the legume family seed pod

... Unix There are several versions of Unix Until a few years ago, there were two main versions: the line of Unix releases that started at AT&T (the latest is System V Release 4), and another from the ... Our examples show the bash shell and the GNOME and KDE window environments We’ve chosen them because they’re popular and make good examples, not because we think they’re always the best.” If you ... first, and then break it into task-oriented sections Each section shows the best command to use for a task, explains what it does, and shows the syntax (how to put the command line together) The syntax...

Ngày tải lên: 21/12/2013, 11:15

... “opcode” of the instruction The opcode is the unique identifier of the instruction The next four bits, 1000, are the shift of the operand Then there is one bit (zero in this case) to signal that the direct ... used can be any of the registers in the register file The other major part of the CPU is the ALU The ALU can also take inputs from either the memory or c 1999 by CRC Press LLC the register file and ... and the repeat-end (RE) the end of the loop These registers are initialized automatically by the processor, but they are available to the user in case he needs to save them On the TMS320C30, there...

Ngày tải lên: 25/12/2013, 06:16

... to the validity of the Law of Large Numbers Are other theorems still valid, like the Central Limit or Large Deviation theorems? Those questions are usually related to the rates of mixing of the ... Here we consider the quadratic family, fa = a − x2 , where −1/4 ≤ a ≤ is the parameter, and we analyze its dynamics in the invariant interval The quadratic family has been one of the most studied ... time to time, we transfer the information from the phase space to the parameter, following the description of the parapuzzle nest which we will make in the next subsection The rules for this correspondence...

Ngày tải lên: 16/02/2014, 05:20

... and pathophysiology of the SREBP family H Shimano at G1 [19] In particular, p21 is a direct target of SREBP [20] The role of SREBP-1a in the regulation of cell growth and the cell cycle might be ... explains the physiological transcriptional regulation of energy storage in response to the nutritional status Under energy abun- dance scenarios, acetyl-CoA is used as a substrate for the synthesis ... involved in lipid synthesis during the cell cycle [14] Regulation of SREBP-1a in the cell cycle is mediated through its phosphorylation and ubiquitin-dependent degradation by the Fbw7 ubiquitin...

Ngày tải lên: 18/02/2014, 13:20

... remaining vs 50–60% of the membrane fraction These results are consistent with the idea that the Ga subunits are more stable in the membrane than in the cytoplasm To study this further we designed ... subunits with other proteins could also be responsible for the stabilization in the membrane The fact that the activated mutant forms of Gaq and Ga16 have the same behavior as the wild-type forms ... [35] Other proteins that interact with the Gaq subunits and may have a role in the stabilization of these subunits in the membrane are the regulators of G-protein signaling (RGS) [14] or the GRK2,...

Ngày tải lên: 20/02/2014, 03:20

... tested against CtBP in the yeast two-hybrid system Numbers indicate the amino acids in the Eos sequence, the filled rectangle indicates the position of the PEDLA motif, and the cross represents ... indicate the amino acids of the respective protein Filled rectangles indicate the position of the PXDLS-like motif, and the cross represents mutation of this motif (B) Yeast growth in the absence ... interaction (Fig 3A) Taken together these results suggest that the PEDLA motif in Eos is suboptimal and not the major determinant of CtBP binding, but that it and other sites within the N-terminus and C-terminus...

Ngày tải lên: 21/02/2014, 01:21

... factor of the Ets -family is involved in the regulation The members of the Ets -family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T and control the expression ... for C/EBP (CCAAT) is boxed The translation start site (ATG) and the cleavage site in the processing of the polypeptide (arrow) are also shown The nucleotide sequence of the 5¢-flanking region and ... the PCR reaction mixture in the absence of the cDNA library gave one band corresponding to that with the highest mobility (Fig 5, lane 2) Thus, we conclude that the most prominent band with the...

Ngày tải lên: 21/02/2014, 03:20

... b-cyclodextrin The top panel depicts the raw heat response for each b-cyclodextrin injection, and the bottom panel depicts the binding isotherm; open circles represent the integrated binding heat of the ... observed with the recombinant CBM45s suggests that the isolated SBD is destabilized as a result of the exposure of hydrophobic surface, which would otherwise be packed on other domains in the native ... pH 8.0 The full black line, grey line and broken black line are the thermograms of the first, second and third scans, respectively, at a rate of °CÆmin)1 AtAMY3 was expressed as either the full-length...

Ngày tải lên: 06/03/2014, 00:21

... along with the proximity of the charge relay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytic histidine The strength of the His290-Asp260 ... to 31% These structural homologs, which are members of the human HSL protein family, include the thermophilic esterase from Bacillus acidocaldarius [29] (44.2% of sequence identity), the thermophilic ... residues in the active pH range The imidazole ring of the catalytic His must be in a neutral state to capture the hydrogen of the catalytic serine for an efficient nucleophilic attack of the substrate...

Ngày tải lên: 07/03/2014, 16:20

... sequences in the same subfamily (scope: subfamily), between sequences in the same family, but not the same subfamily (scope: family) or between sequences in the same class, but not the same family ... Table shows the same analysis for the hits between the N- and C-terminal halves and vice versa within the OPA family, the AAT family, the ST3 families and the DAACS1 family In case of the AAT and ... ‘filtering’ of the blast searches strongly improves the specificity of the search (the ratio of true over false hits) but at the expense of much of the sensitivity of the search (the number of...

