the long terminal repeat of mmtv
Báo cáo y học: " 5'''' long terminal repeat (LTR)-selective methylation of latently infected HIV-1 provirus that is demethylated by reactivation signals" doc
... information on the state of the latent HIV provirus in vivo, the notion remains to be examined LTR: long terminal repeat Decipherment of the mechanisms for reactivation of latently infected HIV in the reservoir ... cells by the inverse PCR Identification of the flanking genomic sequence of the inteIdentification of the flanking genomic sequence of the integrated HIV provirus in ACH-2 cells by the inverse ... previously in studies of the HTLV-1 provirus [29] On the other hand, the response to TNF-α provides evidence of cytokine receptor signal-mediated demethylation of the provirus LTR The mechanism by...
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Báo cáo y học: " Overlapping enhancer/promoter and transcriptional termination signals in the lentiviral long terminal repeat" doc
... Therefore, the majority activity of the termination signals appear to fall within the 124 nt of U3C NFκB antibody illustrating more than 95% inhibition of expression of both p105 and p50 of the ... reduce the readthrough rate of the LV SIN LTR, three different RNA termination signals were tested: the polyadenylation signal of HTLV-1 (HTLVpA), which is located in U3 rather than R-U5 of the ... insertion of TRE reduced the readthrough rate by around 20%, regardless of the presence of rtTATS (Fig 5) In addition, induction of rtTATS binding to TRE did not influence the readthrough rate Therefore,...
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Báo cáo y học: " Kaposi''''s sarcoma associated herpes virus-encoded viral FLICE inhibitory protein activates transcription from HIV-1 Long Terminal Repeat via the classical NF-κB pathway and functionally cooperates with Tat" pptx
... analysis of the ability of K13 to activate the HIV-1 LTR and analyzed the contribution of the canonical vs alternate NF-κB signaling pathways, various subunits of the IKK complex and the HIV-1 ... regulation of HIV-1 clade-specific B, C, and E long terminal repeats by NF-kappaB and the Tat transactivator Virology 2002, 296:77-83 Blauvelt A: The role of human herpesvirus in the pathogenesis of ... [25,34-36] Along the same lines, the transactivating function of Tat protein requires the presence of NF-κB sites in the HIV-1 LTR and Tat protein is known to cooperate with NF-κB to activate the HIV-1...
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Tài liệu Báo cáo khóa học: The C-terminal domain of Escherichia coli Hfq increases the stability of the hexamer ppt
... binding of the polyadenylated rpsO RNA We have shown that the presence of the remainder of the acidic tail results in the thermodynamic stabilization of Hfq by 1.8 kcalÆmol)1 A comparison of the ... noted that the C -terminal domain of Hfq seems to be located at the same position as that of the Sm-like SmAP3 of the archaea P aerophilum [31] and, as in the case of SmAP3, the possibility of two ... protein Based on the analysis of the transition region, the conformational stability of the proteins can be calculated [29]: the free energy when Ó FEBS 2004 The role of the C -terminal domain...
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Báo cáo khoa học: The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III s subunit ppt
... test whether the removal of the second half of the PolC N-terminus would help to improve the results further Therefore, we took only the fragment of the multiple sequence alignment covering the first ... for the E coli DnaE OB domain [12,16] The very N -terminal region of PolC and the C -terminal domain of DnaE appear to be specific for each type of polymerase The small a ⁄ b C -terminal domain of ... Interestingly, the first half of the PolC N -terminal region ( 1–100) also detected the PolIIIs-V domain, albeit weakly (hhsearch probability of 16%) The structures of PolIIIs-V and DnaA-I adopt a variant of...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... region of the 1H,15N-HSQC spectrum of the C -terminal domain of human eRF1 illustrating the presence of two conformational states of the protein Fig S2 Plot of the number and distribution of the ... between the HN of Thr358 and the Ha of His356 could be seen in both conformers (Fig 4; Fig S3) At the same time, the intensity of the NOE between the HN of Phe357 and the Ha of Lys354 in the open ... sizes of the relative populations and possibly the rate of conformer interconversion are sensitive to the environment of the domain For the great majority of the residues in the minidomain, the...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... importance of each of the negatively charged residues in the C -terminal extension in maintaining the solubility of the Hsp25 oligomer Similarly, the introduction of hydrophobicity into the C -terminal ... impaired when the polarity of the C -terminal extension is reduced [25], and introduction of hydrophobicity into the C -terminal extension of aA-crystallin results in immobilization of the C -terminal ... examined the role of residues in the C -terminal extensions of other sHsps, notably aA- and aB-crystallin, the flexible regions of these proteins are unique and distinct from that of Hsp25 The a-crystallins...
