the material composition and structural design of bone

... both motions of the pair The vector of the response spectra is calculated as the square root of the sum of the squares (SSRS) of the response spectra for the two different directions, and this vector ... representative of the overall loaddeformation behavior of the building and compute a response history using appropriate software (e.g., Hachem, 2004) Dynamic analysis of the oscillator under the design ... While the importance of deformation is paramount in performance-based assessment and design of yielding structures, the design should not overlook aspects of performance that may be affected by other...

Ngày tải lên: 21/07/2014, 20:20

... at their 50 and 30 boundaries, respectively, and were inserted between the third letter of one codon and the first letter of the following codon Molecular modeling of the C sepium b-amylase The ... the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the allowed regions of low energy, except ... Cys97, Cys208 and Cys343) On the analogy of the soybean b-amylase, the active site of the C sepium b-amylase most probably consists of a cleft located between the barrel core and the smaller globular...

Ngày tải lên: 22/02/2014, 07:20

... sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The ... sequences of the peptides, the position in the complete sequence of Cyn d 24 is indicated the amino acids of number and number peptide sequences (Fig 2B), and an antisense primer, designed on the basis ... to the N-terminal portion of the allergen Based on this partial sequence, two specific primers were designed and used together with the anchor primer AP2 to obtain the 5¢ and 3¢ portions of the...

Ngày tải lên: 07/03/2014, 12:20

... indicated by the presence of a very strong negative band at 198 nm The negative band near 220 nm results from overlapping of the bands of b-sheet (215 nm) and a-helix Table Fractional composition of secondary ... component bands, determined by band fitting of the absorbance spectrum of Fig 4A, are shown in Fig and Table From Table 2, the sum of individual amide I¢ intensity and the intensity percent of each ... studies of the catalytic subunit of the cAMP-dependent protein kinase Its ability to readily alter the PKI coding sequence could permit further studying of human PKIb and understanding of the function...

Ngày tải lên: 07/03/2014, 15:20

... on the effect of two variables, the reaction time and the presence of chloride ions, on the stoichiometry, stereochemistry and degree of folding of the Hg(II)-MT species formed by either the ... of all the variables in the Hg-MT system required the performance and analysis of 18 Ó FEBS 2004 titrations and the corresponding duplicates The detailed and comparative analysis of the set of ... Notwithstanding this, there is a minor effect of t and X at the beginning and end of the titration Thus, the addition of the first 4–6 of Hg(II) to apo-MT gives rise to Hg-MT species of comparable...

Ngày tải lên: 16/03/2014, 18:20

... 3, and the 5¢ part of exon encode the mature protein; exon encodes the 5¢-UTR, and exon and part of exon encode the signal peptide The 110 nucleotides at the 3¢ end of exon encode the 3¢-UTR The ... Ib in the genome of this snake These findings provide clues to the evolutionary position of the neurotoxic V a aspis population with respect to the other snakes studied The genome of one of these ... indeed the case, then substitutions occurring in these areas may affect the neurotoxicity or anticoagulant effect of the PLA2 The substitutions in the A and B chains of vaspin, in the inhibitor and...

Ngày tải lên: 17/03/2014, 03:20

... cloning of the gene encoding the secreted CM from Yersinia pestis (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the ... form, and the rest of the structure is in cartoon form In EcCM, one polypeptide chain is gray and the other is rose In 90-MtCM, one polypeptide chain is blue and the other is green The TSA from the ... performing the role of Gln88 in EcCM Of the other two structures, PfCM has the conserved Ser70 and Gln74, and TtCM has Ser81 and Glu85 There are two Protein Data Bank files for TtCM; in the file 2D8D,...

Ngày tải lên: 17/03/2014, 17:20

... with the native state of the protein We then carried out the characterization of the thermal properties of the mutant in comparison with those of PfPNP The results obtained indicate that the substitution ... oxidation-reduction to rescue the possible damage of the other two disulfide bonds The presence of a low percentage of the protein with Cys254 and Cys256 in the reduced form further supports this hypothesis It has ... arrangement of PfPNP The presence of three disulfide bonds justify the extreme stability features of PfPNP These covalent links, in fact, lowering the entropy of the unfolded polypeptide and introducing...

Ngày tải lên: 23/03/2014, 09:21

... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate ... about the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) of the Ndh complex To contribute to the structural and functional characterization of this ... characterize the Ndh complex, the MS and BS chloroplasts were separated on CN ⁄ PAGE (1D, based on the internal charge of the protein complexes) and BN ⁄ PAGE (2D, based on the external charge of the...

Ngày tải lên: 23/03/2014, 13:20

... excitation of H-1 H-resonance of the GlcNAc residue in the TOCSY step and of H-3/H-4 1H-resonance of the GlcNAc residue in the NOESY step (A) The assignments of the 1H-resonances of the Gal II ... unit is linked to the Hep I residue of the LPS inner core in the sialylated glycoform The structure of the sialylated glycoforms of the O-deacylated LPS of H influenzae strain RM118 and mutants causing ... shown) and establishing the structure of the tetrasaccharide to be, a-Neu5Ac-(2–3)-b-D-Gal-(1–4)-b-D-GlcNAc-(1–3)-b-DGal Due to the low intensity of the sialylated glycoforms and a high degree of...