Ngày tải lên: 07/03/2014, 17:20

... the genus Giardia are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the earliest known branch of the cathepsin B family [23] Its phylogeny confirms that the cathepsin ... [4], and their comparable location enhances the significance of the sequence similarities Other functional amino acids in cathepsins are also conserved in most or all expansins First, the distribution ... concentrating the allergen according to the isoelectric point (pI) of the molecule A pH gradient from to 11 was established for the characterization of Phl p After completion of the run, the individual...

Ngày tải lên: 08/03/2014, 22:20

... contacts with their equivalents in the other subunit of the second dimer (Fig 4A) In the case of P21212 (TcRpiB-R5P), the four molecules in the asymmetric unit represent the tetramer The two active ... in the biological cycle of the parasite (i.e the epimastigote and the metacyclic trypomastigote in the insect vector, and the intracellular amastigote and the bloodstream trypomastigote in the ... for other complex structures The phosphate group lies at the same place, although the rest of the sugar is much less well defined The electron density suggests that All6P is bound primarily as the...

Ngày tải lên: 15/03/2014, 00:20

... above the relevant lanes, and the absence of any added protein is indicated by ()) The position of the free RNA and of the loading slots is indicated to the right of each gel A B abolished by the ... granules is shared by the MNAB protein encoded by the other member of the mammalian Rc3h gene family, Rc3h2 Stress granule localization is likely to be critical for the function of these proteins, ... whether the ROQ domain is involved in regulation of Icos expression, we used the pR-IRES–GFP retroviral vector [1], to express either RoquinWT, RoquinM199R or the Roquin construct lacking the...

Ngày tải lên: 15/03/2014, 11:20

... Representation of the full-length ERp57 structure with the region recognized as the x-linker colored in red (B) The N-terminal part of the x-linker folds against the b¢ domain in the structures of the ERp57 ... the b domain in addition to the residues in the b¢ domain, which form the majority of the binding site [16] In contrast to the bb¢ base, the catalytic domains in the available structures show ... Specifically, the side chains of Leu343 and Trp347, which are part of the linker between the b¢ and a¢ domains, are inserted into the PDI family of proteins hydrophobic cavity of the b¢ domain The binding...

Ngày tải lên: 15/03/2014, 23:20

... they in the EPHOPT complex (Fig 3) In addition, the backbone atoms of the Trp– Table Kinetic data The upper panel shows the effect of varying amino acids at the +2 position of the substrate The ... subtle, the phosphotyrosine at the +2 plays an important role in reordering the structure of the region of the C-terminal lobe that interacts with these substrates In the absence of the phosphate ... between the EphA3 kinase domain and the EPHOPT substrate not effect large conformational changes in the N- or C-terminal lobes of the kinase (Fig 2A) There is slight movement in the aF–aG loop in the...

Ngày tải lên: 16/03/2014, 02:20

... experimentally, whereas the results in the third column are those calculated from the V8 protease fragmentation of the gene products of Bcl-G and Ubi-L The fourth column indicates the number of the first and ... immunoblotting of the 33.5 kDa Ubi-L adduct using an antibody against synthetic peptide based on the sequence of Bcl-G The results of the Fig Primary structure of Bcl-G (A) The predicted amino ... bonds between the C terminal of the glycine residue of Ubi-L and the lysine of Bcl-G The 33.5 kDa Ubi-L adduct was digested by V8 protease and subjected to MALDI-MS analysis The data in the first column...

Ngày tải lên: 17/03/2014, 10:20

... modelling The aim of the present work was to study the diversity of trypsins in the digestive gland of P argus, with focus on: (a) the position of lobster trypsins within the brachyurins family ... the wall of the binding pocket and allow the access of bulky residues, like Arg and Lys, whereas the basic side chain of these residues is stabilized by Asp189 (chymotrypsin numbering) near the ... the enzyme, then the efficient protein digestion in these crustacea can be better explained, as well as their ecological success Some studies are available on decapod trypsins at the molecular...

Ngày tải lên: 23/03/2014, 03:20

... nonapeptide The nal residue of the resultant a-chain, Arg181, and the rst residue of the b-chain, Ala191, are also labelled (italics) The magenta colouring towards the bottom of the gure marks the position ... peptidase mechanisms [42,44] The study of the effect of pH on enzyme inactivation allows the calculation of the pKa of the His176 side chain involved in the inactivation reaction The rate of inactivation ... nitrogen atoms of the backbone of the residue preceding the catalytic Cys The conservation of this interaction is suggestive of its Fig The nal model of the clostripain catalytic domain The ribbon is...

Ngày tải lên: 23/03/2014, 12:20

... with the middle domain, which mediates the dimerization of HSP90, and the other, the identification of the minimal region of the C-terminal domain for the client binding Bearing in mind the fact ... essential for the dimerization was verified by the data shown in Fig On the other hand, the second assumption that the 35 amino acids were involved in the client binding was not true, but the central ... contrast, the combination failed to form a complex in HSP90a [17], but the combination of the middle-C-terminal domains either with the middle domain or the C-terminal domain is required for the interaction...

Ngày tải lên: 23/03/2014, 20:22

... intervals Therefore, the cleavage location is partially dependent on the incubation time of the substrate with the protease, at least within the limits of the experimental protocol Cleavage at the ... cannot exhibit the tertiary interactions that they establish in the intact proteins Therefore, the assessed conformational trends of isolated fragments are the same as those occurring in the protein ... the AGADIR algorithm [17] The locations of the eight helices (A–H) along the polypeptide chain of the protein are also indicated by boxes, according to the structural features obtained from the...

Ngày tải lên: 30/03/2014, 01:20