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Báo cáo khoa học: Effect of oculopharyngeal muscular dystrophy-associated extension of seven alanines on the fibrillation properties of the N-terminal domain of PABPN1 pot
... [26] The formation of amorphous aggregates was independent of the presence or length of the poly l-alanine sequence In contrast, no amorphous aggregates were observed with the N -terminal domain of ... considerably shorter in the case of the variant with the poly l-alanine extension [26] In this work, we further compare these two N -terminal fragments Differences on the level of fibril formation kinetics ... domain of PABPN1 consisting of amino acids 1–125 of the wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the fibrillation properties of the most extreme disease-associated...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... built from the X-ray structure of the C -terminal domain of Ure2p and integrates the finding that the N -terminal domain of Ure2p is flexible An enlargement of the region of Ure2p involved in the interaction ... conformational state of Ure2p as a result of the presence of the N -terminal domain of the protein that slightly differs from that adopted by its C -terminal moiety The only amino acid residue belonging to ... species, were observed in the presence of ATP No change in the mobility of the Ure2p–Ssa1p complexes was detected in the presence of ATP The stoichiometry of Ure2p and Ssa1p within the 120 and 160 kDa...
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Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt
... Effect of mutations in the C -terminal region of the RmInt1 IEP on RNA splicing in vivo We constructed a series of mutants to identify the functional features of the C -terminal region of the RmInt1 ... DIVa of the intron, whereas other regions of the RT and domain X interact with conserved catalytic core regions [12] Domain X is located in the position corresponding to the ‘thumb’ and part of the ... residue at the N-terminus of a-helix a4, the domain X contains the conserved amino acid residues HKXRA (RmInt1 residues 388–392) carrying a stretch of basic amino acids Some of the residues of the HKXRA...
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Báo cáo khoa học: The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 doc
... representation of the binding of the N -terminal domain (ND) of PEX1 to the peroxisomal membrane PEX1 is predicted to interact with the PEX6 portion of AAA domains PEX1-ND binds to the peroxisomal ... domains Fig Sequence comparison of the N -terminal domain (ND) of PEX1 with that of other related proteins (A) Schematic representation of the domain architecture of AAA-ATPases PEX6, PEX1, N-ethylmaleimide-sensitive ... in the presence of excess phytic acids, since phytic acid is not a perfect mimic of the headgroup of PtdIns4P Although PEX1-ND is recruited to the liposomes in the presence of a large excess of...
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Báo cáo Y học: Differential scanning calorimetric study of myosin subfragment 1 with tryptic cleavage at the N-terminal region of the heavy chain pdf
... These results are in favor of the above suggestion that the N -terminal region of myosin II is very important for structural stabilization of the entire motor domain of the myosin head The N -terminal ... result of the present work is that the N -terminal cleavage of the S1 heavy chain completely prevents the changes in the thermal unfolding of S1, i.e a significant increase in the protein thermal ... that of uncleaved S1 [29] In the present study, we applied the DSC approaches described above to examine the effects of N -terminal tryptic cleavage on the thermal unfolding of S1 The main goal of...
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RUNNIN’ WITH THE BIG DOGS The Long, Twisted History of the Texas-OU Rivalry docx
... WITH THE BIG DOGS and then the spirit of the people of the land took over Before long, and for decades then to come, anybody staying downtown the night before the Texas-OU game would drift off ... figured that the Longhorns were heading to Pasadena for the Rose Bowl, the granddaddy of them all, as it was regarded in those times Then a funny thing happened to the Longhorns on the way to the Rose ... his way from the fifty-yard line all the way down to the thirty-five, while the people screamed They didn’t generate the noise of the 1948 Oklahoma crowd, though, when they beat the Longhorns,...