Ngày tải lên: 23/03/2014, 21:21

... (located at the C-terminus of the a2-helix) and Ala112 (located at the C-terminus of the b7-strand connected with the base of the b5 ⁄ b6 loop by hydrogen bonds) The conformational change of Ala112 ... the loss of the interaction of the a2-helix with membrane interface) is one of the factors that destabilize the membrane binding state of the PH domain As shown in Table and Fig 2, addition of ... for the PH domain and consequently for PLC-d1 Dynamic changes in the lipid composition of the plasma membrane would be capable of providing a means of regulating the properties of PLC-d1 and the...

Ngày tải lên: 30/03/2014, 10:20

... K8 and D72 are located near the crossing region of the helices, and the Nx atom of K8 is found ˚ at a distance of 6.2 A from the nearest side-chain oxygen of D72 Taking the functionally and structurally ... neutralization of the effect of the mutation D72K on the protein’s global charge was not sufficient to fully reverse the negative effect on the stability of cyt c6 The apparent stability of the mutants ... along the C-terminal a helix to E68 and Y76 Interestingly, one of the side chain oxygens of D72 appears to be hydrogen-bonded to the backbone N atom of G6, bridging the crossing between the N- and...

Ngày tải lên: 30/03/2014, 20:20

... interfering with the reaction between the endotoxins and the LAL As a consequence, the suitability of the regents and materials used and the product itself has to be established The endotoxin limit ... of micro-organisms is obtained after incubation in presence of blood and the ATMP, the product possesses no antimicrobial activity under the conditions of the test, and the sterility may be then ... and coordination All the authors read and approved the final manuscript References Regulation (EC) No 1394/2007 of the European Parliament and of the Council of 13 November 2007 on advanced therapy...

Ngày tải lên: 18/06/2014, 15:20

... due to the geographical location and the harvesting period It is noteworthy that the results of this study may be considered as the first report on the composition of the essential oils of this ... order of their elution on the HP-5MS non-polar column (Table 1) A total of 19 compounds were identified, representing 86.68% of the total oil The esters made up the largest component of the oil ... [4] and the neuropharmacological properties [5] have been investigated as well To the best of the authors’ knowledge, there are no reports about the chemical content and biological effect of the...

Ngày tải lên: 20/06/2014, 20:20

... due to the geographical location and the harvesting period It is noteworthy that the results of this study may be considered as the first report on the composition of the essential oils of this ... order of their elution on the HP-5MS non-polar column (Table 1) A total of 19 compounds were identified, representing 86.68% of the total oil The esters made up the largest component of the oil ... [4] and the neuropharmacological properties [5] have been investigated as well To the best of the authors’ knowledge, there are no reports about the chemical content and biological effect of the...

Ngày tải lên: 21/06/2014, 17:20

... noteworthy that the results of this study may be considered as the first report on the composition of the essential oils of this endemic species Chahma A voucher specimen was deposited at the herbarium ... was found between the oil content of L resedifolia and that of L arboresens as was reported by Cheriti et al [6] The slight difference may be due to the geographical location and the harvesting ... reported in the literature [12] and stored on the MS library (NIST database) The concentration of the identified compounds was computed from the GC peak total area without any correction factor The compounds...

Ngày tải lên: 21/06/2014, 19:20

... for at 70 ◦ C, then mixed with a 5× first-strand buffer, 10 mM dNTPs, and 0.1 M DTT The reaction mixture was further incubated for at 37 ◦ C, then for 60 after the addition of U of MuLV reverse ... fractions The yield of the neutral fraction (CPSN) and the acidic fraction (CPSA) obtained from the crude polysaccharide extract CPS was 0.328 g/g and 0.034 g/g, respectively (Fig 1A) The molecular ... analysis of CPSN Fr II and standard polymers according to the absorption maximum of the congo red-polysaccharide complex at various concentrations of NaOH For more details, see Section (C–H), and...

Ngày tải lên: 28/06/2014, 11:20

... limitant, and the sand-silty and acid soil favour chestnut growth in the Park, according to the results of Bernetti [5], Leonardi et al [34] and Rubio and Escudero [54] The higher SOM and Influence of ... persistence of other problems associated to this perturbation, mainly leaching The correlation analysis (Tab III) underlined the dependence of LAI on the considered structural traits of the stands In ... stool and the invigorating effects of decapitation which could cause an initially fast growing of the coppice sprouts, according to the results of Kauppi and Kiviniitty [30] and Rinne et al [52] These...

Ngày tải lên: 08/08/2014, 00:22

... drafting of the manuscript MA contributed with plant material, the general idea of the study and participated in revision of the manuscript AKHE participated in the general idea of the study, the design ... to the growing of the plants, harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing the experiments, structural analysis and the ... controlling the abscission event of pea seeds Absence of the hilum palisade layers is the key characteristic in the def mutant pea seed Structural analysis revealed the absence of the palisade layer and...

Ngày tải lên: 12/08/2014, 03:20

... compromising the structural integrity of the core domain On the other hand, when the modified residues are part of the hydrophobic core of the domain, the modification could cause considerable structural ... Phosphorylation of T432 may affect the relative orientations between domains within the protein, whereas Y29 may modulate the conformation of the helical subdomain The orientations of the domains and of the ... 5% of the sites were strictly conserved across the alignments, and not more than 20% of the sites were conserved in at least 80% of all of the homologs within the alignments As expected, the...

Ngày tải lên: 14/08/2014, 07:21