the lung in systemic lupus erythematosus

... responding to immunosuppressive therapy in lupus patients should raise suspicion of alternative diagnoses such as PVNS Page of Competing interests The authors declare that they have no competing interests ... the synovial fluid effusion (white) in the dorsal recessus of the joint around the femoral head (B) Gadolinium contrast of sagittal images shows diffuse enhancement in synovial tissue along the ... orbicularis to the posterior joint cavity surrounding a contrast-free corpus librum of mm diameter (C) The synovial proliferation appears in dark grey in the T2-weighted image at the same location...

Ngày tải lên: 10/08/2014, 23:20

... mutations within the CDRs, of which the most prevalent was from serine to asparagine; other mutations included serine to threonine, tyrosine, aspartate, methionine and proline Discussion The immunohistology ... B-cell cluster D The arrows indicate the areas of positive staining for the marker in question All images: 100× In total, 15 rearranged VH sequences were analyzed (including seven independent V-D-J ... cells The aims of the present study were to identify the immunoglobulin V genes used by proliferating B cell clones in GCs of a SLE spleen, and to determine whether there are abnormalities in the...

Ngày tải lên: 09/08/2014, 01:21

... Takeda T, Inoue T: Serum levels of interferons in patients with systemic lupus erythematosus Clin Exp Immunol 1987, 70:562-569 Hooks JJ, Moutsopoulos HM, Notkins AL: Circulating interferon in human ... significant increase in the gene expressions of TNF, IFN, or IFN in themselves according to rank test, more than one-half of the SLE patients' individual data showed an increase in the TNF gene ... determined from the linear region of the amplification plot and were normalized by subtracting the Ct value of GAPDH (generating a Ct value) The response to the cytokines or E2 was determined...

Ngày tải lên: 09/08/2014, 01:22

... cardiovascular risk factors in systemic lupus erythematosus J Musculoskel Med 1998, 15: 42-52 El-Magadmi M, Ahmad Y, Wajed J, Bruce IN: The bimodal mortality pattern in systemic lupus erythematosus Curr ... abnormalities in women with SLE [abstract] Arthritis Rheum 1997, 40 (suppl):S219 Bulkley BH, Roberts WC: The heart in systemic lupus erythematosus and the changes induced in it by corticosteroid therapy ... Kong TQ, Kellum RE, Haserick JR: Clinical diagnosis of cardiac involvement in systemic lupus erythematosus: a correlation of clinical and autopsy findings in thirty patients Circulation 1962,...

Ngày tải lên: 09/08/2014, 01:22

... iodide staining, according to the method of Nicoletti and colleagues [19], and by the binding of FITCconjugated Annexin V, using the Apoptest binding kit, containing annexin V-FITC and binding buffer ... 10 at 4°C and then soaked in balanced salt solution (Sigma) for 30 at 25°C Cells were then incubated for 30 at 25°C in the blocking buffer (2% bovine serum albumin in PBS, containing 5% glycerol ... expected, the immunolabelling with a mouse polyclonal antiserum specific to the recombinant Nedd5 C-ter appeared confined to the cytoplasm, where staining of the contractile ring was evident No staining...

Ngày tải lên: 09/08/2014, 06:23

... proteins and DNA by high NaCl and possibly secondary conformational changes help the binding of anti-RPA antibodies in autoimmune sera We examined whether the binding of DNA to RPA can interfere the ... ELISA with the recombinant RPA32 protein Antibodies against RPA32 were found in 10.9% (87 of 801) in breast cancer patients and in 10.3% (4 of 39) in intraductal in situ carcinoma patients, in contrast ... autoantibodies by incubating affinity-purified RPA with ssDNA, dsDNA, or dsRNA before antibody binding When the RPA was incubated with ssDNA or dsDNA before the reaction with autoimmune sera, the binding of...