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Measuring the long-term perception of monetary policy and the term structure docx
... expectations of each variable of the model The second stage is to include these endpoints in the autoregressive model for forecasting The use of these endpoints serves to anchor the long term forecast of ... movements in the perception of monetary policy by the public However, this demonstration is based only on the study of the stochastic process of in‡ ation and on the hypothesis that, in the long run, ... speci…cations for the long- term perception of the monetary authorities’in‡ ation target to explain, with the Fisher relationship, the presence of shifts in the long- term perception of the short-term...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... re-deposited under the same accession number Fig 1H–15N HSQC spectrum of the C -terminal domain of EW29 in the sugar-free state A 600 MHz 2D 1H–15N HSQC spectrum of the 0.9 mM C -terminal domain of EW29 at ... of the chemical shift titration data to the binding isotherm [27] A plot of the weighted average chemical-shift changes of 1H and 15N resonances for the cross-peaks of Gly122, as a function of ... subdomain c of EW29Ch was not observed; (b) the B-factor of the side chain of Lys105 was high, indicating that the side chain of Lys105 is flexible; (c) in this NMR study, the Kd of the c sugar-binding...
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Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx
... improving the solubility of the domain In the case of the AMPP protein, the boundary of the domain would have been better determined from an inspection of the structure rather than by the experimental ... ZnCl2 inhibits the activity of AMPP [1] Here, the presence of ZnCl2 in the dialysis buffer led to the precipitation of each of the three proteins Neither the intact protein nor the FEBS Journal ... tend to repel one another, thus destabilizing the protein For the native protein, the presence of the N -terminal domain and the oligomeric structure of C -terminal domain of E coli aminopeptidase...
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Báo cáo khoa học: Change in structure of the N-terminal region of transthyretin produces change in affinity of transthyretin to T4 and T3 pdf
... testing the hypothesis that the N -terminal regions of TTR affect the affinities of TTR for thyroid hormones A previous study revealed that altering the structure of the N -terminal region influenced the ... region of crocTTR was deleted The affinity of T3 and T4 to both TTRs was studied and the results are a powerful demonstration of the relationship between the evolution of the primary structure of the ... the N -terminal regions of TTR and the biological function of TTR 4014 Results Synthesis of recombinant human TTR In the crocodile, TTR is only synthesized in the liver during development in the...
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Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx
... also need to mention the mobility of the IIo forms of the different substrates The IIo form of the N -terminal 1–15 repeats was only slightly retarded relative to the position of the unphosphorylated ... differences in the extent of retardation of the IIo band in SDS/PAGE between the C -terminal and the N -terminal parts of the CTD (Figs 3A, 4A and 5) The first 15 CTD repeats only slightly change their mobility ... model depicting the possible action of the three kinases on the C -terminal domain (CTD) The amino acid sequence of the mouse CTD is shown at the bottom In the diagram, the heptad repeats, with...
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Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
... concerted DNA integration They show a strong structural role of the most C -terminal part of this C -terminal domain in the general folding of the enzyme They reinforce the role of the IN dimers, as a ... remove these contacts at the interface of the dimer However, the mutation decreases the number of intramolecular contacts within both monomers Y246 is located at the beginning of strand b3¢ The ... 3¢ end of the viral DNA In the second step (strand transfer), the hydroxyl groups at the 3¢ ends of the processed viral DNA attack a pair of phosphodiester bonds in the target DNA In the last...
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Báo cáo Y học: The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains pptx
... during the purification process Therefore small amounts of the DN-D4 and DC-D4 fragments were recovered N -terminal sequence analysis of the T2¢ and D4 fragments revealed that they have the expected ... investigate the state of the toxin fragments during membrane binding, we analyzed the susceptibilities of the T2¢ and D4 fragments to protease in the presence and absence of membranes (Fig 3) In the ... h-toxin C -terminal peptide In the lower panel the 3D structures of T2¢ and D4 are shown in black against a gray background of the whole h-toxin structure The arrow indicates the position of cleavage...
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