of the individual concerned should be analyzed to exclude the presence of a polymorphism rather ... encompass the whole of the region complementary to the cloned DNA in the cosmtd, then the labeled cosmid will hybridize to the deleted chromosome and the test becomes invalid Therefore, if the cosmid...

Ngày tải lên: 11/04/2014, 09:52

... preparative IEF of proteins with the Rotofor unit, the voltage will gradually increase during the course of the run and then plateau (generally around 1200–1400 volts) The voltage of the Rotofor unit ... resulting double-stranded molecules The molar ratio of abundant to less abundant sequences in the product will then be of the order of the square of the initial ratio of the concentrations, which eventually ... spot the columns in the small boxes correspond to the intensity in each immunoblot of the analysis set The numbers in the upper right corners of the small boxes show the maximum intensity of each...

Ngày tải lên: 11/04/2014, 09:52

... characterization of viruses related to the SARS coronavirus from animals in southern China Science 2003, 302:276-278 Chinese SMEC: Molecular evolution of the SARS coronavirus during the course of the SARS ... late in the epidemic, a slowing of missense mutations is seen over time, with the most extensive changes occurring in the S protein during the early stages of the outbreak [18] This suggests the ... administration of corticosteroids [44,45] Since then, the wealth of information that has emerged on SARS-CoV molecular and cellular biology, as updated in the preceding sections of this review, now offers...

Ngày tải lên: 19/06/2014, 08:20

... [14] The rapidity of the technique without any compromise on sensitivity has made SVC very popular in the field of clinical virology The same technique has been employed for the identification of ... *Four out of fifty patients included in the study left the hospital against medical advice, the clinical outcome of whom is not known Hence, these cases were excluded from the analysis of clinical ... Among the 50 patients included in the study, (8%) patients left against medical advice, so the clinical outcome of these cases is not known Out of the remaining 46 (92%), 16(34.8%) expired and the...

Ngày tải lên: 19/06/2014, 08:20

... characterization of viruses related to the SARS coronavirus from animals in southern China Science 2003, 302:276-278 Chinese SMEC: Molecular evolution of the SARS coronavirus during the course of the SARS ... late in the epidemic, a slowing of missense mutations is seen over time, with the most extensive changes occurring in the S protein during the early stages of the outbreak [18] This suggests the ... administration of corticosteroids [44,45] Since then, the wealth of information that has emerged on SARS-CoV molecular and cellular biology, as updated in the preceding sections of this review, now offers...

Ngày tải lên: 20/06/2014, 04:20

... [14] The rapidity of the technique without any compromise on sensitivity has made SVC very popular in the field of clinical virology The same technique has been employed for the identification of ... *Four out of fifty patients included in the study left the hospital against medical advice, the clinical outcome of whom is not known Hence, these cases were excluded from the analysis of clinical ... Among the 50 patients included in the study, (8%) patients left against medical advice, so the clinical outcome of these cases is not known Out of the remaining 46 (92%), 16(34.8%) expired and the...

Ngày tải lên: 20/06/2014, 04:20

... by counting the number of alleles per locus and taking the average of these counts These values then can be compared with the effective number of alleles (ne), which is the number of alleles needed ... etc Another impact of the Pleistocene glacials pertained to the connection between the Borneo and Thai-Malay Peninsula (the continent of Asia) The land bridge of the Sundaland accelerated the migration ... used at the level of the individual, and produces distances generated from the bands rather than taking into account the diversity of the population The disadvantage is that it treats the data...

Ngày tải lên: 28/06/2014, 11:20

... Tsix The role of DNA methylation Figure Transcription of Xist and Tsix on the X chromosome The coding sequences of Xist and Tsix overlap on opposite strands of the X-chromosome DNA effect: the ... initiated A second cause of non-random X-chromosome inactivation is the selective death of cells that inactivate the incorrect number of X chromosomes: because the fates of the X chromosomes are ... transcriptional silencing [15] These epigenetic marks also characterize the XI, and their recruitment to the XI requires transcription of Xist [16-18] Together, these results suggest that the increase in Xist...

Ngày tải lên: 06/08/2014, 18:21

Ngày tải lên: 07/08/2014, 17:22