Ngày tải lên: 09/08/2014, 08:22

... antibodies in the SmD peptide ELISA [32] In the present study we confirmed the dsDNA binding property of the SmD1 peptide This finding was further supported by the inhibition of dsDNA binding of ... and in only 8.3% of controls using a SmD1 synthetic peptide [15] This peptide, but not the fulllength protein, has been shown to bind dsDNA contained in blocking reagents, which may result in the ... C, Klein R, Burmester GR, Schoessler W, Hiepe F: Casein is an essential cofactor in autoantibody reactivity directed against the C-terminal SmD1 peptide AA 83–119 in systemic lupus erythematosus...

Ngày tải lên: 09/08/2014, 10:20

... blockade in patients with systemic lupus erythematosus While dampening inflammation, TNF blockade with infliximab induced an increase of autoantibodies in five out of seven patients and increases in ... on infliximab therapy in SLE suggests that, in combination with azathioprine, infliximab may turn out to be an interesting therapeutic option in selected patients with SLE and in particular in ... However, in view of the autoimmunogenic potential of inhibiting TNF, we tested TNF inhibition as a means to interfere with the inflammatory response in SLE by administering only an induction...

Ngày tải lên: 09/08/2014, 10:22

... located in the introns of STAT4 and in LD with each other In contrast, significant association was not detected for SNPs in the STAT1 region (P > 0.05) To confirm the association detected in the ... of systemic lupus erythematosus Arthritis Rheum 1997, 40:1725 23 Schildkraut JM: Examining complex genetic interactions In Approach to Gene Mapping in Complex Human Diseases Edited by: Haines ... genotyping and statistical analyses, and wrote the manuscript II, KH, MK, and TA participated in the first screening using Illumina GoldenGate assay (with AK), including tag SNP selection, genotyping,...

Ngày tải lên: 09/08/2014, 13:22

... Takeda T, Inoue T: Serum levels of interferons in patients with systemic lupus erythematosus Clin Exp Immunol 1987, 70:562-569 Hooks JJ, Moutsopoulos HM, Notkins AL: Circulating interferon in human ... significant increase in the gene expressions of TNF, IFN, or IFN in themselves according to rank test, more than one-half of the SLE patients' individual data showed an increase in the TNF gene ... determined from the linear region of the amplification plot and were normalized by subtracting the Ct value of GAPDH (generating a Ct value) The response to the cytokines or E2 was determined...

Ngày tải lên: 09/08/2014, 13:22

... analysed and interpreted the data and was involved in drafting the manuscript HK analysed and interpreted the data and was to some extent involved in drafting the manuscript MJ contributed to the study ... and was involved in drafting the manuscript All authors have given their final approval of the version of the manuscript to be published Competing interests The authors declare that they have ... years) The patients’ ages at the time of sampling, the time predating the onset of symptoms and diagnosis and the time after sampling until the onset of symptoms are presented in Table for both the...

Ngày tải lên: 12/08/2014, 15:22

... C-reactive protein in systemic lupus erythematosus Lupus 2009, 18:966-973 44 McMahon M, Hahn BH: Atherosclerosis and systemic lupus erythematosus - mechanistic basis of the association Curr Opin Immunol ... Other cytokines T cells The in ammatory cytokine TNF-α appears to play an important role in the initiation and perpetuation of atherosclerotic lesions in the general population It increases the ... assessment and treatment in systemic lupus erythematosus Best Practice Res Clin Rheumatol 2009, 23:481-494 83 Meyer O: Anti-CRP antibodies in systemic lupus erythematosus Joint Bone Spine 2010, 77:384-389...

Ngày tải lên: 12/08/2014, 15:22

... participated in the analysis of experimental data and in the writing of the manuscript AJ, GS, AAB, AG, BL, EL and TS participated in the design of the study, in the analysis of the patient cohorts, in the ... process in systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) even though both Gas6 and sAxl in most of the patients were within the normal range [13] Systemic lupus erythematosus ... sMer is increased in active SLE and to a lesser extent in RA In contrast, neither sMer nor Tyro3 plasma concentrations are increased in CLI suggesting that the increased sMer in SLE is not the result...

Ngày tải lên: 12/08/2014, 15:23

... Mexican Systemic Lupus Erythematosus Disease Activity Index and the Systemic Lupus International Collaborating Clinics/American Collage of Rheumatology Damage Indexes at the beginning of the study ... cytomegalovirus; SLEDAI, Systemic Lupus Erythematosus Disease Activity Index; SLICC, Systemic Lupus International Collaborating Clinics aInformation for treatment was available in 19 patients in this group ... Snaith M, Sturfelt G: The reliability of the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index in patients with systemic lupus erythematosus Arthritis...

Ngày tải lên: 09/08/2014, 01:22

... factor-α: the Janus cytokine In NZB/W F1 mice, the administration of TNF-α reduces the severity of the lupus- like illness [6] This observation has not been repeated in other lupus- prone mouse strains ... PM, Isenberg DA: The effect of interleukin-10 and interleukin-12 on the in- vitro production of antidouble-stranded DNA antibodies from patients with systemic lupus erythematosus Clin Exp Immunol ... meeting showed the breadth of interest in the role played by different cytokines in SLE A large amount of work in mice, and a smaller body of evidence on the effects of anticytokine antibodies in...

Ngày tải lên: 09/08/2014, 01:22

... cytokine interleukin-6 Biochem Pharmacol 2000, 60:1185-1195 33 Cross JT, Benton HP: The roles of interleukin-6 and interleukin-10 in B cell hyperactivity in systemic lupus erythematosus Inflamm ... DC-poietins in systemic lupus erythematosus Hum Immunol 2002, 63:1172-1180 47 Scheinecker C, Zwolfer B, Koller M, Manner G, Smolen JS: Alterations of dendritic cells in systemic lupus erythematosus: ... response; blocking TNF therefore may or may not be wise (Fig 1) In the efferent limb of the response, TNF clearly induces inflammation and local injury With respect to the local injury, blocking TNF...

Ngày tải lên: 09/08/2014, 01:22

... Symmons D, Zoma A: The development and initial validation of the Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index for systemic lupus erythematosus Arthritis ... protein autoantibody; CRP, C-reactive protein; mSLEDAI, modified systemic lupus erythematosus disease activity index; n.s., not significant; SLEDAI, systemic lupus erythematosus disease activity index ... protein (CRP) and monomeric CRP (mCRP) considering the ability to inhibit anti-CRP binding, indicating that anti-CRP autoantibodies are targeted to the monomeric form of the acute-phase protein...

Ngày tải lên: 09/08/2014, 01:23

... impairment in systemic lupus erythematosus Arthritis Rheum 1992, 35:411-416 Lindal E, Thorlacius S, Steinsson K, Stefansson JG: Psychiatric disorders among subjects with systemic lupus erythematosus in ... manifestations of systemic lupus erythematosus Aust N Z J Med 1988, 18:679-684 Johnson RT, Richardson EP: The neurological manifestations of systemic lupus erythematosus Medicine 1968, 47:337-369 Wekking EM: ... All Available online http://arthritis-research.com/content/6/4/R366 Table Demographic and clinical findings in patients with systemic lupus erythematosus grouped according to their psychiatric...

Ngày tải lên: 09/08/2014, 01:23

... final version of the manuscript DMH assisted in the design in the study, in the interpretation of the results, and in writing the final version of the manuscript DN assisted in the design in the ... of the results and in writing the final version of the manuscript TSM performed the plasma BLyS protein and anti-BLyS assays and assisted in the interpretation of the results and in writing the ... Each symbol indicates an individual subject The composite results are plotted as box plots The lines inside the boxes indicate the medians, the outer borders of the boxes indicate the 25th and...

Ngày tải lên: 09/08/2014, 07:20

... K, Nived O, et al.: The reliability of the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index in patients with systemic lupus erythematosus Arthritis ... transforming growth factor-beta Lupus 1999, 8:95-102 Bruce IN, Gladman DD, Urowitz MB: Premature atherosclerosis in systemic lupus erythematosus Rheum Dis Clin North Am 2000, 26:257-278 Grainger ... The factors in blood that modulate activation of TGF-β1 remain obscure, but the link with LDLcholesterol opens up a novel atherogenic pathway that requires further study Competing interests In...

Ngày tải lên: 09/08/2014, 08:22